In Vitro Evaluation of Probiotic Properties of Lactic Acid Bacteria Isolated from Some Traditionally Fermented Ethiopian Food Products.
Authors: Mulaw G, Sisay Tessema T, Muleta D, Tesfaye A Abstract Probiotics are live microorganisms which when consumed in large number together with a food promote the health of the consumer. The aim of this study was to evaluate in vitro probiotic properties of lactic acid bacteria (LAB) isolated from traditional Ethiopian fermented Teff injera dough, Ergo, and Kocho products. A total of 90 LAB were isolated, of which 4 (4.44%) isolates showed 45.35-97.11% and 38.40-90.49% survival rates at pH values (2, 2.5, and 3) for 3 and 6 h, in that order. The four acid-tolerant isolates were found tolerant to 0.3% bi...
Source: International Journal of Microbiology - September 21, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Effect of Ethanol-Derived Clove Leaf Extract on the Oxidative Stress Response in Yeast Schizosaccharomyces pombe.
Authors: Fauzya AF, Astuti RI, Mubarik NR Abstract Compared to the widely explored antioxidant activity from the clove bud extract, less data are available regarding the potential pharmacological use of clove leaves. Our study aimed to explore the antioxidant activity of clove leaves extract in the cellular level. Thus, we used the yeast Schizosaccharomyces pombe as model organisms. Our data indicate that, following extract treatment (100 ppm), the viability of the stationary phase cells of S. pombe was higher than without extract and that of calorie restriction treatments. 100 ppm extract treatment a...
Source: International Journal of Microbiology - September 6, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antibiotic Use and Resistance Pattern in Ethiopia: Systematic Review and Meta-Analysis.
Conclusion and Recommendations: Inappropriate antibiotic use is a huge problem in Ethiopia, and many bacteria were resistant to commonly used antibiotics and similarly, multidrug-resistant bacterial strains are numerous. Appropriate antibiotic use should be ensured by prohibiting over-the-counter sale of antibiotics and strengthening antimicrobial stewardship. PMID: 31467550 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - September 1, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Effect of Prebiotics-Enhanced Probiotics on the Growth of Streptococcus mutans.
The objective of this study was to evaluate the efficacy of the prebiotics, galactooligosaccharides (GOS) and fructooligosaccharides (FOS), for enhancing the probiotic Lactobacillus acidophilus ATCC 4356, for inhibiting Streptococcus mutans (A32-2) for the prevention of dental caries. The growth rate of the S. mutans significantly decreased when cocultured with L. acidophilus in the GOS-supplemented medium at 3%, 4%, and 5%. In the FOS-supplemented medium, the growth rate of S. mutans significantly decreased in all concentrations when cocultured with L. acidophilus. There was no significant difference in the growth rate of...
Source: International Journal of Microbiology - September 1, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Analysis of Bacterial and Fungal Infections after Cytoreduction Surgery and Hyperthermic Intraperitoneal Chemotherapy: An Observational Single-Centre Study.
Discussion: The infection rate in our centre compared to previous studies of comparable patients was matching. Effective management of postoperative infections should be considered, and identified risk factors in this study can help to focus on effective prevention and treatment strategies. PMID: 31467552 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - September 1, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Extreme Environment Streptomyces: Potential Sources for New Antibacterial and Anticancer Drug Leads?
Authors: Sivalingam P, Hong K, Pote J, Prabakar K Abstract Antimicrobial resistance (AR) is recognized as one of the greatest threats to public health and in global concern. Consequently, the increased morbidity and mortality, which are associated with multidrug resistance bacteria, urgently require the discovery of novel and more efficient drugs. Conversely, cancer is a growing complex human disease that demands new drugs with no or fewer side effects. Most of the drugs currently used in the health care systems were of Streptomyces origin or their synthetic forms. Natural product researches from Streptomyces have ...
Source: International Journal of Microbiology - July 31, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Heterologous Ectoine Production in Escherichia coli: Optimization Using Response Surface Methodology.
Conclusion: This study is the first report on the expression of an ectoine gene cluster of Halomonas elongata BK-AG25 in E. coli BL21, under the control of the T7 promoter. Optimization of the level of nutrients in the medium, as well as the bioprocess condition using response surface methodology, has successfully increased the production of ectoine by the recombinant bacteria. PMID: 31354830 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - July 31, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Efficacy of Routine Fecal Microbiota Transplantation for Treatment of Recurrent Clostridium difficile Infection: A Retrospective Cohort Study.
Conclusion: Fecal instillation, for the treatment of relapsing CDI, is a promising approach, with 68% success rate reported in this study. The success rate of FMT is high, regardless of multiple comorbidities, extended use of antibiotics, or long time hospitalization. Although generally FMT is performed with fresh donor feces, our data show that the usage of frozen fecal culture could be an effective treatment alternative in recurrent CDI. PMID: 31354831 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - July 31, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Characterization of Clinical Isolates of Enterococci with Special Reference to Glycopeptide Susceptibility at a Tertiary Care Center of Eastern Nepal.
Conclusions: Enterococcus faecalis and E. faecium were the predominant species in causing enterococcal infections. The alarming rise in prevalence of vancomycin and multidrug resistance strains warrants immediate, adequate, and efficient surveillance program to prevent and control its spread. PMID: 31354832 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - July 31, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Synthesis of Silver Nanoparticles Using Aqueous Extract of Medicinal Plants' (Impatiens balsamina and Lantana camara) Fresh Leaves and Analysis of Antimicrobial Activity.
Authors: Aritonang HF, Koleangan H, Wuntu AD Abstract Plant-mediated synthesis of nanomaterials has been increasingly gaining popularity due to its eco-friendly nature and cost-effectiveness. In the present study, we synthesized silver (Ag) nanoparticles using aqueous extracts of fresh leaves of Impatiens balsamina and Lantana camara medicinal plants as bioreducing agents. This method allowed the synthesis of nanoparticles, which was confirmed by ultraviolet-visible (UV-Vis) spectrophotometry and transmission electron microscopy (TEM). UV-Vis spectra and visual observation showed that the color of the fresh leaf ex...
Source: International Journal of Microbiology - July 31, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Effectiveness of BOX-PCR in Differentiating Genetic Relatedness among Salmonella enterica Serotype 4,[5],12:i:- Isolates from Hospitalized Patients and Minced Pork Samples in Northern Thailand.
Authors: Poonchareon K, Pulsrikarn C, Nuanmuang N, Khamai P Abstract Salmonella enterica Serotype 4,[5],12:i:-, a monophasic variant of S. Typhimurium, with high virulence and multidrug resistance is distributed globally causing pathogenicity to both humans and domesticated animals. BOX-A1R-based repetitive extragenic palindromic-PCR (BOX)-PCR proved to be superior to three other repetitive element-based PCR typing methods, namely, enterobacterial repetitive intergenic consensus (ERIC)-, poly-trinucleotide (GTG)5-, and repetitive extragenic palindromic (REP)-PCR (carried out under a single optimized amplification c...
Source: International Journal of Microbiology - July 20, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antibacterial Potency of Honey.
Authors: Albaridi NA Abstract Despite the developments in controlling infectious disease around the world, they are still the second biggest cause of morbidity and mortality due in part to the increase in drug resistance among large numbers of the bacterial strains. This means that new strategies are needed to prevent and treat infectious disease. As a result, several ancient methods have been re-evaluated and the substances/procedures employed historically to cure diseases are now attracting renewed scientific attention. Honey is one such product that used to be widely used to combat bacteria. This review covers t...
Source: International Journal of Microbiology - July 11, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Accumulation of NH4 + and NO3 - inside Biofilms of Natural Microbial Consortia: Implication on Nutrients Seasonal Dynamic in Aquatic Ecosystems.
Authors: Kurniawan A, Yamamoto T Abstract Microbial biofilms are ubiquitous in aquatic ecosystems. Inside the biofilm is the nutrient-rich microenvironment promoted by the accumulation of the nutrient ions such as NH4 + and NO3 - from surrounding water. The present study investigated the characteristics of NH4 + and NO3 - accumulation into the biofilm of natural microbial consortia collected from Lake Biwa, Japan. The results showed the following: (1) the concentrations of NH4 + and NO3 - inside the biofilm were much higher than those in the surrounding water; (2) the nutrient ion concentration inside the biofilm c...
Source: International Journal of Microbiology - July 11, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Sensitivity of Heterotrophic Bacteria in the Low-Salinity Water Areas and Estuary in Siak District toward Pathogenic Bacteria in Fish.
Authors: Feliatra F, Hamdani R, Lukystyowati I, Nurachmi I Abstract The high rate of bacterial diseases in fishes and shrimps has lead scientists seek for natural antibiotic products that would act as a solution. An example of this product is the secondary metabolic products from heterotrophic bacteria. These bacteria could easily be found in many water regions and estuaries, including the Siak District, Riau, Indonesia. Therefore, this study aims at determining the ability of bacterial isolates in inhibiting the growth of pathogens (Vibrio alginolyticus, Aeromonas hydrophila, and Pseudomonas sp.). The research was...
Source: International Journal of Microbiology - July 11, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Mycobacterium bovis BCG Surface Antigens Expressed under the Granuloma-Like Conditions as Potential Inducers of the Protective Immunity.
Authors: Chen Y, Danelishvili L, Rose SJ, Bermudez LE Abstract Bovine tuberculosis (bTB) is a highly transmissible infection and remains of great concern as a zoonosis. The worldwide incidence of bTB is in rise, creating potential reservoir and increased infection risk for humans and animals. In attempts to identify novel surface antigens of Mycobacterium bovis as a proof-of-concept for potential inducers of protective immunity, we investigated surface proteome of M. bovis BCG strain that was cultured under the granuloma-like condition. We also demonstrated that the pathogen exposed to the biologically relevant env...
Source: International Journal of Microbiology - July 11, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Partial Characterization of Novel Bacteriocin SF1 Produced by Shigella flexneri and Their Lethal Activity on Members of Gut Microbiota.
Authors: Padilla C, Carrasco-Sánchez V, Padilla A, Lobos O Abstract A strain of Shigella flexneri producing bacteriocin was isolated from a patient with diarrhea. The main objective of this study was to isolate and partially characterize the bacteriocin. The producing microorganism was identified using biochemical, serological, and molecular methods. The lethal activity of the S. flexneri strain was studied using the drop method. This bacterial strain showed activity against different strains of E. coli and B. fragilis. Using immunological techniques, it was determined that S. flexneri belongs to serotype 2a...
Source: International Journal of Microbiology - June 16, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Determination of In Vitro Antimicrobial Activity of Five Sri Lankan Medicinal Plants against Selected Human Pathogenic Bacteria.
Conclusions: It can be concluded that E. divaricata and V. zizanioides crude ethanol, aqueous, and hexane extracts exhibited significant in vitro antibacterial activity against S. aureus, and the active compounds isolated from them can be potential sources for the synthesis of antibacterial drugs. PMID: 31198423 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - June 16, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Investigation of Bacteriological Quality of Meat from Abattoir and Butcher Shops in Bishoftu, Central Ethiopia.
Authors: Bersisa A, Tulu D, Negera C Abstract The study was conducted from November 2015 to November 2016 to determine bacterial load and identify pathogenic bacteria (S. aureus, E. coli, and Salmonellae species) in meat from abattoir and butcher shops as well as to assess associated hygienic and sanitation practices being experienced in the selected study site. A cross-sectional study was conducted where a simple random sampling method was used to select butcher shops, and the municipal abattoir was purposively selected. A structured questionnaire survey was also used to assess hygienic status of the municipal aba...
Source: International Journal of Microbiology - June 14, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antimicrobial Profiling of Bacteria Isolated from Fish Sold at Informal Market in Mufakose, Zimbabwe.
Authors: Gufe C, Canaan Hodobo T, Mbonjani B, Majonga O, Marumure J, Musari S, Jongi G, Makaya PV, Machakwa J Abstract The number of infections caused by antibiotic resistant bacteria is rising worldwide. Fish from multisource pollution waters can harbour multidrug-resistant bacteria that can be disseminated to humans through eating or contact of contaminated fish. A cross-sectional study was carried out to (i) isolate and phenotypically identify bacteria from 36 fish samples from informal market in Mufakose, Harare, and (ii) determine the antibiotic sensitivity pattern of the isolated bacteria against ten availabl...
Source: International Journal of Microbiology - June 14, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Encapsulation of Bifidobacterium pseudocatenulatum Strain G4 within Bovine Gelatin-Genipin-Sodium Alginate Combinations: Optimisation Approach Using Face Central Composition Design-Response Surface Methodology (FCCD-RSM).
Authors: Khalil KA, Mustafa S, Mohammad R, Ariff AB, Ahmad SA, Dahalan FA, Abdul Manap MY Abstract Bovine gelatin is a biopolymer which has good potential to be used in encapsulating matrices for probiotic candidate Bifidobacterium pseudocatenulatum strain G4 (G4) because of its amphoteric nature characteristic. Beads were prepared by the extrusion method using genipin and sodium alginate as a cross-linking agent. The optimisation of bovine gelatin-genipin-sodium alginate combinations was carried out using face central composition design (FCCD) to investigate G4 beads' strength, before and after exposed to simulate...
Source: International Journal of Microbiology - May 18, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Bacteriological Assessment of the Indoor Air of Different Hospitals of Kathmandu District.
This study was undertaken with an objective to determine the prevalence of nosocomial bacteria present in hospital indoor environment. A total of 16 air samples were taken from general wards and emergency wards of 8 different hospitals using an impactor air sampler in nutrient agar, mannitol salt agar, blood agar, cetrimide agar, and MacConkey agar. The bacteriological agents were isolated and identified by cultural characteristics, Gram staining, and biochemical tests, and their antibiotic susceptibility pattern was determined using CLSI Guideline, 2015. According to the European Union Guidelines to Good Manufacturing Pra...
Source: International Journal of Microbiology - May 17, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antibacterial Activity of Honey against Methicillin-Resistant Staphylococcus aureus: A Laboratory-Based Experimental Study.
Conclusions: Tested honey has both a bacteriostatic and bactericidal activity. Among the tested honey, "honey-2" had high antibacterial potency than others. PMID: 31073310 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - May 11, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Methicillin- and Inducible Clindamycin-Resistant Staphylococcus aureus among Patients with Wound Infection Attending Arba Minch Hospital, South Ethiopia.
Authors: Mama M, Aklilu A, Misgna K, Tadesse M, Alemayehu E Abstract Background: Wound infection is one of the most common hospital-acquired infections. Different bacteria cause infection, of which Staphylococcus aureus is one of the known bacteria in causing infection with increased drug-resistant isolates. Objective: To assess the prevalence and antimicrobial susceptibility pattern of methicillin and inducible clindamycin-resistant Staphylococcus aureus among patients with wound infections attending Arba Minch Hospital. Methods: A facility-based cross-sectional study was conducted from April to June 2017....
Source: International Journal of Microbiology - May 10, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Comparison of Serum Potassium, MagnEsium, and Calcium Levels between Kanamycin and Capreomycin-BASEd Regimen-Treated MultiDrug-Resistant TuBerculosis Patients in Bandung (CEASE MDR-TB): A Retrospective Cohort Study.
Authors: Soeroto AY, Darmawan G, Supriyadi R, Bhaskara PG, Santoso P, Alisjahbana B, Parwati I Abstract Treatment of multidrug-resistant tuberculosis (MDR-TB) with second-line injectable drugs may result in an electrolyte imbalance. This retrospective study was performed to compare and evaluate the effect of kanamycin and capreomycin on serum potassium, calcium, and magnesium in the first and second month treatment at a tertiary, top-referral hospital in Bandung, Indonesia. Data from 84 subjects with complete medical records of at least serum potassium during either kanamycin-based or capreomycin-based treatment we...
Source: International Journal of Microbiology - April 21, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Characterization and Synergistic Antimicrobial Evaluation of Lipopeptides from Bacillus amyloliquefaciens Isolated from Oil-Contaminated Soil.
Authors: Sudarmono P, Wibisana A, Listriyani LW, Sungkar S Abstract Lipopeptides show great potential for biomedical application. Several lipopeptides exhibit narrow and broad-spectrum inhibition activities. The aim of the study is to characterize the lipopeptides produced by B. amyloliquefaciens strain MD4-12 and evaluate the synergistic antimicrobial activity in combination with a conventional antibiotic against Gram-negative bacteria. B. amyloliquefaciens strain MD4-12 was isolated from oil-contaminated soil. The isolate was cultivated in McKeen medium, and the lipopeptides were isolated by precipitation and ext...
Source: International Journal of Microbiology - April 10, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Microbiota Landscape of Gut System of Guppy Fish (Poecilia reticulata) Plays an Outstanding Role in Adaptation Mechanisms.
Authors: Kayath CA, Ibala Zamba A, Goma-Tchimbakala J, Mamonékéné V, Mombo Makanga GM, Lebonguy AA, Nguimbi E Abstract Microbial consortium that is present in fish gut systems works together to achieve unknown specific roles. Here, we collected guppy fish from hydrocarbon- and trace metal-contaminated wastewater to assess the relationships between gut microbiota and host fish adaptation. Targeted genes and 16S rRNA amplicon sequencing have been used to identify gut bacteria of guppies. Mineral-conditioned medium contributes to identify bacteria with the ability to grow and/or to tolerate hydroc...
Source: International Journal of Microbiology - April 7, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Relationship of Sanitation Parameters with Microbial Diversity and Load in Raw Meat from the Outlets of the Metropolitan City Biratnagar, Nepal.
Authors: Mahato S Abstract The main aim of this study is to assess the microbial load of raw meat from outlets of Biratnagar and its relationship with several sanitation parameters. Samples were taken from meat outlets, and required microbiological procedures were followed as per guidelines. Approximately 63.6% of microbes were present in meat with poor sanitation while 36.4% were present in meat with good sanitation. Fungal contamination in poorly kept mutton was one and half times greater than chicken/mutton of good sanitation. Fungi such as Penicillium (21.3%), Mucor (16.3%), Aspergillus (15%), and Trichosporon ...
Source: International Journal of Microbiology - April 5, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Smear-Positive Tuberculosis Prevalence and Associated Factors among Pregnant Women Attending Antinatal Care in North Gondar Zone Hospitals, Ethiopia.
In this study, the prevalence of smear-positive tuberculosis was found to be higher than that in other community-based studies in the country. Therefore, health education, targeted screening of pregnant women for TB, and collaboration of TB-HIV clinic with antenatal care clinic should be implemented in the area. Further research should also be conducted for better understanding of the magnitude of tuberculosis in females of reproductive age. PMID: 30944569 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - April 5, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Prevalence of Bacterial Urinary Tract Infections and Associated Factors among Patients Attending Hospitals in Bushenyi District, Uganda.
This study was designed to determine the prevalence of UTI by isolating and characterizing the different bacterial etiological agents and to evaluate the factors associated with UTI. In this cross-sectional study, a total of 267, clean catch midstream urine (MSU) samples were collected aseptically and analyzed using standard microbiology methods. Data for the factors associated with UTI were obtained by use of questionnaires and standard laboratory tests for selected underlying conditions. The study revealed 86/267 (32.2%) UTI prevalence among patients attending hospitals in Bushenyi District, Uganda. Escherichia coli was ...
Source: International Journal of Microbiology - March 26, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Perchlorate-Reducing Bacteria from Hypersaline Soils of the Colombian Caribbean.
In conclusion, halophilic-isolated bacteria from hypersaline soils of the Colombian Caribbean are promising resources for the bioremediation of perchlorate contamination. PMID: 30906324 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - March 26, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Magnitude of Biofilm Formation and Antimicrobial Resistance Pattern of Bacteria Isolated from Urinary Catheterized Inpatients of Jimma University Medical Center, Southwest Ethiopia.
Authors: Awoke N, Kassa T, Teshager L Abstract Biofilm formation is one of the features of most bacteria. Catheterization in medicine is a source of highly resistant bacterial infections, and those bacteria respond poorly to antimicrobial therapy. Bacterial biofilm features were not described from catheterized inpatients in Ethiopia as its formation is known to afford antimicrobial resistance and challenge patient management. The aim of this study was to isolate catheter-associated urinary bacterial pathogens, their biofilm formation, and antimicrobial susceptibility pattern among inpatients of Jimma University Med...
Source: International Journal of Microbiology - March 19, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Screening and Antifungal Activity of a β-Carboline Derivative against Cryptococcus neoformans and C. gattii.
Conclusions: The synthetic β-carboline 8-nitroharmane significantly inhibits pathogenic Cryptococcus species and is interesting as a lead compound towards new therapy for Cryptococcus infections. PMID: 30805002 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - February 28, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Melittin Inhibition and Eradication Activity for Resistant Polymicrobial Biofilm Isolated from a Dairy Industry after Disinfection.
This study evaluated the ability of melittin to act as an antibacterial biofilm inhibitor and biofilm remover considering isolates from dairy industry. Minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), minimum biofilm inhibitory concentrations (MBICs), and biofilm removal activities were studied in polymicrobial biofilms produced from isolates. MIC and MBC were set at 1-3 µg/mL and 25-50 µg/mL for Gram-positive and Gram-negative bacteria, respectively. Results demonstrated a good MBIC reaching 85% inhibition ability and a good activity and better penetration in ...
Source: International Journal of Microbiology - February 16, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Isolation of Polygalacturonase-Producing Bacterial Strain from Tomatoes (Lycopersicon esculentum Mill.).
Conclusion: Microorganisms should be developed for large-scale production of enzymes in developing countries. PMID: 30766603 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - February 16, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Arsenic Resistance and Biosorption by Isolated Rhizobacteria from the Roots of Ludwigia octovalvis.
This study determines the minimum inhibitory concentration (MIC) of arsenic on identified rhizobacteria that were isolated from the roots of Ludwigia octovalvis (Jacq.) Raven. The arsenic biosorption capability of the was also analyzed. Among the 10 isolated rhizobacteria, five were Gram-positive (Arthrobacter globiformis, Bacillus megaterium, Bacillus cereus, Bacillus pumilus, and Staphylococcus lentus), and five were Gram-negative (Enterobacter asburiae, Sphingomonas paucimobilis, Pantoea spp., Rhizobium rhizogenes, and Rhizobium radiobacter). R. radiobacter showed the highest MIC of>1,500 mg/L of arsenic. All ...
Source: International Journal of Microbiology - February 8, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antibacterial Potential of Aloe weloensis (Aloeacea) Leaf Latex against Gram-Positive and Gram-Negative Bacteria Strains.
Conclusion: The results of the present investigation suggest that the leaf latex of A. weloensis can be used as potential leads to discover new drugs to control some bacterial infections. PMID: 30719044 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - February 6, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Magnitude of Vancomycin-Resistant Enterococci (VRE) Colonization among HIV-Infected Patients Attending ART Clinic in West Amhara Government Hospitals.
Conclusions: The observed VRE with multidrug resistance colonization need a periodic surveillance of antimicrobial testing to detect emerging resistance and prevent the spread of further drug resistance. PMID: 30693035 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - January 30, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Enterococcus faecium TIR-Domain Genes Are Part of a Gene Cluster Which Promotes Bacterial Survival in Blood.
This study identified a gene cluster, which includes genes encoding bacterial Toll/interleukin-1 receptor- (TIR-) domain-containing proteins (TirEs). The cluster was found to be unique to nosocomial strains and to be located on a putative mobile genetic element of phage origin. The three genes within the cluster appeared to be expressed as an operon. Expression was detected in bacterial culture media and in the presence of human blood. TirEs are released into the bacterial supernatant, and TirE2 is associated with membrane vesicles. Furthermore, the tirE-gene cluster promotes bacterial proliferation in human blood, indicat...
Source: International Journal of Microbiology - January 13, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Molecular Characterization of Low-Density Polyethene (LDPE) Degrading Bacteria and Fungi from Dandora Dumpsite, Nairobi, Kenya.
This study aimed at molecular and biochemical characterization of low-density polyethene (LDPE) degrading fungi and bacteria from Dandora dumpsite, Nairobi. Twenty bacterial and 10 fungal isolates were identified using 16S rDNA and 18S rDNA sequences for bacteria and fungi, respectively. The highest fungal degradation was attributed to Aspergillus oryzae strain A5,1 while the highest bacterial degradation was attributed to Bacillus cereus strain A5,a and Brevibacillus borstelensis strain B2,2, respectively. Isolates were screened for their ability to produce extracellular laccase and esterase; Aspergillus fumigatus strain ...
Source: International Journal of Microbiology - January 13, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Current Progress in the Structural and Biochemical Characterization of Proteins Involved in the Assembly of Lipopolysaccharide.
Authors: Bohl TE, Aihara H Abstract The lipid component of the outer leaflet of the outer membrane of Gram-negative bacteria is primarily composed of the glycolipid lipopolysaccharide (LPS), which serves to form a protective barrier against hydrophobic toxins and many antibiotics. LPS is comprised of three regions: the lipid A membrane anchor, the nonrepeating core oligosaccharide, and the repeating O-antigen polysaccharide. The lipid A portion is also referred to as endotoxin as its overstimulation of the toll-like receptor 4 during systemic infection precipitates potentially fatal septic shock. Because of the imp...
Source: International Journal of Microbiology - January 2, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Methicillin-Resistant Staphylococcus aureus (MRSA) in Artisanal Cheeses in M éxico.
In this study, the frequency of methicillin-resistant Staphylococcus aureus (MRSA) was 18.1% (8/44) and of enterotoxin A producers was 18.1% (8/44). When classified by biotypes, MRSA only belongs to the human ecovar biotype (2/8, 25%) and the D biotype (4/8, 50%). S. aureus producers of enterotoxin A were distributed in specific nonhost biotypes. PMID: 30581470 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - December 25, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Syphilis among Female Sex Workers: Results of Point-of-Care Screening during a Cross-Sectional Behavioral Survey in Burkina Faso, West Africa.
Conclusion: The prevalence of syphilis markers was high during this study among FSW. This highlights the need to reinforce the comprehensive preventive measures and treatment of syphilis in this population. PMID: 30532783 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - December 13, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antimicrobial Effects of Egyptian Local Chicory, Cichorium endivia subsp. pumilum.
Authors: Amer AM Abstract The discovery of novel and more efficient antimicrobial agents from natural sources like plants is one of the most important ways through which the growing threat of antibiotic-resistant pathogens can be overcome. Herein, we report the potential antimicrobial activity of Cichorium endivia L. subsp. pumilum. Different concentrations of various solvent extracts prepared from several parts of chicory were tested for their antimicrobial effect against a panel of microorganisms. The antimicrobial activity was analyzed using the well diffusion method, where zones of inhibition were used as indic...
Source: International Journal of Microbiology - December 13, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Characterization of Erythromycin and Tetracycline Resistance in Lactobacillus fermentum Strains.
Authors: Anisimova E, Yarullina D Abstract Lactobacillus fermentum colonizing gastrointestinal and urogenital tracts of humans and animals is widely used in manufacturing of fermented products and as probiotics. These bacteria may function as vehicles of antibiotic resistance genes, which can be transferred to pathogenic bacteria. Therefore, monitoring and control of transmissible antibiotic resistance determinants in these microorganisms is necessary to approve their safety status. The aim of this study was to characterize erythromycin and tetracycline resistance of L. fermentum isolates and to estimate the potent...
Source: International Journal of Microbiology - December 13, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Prevalence, Antimicrobial Susceptibility Pattern, and Associated Factors of Urinary Tract Infections among Adult Diabetic Patients at Metu Karl Heinz Referral Hospital, Southwest Ethiopia.
This study investigated the prevalence, antimicrobial susceptibility pattern of the isolates and associated factors of urinary tract infection among adult diabetic patients attending Metu Karl Heinz Referral Hospital, Southwest Ethiopia. An institutional-based cross-sectional study was conducted among 233 adult diabetic patients selected using simple random sampling technique. Data were collected using a structured questionnaire. Clean-catch midstream urine samples were investigated for the presence of pathogenic bacteria and their antimicrobial susceptibility pattern using recommended culture methods. Data were entered, c...
Source: International Journal of Microbiology - December 7, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Etiology of Coinfections in Children with Influenza during 2015/16 Winter Season in Nepal.
This study gives us better information on the respiratory pathogen profile and coinfection combinations which are important for diagnosis and treatment of ARIs. PMID: 30510579 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - December 5, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antifungal Susceptibility and Biofilm Production of Candida spp. Isolated from Clinical Samples.
Conclusion: Speciation of Candida isolates, detection of ability to form the biofilm, and monitoring of antifungal susceptibility testing are necessary for appropriate treatment. PMID: 30405717 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - November 10, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Bacterioplankton Activity in a Meso-eutrophic Subtropical Coastal Lagoon.
Authors: Schmitz Fontes ML, Fernandes H, Brandão M, Hennemann MC, Aparecida Loss R, de Souza Grinevicius VMA, Tonetta D, Cesca K, Hessel Silveira M, Bedin M, Recouvreux D, Vasconcellos Antônio R Abstract The aim of this study was to investigate whether the bacterioplankton activity in the meso-eutrophic Conceição Lagoon would increase significantly under allochthonous inputs of inorganic nutrients and organic carbon. Abundance and biomass of bacterioplankton were evaluated under three treatments: light (14 h light/10 h dark), complete darkness (dark-control), and nutrient (C...
Source: International Journal of Microbiology - November 8, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Evaluation of Bacillus Strains for Plant Growth Promotion and Predictability of Efficacy by In Vitro Physiological Traits.
The objectives of this study were to (1) identify Bacillus strains that can be consistent in promoting the growth of corn, wheat, and soybean and (2) determine whether physiological traits expressed in vitro can be predictive of growth promotion efficacy/consistency and be used for selecting effective strains. Twelve Bacillus strains isolated from wheat rhizospheres were evaluated in greenhouse pot tests with nonsterile soil for their effects on the growth of corn, soybean, and wheat. The strains also were assessed in vitro for multiple physiological traits. All 12 strains increased corn growth significantly compared to th...
Source: International Journal of Microbiology - November 8, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Prevalence and Antimicrobial Susceptibility of Bacterial Uropathogens Isolated from Pediatric Patients at Yekatit 12 Hospital Medical College, Addis Ababa, Ethiopia.
Conclusion: This study determined the prevalence of urinary tract infection in pediatric patients and highlighted the major bacterial uropathogens involved in UTI for the first time in the country. Furthermore, bacterial pathogen species and their frequency was consistent with the usually reported pattern, with E. coli being the most common organism isolated in cases of urinary tract infections followed by Klebsiella spp. Most of the bacterial isolates were multidrug resistant, and it is therefore suggested that appropriate antimicrobials should be administered to reduce the risk of multidrug resistant organisms developing...
Source: International Journal of Microbiology - November 4, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research