Characterization of Streptomyces Isolates Associated with Estuarine Fish Chanos chanos and Profiling of Their Antibacterial Metabolites-Crude-Extract.
This study investigated antibacterial-producing Streptomyces isolated from the gut of estuarine fish Chanos chanos, emphasizing screening for the producer of peptide-containing antibacterial compounds. Eighteen isolates were found during preliminary screening, in which four isolates showed the best antibacterial activities. Based on the morphological, physiological, and biochemical characterization, as well as 16S rRNA partial sequencing, all of the four isolates belonged to Streptomyces. Three isolates were suspected as novel isolate candidates based on homology presentations and phylogenetic tree analysis. Disk-diffusion...
Source: International Journal of Microbiology - October 10, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Genotypic Profiling of Bacillus cereus Recovered from Some Retail Foods in Ogun State, Nigeria, and Their Phylogenetic Relationship.
Authors: Adesetan TO, Efuntoye MO, Babalola OO Abstract Identifying Bacillus cereus with conventional methods is neither specific nor rapid because of the close relatedness of the B. cereus group, hence the need for molecular methods. Genotypic profiling of B. cereus isolates from food was obtained by Random Amplified Polymorphic DNA-polymerase chain reaction (RAPD-PCR) using OPR13 primer. A dendrogram was drawn with the Numerical Taxonomy System of Statistic (NTSYS) software. Thirty of the isolates were subjected to molecular identification by 16S rDNA sequencing. The thirty sequences were deposited in GenBank for...
Source: International Journal of Microbiology - October 4, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Urinary Calculi: A Microbiological and Biochemical Analysis at a Tertiary Care Hospital in Eastern Nepal.
Conclusions: The association of microorganism isolated from urine and nidus culture was significant that can predict the source of infective stone; however, in some cases, microorganisms and the antimicrobial susceptibility pattern from urine and nidus were different. This study emphasizes the use of appropriate antimicrobial agents to prevent the regrowth of residual stones and minimize the risk of infectious complications after surgical removal of stones. PMID: 33005194 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - October 4, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Detection and Profiling of Antibiotic Resistance among Culturable Bacterial Isolates in Vended Food and Soil Samples.
This study aimed at detecting and profiling antibiotic resistance of bacterial contaminants in vended food and the environment. Seventy antibiotic-resistant bacterial isolates were isolated from fried fish, African sausages, roasted meat, smokies, samosa, chips (potato fries), vegetable salads, and soil samples collected from Embu Town and Kangaru Market in Embu County, Kenya. The antibiotic susceptibility test, morphological and biochemical characterization, antibiosis assay, polymerase chain reaction-based detection of antibiotic resistance genes, and sequencing of the 16S rRNA gene were done. Analysis of variance on all...
Source: International Journal of Microbiology - September 25, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Prevalence of Escherichia coli in Under-Five Children with Diarrhea in Ethiopia: A Systematic Review and Meta-Analysis.
Authors: Zenebe T, Mitiku M, Alem Y Abstract Diarrhea remains as a high health burden, especially to children in low-income countries including Ethiopia. Diarrheagenic Escherichia coli have been commonly associated as bacterial pathogens causing diarrheal disease among children. This systemic review and meta-analysis was intended to determine the pooled prevalence of Escherichia coli in under-five children with diarrhea in Ethiopia. A comprehensive search in PubMed, Google Scholar, ScienceDirect, ResearchGate, and Google search engine and manual searching were done for this systematic review and meta-analysis. The ...
Source: International Journal of Microbiology - September 25, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Possible Role for Bacteriophages in the Treatment of SARS-CoV-2 Infection.
Authors: Mishra VN, Kumari N, Pathak A, Chaturvedi RK, Gupta AK, Chaurasia RN Abstract An outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in Wuhan City, China, in December 2019. Since then, the outbreak has grown into a global pandemic, and neither a vaccine nor a treatment for the disease, termed coronavirus disease 2019 (COVID-19), is currently available. The slow translational progress in the field of research suggests that a large number of studies are urgently required. In this context, this review explores the impact of bacteriophages on SARS-CoV-2, especially conce...
Source: International Journal of Microbiology - September 25, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Bacterial Profile among Patients with Suspected Bloodstream Infections in Ethiopia: A Systematic Review and Meta-Analysis.
Conclusion: The prevalence of bacterial isolates among presumptive patients suspected to BSIs in Ethiopia remains high. Furthermore, we found a remarkable variation in the pathogen distribution across the study setting. PMID: 32963541 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - September 25, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Morphological and Molecular Characterization of Toxigenic Aspergillus flavus from Groundnut Kernels in Kenya.
This study identified and further characterized aflatoxigenic A. flavus from groundnuts sampled from sundry shops in Kenya using integrated morphological and molecular approaches. The groundnuts were plated on potato dextrose agar for isolation and morphological observation of A. flavus based on macroscopic and microscopic features. Molecular characterization was done through amplification and comparison of the partial sequence of the ITS1-5.8S-ITS2 region. The expression analysis of aflR, aflS, aflD, aflP, and aflQ genes in the aflatoxin biosynthesis pathways was conducted to confirm the positive identification of A. flav...
Source: International Journal of Microbiology - September 25, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Molecular Serotyping and Antibiotic Resistance Patterns of Escherichia coli Isolated in Hospital Catering Service in Morocco.
Authors: Ghita BT, Bennani L, Berrada S, Benboubker M, Bennani B Abstract Escherichia coli is related to foodborne disease and outbreaks worldwide. It mainly affects persons at high risk as newborns, infants, and individuals with impaired immune system in hospitals. Multidrug-resistant E. coli is currently spreading both in community and hospital settings. Our study aims to evaluate the presence of E. coli and the incidence of its antibiotic resistance in samples obtained from various cooked and raw foods (N = 300), food contact surfaces (N = 238), and food handlers (N = 40...
Source: International Journal of Microbiology - September 15, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Genetic Diversity and Virulence Factors of S. aureus Isolated from Food, Humans, and Animals.
In this study, a wide genetic diversity of strains of S. aureus from different foods, humans, and animals was found. This demonstrates evolution, genetic versatility, and, therefore, the adaptation of this microorganism in different environments. PMID: 32908519 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

The Role of Bacterial Biofilm in Antibiotic Resistance and Food Contamination.
Authors: Abebe GM Abstract Biofilm is a microbial association or community attached to different biotic or abiotic surfaces or environments. These surface-attached microbial communities can be found in food, medical, industrial, and natural environments. Biofilm is a critical problem in the medical sector since it is formed on medical implants within human tissue and involved in a multitude of serious chronic infections. Food and food processing surface become an ideal environment for biofilm formation where there are sufficient nutrients for microbial growth and attachment. Therefore, biofilm formation on these su...
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Molecular Detection of Panton Valentine Leukocidin Toxin in Clinical Isolates of Staphylococcus aureus from Kiambu County, Kenya.
This study shows that PVL positive isolates occur in the sampled hospitals in the county and female as well as children must be taken into consideration among patients with wound infections when isolating S. aureus. PMID: 32908521 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Fungi and Aflatoxin Levels in Traditionally Processed Cassava (Manihot esculenta Crantz) Products in Homa Bay County, Kenya.
This study assessed the levels of fungi and aflatoxin contamination in traditionally processed cassava products (Akuoga and Abeta). A total of 38 samples were collected from the local markets in 7 subcounties in Homa Bay County, Kenya. The levels of aflatoxin were determined using an indirect competitive ELISA protocol. Yeast and mould contamination was determined using ISO 21527-2 method. Mean aflatoxin levels in chopped, fermented, and sun-dried cassava (Akuoga) were 0.36 μg/kg compared to 0.25 μg/kg in chopped and sun-dried (Abeta) products. Aflatoxin contamination was detected in 55% of the samples ...
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Dual Role of Bacteria in Carcinoma: Stimulation and Inhibition.
Authors: Al-Hilu SA, Al-Shujairi WH Abstract Although what unifies the carcinogenic microorganisms has not been determined by multiple studies, the role of bacteria in the development of neoplasms has not been properly elucidated. In this review, we discuss links between the bacterial species and cancer, with focus on immune responses for the stimulation of tumor cells such as induction of inflammation. Finally, we will describe the potential therapeutic strategies of bacteria on target tumors to improve treatment while mitigating adverse reactions. Cancer is a series of genetic changes that transform normal cells ...
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Genomic and Pathogenic Characteristics of Virulent Newcastle Disease Virus Isolated from Chicken in Live Bird Markets and Backyard Flocks in Kenya.
This study reports the first complete genome sequences of NDV from backyard chicken in Kenya. The results showed that all three isolates are virulent, as assessed by the mean death time (MDT) and intracerebral pathogenicity index (ICPI) in specific antibody negative (SAN) embryonated eggs and 10-day-old chickens, respectively. Also, the polybasic amino acid sequence at the fusion-protein cleavage site had the motif 112RRQKRFV118. Histopathological findings in four-week-old SPF chicken challenged with the NDV isolates KE001, KE0811, and KE0698 showed multiple organ involvement at five days after infection with severe effect...
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antibiotics Resistance and Biofilm Formation Capacity of Staphylococcus spp. Strains Isolated from Surfaces and Medicotechnical Materials.
The objective of this study is to evaluate the susceptibility to antibiotics and the biofilm formation capacity of staphylococci species isolated from surfaces and medicotechnical materials at the university hospital center of Abomey-Calavi/Sô-Ava in Benin. Samples were collected according to ISO/DIS14698-1 standard from the surfaces and medicotechnical materials by the dry swab method. The isolation of Staphylococcus strains was performed on Chapman agar, and their identification was performed using microscopic and biochemical methods. The susceptibility of Staphylococcus isolates to antibiotics was evaluated by the...
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Prevalence and Antimicrobial Susceptibility Patterns of Neisseria gonorrhoeae among Suspected Patients Attending Private Clinics in Jimma, Ethiopia.
Conclusion: The prevalence of gonococcal infection is high. In the current study, participants who have no information about sexually transmitted infection were more likely to be infected by N. gonorrhoeae. According to our study, ciprofloxacin is effective against gonococcal infection. PMID: 32908526 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antibacterial Potential of Jatropha sp. Latex against Multidrug-Resistant Bacteria.
Conclusions: The latex of J. curcas, J. gossypilofia Linn., and J. multifida has the potential to be developed as antibacterial agents, especially against MRSA and CRPA strain, but further in vivo research and discovery of the mode of its action are required to shed the light on the effects. PMID: 32908527 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Molecular Identification and Antimicrobial Potential of Streptomyces Species from Nepalese Soil.
In this report, 18 Streptomyces species isolated from the soil were reported based on sequence analysis of 16S rRNA. Among them, 12 isolates have shown antibacterial activity against extended-spectrum beta-lactamase- (ESBL-) producing Escherichia coli. Here, we have also analyzed 16S rRNA in 27 Streptomyces species whose whole-genome sequence is available, which has revealed that some species have multiple copies of the 16S gene (∼1.5 kb) with significant variation in nucleotides. In contrast, some Streptomyces species shared identical DNA sequences in multiple copies of 16S rRNA. The sequencing of numerous copies of 1...
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Differential Expression and PAH Degradation: What Burkholderia vietnamiensis G4 Can Tell Us?
Authors: Cauduro GP, Leal AL, Lopes TF, Marmitt M, Valiati VH Abstract Petroleum is the major energy matrix in the world whose refining generates chemical byproducts that may damage the environment. Among such waste, polycyclic aromatic hydrocarbons (PAH) are considered persistent pollutants. Sixteen of these are considered priority for remediation, and among them is benzo(a)pyrene. Amid remediation techniques, bioremediation stands out. The genus Burkholderia is amongst the microorganisms known for being capable of degrading persistent compounds; its strains are used as models to study such ability. High-throughpu...
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Molecular and Immunological Diagnostic Techniques of Medical Viruses.
Authors: Reta DH, Tessema TS, Ashenef AS, Desta AF, Labisso WL, Gizaw ST, Abay SM, Melka DS, Reta FA Abstract Viral infections are causing serious problems in human population worldwide. The recent outbreak of coronavirus disease 2019 caused by SARS-CoV-2 is a perfect example how viral infection could pose a great threat to global public health and economic sectors. Therefore, the first step in combating viral pathogens is to get a timely and accurate diagnosis. Early and accurate detection of the viral presence in patient sample is crucial for appropriate treatment, control, and prevention of epidemics. Here, we s...
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Immunoinformatic Analysis to Identify Proteins to Be Used as Potential Targets to Control Bovine Anaplasmosis.
Authors: Rodríguez-Camarillo SD, Quiroz-Castañeda RE, Aguilar-Díaz H, Vara-Pastrana JE, Pescador-Pérez D, Amaro-Estrada I, Martínez-Ocampo F Abstract Omics sciences and new technologies to sequence full genomes provide valuable data that are revealed only after detailed bioinformatic analysis is performed. In this work, we analyzed the genomes of seven Mexican Anaplasma marginale strains and the data from a transcriptome analysis of the tick Rhipicephalus microplus. The aim of this analysis was to identify protein sequences with predicted features to be used as potential targets t...
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Modulation of Gut Microbiota Profile and Short-Chain Fatty Acids of Rats Fed with Taro Flour or Taro Starch.
Authors: Surono IS, Venema K Abstract To investigate the effect of flour and starch of the Indonesian native tuber "taro" on the composition and activity of the gut microbiota in diabetic rats, streptozotocin (STZ)-induced diabetic rats were fed normal chow (AIN), or AIN in which corn starch was replaced by either taro flour or purified taro starch for 4 weeks. Fecal samples were collected at baseline and after 4 weeks, and the composition of microbial communities was measured using 16S rRNA sequencing, while SCFAs were measured using ion chromatography. Bodyweight declined upon DM induction with STZ. Fee...
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

In Vitro Modulator Effect of Total Extract from the Endophytic Paenibacillus polymyxa RNC-D in Leishmania (Leishmania) amazonensis and Macrophages.
In this study, the total extract produced by the endophytic Paenibacillus polymyxa RNC-D was used to evaluate the leishmanicidal, nitric oxide, and cytokines production using RAW 264.7 macrophages. The results showed that, in the leishmanicidal assay with L. amazonensis, EC50 values at the periods of 24 and 48 hours were 0.624 mg/mL and 0.547 mg/mL, respectively. Furthermore, the cells treated with the extract presented approximately 25% of infected cells with an average of 3 amastigotes/cell in the periods of 24 and 48 hours. Regarding the production of cytokines in RAW 264.7 macrophages infected/treated wit...
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Evaluation of the Antibacterial Activity of Pleurotus spp. Cultivated on Different Agricultural Wastes in Chiro, Ethiopia.
Authors: Gashaw G, Fassil A, Redi F Abstract In the present study, mushrooms, Pleurotus ostreatus and Pleurotus florida, were cultivated on different agricultural wastes namely coffee straw (CS), pea straw (PS), Sorghum Grain Residue (SGR), and Wheat Grain (WG) for the evaluation of antibacterial activity. Antimicrobial activity evaluation was carried out against human pathogenic microorganisms, namely, Escherichia coli, Bacillus subtilis, Streptococcus faecalis, Pseudomonas aeruginosa, and Salmonella typhi by using the disc diffusion method. Methanolic extracts of P. ostreatus cultivated on a Sorghum grain residue...
Source: International Journal of Microbiology - September 12, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Application of Microbial Bioenzymes in Soil Stabilization.
Authors: Mekonnen E, Kebede A, Tafesse T, Tafesse M Abstract Soil stabilization is a mechanical or chemical alteration of one or more soil properties to create an improved soil material possessing the desired engineering properties. The aim of this article was to review bioenzyme-based soil stabilization techniques with an emphasis on bioenzymes production, mechanism of soil stabilization and future challenges, and opportunities of the sector. Soils are stabilized to increase strength and durability or to prevent erosion and dust generation. Cost-effective soil stabilization technology has been a fundamental part o...
Source: International Journal of Microbiology - August 26, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Probiotic Potential of Lactic Acid Starter Cultures Isolated from a Traditional Fermented Sorghum-Millet Beverage.
Authors: Byakika S, Mukisa IM, Byaruhanga YB, Muyanja C Abstract The purpose of this study was to establish the probiotic potential of lactic acid bacteria (LAB) starter cultures, Lb. plantarum MNC 21, L. lactis MNC 24, and W. confusa MNC 20, isolated from a traditionally fermented sorghum-millet beverage from Uganda. The cultures were examined for tolerance to acid and bile salts, bile salt hydrolase (BSH) activity, antibiotic susceptibility, biogenic amine production, mucin degradation, hydrophobicity, auto-aggregation, adherence to the ileum, coaggregation, and antimicrobial properties against selected pathogeni...
Source: International Journal of Microbiology - August 26, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Fungal Variation during Peanut Paste Storage.
The objective of this study is to evaluate the level of fungal contamination of peanut paste produced according to different moulding processes during storage. Thirty samples of peanut paste were produced from 60 kg of peanut pods according to three types of moulding (domestic moulding, artisanal moulding, and hygienic moulding) and then preserved for three months. These thirty samples were subjected to microbiological analysis using the conventional mould count method. The moisture content of the various peanut pastes was determined according to the AOAC method. Fungi were identified by using taxonomic schemes base...
Source: International Journal of Microbiology - August 26, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Risk Factors Associated with Prevalence of Candida albicans, Gardnerella vaginalis, and Trichomonas vaginalis among Women at the District Hospital of Dschang, West Region, Cameroon.
Conclusion: Drastic measures should be taken in order to improve life styles to regress the frequency of these infections. Results obtained in this study, will help to educate and shed more light on the prevalence of vaginal infections in the West Region of Cameroon. PMID: 32831846 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - August 26, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Characterisation of Vibrio Species from Surface and Drinking Water Sources and Assessment of Biocontrol Potentials of Their Bacteriophages.
Authors: Maje MD, Kaptchouang Tchatchouang CD, Manganyi MC, Fri J, Ateba CN Abstract The aim of this study was to characterise Vibrio species of water samples collected from taps, boreholes, and dams in the North West province, South Africa, and assess biocontrol potentials of their bacteriophages. Fifty-seven putative Vibrio isolates were obtained on thiosulfate-citrate-bile-salt-sucrose agar and identified using biochemical tests and species-specific PCRs. Isolates were further characterised based on the presence of virulence factors, susceptibility to eleven antibiotics, and biofilm formation potentials. Twenty-...
Source: International Journal of Microbiology - August 26, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Microencapsulation of Probiotic Strains by Lyophilization Is Efficient in Maintaining the Viability of Microorganisms and Modulation of Fecal Microbiota in Cats.
This study aimed to (i) evaluate the effects of two types of microencapsulation techniques, different encapsulating agents, and 120 days of storage on the viability of a commercial probiotic product and (ii) investigate fecal microbiota populations and fecal characteristics of adult cats fed with diets supplemented with probiotics. Three experimental treatments were evaluated: T1, commercial feed (control); T2, commercial kibbles coated with probiotics; and T3, commercial feed supplemented with freeze-dried probiotics and fructooligosaccharides. Fructooligosaccharides and gum arabic were used as encapsulating agents for fr...
Source: International Journal of Microbiology - August 19, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Functional Gene Diversity of Selected Indigenous Hydrocarbon-Degrading Bacteria in Aged Crude Oil.
Authors: Ehis-Eriakha CB, Chikere CB, Akaranta O Abstract Crude oil pollution has consistently deteriorated all environmental compartments through the cycle of activities of the oil and gas industries. However, there is a growing need to identify microbes with catabolic potentials to degrade these pollutants. This research was conducted to identify bacteria with functional degradative genes. A crude oil-polluted soil sample was obtained from an aged spill site at Imo River, Ebubu, Komkom community, Nigeria. Bacteria isolates were obtained and screened for hydrocarbon degradation potential by turbidometry assay. Pla...
Source: International Journal of Microbiology - August 19, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Impact of Sequential Passaging on Protein Expression of E. coli Using Proteomics Analysis.
Authors: Alhajouj MS, Alsharif GS, Mirza AA Abstract Urinary tract infection (UTI) is one of the most prevalent bacterial infections in the world affecting the bladder and the kidney. Escherichia coli (E. coli) is the main causative agent of 80-90% of community-acquired UTIs, about 40% of nosocomial UTIs, and 25% of recurrent UTIs. The field of proteomics has emerged as a great tool to analyze expressed proteins to identify possible biomarkers associated with many pathological states and, to the same extent, those associated with bacterial pathogenesis and their ability to cause recurrent infections. Here, in a des...
Source: International Journal of Microbiology - August 19, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Characterization of Staphylococcus aureus Strains Isolated from Veterinary Hospital.
This study aims to detect Staphylococcus aureus (S. aureus) resistance in the veterinary hospital environment. S. aureus are one of the components of the microbiota, and they may be present in patients in a veterinary hospital environment. Methicillin resistance is determined by a chromosomal gene (mecA), which codes for modifications in the beta-lactam antibiotic receptor, where the penicillin-binding protein will have a low affinity for the antibiotic. Samples were collected through swabs of materials and equipment at the hospital. S. aureus was identified in 7.6% (21/276) of the samples collected, and of the 21 strains ...
Source: International Journal of Microbiology - August 19, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Cultivable Actinobacteria First Found in Baikal Endemic Algae Is a New Source of Natural Products with Antibiotic Activity.
According to this study and previously published materials, diversity of cultivable actinobacteria and rare strains isolated from D. baicalensis is comparable to that of cultivable actinobacteria previously isolated from plant sources of Lake Baikal. Also, it exceeds the cultivable actinobacteria diversity previously described for macroinvertebrates, water, or sediments of Lake Baikal. The large number of rare and active strains associated with the endemic alga D. baicalensis could be the promising sources for biopharmaceutical and biotechnological developments and discovery of new natural compounds. PMID: 32802070 [Pu...
Source: International Journal of Microbiology - August 19, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Corrigendum to "In Vitro Evaluation of Probiotic Properties of Lactic Acid Bacteria Isolated from Some Traditionally Fermented Ethiopian Food Products".
Corrigendum to "In Vitro Evaluation of Probiotic Properties of Lactic Acid Bacteria Isolated from Some Traditionally Fermented Ethiopian Food Products". Int J Microbiol. 2020;2020:6401356 Authors: Mulaw G, Sisay Tessema T, Muleta D, Tesfaye A Abstract [This corrects the article DOI: 10.1155/2019/7179514.]. PMID: 32802071 [PubMed - in process] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - August 19, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Bacterial Composition, Community Structure, and Diversity in Apis nigrocincta Gut.
This study is the first report on the gut microbial community structure and composition of A. nigrocincta from Indonesia. PMID: 32802072 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - August 19, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Endophytes: Colonization, Behaviour, and Their Role in Defense Mechanism.
Authors: Mengistu AA Abstract Biotic and abiotic factors cause an enormous amount of yield and economical loss. However, endophytes can play a significant role in enhancing the tolerance of plants. Endophytes systematically colonize different parts of the host, but plants use a variety of defense mechanisms towards microbial infection. However, they have to survive the oxidative environments, and endophytes like Enterobacter sp. encode superoxide dismutases, catalases, and hydroperoxide reductases to cope up the oxidative stress during colonization. On the contrary, others produce subtilomycin which binds with flag...
Source: International Journal of Microbiology - August 19, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antimycobacterial and PknB Inhibitory Activities of Venezuelan Medicinal Plants.
In this study, we evaluated 118 extracts from 58 Venezuelan plant species for their ability to inhibit the growth of Mycobacterium tuberculosis mc26020, using the agar dilution method. Additionally, we determined the ability of these extracts to inhibit the activity of PknB protein, an essential M. tuberculosis serine/threonine kinase, using a high-throughput luminescent assay. Of the 118 extracts tested, 14 inhibited bacterial growth with a minimum inhibitory concentration ≤500 μg/ml, and 36 inhibited the kinase activity with a half-maximal inhibitory concentration
Source: International Journal of Microbiology - August 19, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Detection of bla NDM-1 Encoding Imepenemase among the Imipenem-Resistant Gram-Negative Bacilli Isolated from Various Clinical Samples at a Tertiary Care Hospital of Eastern Nepal: A Descriptive Cross-Sectional Study.
Conclusion: A high percentage of the NDM-1 producer was noted among imipenem-resistant GNB. Apart from performing only antimicrobial sensitivity test, phenotypic and molecular screening should be employed to find out the actual number of metallo-β-lactamase producers and the existence of the resistance gene. PMID: 32802075 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - August 19, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Aspergillus nidulans: A Potential Resource of the Production of the Native and Heterologous Enzymes for Industrial Applications.
Authors: Kumar A Abstract Aspergillus nidulans is a filamentous fungus that is a potential resource for industrial enzymes. It is a versatile fungal cell factory that can synthesize various industrial enzymes such as cellulases, β-glucosidases, hemicellulases, laccases, lipases, proteases, β-galactosidases, tannases, keratinase, cutinases, and aryl alcohol oxidase. A. nidulans has shown the potential to utilize low-cost substrates such as wheat bran, rice straw, sugarcane bagasse, rice bran, coir pith, black gram residue, and chicken feathers to produce enzymes cost-effectively. A. nidulans has also been ...
Source: International Journal of Microbiology - August 19, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antibiotic and Disinfectant Susceptibility Patterns of Bacteria Isolated from Farmed Fish in Kirinyaga County, Kenya.
Authors: Wanja DW, Mbuthia PG, Waruiru RM, Bebora LC, Ngowi HA, Nyaga PN Abstract Fish bacterial pathogens cause diseases which result in a considerable economic impact on the aquaculture industry, necessitating the use of antimicrobials for their control. However, intensive and indiscriminate use of antimicrobials has led to increased occurrence of drug resistance in pathogenic bacteria, as well as normal flora. The aim of the current study was to determine the susceptibility patterns of bacteria isolated from fish, with respect to some commonly used antibiotics and disinfectants. Bacteria were isolated between De...
Source: International Journal of Microbiology - August 19, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Asymptomatic Bacteriuria among Pregnant Women Attending Antenatal Care at Hiwot Fana Specialized University Hospital, Harar, Eastern Ethiopia: Magnitude, Associated Factors, and Antimicrobial Susceptibility Pattern.
Conclusion: The overall prevalence of asymptomatic bacteriuria among pregnant women in this study was high. Direction of wiping after genital wash, catheterization, and postcoital urination increases the odds of asymptomatic bacteriuria. Therefore, health education on the predisposing factors is strongly recommended. PMID: 32765608 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - August 13, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

The Improvement of Bioethanol Production by Pentose-Fermenting Yeasts Isolated from Herbal Preparations, the Gut of Dung Beetles, and Marula Wine.
Authors: Moremi ME, Jansen Van Rensburg EL, La Grange DC Abstract Efficient conversion of pentose sugars to ethanol is important for an economically viable lignocellulosic bioethanol process. Ten yeasts fermenting both D-xylose and L-arabinose were subjected to an adaptation process with L-arabinose as carbon source in a medium containing acetic acid. Four Meyerozyma caribbica-adapted strains were able to ferment L-arabinose to ethanol in the presence of 3 g/L acetic acid at 35°C. Meyerozyma caribbica Mu 2.2f fermented L-arabinose to produce 3.0 g/L ethanol compared to the parental strain with 1.0...
Source: International Journal of Microbiology - August 13, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

MCR-1 Gene Encoded Colistin-Resistant Escherichia coli in Raw Chicken Meat and Bean Sprouts in Malaysia.
This study was conducted to detect the presence of colistin-resistant Escherichia coli (E. coli) in raw chicken meat and bean sprouts collected from local markets and to determine the antimicrobial resistance patterns of the E. coli isolates. A total of 100 samples, comprised of 50 raw chicken meat and 50 bean sprouts, were collected and processed. Kirby-Bauer method was used to determine the antimicrobial resistance patterns, and PCR amplification was used to detect E. coli species-specific and colistin resistance (mcr-1 and mcr-2) genes. The results showed that 52.1% (12/23) of the E. coli isolated from raw chicken meat ...
Source: International Journal of Microbiology - August 13, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Magnitude and Its Associated Factors of Urinary Tract Infection among Adult Patients Attending Tigray Region Hospitals, Northern Ethiopia, 2019.
Authors: Hailay A, Zereabruk K, Mebrahtom G, Aberhe W, Bahrey D Abstract Background: Urinary tract infection is a major public health problem in terms of morbidity and mortality worldwide. It ranks as the number one infection which leads to an antibiotic prescription after a physician's visit. However, there are limited studies done on UTI in Ethiopia. Hence, this study was aimed to assess the magnitude of urinary tract infection and its associated factors among adult patients attending hospitals of the Tigray region, Ethiopia. Methods and Material. A hospital-based cross-sectional study was conducted from April to...
Source: International Journal of Microbiology - August 13, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Reprocessing of Contaminated MGIT 960 Cultures to Improve Availability of Valid Results for Mycobacteria.
Authors: Subramanyam B, Sivaramakrishnan G, Sangamithrai D, Ravi R, Thiruvengadam K, Vijayaragavan V, Selvaraju A, Tripathy SP, Mondal R Abstract Optimal recovery of mycobacteria from the contaminated liquid culture is a challenge. While alternative methods have been suggested to reduce the rate of contamination in the BACTEC MGIT 960 system, reprocessing the contaminated liquid culture improves recovery of Mycobacterium tuberculosis. Among 793 MGIT cultures raised from as many sputum specimens after primary decontamination by the standard NaLC-NaOH method, valid results were available for 687 (86.6%) as 106 (13.4%...
Source: International Journal of Microbiology - August 2, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Evaluation of Sociodemographic Factors among Diabetic Patients with Urinary Tract Infections in Kisii Referral Hospital, Kenya.
The objective of this study was to determine the association between sociodemographic factors and UTI among diabetics. The study was carried out in the Kisii Teaching and Referral Hospital in Kenya. One hundred and eighty diabetic patients were enrolled in cross-sectional study design. Clean-catch midstream urine was collected from all participants and cultured in cysteine lactose electrolyte deficient agar for bacterial isolation. Classification of a positive culture for urinary tract infection was based on more than 100,000 (≥105) colony-forming units of a single bacterial species. The data were analyzed using frequencies, chi-square (p
Source: International Journal of Microbiology - July 30, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Isolation and Identification of Some Probiotic Bacteria and Their Potential Role in Improving Immune Response and Resistance of Nile Tilapia (Oreochromis niloticus) in Comparison with a Commercial Product.
Authors: Abomughaid MM Abstract This work aimed to retrieve a field isolate of probiotic from Nile tilapia (Oreochromis niloticus) and compare the obtained results with a commercial probiotic product through experimental studies. The study was conducted on 250 Nile tilapia. Ten fish were used to isolate the probiotic strain. Two isolates showed an in vitro inhibitory effect against pathogenic A. hydrophila. The isolate with the largest zone was identified by PCR. Sixty fish were used to test the safety of a potential probiotic. One hundred and eighty fish were used in a two-month feeding experiment. Fish were divid...
Source: International Journal of Microbiology - July 30, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antibiotic Susceptibility, Clonality, and Molecular Characterization of Carbapenem-Resistant Clinical Isolates of Acinetobacter baumannii from Washington DC.
This study revealed the emergence of VIM carbapenemase-producing A. baumannii isolates, which has not been previously reported in the United States. PMID: 32695174 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - July 23, 2020 Category: Microbiology Tags: Int J Microbiol Source Type: research