Arsenic Resistance and Biosorption by Isolated Rhizobacteria from the Roots of Ludwigia octovalvis.
This study determines the minimum inhibitory concentration (MIC) of arsenic on identified rhizobacteria that were isolated from the roots of Ludwigia octovalvis (Jacq.) Raven. The arsenic biosorption capability of the was also analyzed. Among the 10 isolated rhizobacteria, five were Gram-positive (Arthrobacter globiformis, Bacillus megaterium, Bacillus cereus, Bacillus pumilus, and Staphylococcus lentus), and five were Gram-negative (Enterobacter asburiae, Sphingomonas paucimobilis, Pantoea spp., Rhizobium rhizogenes, and Rhizobium radiobacter). R. radiobacter showed the highest MIC of>1,500 mg/L of arsenic. All ...
Source: International Journal of Microbiology - February 8, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antibacterial Potential of Aloe weloensis (Aloeacea) Leaf Latex against Gram-Positive and Gram-Negative Bacteria Strains.
Conclusion: The results of the present investigation suggest that the leaf latex of A. weloensis can be used as potential leads to discover new drugs to control some bacterial infections. PMID: 30719044 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - February 6, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Magnitude of Vancomycin-Resistant Enterococci (VRE) Colonization among HIV-Infected Patients Attending ART Clinic in West Amhara Government Hospitals.
Conclusions: The observed VRE with multidrug resistance colonization need a periodic surveillance of antimicrobial testing to detect emerging resistance and prevent the spread of further drug resistance. PMID: 30693035 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - January 30, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Enterococcus faecium TIR-Domain Genes Are Part of a Gene Cluster Which Promotes Bacterial Survival in Blood.
This study identified a gene cluster, which includes genes encoding bacterial Toll/interleukin-1 receptor- (TIR-) domain-containing proteins (TirEs). The cluster was found to be unique to nosocomial strains and to be located on a putative mobile genetic element of phage origin. The three genes within the cluster appeared to be expressed as an operon. Expression was detected in bacterial culture media and in the presence of human blood. TirEs are released into the bacterial supernatant, and TirE2 is associated with membrane vesicles. Furthermore, the tirE-gene cluster promotes bacterial proliferation in human blood, indicat...
Source: International Journal of Microbiology - January 13, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Molecular Characterization of Low-Density Polyethene (LDPE) Degrading Bacteria and Fungi from Dandora Dumpsite, Nairobi, Kenya.
This study aimed at molecular and biochemical characterization of low-density polyethene (LDPE) degrading fungi and bacteria from Dandora dumpsite, Nairobi. Twenty bacterial and 10 fungal isolates were identified using 16S rDNA and 18S rDNA sequences for bacteria and fungi, respectively. The highest fungal degradation was attributed to Aspergillus oryzae strain A5,1 while the highest bacterial degradation was attributed to Bacillus cereus strain A5,a and Brevibacillus borstelensis strain B2,2, respectively. Isolates were screened for their ability to produce extracellular laccase and esterase; Aspergillus fumigatus strain ...
Source: International Journal of Microbiology - January 13, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Current Progress in the Structural and Biochemical Characterization of Proteins Involved in the Assembly of Lipopolysaccharide.
Authors: Bohl TE, Aihara H Abstract The lipid component of the outer leaflet of the outer membrane of Gram-negative bacteria is primarily composed of the glycolipid lipopolysaccharide (LPS), which serves to form a protective barrier against hydrophobic toxins and many antibiotics. LPS is comprised of three regions: the lipid A membrane anchor, the nonrepeating core oligosaccharide, and the repeating O-antigen polysaccharide. The lipid A portion is also referred to as endotoxin as its overstimulation of the toll-like receptor 4 during systemic infection precipitates potentially fatal septic shock. Because of the imp...
Source: International Journal of Microbiology - January 1, 2019 Category: Microbiology Tags: Int J Microbiol Source Type: research

Methicillin-Resistant Staphylococcus aureus (MRSA) in Artisanal Cheeses in M éxico.
In this study, the frequency of methicillin-resistant Staphylococcus aureus (MRSA) was 18.1% (8/44) and of enterotoxin A producers was 18.1% (8/44). When classified by biotypes, MRSA only belongs to the human ecovar biotype (2/8, 25%) and the D biotype (4/8, 50%). S. aureus producers of enterotoxin A were distributed in specific nonhost biotypes. PMID: 30581470 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - December 25, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Syphilis among Female Sex Workers: Results of Point-of-Care Screening during a Cross-Sectional Behavioral Survey in Burkina Faso, West Africa.
Conclusion: The prevalence of syphilis markers was high during this study among FSW. This highlights the need to reinforce the comprehensive preventive measures and treatment of syphilis in this population. PMID: 30532783 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - December 13, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antimicrobial Effects of Egyptian Local Chicory, Cichorium endivia subsp. pumilum.
Authors: Amer AM Abstract The discovery of novel and more efficient antimicrobial agents from natural sources like plants is one of the most important ways through which the growing threat of antibiotic-resistant pathogens can be overcome. Herein, we report the potential antimicrobial activity of Cichorium endivia L. subsp. pumilum. Different concentrations of various solvent extracts prepared from several parts of chicory were tested for their antimicrobial effect against a panel of microorganisms. The antimicrobial activity was analyzed using the well diffusion method, where zones of inhibition were used as indic...
Source: International Journal of Microbiology - December 13, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Characterization of Erythromycin and Tetracycline Resistance in Lactobacillus fermentum Strains.
Authors: Anisimova E, Yarullina D Abstract Lactobacillus fermentum colonizing gastrointestinal and urogenital tracts of humans and animals is widely used in manufacturing of fermented products and as probiotics. These bacteria may function as vehicles of antibiotic resistance genes, which can be transferred to pathogenic bacteria. Therefore, monitoring and control of transmissible antibiotic resistance determinants in these microorganisms is necessary to approve their safety status. The aim of this study was to characterize erythromycin and tetracycline resistance of L. fermentum isolates and to estimate the potent...
Source: International Journal of Microbiology - December 13, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Prevalence, Antimicrobial Susceptibility Pattern, and Associated Factors of Urinary Tract Infections among Adult Diabetic Patients at Metu Karl Heinz Referral Hospital, Southwest Ethiopia.
This study investigated the prevalence, antimicrobial susceptibility pattern of the isolates and associated factors of urinary tract infection among adult diabetic patients attending Metu Karl Heinz Referral Hospital, Southwest Ethiopia. An institutional-based cross-sectional study was conducted among 233 adult diabetic patients selected using simple random sampling technique. Data were collected using a structured questionnaire. Clean-catch midstream urine samples were investigated for the presence of pathogenic bacteria and their antimicrobial susceptibility pattern using recommended culture methods. Data were entered, c...
Source: International Journal of Microbiology - December 7, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Etiology of Coinfections in Children with Influenza during 2015/16 Winter Season in Nepal.
This study gives us better information on the respiratory pathogen profile and coinfection combinations which are important for diagnosis and treatment of ARIs. PMID: 30510579 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - December 5, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antifungal Susceptibility and Biofilm Production of Candida spp. Isolated from Clinical Samples.
Conclusion: Speciation of Candida isolates, detection of ability to form the biofilm, and monitoring of antifungal susceptibility testing are necessary for appropriate treatment. PMID: 30405717 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - November 10, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Bacterioplankton Activity in a Meso-eutrophic Subtropical Coastal Lagoon.
Authors: Schmitz Fontes ML, Fernandes H, Brandão M, Hennemann MC, Aparecida Loss R, de Souza Grinevicius VMA, Tonetta D, Cesca K, Hessel Silveira M, Bedin M, Recouvreux D, Vasconcellos Antônio R Abstract The aim of this study was to investigate whether the bacterioplankton activity in the meso-eutrophic Conceição Lagoon would increase significantly under allochthonous inputs of inorganic nutrients and organic carbon. Abundance and biomass of bacterioplankton were evaluated under three treatments: light (14 h light/10 h dark), complete darkness (dark-control), and nutrient (C...
Source: International Journal of Microbiology - November 8, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Evaluation of Bacillus Strains for Plant Growth Promotion and Predictability of Efficacy by In Vitro Physiological Traits.
The objectives of this study were to (1) identify Bacillus strains that can be consistent in promoting the growth of corn, wheat, and soybean and (2) determine whether physiological traits expressed in vitro can be predictive of growth promotion efficacy/consistency and be used for selecting effective strains. Twelve Bacillus strains isolated from wheat rhizospheres were evaluated in greenhouse pot tests with nonsterile soil for their effects on the growth of corn, soybean, and wheat. The strains also were assessed in vitro for multiple physiological traits. All 12 strains increased corn growth significantly compared to th...
Source: International Journal of Microbiology - November 8, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Prevalence and Antimicrobial Susceptibility of Bacterial Uropathogens Isolated from Pediatric Patients at Yekatit 12 Hospital Medical College, Addis Ababa, Ethiopia.
Conclusion: This study determined the prevalence of urinary tract infection in pediatric patients and highlighted the major bacterial uropathogens involved in UTI for the first time in the country. Furthermore, bacterial pathogen species and their frequency was consistent with the usually reported pattern, with E. coli being the most common organism isolated in cases of urinary tract infections followed by Klebsiella spp. Most of the bacterial isolates were multidrug resistant, and it is therefore suggested that appropriate antimicrobials should be administered to reduce the risk of multidrug resistant organisms developing...
Source: International Journal of Microbiology - November 4, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Evaluation of Antimicrobial Activity of Four Organic Acids Used in Chicks Feed to Control Salmonella typhimurium: Suggestion of Amendment in the Search Standard.
Authors: El Baaboua A, El Maadoudi M, Bouyahya A, Belmehdi O, Kounnoun A, Zahli R, Abrini J Abstract Today, the general public has become increasingly aware of salmonellosis problems. Organic acids are known by their antimicrobial potential and commonly used for improving the quality of poultry feed. In this context, the present work evaluated the inhibitory effect of four organic acids, namely, acetic acid, citric acid, lactic acid, and tartaric acid, at different levels of contamination by Salmonella typhimurium. The neutralization of these organic acids in vitro and in the presence of one-day-old chick's organs ...
Source: International Journal of Microbiology - October 28, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antimicrobial Activity of Piper marginatum Jacq and Ilex guayusa Loes on Microorganisms Associated with Periodontal Disease.
Conclusion: Piper marginatum Jacq and Ilex guayusa Loes total ethanol extracts and fractions presented outstanding antimicrobial activity against P. gingivalis ATCC 33277, P. intermedia ATCC 25611, and F. nucleatum ATCC 25586. PMID: 30356383 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - October 26, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Characterization of Pathogenic Bacteria Isolated from Sudanese Banknotes and Determination of Their Resistance Profile.
This study was conducted to assess the prevalence of contamination by pathogenic bacteria in Sudanese banknotes, determine the susceptibility of the isolated organisms towards commonly used antibiotics, and detect some antibiotic resistance genes. Methods: This study was carried out using 135 samples of Sudanese banknotes of five different denominations (2, 5, 10, 20, and 50 Sudanese pounds), which were collected randomly from hospitals, food sellers, and transporters in all three districts of Khartoum, Bahri, and Omdurman. Bacterial prevalence was determined using culture-based techniques, and their sensitivity patter...
Source: International Journal of Microbiology - October 24, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Risk Factors for Vaginal Colonization and Relationship between Bacterial Vaginal Colonization and In-Hospital Outcomes in Women with Obstructed Labor in a Ugandan Regional Referral Hospital.
Conclusion: There was no difference in maternal or neonatal morbidity between women with vaginal colonization with E. coli, GBS, and Enterococcus and those who were not colonized. Duration of labor was associated with increased risk of vaginal colonization in women with obstructed labor. PMID: 30327672 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - October 19, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Prevalence of Multidrug Resistance and Extended-Spectrum β-Lactamase Carriage of Clinical Uropathogenic Escherichia coli Isolates in Riyadh, Saudi Arabia.
This study aims at determining the antimicrobial susceptibility patterns of a collection of clinical E. coli urine isolates and evaluating the ESBL carriage of these isolates at phenotypic and genotypic levels. A total of 100 E. coli urine isolates were collected at a tertiary healthcare centre in Riyadh from January 2018 to March 2018. Antimicrobial susceptibility testing was carried out for all isolates. ESBL production was characterized at phenotypic and genotypic levels using double-disc synergy test and polymerase chain reaction, respectively. Detection of different ESBL variants was performed using DNA sequencing. Of...
Source: International Journal of Microbiology - October 12, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Health Care Workers in Tertiary and Regional Hospitals in Dar es Salam, Tanzania.
In conclusion, we found a high nasal carriage of MRSA and resistance to commonly prescribed antimicrobial agents among HCWs. Implementation of infection control measures including contact precautions, urgent reporting of MRSA laboratory results, and routine MRSA screening of HCWs is highly needed to reduce MRSA spreading. PMID: 30275835 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - October 4, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Erratum to "Production of Biosurfactants by Soil Fungi Isolated from the Amazon Forest".
Erratum to "Production of Biosurfactants by Soil Fungi Isolated from the Amazon Forest". Int J Microbiol. 2018;2018:1586859 Authors: Holanda Sena H, Alves Sanches M, Fernando Silva Rocha D, Oliva Pinto Filho Segundo W, de Souza ÉS, de Souza JVB Abstract [This corrects the article DOI: 10.1155/2018/5684261.]. PMID: 30258462 [PubMed - in process] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - September 28, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Prevalence of Multidrug-Resistant Escherichia coli Isolated from Drinking Water Sources.
This study therefore sought to document the microbiological quality and antibiograms of bacterial isolates (E. coli strains) from six different water sources in order to determine their safety for human consumption and to provide updated antibiotic data for pragmatic treatment of patients. Bacteria isolation and identification was done using API and conventional methods. Antibiotic susceptibility testing was conducted using the Kirby-Bauer method. Results obtained indicated that all the water sources tested were of poor quality. Bacteria isolated included E. coli, Enterobacter spp., Klebsiella spp., Salmonella typhi, Strep...
Source: International Journal of Microbiology - September 15, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Molecular Characterization of Newcastle Disease Virus from Backyard Poultry Farms and Live Bird Markets in Kenya.
This study points to possible circulation of NDV of similar genetic characteristics between backyard poultry farms and live bird markets in Kenya. The study also suggests the possible spread of velogenic NDV between Kenya and Uganda possibly through cross-border live bird trade. Our study provides baseline information on the genetic characteristics of NDV circulating in the Kenyan poultry population. This highlights the need for the ND control programmes to place more stringent measures on cross-border trade of live bird markets and poultry products to prevent the introduction of new strains of NDV that would otherwise be ...
Source: International Journal of Microbiology - August 30, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Corrigendum to "Surfaces and Air Bacteriology of Selected Wards at a Referral Hospital, Northwest Ethiopia: A Cross-Sectional Study".
Corrigendum to "Surfaces and Air Bacteriology of Selected Wards at a Referral Hospital, Northwest Ethiopia: A Cross-Sectional Study". Int J Microbiol. 2018;2018:2190787 Authors: Getachew H, Derbie A, Mekonnen D Abstract [This corrects the article DOI: 10.1155/2018/6413179.]. PMID: 30151011 [PubMed - in process] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - August 29, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Vancomycin-Resistant Enterococci and Its Associated Risk Factors among HIV-Positive and -Negative Clients Attending Dessie Referral Hospital, Northeast Ethiopia.
Conclusion: The high prevalence of VRE and multidrug-resistant enterococci in this study signals the emergence of VRE. Detection of VRE in this study indicates decreased antibiotic treatment options of multidrug-resistant enterococci. Therefore, there should be a need to perform continuous surveillance, rational use of antibiotics, and more detailed study using phenotypic and genotypic methods. PMID: 30123274 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - August 22, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Coffea canephora Peptides in Combinatorial Treatment with Fluconazole: Antimicrobial Activity against Phytopathogenic Fungus.
In this study, it was shown that Cc-LTP2 and Cc-GRP in combination with fluconazole were able to inhibit the growth of the fungus F. solani, to promote permeabilization of its membrane, and to induce the production of ROS, suggesting a combinatorial activity between the peptides and fluconazole. PMID: 30123275 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - August 22, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Gastrointestinal Pathobionts in Pediatric Crohn's Disease Patients.
Authors: Purcell RV, Kaakoush NO, Mitchell HM, Pearson JF, Keenan JI Abstract Crohn's disease (CD) is an inflammatory disease of the gastrointestinal tract, with a rising incidence worldwide, particularly in children. CD is thought to arise due to an immune response to environmental factors. The role of bacteria in CD has recently been highlighted, and here, we examine the prevalence of two bacterial species, enterotoxigenic Bacteroides fragilis (ETBF) and Fusobacterium nucleatum, implicated in gastrointestinal pathologies, in a pediatric CD cohort. Stool samples from 30 children with treatment-naïve CD and 30...
Source: International Journal of Microbiology - August 22, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Antistaphylococcal and Antibiotic Resistance Modulatory Activities of Thirteen Cameroonian Edible Plants against Resistant Phenotypes.
Conclusion: The present study demonstrated that the 13 tested plants had antistaphylococcal effects and that DES, HEL, UGL, and UGB could be used in combination with EPI to combat resistance to Staphylococcus aureus. Also, it demonstrated that some studied extracts and mostly RHL could be used as antibiotic resistance modulators to fight against resistant strains of S. aureus. PMID: 30057614 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - July 31, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Triazole-Resistant Aspergillus fumigatus from Fungicide-Experienced Soils in Naivasha Subcounty and Nairobi County, Kenya.
Authors: Kemoi EK, Nyerere A, Bii CC Abstract The mainstay in prevention and treatment of aspergillosis is the use triazole drugs. In Kenya, the use of agricultural azole is one of the predisposing factors in development of resistance. One hundred fifty-six (156) experienced soils were collected from agricultural farms and cultured on Sabouraud DextroseAagar. The study isolated 48 yielded Aspergillus fumigatus and 2 A. flavus. All the isolates were subjected to antifungal susceptibility testing against three triazoles: posaconazole, voriconazole, and itraconazole. Out of the isolates, 3 had MIC of 32 and 1 had MIC ...
Source: International Journal of Microbiology - July 29, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Bacterial Profile and Antimicrobial Susceptibility Pattern of the Isolates from Stethoscope, Thermometer, and Inanimate Surfaces of Mizan-Tepi University Teaching Hospital, Southwest Ethiopia.
Conclusion: S. aureus, CoNS, and E. coli were the predominant isolates. Most isolates showed highest susceptibility to ciprofloxacin and least to ampicillin and penicillin. There is no regular sanitation and disinfection of hospital equipment and surfaces. PMID: 30050575 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - July 29, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Modification of Medium Composition for Enhancing the Production of Antifungal Activity from Xenorhabdus stockiae PB09 by Using Response Surface Methodology.
This study demonstrated that the RSM and CCD were shown to be valuable tools for optimizing the culture medium that maximize the antifungal activity of X. stockiae PB09. PMID: 30008748 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - July 17, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Patterns of Change in Metabolic Capabilities of Sediment Microbial Communities in River and Lake Ecosystems.
This study establishes a close linkage between physical and chemical properties (temperature and organic matter content) of lake and river sediments and associated microbial functional activities. PMID: 29977299 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - July 7, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Retracted: Prevalence of Bacteriuria and Antimicrobial Susceptibility Patterns among Diabetic and Nondiabetic Patients Attending at Debre Tabor Hospital, Northwest Ethiopia.
Authors: Microbiology IJO Abstract [This retracts the article DOI: 10.1155/2017/5809494.]. PMID: 29977300 [PubMed - in process] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - July 7, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Genomic Variability among Field Isolates and Laboratory-Adapted Strains of Leptospira borgpetersenii Serovar Hardjo.
Authors: Llanes A, Restrepo CM, Riesgo-Ferreiro P, Rajeev S Abstract Leptospira borgpetersenii serovar Hardjo colonizes cattle kidneys and may occasionally infect humans and other mammals. Strains belonging to two clonal subtypes (types A and B) with marked differences in their pathogenicity in the hamster experimental model have been described for this serovar. Such differences have been attributed to point mutations in individual genes, although those genes have not yet been characterized. In order to better understand genetic variability among L. borgpetersenii serovar Hardjo isolates, we sequenced and compared ...
Source: International Journal of Microbiology - June 30, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Comparison of the Antimicrobial Efficacy of Various Contact Lens Solutions to Inhibit the Growth of Pseudomonas aeruginosa and Staphylococcus aureus.
According to this study, the solutions named "Biotrue" and "Aquasoft" met the primary stand-alone and reached the 3log reduction and 5log reduction criteria in the manufacturer recommended time, respectively. No conclusion could be drawn for Opti-Free Replenish since the minimum recommended disinfection time was overnight, whereas it was noted for 6 hr only, and it should have been experimented further. The effectiveness of multipurpose solutions varies against different bacterial species. We have observed that the antimicrobial activity of different solutions varies with respect to time of incub...
Source: International Journal of Microbiology - June 30, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Isolates, Isolated from a Burn Hospital in Southwest Iran in 2006 and 2014.
Conclusion: The predominance of ST-239 in this region during the last eight years is a major concern. It also has a disturbing impact on the management of staphylococcal infections. Moreover, the SCCmec type IV strain is able to disseminate rapidly in hospital environments, demanding an improvement in infection-control policy. PMID: 29887890 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - June 13, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Surfaces and Air Bacteriology of Selected Wards at a Referral Hospital, Northwest Ethiopia: A Cross-Sectional Study.
Conclusions: This study showed high degree of bacterial load that is beyond the standard limits on both surfaces and air samples of the hospital. Furthermore, some 75% of the isolates were found multidrug resistant. Therefore, it is important to evaluate and strengthen the infection prevention practice of the hospital. Moreover, stakeholders should also reinforce actions to decrease the pressure of antimicrobial resistance in the studied area. PMID: 29861733 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - June 6, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Epidemiological Survey of Rotaviruses Responsible for Infantile Diarrhea by the Immunomolecular Technique in Cotonou (Benin, West Africa).
Authors: Agbla JMM, Capo-Chichi A, Agbankpé AJ, Dougnon TV, Yadouleton AWM, Houngbégnon O, Glele-Kakai C, Armah GE, Bankolé H Abstract Rotavirus remains the main causative agent of gastroenteritis in young children, in countries that have not yet introduced the vaccine. Benin, in order to implement the WHO recommendations, projects to introduce the rotavirus vaccine in 2018 as part of its Expanded Program on Immunization. But before the introduction of this vaccine, epidemiological data on rotavirus infections and rotavirus genotypes circulating in Benin should be available. The aim of this stu...
Source: International Journal of Microbiology - June 3, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Production of Biosurfactants by Soil Fungi Isolated from the Amazon Forest.
Authors: Sena HH, Sanches MA, Rocha DFS, Segundo WOPF, de Souza ÉS, de Souza JVB Abstract Biosurfactants are surface-active compounds that have sparked interest in recent years because of their environmental advantages over conventional surfactants. The aim of this study was to investigate the production of biosurfactants by soil fungi isolated from the Amazon forest. Fungi colonies were isolated from soil samples and screened for biosurfactant production in submerged fermentation. In addition, the influences of bioprocess factors (carbon source, nitrogen source, pH, and fermentation time) were investigated....
Source: International Journal of Microbiology - June 3, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Illumina MiSeq Sequencing for Preliminary Analysis of Microbiome Causing Primary Endodontic Infections in Egypt.
Authors: Tawfik SA, Azab MM, Ahmed AAA, Fayyad DM Abstract The use of high throughput next generation technologies has allowed more comprehensive analysis than traditional Sanger sequencing. The specific aim of this study was to investigate the microbial diversity of primary endodontic infections using Illumina MiSeq sequencing platform in Egyptian patients. Samples were collected from 19 patients in Suez Canal University Hospital (Endodontic Department) using sterile # 15K file and paper points. DNA was extracted using Mo Bio power soil DNA isolation extraction kit followed by PCR amplification and agarose gel ele...
Source: International Journal of Microbiology - June 2, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Prevalence of Helicobacter pylori cagA and iceA Genes and Their Association with Gastrointestinal Diseases.
In conclusion, cagA and iceA genes could be used as markers for severe gastrointestinal diseases. iceA gene was strongly related to cagA gene. PMID: 29849647 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - June 2, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Brown Rot-Type Fungal Decomposition of Sorghum Bagasse: Variable Success and Mechanistic Implications.
Authors: Presley GN, Ndimba BK, Schilling JS Abstract Sweet sorghum is a promising crop for a warming, drying African climate, and basic information is lacking on conversion pathways for its lignocellulosic residues (bagasse). Brown rot wood-decomposer fungi use carbohydrate-selective pathways that, when assessed on sorghum, a grass substrate, can yield information relevant to both plant biomass conversion and fungal biology. In testing sorghum decomposition by brown rot fungi (Gloeophyllum trabeum, Serpula lacrymans), we found that G. trabeum readily degraded sorghum, removing xylan prior to removing glucan. Serpu...
Source: International Journal of Microbiology - June 2, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Molecular Typing and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus Isolated from Bovine Milk in Tanzania.
This study was performed to determine the molecular characteristics and antimicrobial susceptibility pattern of MRSA strains isolated from raw bovine milk sold at local markets in Tanzania. A total of 117 raw milk samples were cultured on Baird-Parker medium to isolate S. aureus and PCR was used for amplification of gltB gene for S. aureus identification and the presence of mecA gene for methicillin-resistant strains. Coagulase-negative (CN) S. aureus were reconfirmed using tube coagulase, DNase, and API Staph tests. MRSA isolates were spa typed whereas antimicrobial susceptibility testing was performed by the disc diffusi...
Source: International Journal of Microbiology - May 4, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Prevalence of Bacterial Vaginosis and Its Association with Risk Factors among Nonpregnant Women: A Hospital Based Study.
This study was carried out among 160 nonpregnant women registered at the Outpatient Department of Gynaecology/Obstetrics of KIST Medical College Teaching Hospital, Imadol, Lalitpur, Nepal, from November 2014 to May 2015. The aim of the study was to assess the association of the risk factors with BV and analyze the type of bacteria associated with BV. Nugent's scoring method was used for diagnosis of BV in this study. The overall prevalence of BV was 24.4% among symptomatic patients. Douching was statistically related to BV (P = 0.015). Also, BV was significantly associated with consistency (P = 0.0001), odor (P = 0.02), an...
Source: International Journal of Microbiology - April 27, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Bacterial Profile, Antibacterial Resistance Pattern, and Associated Factors from Women Attending Postnatal Health Service at University of Gondar Teaching Hospital, Northwest Ethiopia.
Conclusion: The high prevalence of bacterial profile and isolation of multidrug-resistant bacteria pose a big threat to postnatal mothers and their children. Factors such as cesarean section, episiotomy for delivery, and premature rapture of membrane were predictors for bacterial infection. Therefore, there should be done a continuous surveillance as well as rational use of antibiotics and a longitudinal study using phenotypic and genotypic methods will be done. PMID: 29681942 [PubMed] (Source: International Journal of Microbiology)
Source: International Journal of Microbiology - April 24, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Morphological and Molecular Identification of the Causal Agent of Anthracnose Disease of Avocado in Kenya.
Authors: Kimaru SK, Monda E, Cheruiyot RC, Mbaka J, Alakonya A Abstract Anthracnose disease of avocado contributes to a huge loss of avocado fruits due to postharvest rot in Kenya. The causal agent of this disease has not been clear but presumed to be Colletotrichum gloeosporioides as reported in other regions where avocado is grown. The fungus mainly infects fruits causing symptoms such as small blackish spots, "pepper spots," and black spots with raised margin which coalesce as infection progresses. Due to economic losses associated with the disease and emerging information of other species of fungi as ...
Source: International Journal of Microbiology - April 24, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Effects of Bile Acids and Nisin on the Production of Enterotoxin by Clostridium perfringens in a Nutrient-Rich Medium.
Authors: Park M, Rafii F Abstract Clostridium perfringens is the second most common cause of bacterial foodborne illness in the United States, with nearly a million cases each year. C. perfringens enterotoxin (CPE), produced during sporulation, damages intestinal epithelial cells by pore formation, which results in watery diarrhea. The effects of low concentrations of nisin and bile acids on sporulation and toxin production were investigated in C. perfringens SM101, which carries an enterotoxin gene on the chromosome, in a nutrient-rich medium. Bile acids and nisin increased production of enterotoxin in cultures; b...
Source: International Journal of Microbiology - April 21, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research

Bacterial Community of the Rice Floodwater Using Cultivation-Independent Approaches.
In this study, bacterial communities in floodwaters of rice fields were monitored during the vegetative and reproductive stages of rice plant development using 16S amplicon sequencing. The study was conducted in the south of Brazil, during the crop years 2011/12 and 2012/13. Comparative analyses showed strong differences between the communities of floodwaters associated with the two developmental stages. During the vegetative stage, 1551 operational taxonomic units (OTUs) were detected, while less than half that number (603) were identified in the reproductive stage. The higher bacterial richness observed in floodwater col...
Source: International Journal of Microbiology - April 20, 2018 Category: Microbiology Tags: Int J Microbiol Source Type: research