Phenotypic and Genotypic Characterization of Extended Spectrum Beta-Lactamase-Producing Clinical Isolates of < em > Escherichia coli < /em > and < em > Klebsiella pneumoniae < /em > in Two Kenyan Facilities: A National Referral and a Level Five Hospital
CONCLUSIONS: The high proportion of ESBL-producing E. coli and K. pneumoniae isolates increases the utilization of last-resort antibiotics, jeopardizing antimicrobial chemotherapy. Furthermore, the antimicrobial resistance patterns exhibited towards extended-spectrum cephalosporins, beta-lactam/beta-lactamase inhibitor combinations, fluoroquinolones, and macrolides show the risk of co-resistance associated with ESBL-producing isolates responsible for MDR. Hence, there is a need for regular surveillance and implementation of infection prevention and control strategies and antimicrobial stewardship programs.PMID:38384586 | P...
Source: International Journal of Microbiology - February 22, 2024 Category: Microbiology Authors: Sylvia M Maveke Gabriel O Aboge Laetitia W Kanja Alfred O Mainga Naftaly Gachau Beatrice W Muchira Gervason A Moriasi Source Type: research

Metagenomic Characterization of Poultry Cloacal and Oropharyngeal Swabs in Kenya Reveals Bacterial Pathogens and Their Antimicrobial Resistance Genes
We report that Proteobacteria, Chlamydiae, and Firmicutes are the most dominant phyla present in both cloacal and oropharyngeal swabs of the six poultry species studied, indicating the colonization of the poultry gut by many pathogenic bacteria. Using KEGG and COG databases, some pathways related to metabolism, genetic information, and cellular processing were detected. We also report the abundance of antimicrobial resistance genes that confer resistance to β-lactamases, aminoglycosides, and tetracycline in most of the poultry analyzed, raising concern about the dangers associated with continuous and inappropriate use of ...
Source: International Journal of Microbiology - February 20, 2024 Category: Microbiology Authors: Philip M Panyako Sheila C Ommeh Stephen N Kuria Jacqueline K Lichoti Johns Musina Venugopal Nair Vish Nene Muhammad Munir Samuel O Oyola Source Type: research

Antibiotic Resistance Profile of < em > E. coli < /em > Isolates from Lettuce, Poultry Manure, Irrigation Water, and Soil in Kumasi, Ghana
Int J Microbiol. 2024 Jan 27;2024:6681311. doi: 10.1155/2024/6681311. eCollection 2024.ABSTRACTInputs such as irrigation water and poultry manure used in lettuce cultivation have been found to be associated with antibiotic-resistant pathogens. The study assessed the antibiotic resistance profile of Escherichia coli isolated from lettuce, poultry manure, irrigation water, and soil in Kumasi. One hundred and fifty-six samples of lettuce, irrigation water, soil, and manure were collected from three farms over a seven-week cultivation period (seedlings to harvest stage). E. coli were enumerated using standard methods. 98% of t...
Source: International Journal of Microbiology - February 5, 2024 Category: Microbiology Authors: Abigail Abena Anokyewaa Appau Linda Aurelia Ofori Source Type: research