International Journal of Microbiology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Detection of Pathogenic Serogroups and Virulence Genes in < em > Listeria monocytogenes < /em > Strains Isolated from Beef and Beef Products Retailed in Gauteng Province, South Africa, Using Phenotypic and Polymerase Chain Reaction (PCR)-Based Methods
Int J Microbiol. 2024 Mar 13;2024:8891963. doi: 10.1155/2024/8891963. eCollection 2024.ABSTRACTSouth Africa recently (2017-18) experienced the largest outbreak of human listeriosis in the world caused by L. monocytogenes following the consumption of "polony," a ready-to-eat meat product. Most (59%) cases originated from Gauteng province, South Africa. As a follow-up study to the outbreak, we used standard bacteriological and molecular methods to determine the prevalence of pathogenic and virulent serogroups of L. monocytogenes in various beef and beef products retailed in Gauteng province, South Africa. The overall prevale...
Source: International Journal of Microbiology - March 21, 2024 Category: Microbiology Authors: James Gana Nomakorinte Gcebe Rebone Moerane Yusuf B Ngoshe Khomotso Moabelo Abiodun A Adesiyun Source Type: research

Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples
Int J Microbiol. 2024 Mar 11;2024:4894004. doi: 10.1155/2024/4894004. eCollection 2024.ABSTRACTPCR and its variants (RT-PCR and qRT-PCR) are valuable and innovative molecular techniques for studying nucleic acids. qPCR has proven to be highly sensitive, efficient, and reproducible, generating reliable results that are easy to analyze. During the COVID-19 pandemic, qPCR became the gold standard technique for detecting the SARS-CoV-2 virus that allowed to confirm the infection event, and those asymptomatic ones, and thus save millions of lives. In-house multiplex qPCR tests were developed worldwide to detect different viral ...
Source: International Journal of Microbiology - March 19, 2024 Category: Microbiology Authors: Camilo Castellar-Mendoza Mar ía-Angélica Calderón-Peláez Jaime E Castellanos Myriam L Velandia-Romero Carolina Coronel-Ruiz Sigrid Camacho-Ortega Lilia J Bernal-Cepeda Lady L ópez-Ibarra Jhann A Arturo F élix G Delgado Hernando Gutierrez-Barbosa Son Source Type: research

< em > Mycobacterium tuberculosis < /em > Lineage Distribution Using Whole-Genome Sequencing and Bedaquiline, Clofazimine, and Linezolid Phenotypic Profiles among Rifampicin-Resistant Isolates from West Java, Indonesia
This study aimed to determine the association between the TB lineage distribution using whole-genome sequencing and bedaquiline, clofazimine, and linezolid phenotypic profile resistance among M. tuberculosisrifampicin-resistant isolates from West Java. M. tuberculosis isolates stock of the Department of Microbiology, Faculty of Medicine, Universitas Indonesia, was tested against bedaquiline, clofazimine, and linezolid using a mycobacteria growth indicator tube liquid culture. All isolates were tested for M. tuberculosis and rifampicin resistance using Xpert MTB/RIF. The DNA genome of M. tuberculosis was freshly extracted f...
Source: International Journal of Microbiology - March 12, 2024 Category: Microbiology Authors: Andriansjah Rukmana Cynthia Gozali Linda Erlina Source Type: research