Generation of human antibodies targeting human platelet antigen (HPA)-1a
This study aimed to isolate HPA-1a-specific B-cells from an HPA-1a-alloimmunized pregnant woman. Using fluorescently labeled HPA-1a-positive platelets, single B-cells were sorted and cultured for 10 days to stimulate antibody production. Subsequently, supernatants were tested for the presence of antibodies by enzyme-linked immunosorbent assay and their reactivity towards HPA-1a-positive platelets. Amplification and sequencing of variable regions allowed the generation of monoclonal antibodies using a HEK-Freestyle-based expression system.RESULTS: Three platelet-specific B-cells were obtained and cloned of which two were sp...
Source: Transfusion - February 24, 2024 Category: Hematology Authors: Janita J Oosterhoff Federica Linty Remco Visser Thijs de Vos Suzanne Hofstede-van Egmond Miranda van de Weerd Leendert Porcelijn Masja de Haas Ellen van der Schoot Gestur Vidarsson Source Type: research
Blood donation and migraine relief: A national population cohort study in Denmark
DISCUSSION: Our findings do not support the hypothesis that blood donation serves as a viable treatment strategy among migraine patients. Future prospective investigations may help to elucidate the underlying biological mechanisms by which blood donation may influence migraine pathology.PMID:38400775 | DOI:10.1111/trf.17770 (Source: Transfusion)
Source: Transfusion - February 24, 2024 Category: Hematology Authors: Olafur B Davidsson Klaus Rostgaard Mona A Chalmer Lisette J A Kogelman Bitten Aagaard Thorsten Brodersen Mie Topholm Bruun Christina Mikkelsen Susan Mikkelsen Mette Nyegaard Ole Birger Pedersen Henrik Ullum Erik S ørensen Sisse Rye Ostrowski Christian Er Source Type: research
Classification of posttransfusion adverse events using a publicly available artificial intelligence system
DISCUSSION: A generative AI system may have a role in helping healthcare providers to consider transfusion reaction categories that might be missed, but caution is advised in applying the AI's output to transfusion reaction classification at present.PMID:38400777 | DOI:10.1111/trf.17702 (Source: Transfusion)
Source: Transfusion - February 24, 2024 Category: Hematology Authors: Mark K Fung James P AuBuchon Laura D Stephens Source Type: research
Functional effects of an African glucose-6-phosphate dehydrogenase (G6PD) polymorphism (Val68Met) on red blood cell hemolytic propensity and post-transfusion recovery
DISCUSSION: This novel mouse model of the common G6PD A- variant has impaired antioxidant capacity like humans and low G6PD activity may reduce survival of transfused RBCs when irradiation is performed.PMID:38400625 | DOI:10.1111/trf.17756 (Source: Transfusion)
Source: Transfusion - February 24, 2024 Category: Hematology Authors: Ling Wang Elizabeth R Rochon Sebastien Gingras Benjamin E Zuchelkowski Derek J Sinchar Elimira Alipour Julie A Reisz Minying Yang Grier P Page Tamir Kanias Darrell J Triulzi Janet S Lee Daniel B Kim-Shapiro Angelo D'Alessandro Mark T Gladwin Source Type: research
Autoantibody profiles in intravenous immunoglobulin preparations: A possible cause of mistaken autoimmunity diagnosis
DISCUSSION: To the best of our knowledge, this is the first study that analyzed multiple autoantibody profiles in both pooled plasma and IVIg products and that further evaluated their potential influences on diagnosis of autoimmunity. Clinicians should keep in mind that IVIgs contain several autoantibodies and that their infusion can produce false-positive serology results. To establish an accurate diagnosis, serological tests must be carefully interpreted and clinical symptoms should be more purposefully considered if patients are receiving IVIg therapy.PMID:38400628 | DOI:10.1111/trf.17766 (Source: Transfusion)
Source: Transfusion - February 24, 2024 Category: Hematology Authors: Tatsuki Miyamoto Yuki Fukunaga Atsushi Ogasawara Ai Munakata Katsushi Murai Source Type: research
The T ägerwilen II report: Recommendations from the NATO Prehospital Care Improvement Initiative Task Force
CONCLUSION: The recommendations on these four themes reflect the most significant priorities in improving prehospital care, and must be incorporated in the on-going revision of NATO doctrine.PMID:38400632 | DOI:10.1111/trf.17760 (Source: Transfusion)
Source: Transfusion - February 24, 2024 Category: Hematology Authors: Christian Medby Colleen Forestier Benjamin Ingram Duncan Parkhouse Michael Alvarez-Brueckmann Alexander Faas Source Type: research
Generation of human antibodies targeting human platelet antigen (HPA)-1a
This study aimed to isolate HPA-1a-specific B-cells from an HPA-1a-alloimmunized pregnant woman. Using fluorescently labeled HPA-1a-positive platelets, single B-cells were sorted and cultured for 10 days to stimulate antibody production. Subsequently, supernatants were tested for the presence of antibodies by enzyme-linked immunosorbent assay and their reactivity towards HPA-1a-positive platelets. Amplification and sequencing of variable regions allowed the generation of monoclonal antibodies using a HEK-Freestyle-based expression system.RESULTS: Three platelet-specific B-cells were obtained and cloned of which two were sp...
Source: Transfusion - February 24, 2024 Category: Hematology Authors: Janita J Oosterhoff Federica Linty Remco Visser Thijs de Vos Suzanne Hofstede-van Egmond Miranda van de Weerd Leendert Porcelijn Masja de Haas Ellen van der Schoot Gestur Vidarsson Source Type: research
Blood donation and migraine relief: A national population cohort study in Denmark
DISCUSSION: Our findings do not support the hypothesis that blood donation serves as a viable treatment strategy among migraine patients. Future prospective investigations may help to elucidate the underlying biological mechanisms by which blood donation may influence migraine pathology.PMID:38400775 | DOI:10.1111/trf.17770 (Source: Transfusion)
Source: Transfusion - February 24, 2024 Category: Hematology Authors: Olafur B Davidsson Klaus Rostgaard Mona A Chalmer Lisette J A Kogelman Bitten Aagaard Thorsten Brodersen Mie Topholm Bruun Christina Mikkelsen Susan Mikkelsen Mette Nyegaard Ole Birger Pedersen Henrik Ullum Erik S ørensen Sisse Rye Ostrowski Christian Er Source Type: research
Classification of posttransfusion adverse events using a publicly available artificial intelligence system
DISCUSSION: A generative AI system may have a role in helping healthcare providers to consider transfusion reaction categories that might be missed, but caution is advised in applying the AI's output to transfusion reaction classification at present.PMID:38400777 | DOI:10.1111/trf.17702 (Source: Transfusion)
Source: Transfusion - February 24, 2024 Category: Hematology Authors: Mark K Fung James P AuBuchon Laura D Stephens Source Type: research
Other bacterial agents (4th section)
Transfusion. 2024 Feb;64 Suppl 1:S243-S270. doi: 10.1111/trf.17695.NO ABSTRACTPMID:38394037 | DOI:10.1111/trf.17695 (Source: Transfusion)
Source: Transfusion - February 23, 2024 Category: Hematology Source Type: research
Viral agents (2nd section)
Transfusion. 2024 Feb;64 Suppl 1:S19-S207. doi: 10.1111/trf.17630.NO ABSTRACTPMID:38394038 | DOI:10.1111/trf.17630 (Source: Transfusion)
Source: Transfusion - February 23, 2024 Category: Hematology Source Type: research
Prion agents (1st section)
Transfusion. 2024 Feb;64 Suppl 1:S4-S18. doi: 10.1111/trf.17627.NO ABSTRACTPMID:38394039 | DOI:10.1111/trf.17627 (Source: Transfusion)
Source: Transfusion - February 23, 2024 Category: Hematology Source Type: research
Bacterial agents (3rd section)
Transfusion. 2024 Feb;64 Suppl 1:S208-S242. doi: 10.1111/trf.17693.NO ABSTRACTPMID:38394040 | DOI:10.1111/trf.17693 (Source: Transfusion)
Source: Transfusion - February 23, 2024 Category: Hematology Source Type: research
Infectious disease agents and their potential threat to transfusion safety (an update to the 2009 Transfusion supplement)
Transfusion. 2024 Feb;64 Suppl 1:S1-S3. doi: 10.1111/trf.17626.NO ABSTRACTPMID:38394041 | DOI:10.1111/trf.17626 (Source: Transfusion)
Source: Transfusion - February 23, 2024 Category: Hematology Authors: L M Katz Roger Y Dodd Paula Saa J B Gorlin K Palmer F B Hollinger S L Stramer Source Type: research
Protozoan agents and nematode agents (5th section)
Transfusion. 2024 Feb;64 Suppl 1:S271-S287. doi: 10.1111/trf.17694.NO ABSTRACTPMID:38394043 | DOI:10.1111/trf.17694 (Source: Transfusion)
Source: Transfusion - February 23, 2024 Category: Hematology Source Type: research
