Molecular characterization of diarrheagenic Escherichia coli from an outpatient pediatric population with diarrhea attended in two hospitals from Buenos Aires, Argentina
The objective of the study was to characterize the isolates of diarrheagenic E.coli from an outpatient pediatric population with diarrhea attended in two public hospitals from Buenos Aires, Argentina. Diarrheagenic E.coli pathotypes were investigated by amplifying characteristic virulence gene fragments: intimin (eae), heat-labile toxin (lt), heat-stable toxins (stp, sth), invasion plasmid antigen H (ipaH), transcriptional activator R (aggR) and Shiga toxins (stx1, stx2). Molecular subtyping of isolates was performed using PFGE (XbaI). Diarrheagenic E.coli was detected in 14% (84/601) of cases. The EAEC pathotype was preva...
Source: Revista Argentina de Microbiologia - July 27, 2023 Category: Microbiology Authors: Nora Beatriz Molina Sebasti án Oderiz Marisa Ana L ópez Juan Ángel Basualdo M ónica Delfina Sparo Source Type: research
Epidemiological study and serotyping by multiple PCR of Listeria monocytogenes isolated from food matrices in Argentina
The objective of this study was to detect and serotype L.monocytogenes isolated from different food matrices from processing plants in Argentina. In the period 2016-2021, 1832 samples (meat, ready-to-eat foods, ice cream, dairy foods, and frozen vegetables) were analyzed, of which 226 (12.34%) isolates compatible with L.monocytogenes were detected. At the same time, environmental and surface samplings were performed in processing plants for ready-to-eat foods, sausages and dairy products, where environmental contamination with L.monocytogenes was detected in numerous critical points of the process, yielding a positivity ra...
Source: Revista Argentina de Microbiologia - July 21, 2023 Category: Microbiology Authors: Yamila Figueroa Jimena Gentiluomo Agustina Grisaro Mariana Buffoni Nadia Zipenco Adriana Sucari Paula Buonfiglio Magdalena Costa Source Type: research
Optimization in the detection of bacteremia: Integrated use of FilmArray (BCID2) and MALDI-TOF
Rev Argent Microbiol. 2023 Jul 18:S0325-7541(23)00052-4. doi: 10.1016/j.ram.2023.06.001. Online ahead of print.ABSTRACTThe usefulness of the combined use of MALDI-TOF MS from a subculture with 3-5h of incubation and the BCID2 panel (FilmArray) for the identification of microorganisms from positive blood cultures and its importance in the adjustment of antimicrobial therapy was analyzed. Overall identification with BCID2 was 90.4% (142/157) and with Maldi-TOF MS 83.4% (131/157) (p=0.0858); in 23 polymicrobial episodes (47 strains), the BCID2 panel identified 45 (95.7%) and MALDI-TOF MS 24 (51.1%) (p<0.0000). BCID2 detect...
Source: Revista Argentina de Microbiologia - July 20, 2023 Category: Microbiology Authors: Natalia A Carrion Rodolfo E Quiroz Camila Asenzo Manuela Gamarra Antonio E Flores Maria Garcia Rolando N Soloaga Julia Grigioni Maria Mastroianni Alejandra Margari Facundo Molina Adriana N Procopio Vanesa Reijtman Maria S Ratti Victor Mamani Myriam Vazque Source Type: research
Differential diagnosis of human Entamoeba infections: Morphological and molecular characterization of new isolates in Argentina
This study aimed to determine the prevalence of Entamoeba species in two populations from Argentina, make a differential diagnosis by PCR and characterize Entamoeba isolates at the SSU rRNA gene. A total of 493 serial fecal samples were obtained from individuals in the provinces of Buenos Aires (n=210) and Misiones (n=283). Samples were examined by conventional methods (formalin-ethyl acetate and Willis flotation) and specific PCRs to differentiate Entamoeba species. Entamoeba isolates were characterized by sequencing a fragment of the SSU rRNA gene. The overall prevalence of Entamoeba infection was 12.4%, being more preva...
Source: Revista Argentina de Microbiologia - July 5, 2023 Category: Microbiology Authors: Andrea Servi án Mar ía Lorena Zonta Graciela T Navone Source Type: research
