Growth modeling to control (in vitro) Fusarium verticillioides and Rhizopus stolonifer with thymol and carvacrol.
Authors: Ochoa-Velasco CE, Navarro-Cruz AR, Vera-López O, Palou E, Avila-Sosa R Abstract The aim of this study was to evaluate the antifungal activity (in vitro) of thymol and carvacrol alone or in mixtures against Fusarium verticillioides and Rhizopus stolonifer, and to obtain primary growth models. Minimal inhibitory concentration (MIC) was evaluated with fungal radial growth with thymol or carvacrol concentrations (0-1600mg/l). Mixtures were evaluated using concentrations below MIC values. Radial growth curves were described by the modified Gompertz equation. MIC values of carvacrol were 200mg/l for both ...
Source: Revista Argentina de Microbiologia - September 29, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Combined effect of bacteriocin produced by Lactobacillus plantarum ST8SH and vancomycin, propolis or EDTA for controlling biofilm development by Listeria monocytogenes.
Authors: Todorov SD, de Paula OAL, Camargo AC, Lopes DA, Nero LA Abstract The Listeria monocytogenes strains selected in the present study exhibited similar behavior in biofilm formation, independently of the tested conditions (bacteriocin from L. plantarum ST8SH, vancomycin, propolis (a natural antimicrobial product) and EDTA (chelating agent)), individual or in associations. The individual application of vancomycin had better inhibitory activity than that of propolis and EDTA; however, the association of the previously mentioned antimicrobial agents with bacteriocins resulted in better performance. However, when ...
Source: Revista Argentina de Microbiologia - September 29, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Antimicrobial activity of different sodium and potassium salts of carboxylic acid against some common foodborne pathogens and spoilage-associated bacteria.
The objective of the present study was to evaluate the antimicrobial activity of different sodium and potassium salts of aliphatic and aromatic carboxylic acid on the growth of common food spoilage and pathogenic microorganisms. Growth curves were determined for Leuconostoc mesenteroides, Lactobacillus plantarum, Enterococcus faecalis, Candida albicans, Pseudomonas aeruginosa, Salmonella Enteritidis, and Listeria monocytogenes in contact with different concentrations of carboxylic acid salts. The inhibitory effect of both aliphatic and aromatic carboxylic acid salts, in accordance with concentration levels, was 100>50&g...
Source: Revista Argentina de Microbiologia - September 29, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Nitrous oxide emission, nitrification, denitrification and nitrogen mineralization during rice growing season in 2 soils from Uruguay].
Authors: Illarze G, Del Pino A, Riccetto S, Irisarri P Abstract Microbial processes such as mineralization, nitrification and denitrification regulate nitrogen dynamics in the soil. The last two processes may produce nitrous oxide (N2O). In this work N2O fluxes were quantified at four moments of the rice cycle, sowing, tillering, panicle initiation and maturity, in two sites that differed mainly in their soil organic matter (OM) content, Salto (higher OM) and Treinta y Tres. Potential net N mineralization, ammonium oxidation and denitrification as well as the most probable numbers (MPN) of ammonia oxidizers and den...
Source: Revista Argentina de Microbiologia - September 29, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates.
CONCLUSION: All azole compounds tested in the study showed inhibitory activity against MDR M. tuberculosis clinical isolates. PMID: 28919150 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - September 19, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Uptake of cholesterol by Tetrahymena thermophila is mainly due to phagocytosis.
Authors: Elguero ME, Sanchez Granel ML, Montes MG, Cid NG, Favale NO, Nudel CB, Nusblat AD PMID: 28916309 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - September 18, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Passive immunity to control Bovine coronavirus diarrhea in a dairy herd in Argentina.
Authors: Bok M, Alassia M, Frank F, Vega CG, Wigdorovitz A, Parreño V Abstract Bovine coronavirus (BCoV) is a viral enteric pathogen associated with calf diarrhea worldwide being, in Argentina, mostly detected in dairy husbandry systems. The aim of the present work was to study if maternal IgG1 antibodies (Abs) to BCoV acquired by colostrum intake modulate the development of BCoV infection in calves reared in a dairy farm in Argentina. Thirty Holstein calves were monitored during their first 60 days of age. Animals were classified into two groups depending on their initial BCoV IgG1 Ab titers. The "fail...
Source: Revista Argentina de Microbiologia - September 14, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Combination of phosphorus solubilizing and mobilizing fungi with phosphate rocks and volcanic materials to promote plant growth of lettuce (Lactuca sativa L.)].
Authors: Velázquez MS, Cabello MN, Elíades LA, Russo ML, Allegrucci N, Schalamuk S Abstract Arbuscular mycorrhizal fungi (AMF) increase the uptake of soluble phosphates, while phosphorus solubilizing fungi (S) promote solubilization of insoluble phosphates complexes, favoring plant nutrition. Another alternative to maintaining crop productivity is to combine minerals and rocks that provide nutrients and other desirable properties. The aim of this work was to combine AMF and S with pyroclastic materials (ashes and pumices) from Puyehue volcano and phosphate rocks (PR) from Rio Chico Group (Chubut) - to...
Source: Revista Argentina de Microbiologia - September 14, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Dicistroviridae: A new viral purification technique.
Authors: Susevich ML, Metz GE, Marti GA, Echeverría MG Abstract The family Dicistroviridae comprises three genera and about twenty species of RNA virus, most of them with health or agricultural importance. The Triatoma virus (TrV) is the only entomopathogenic virus identified in triatomine bugs up to the present. TrV replicates within the intestinal epithelial cells, causing high mortality rate and delayed development of the molt of these bugs. TrV has been proposed as a biological control agent for vectors of Chagas disease. Viral particles were purified from feces of 1, 5 and 10 insects from an experimenta...
Source: Revista Argentina de Microbiologia - September 12, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Characterization of Actinobacillus pleuropneumoniae field strains antigenically related to the 3-6-8-15 group from diseased pigs in Japan and Argentina.
The objectives of this study were to determine the serovar of a collection of Actinobacillus pleuropneumoniae strains within the 3-6-8-15 cross-reacting group and to analyze their phenotypic and genetic properties. Based on the serological tests, forty-seven field strains of Actinobacillus pleuropneumoniae isolated from lungs with pleuropneumonia lesions in Japan and Argentina were found to be serovars belonging to the 3-6-8-15 cross-reacting group. By using a capsule loci-based PCR, twenty-nine (96.7%) and one (3.3%) from Japan were identified as serovars 15 and 8, respectively, whereas seventeen (100%) from Argentina wer...
Source: Revista Argentina de Microbiologia - September 11, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Assessment of growth of Aspergillus spp. from agricultural soils in the presence of glyphosate.
This study evaluated the growth performance of Aspergillus section Flavi and Aspergillus niger aggregate strains on Czapek Dox media supplied with a commercial glyphosate formulation as sole source of carbon (CZC), phosphorus (CZP) or nitrogen (CZN). Six Aspergillus spp. strains were evaluated. Each medium was stab-inoculated with fungal spores from 7-day old cultures. Two measures of colony radii were taken daily. All of the Aspergillus section Flavi strains showed a significant increase (from 24 to 44%) in growth rate on the CZN medium, as compared to controls. The A. niger aggregate strains exhibited the same behavioral...
Source: Revista Argentina de Microbiologia - September 9, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Azotobacter chroococcum as a potentially useful bacterial biofertilizer for cotton (Gossypium hirsutum): Effect in reducing N fertilization.
Authors: Romero-Perdomo F, Abril J, Camelo M, Moreno-Galván A, Pastrana I, Rojas-Tapias D, Bonilla R Abstract The aim of this research was to evaluate whether the application of two plant growth-promoting (rhizo)bacteria might reduce nitrogen fertilization doses in cotton. We used strains Azotobacter chroococcum AC1 and AC10 for their proven ability to promote seed germination and cotton growth. These microorganisms were characterized by their plant growth-promoting activities. Then, we conducted a glasshouse study to evaluate the plant growth promoting ability of these strains with reduced doses of urea fer...
Source: Revista Argentina de Microbiologia - September 4, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Burkholderia species associated with legumes of Chiapas, Mexico, exhibit stress tolerance and growth in aromatic compounds.
In this study, 15 bacterial strains were isolated from the nodules of the following legumes: Indigofera thibaudiana, Mimosa diplotricha, Mimosa albida, Mimosa pigra, and Mimosa pudica, collected in 9 areas of Chiapas, Mexico. The strains were grouped into four profiles of genomic fingerprints through BOX-PCR and identified based on their morphology, API 20NE biochemical tests, sequencing of the 16S rRNA, nifH and nodC genes as bacteria of the Burkholderia genus, genetically related to Burkholderia phenoliruptrix, Burkholderia phymatum, Burkholderia sabiae, and Burkholderia tuberum. The Burkholderia strains were grown under...
Source: Revista Argentina de Microbiologia - September 4, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Microbiological assessment of honey in M éxico.
Microbiological assessment of honey in México. Rev Argent Microbiol. 2017 Aug 16;: Authors: Vázquez-Quiñones CR, Moreno-Terrazas R, Natividad-Bonifacio I, Quiñones-Ramírez EI, Vázquez-Salinas C Abstract Honey is a product used as a natural sweetener and in several regions of Mexico and other countries it is also used as a therapeutic agent. Microbiological contamination of honey can occur during its extraction and handling. Due to the use and consumption of honey we highlighted here the importance of the assessment of its microbiological quality. One thousand n...
Source: Revista Argentina de Microbiologia - August 23, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Spatial and seasonal characterization of the drinking water from various sources in a peri-urban town of Salta].
Authors: Rodriguez-Alvarez MS, Moraña LB, Salusso MM, Seghezzo L Abstract Drinking water monitoring plans are important to characterize both treated and untreated water used for drinking purposes. Access to drinking water increased in recent years as a response to the Millennium Development Goals set for 2015. The new Sustainable Development Goals aim to ensure universal access to safe drinking water by 2030. Within the framework of these global goals, it is crucial to monitor local drinking water systems. In this paper, treated and untreated water from different sources currently consumed in a specific town...
Source: Revista Argentina de Microbiologia - August 23, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Effect of mixed edaphic bacterial inoculants in the early development of improved cocoa cultivars (Theobroma cacao L.) in a traditional agroforestry system of Oaxaca, Mexico].
Authors: Hipólito-Romero E, Carcaño-Montiel MG, Ramos-Prado JM, Vázquez-Cabañas EA, López-Reyes L, Ricaño-Rodríguez J Abstract Cocoa plant (Theobroma cacao L.) is native from South America and it represents one of the most significant "bio-cultural" resources of Mesoamerica, since it is a region where it was domesticated and had a relevance as ritual drink and as currency in many pre-hispanic cultures until the arrival of the Spaniards who spread its use worldwide, and became it one of the most consumed commodity goods. Through this research, an alternative...
Source: Revista Argentina de Microbiologia - August 6, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Streptococcus uberis: In vitro biofilm production in response to carbohydrates and skim milk.
Authors: Dieser SA, Fessia AS, Ferrari MP, Raspanti CG, Odierno LM Abstract Streptococcus uberis has become one of the most important environmental pathogens associated with clinical and subclinical bovine mastitis. Biofilm confers to bacteria more resistance to physical and chemical agents as well as to different mechanisms of the innate immune system. The aim of this work was to evaluate the ability of in vitro biofilm production in 32 S. uberis isolates from bovine mastitis and identified by biochemical tests and subsequently confirmed by the amplification of the pauA gene. The isolates were cultivated in TMP br...
Source: Revista Argentina de Microbiologia - August 6, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Microbial inoculants: Pieces of a puzzle that still needs to be assembled].
Authors: Creus CM PMID: 28732672 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - July 24, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Bifidobacterium scardovii isolated from 2 consecutive urine samples].
Authors: Guevara Nuñez D, De Paulis AN, Bertona E, Gutiérrez MÁ, Vay CA, Suárez JP, Predari SC Abstract Bifidobacterium scardovii species consists of facultative anaerobic gram-positive rods whose growth is stimulated by CO2 and anaerobiosis. Exceptionally it has been associated with infections in humans. An elderly male patient with a urinary tract infection due to B. scardovii and Enterococcus faecalis is presented here; both microorganisms were isolated from two consecutive urine samples. The bacillus did not grow on standard media, but on chocolate agar incubated in CO2 and on supple...
Source: Revista Argentina de Microbiologia - July 24, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Candidemia in Pediatrics: Species distribution and antifungal susceptibility].
Authors: Guzzetti LB, Vescina CM, Gil MF, Gatti BM Abstract Serious infections caused by Candida yeasts are frequent in the hospital population. Due to differences in species distribution and antifungal susceptibility testing depending on the geographic area and the type of patient, it is important to study the epidemiology of each institution. For this purpose, we conducted a retrospective, descriptive study on the occurrence of candidemia in the Children's Hospital "Superiora Sor María Ludovica" of the city of La Plata. In a 6-year period (2010-2015), 177 candidemia episodes were recorded. The pr...
Source: Revista Argentina de Microbiologia - July 24, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Epidemiological and molecular characteristics of Chlamydia psittaci from 8 human cases of psittacosis and 4 related birds in Argentina.
This report contributes to our increasing knowledge of the epidemiological and molecular characteristics of C. psittaci to conduct effective surveillance of its zoonotic infections. PMID: 28734713 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - July 24, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Inactivation of Alicyclobacillus acidoterrestris ATCC 49025 spores in apple juice by pulsed light. Influence of initial contamination and required reduction levels.
Authors: Ferrario MI, Guerrero SN Abstract The purpose of this study was to analyze the response of different initial contamination levels of Alicyclobacillus acidoterrestris ATCC 49025 spores in apple juice as affected by pulsed light treatment (PL, batch mode, xenon lamp, 3pulses/s, 0-71.6J/cm(2)). Biphasic and Weibull frequency distribution models were used to characterize the relationship between inoculum size and treatment time with the reductions achieved after PL exposure. Additionally, a second order polynomial model was computed to relate required PL processing time to inoculum size and requested log reduc...
Source: Revista Argentina de Microbiologia - July 23, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Development of a liquid fermentation system and encystment for a nitrogen-fixing bacterium strain having biofertilizer potential].
The objective of this research was to develop a suitable liquid fermentation system and encystment for the multiplication of Azotobacter chroococcum AC1 strain, a bacterium employed in a biofertilizer formulation produced at present by CARPOICA, Colombia. Sequential statistical designs were used to determine the conditions in the fermentation system. The interaction between agitation, aeration and pH was evaluated on the viable biomass (CFU/ml) of AC1. In addition, the encystment ability of the strain was evaluated using two encystment agents and the potential plant growth-promoting rhizobacteria (PGPR) activity was assess...
Source: Revista Argentina de Microbiologia - July 21, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Health care importance of Treponema pallidum, Chagas' disease and Human immunodeficiency virus 1 among Amerindians of Argentina: an observational study.
The objective of this study was to estimate the prevalence of Treponema pallidum, Trypanosoma cruzi and Human immunodeficiency virus 1 (HIV-1) in five Amerindian populations of Argentina. A retrospective study was conducted among 857 Amerindian populations (112 Kollas, 298 Mbyá-guaraníes, 79 Sagua Huarpes, 368 Wichis) from 2007 to 2010. Screening and confirmation of T. pallidum, T. cruzi and HIV-1 were performed. T. pallidum and T. cruzi infections were detected in all communities with an overall prevalence rate of 4.2% and 16.8%, respectively. Although HIV was not detected, syphilis and Chagas' disease repre...
Source: Revista Argentina de Microbiologia - July 19, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Genetic diversity of thermotolerant Campylobacter spp. isolates from different stages of the poultry meat supply chain in Argentina.
The objective of this study was to investigate a clonal relationship among thermotolerant Campylobacter spp. isolates from different stages of the poultry meat supply chain in Argentina. A total of 128 thermotolerant Campylobacter spp. (89 C. jejuni and 39 C. coli) isolates from six poultry meat chains were examined. These isolates were from: a) hens from breeder flocks, b) chickens on the farm (at ages 1 wk and 5 wk), c) chicken carcasses in the slaughterhouse, and d) chicken carcasses in the retail market. Chickens sampled along each food chain were from the same batch. Campylobacter spp. isolates were analyzed using pul...
Source: Revista Argentina de Microbiologia - July 19, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Recombinant Bovine herpervirus 5 expressing enhanced green fluorescent protein.
Authors: Silvestro CA, Bratanich AC PMID: 28711349 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - July 17, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Prevalence of Trypanosoma cruzi infection in dogs and small mammals in Nuevo Le ón, Mexico.
In conclusion, although the prevalence of T. cruzi infection in small mammals was lower in Nuevo León than in other states of Mexico, our results provide new locality records, including striped skunks, opossums (Didelphis marsupialis) and dogs, and extend the recorded area to woodrats (Neotoma micropus). PMID: 28705488 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - July 16, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Bacillus thuringiensis: New applications for an old acquaintance?]
Authors: Sauka DH PMID: 28578760 [PubMed - in process] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - June 7, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Microbiological quality of Argentinian paprika.
Authors: Melo González MG, Romero SM, Arjona M, Larumbe AG, Vaamonde G Abstract The aim of this study was to evaluate the microbiological quality of paprika produced in Catamarca, Argentina. Microbiological analyses were carried out for the enumeration of total aerobic mesophilic bacteria, coliforms, yeasts and molds, and the detection of Salmonella in samples obtained from different local producers during three consecutive years. The mycobiota was identified paying special attention to the mycotoxigenic molds. Standard plate counts of aerobic mesophilic bacteria ranged from 2.7×10(5) to 3.7×10(7...
Source: Revista Argentina de Microbiologia - June 4, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Hygienic-sanitary quality in abattoirs from Tucuman province, Argentina. Detection, isolation and characterization of Shiga toxin-producing Escherichia coli].
Authors: Pérez Terrazzino GB, Condorí MS, López Campo A, Vega S, Carbonari C, Chinen I, Rivas M, de Castillo MC, Jure MA Abstract Cattle are the main reservoir of Shiga toxin-producing Escherichia coli (STEC), and the strategies to prevent the transmission of these microorganisms are concentrated in the slaughtering plant. The aim of this study was to evaluate the hygienic-sanitary quality and the frequency of detection of STEC in beef carcasses in abattoirs from Tucuman province. Two hundred and seventy four beef carcass sponges were processed; the count of generic E. coli was marginal in 9 (3...
Source: Revista Argentina de Microbiologia - June 4, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Diversity and distribution of lepidopteran-specific toxin genes in Bacillus thuringiensis strains from Argentina.
Authors: Sauka DH, Benintende GB Abstract A total of 268 Bacillus thuringiensis strains obtained from different sources of Argentina were analyzed to determine the diversity and distribution of the cry1, cry2, cry8, cry9 and vip3A genes encoding for lepidopteran-specific insecticidal proteins. Twin strains were excluded. Ten different profiles were detected among the 80 selected B. thuringiensis strains. Two of these profiles (cry1Aa, cry1Ac, cry1Ia, cry2Aa, cry2Ab and vip3Aa (35/80), and cry1Aa, cry1Ab, cry1Ac, cry1Ia, cry2Aa, cry2Ab and vip3Aa (25/80)) pooled 75% of the strains. The existence of this low diversit...
Source: Revista Argentina de Microbiologia - June 4, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Multiplex PCR assay for genotyping of Mycobacterium tuberculosis in Lima, Peru.
Authors: Tello CA, Borja N, Gavilan RG, Garcia-de-la-Guarda R PMID: 28554706 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - June 1, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Squamocin, an annonaceous acetogenin, enhances naphthalene degradation mediated by Bacillus atrophaeus CN4.
Authors: Parellada EA, Igarza M, Isacc P, Bardón A, Ferrero M, Ameta KL, Neske A Abstract Squamocin belongs to a group of compounds called annonaceous acetogenins. They are secondary products of Annonaceae metabolism and can be isolated from Annona cherimolia seeds. This paper deals with the stimulation of biofilm formation of Bacillus atrophaeus CN4 by employing low squamocin concentrations to increase naphthalene degradation. Bacillus atrophaeus CN4, isolated from contaminated soil, has the ability to degrade naphthalene as the only source of carbon and energy. In the absence of additional carbon sources, ...
Source: Revista Argentina de Microbiologia - June 1, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Protothecosis in a patient with T cell lymphocytic leukemia.
We report a case of persisting subcutaneous protothecosis in a patient with T-cell large granular lymphocyte leukemia, who also presented a history of disseminated histoplasmosis. PMID: 28554708 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - June 1, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Biodiversity of species of Aspergillus section Fumigati in semi-desert soils in Argentina.
Authors: Giusiano GE, Piontelli E, Fernández MS, Mangiaterra ML, Cattana ME, Kocsubé S, Varga J Abstract The distribution of Aspergillus species in soil has been widely studied all over the world. The aim of this study was the phenotypic and genotypic characterization of species Aspergillus belonging to section Fumigati present in soils from two Argentinian semi-desert areas having different geological conditions. Altogether, 23 isolates belonging to Aspergillus section Fumigati were recovered and identified using a polyphasic approach including phenotypic and molecular identifications. Aspergillus fu...
Source: Revista Argentina de Microbiologia - May 31, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Indirect ELISA (iELISA) for routine detection of antibodies against Minute Virus of Mice (MVM) in mice colonies.
In this study we developed an indirect ELISA to detect antibodies against Minute Virus of Mice (MVM) using an antigen produced from BHK-21 cells infected with a prototype strain of the virus. The optimal antigen concentration and serum dilutions were established. In order to analyze variability in the laboratory, reproducibility and repeatability within and between plates were determined. Then, a panel of 460 sera from conventional facilities and previously classified as positive or negative by the indirect fluorescent antibody assay was analyzed. The cutoff value was determined by a receiver operating characteristic (ROC)...
Source: Revista Argentina de Microbiologia - May 31, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Anaerobiospirillum succiniciproducens and Desulfovibrio desulfuricans in 2 cases of insidious bacteremia].
Authors: Predari SC, de Paulis AN, Bertona E, Guevara Núñez D, Suárez JP, Castello L Abstract Two cases of insidious bacteremia by uncommon curve and spiral-shaped, motile anaerobic gram-negative rods are presented. Both of them were of an unclear origin and occurred in immunosuppressed patients with simultaneous diseases. The key tests for the identification of Anaerobiospirillum were its micromorphology, a strictly anaerobic condition, negative catalase activity, the special-potency disk profile, glucose fermentation, and β-NAG production. Desulfovibrio species was identified by all the ...
Source: Revista Argentina de Microbiologia - May 18, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Antimicrobial susceptibility of Helicobacter pylori strains isolated from gastric lesions].
Authors: Pereyra LDV, Gorordo Ipiña RC, Berruezo FA, Amieva CA, García ME, Bottiglieri MT Abstract Antimicrobial susceptibility was evaluated by two diffusion methods: E-test strips to determine minimum inhibitory concentration (MIC) and disk diffusion for amoxicillin, clarithromycin, metronidazole and ciprofloxacin in 30 Helicobacter pylori strains isolated from gastric biopsies. No strains were resistant to amoxicillin, 17% (5/30) were resistant to clarithromycin, 20% (6/30) ciprofloxacin by both methods, and 37% (11/30) to metronidazole by the E-test. Although the number of strains studied was redu...
Source: Revista Argentina de Microbiologia - May 18, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Presence of Acanthamoeba spp. in watering trough in La Pampa province, Argentina].
In this study, a survey was carried out to evaluate the presence of Acanthamoeba strains inwatering trough sample in La Pampa province, Argentina. Sample were inoculated onto non-nutrient agar plates and were checked for the presence of Acanthamoeba. Polymerase chain reaction was performed with genus-specific primers JDP1/JDP2, followed by direct sequencing of the polymerase chain reaction product for molecular identification. Sequencing results revealed the presence of T4, T5 and T15 genotypes within the studied samples. Sequencing revealed presence of T4, T5 and T15 in the samples studied genotypes, the most frequent T4....
Source: Revista Argentina de Microbiologia - May 14, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Selection of Bacillus thuringiensis strains toxic to cotton boll weevil (Anthonomus grandis, Coleoptera: Curculionidae) larvae.
Authors: Pérez MP, Sauka DH, Onco MI, Berretta MF, Benintende GB Abstract Preliminary bioassays with whole cultures (WC) of 124 Bacillus thuringiensis strains were performed with neonate larvae of Anthonomus grandis, a major cotton pest in Argentina and other regions of the Americas. Three exotic and four native strains were selected for causing more than 50% mortality. All of them were β-exotoxin producers. The native strains shared similar morphology of parasporal crystals, similar protein pattern and identical insecticidal gene profiles. These features resembled Lepidoptera-toxic strains. Furthermore...
Source: Revista Argentina de Microbiologia - May 14, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Fungal endophytes isolated from Protium heptaphyllum and Trattinnickia rhoifolia as antagonists of Fusarium oxysporum.
Authors: Fierro-Cruz JE, Jiménez P, Coy-Barrera E Abstract Control of fungal pathogens is mainly addressed by the use of chemically synthesized fungicides which result in environmental pollution, developing resistance after prolonged use. In this context, endophytes have been recognized as potential biocontrollers, and also as a promising source of antifungal metabolites. Therefore, as part of our research on phytopathogen controllers, 355 fungal endophytes were isolated from Protium heptaphyllum and Trattinnickia rhoifolia (Burseraceae), both ethnobotanically important tree species that produce secondary me...
Source: Revista Argentina de Microbiologia - May 14, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Plasmid-mediated colistin resistance in Escherichia coli recovered from healthy poultry.
Authors: Dominguez JE, Figueroa Espinosa RA, Redondo LM, Cejas D, Gutkind GO, Chacana PA, Di Conza JA, Fernández-Miyakawa ME PMID: 28457652 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - May 2, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Prevalence of honey bee (Apis mellifera) viruses in temperate and subtropical regions from Argentina.
Authors: Molineri AI, Pacini A, Giacobino A, Bulacio-Cagnolo N, Aignasse A, Zago L, Fondevila N, Ferrufino C, Merke J, Orellano E, Bertozzi E, Pietronave H, Signorini ML Abstract In Argentina, bee virus studies are still incipient, and there are no studies regarding the climatic effect. The aim of this study was to assess and compare the presence of honeybee viruses in different climatic regions from Argentina. A total of 385 colonies distributed in five Argentinean eco-regions were examined to evaluate the percentage of infestation with Varroa destructor and the presence of seven virus species (Deformed wing virus...
Source: Revista Argentina de Microbiologia - May 1, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Multicausal etiology of the enteric syndrome in rabbits from Mexico.
Authors: García-Rubio VG, Bautista-Gómez LG, Martínez-Castañeda JS, Romero-Núñez C Abstract Enteropathies in rabbits are difficult to diagnose; their etiology involves pathogens that act synergistically, causing damage to the intestine. The aim of the present study was isolate enteric pathogens from rabbits in Mexico. Using parasitological, bacteriological and molecular analyses, we screened 58 samples of the intestinal content of rabbits having a clinical history of enteric disease from the southeastern part of the State of Mexico. Out of the 58 samples analyzed, a total o...
Source: Revista Argentina de Microbiologia - April 24, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Ligninolytic enzyme production by white rot fungi during paraquat (herbicide) degradation].
This study evaluated the degradation effect of paraquat on the production of ligninolytic enzymes by white rot fungi isolated from the South of Mexico. Six fungal strains showed tolerance to the herbicide in solid culture. Three of the six evaluated strains showed levels of degradation of 32, 26 and 47% (Polyporus tricholoma, Cilindrobasidium laeve and Deconica citrispora, respectively) after twelve days of cultivation in the presence of the xenobiotic. An increase in laccase and manganese peroxidase (MnP) activities was detected in the strains showing the highest percentage of degradation. Experiments were done with enzym...
Source: Revista Argentina de Microbiologia - April 24, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Campylobacter jejuni O:19 serotype in Argentine poultry meat supply chain].
Authors: Rossler E, Fuhr EM, Lorenzón G, Romero-Scharpen A, Berisvil AP, Blajman JE, Astesana DM, Zimmermann JA, Fusari ML, Signorini ML, Soto LP, Frizzo LS, Zbrun MV Abstract Thermotolerant species of Campylobacter have been focus of attention in the last years because they are the major agent causing zoonotic foodborne diseases. In addition, Campylobacter jejuni O:19 serotype was associated with Guillain Barré syndrome. The aim of this study was to determine the proportion of C. jejuni O:19 serotype isolated at different stages of three poultry meat supply chain in Santa Fe, Argentina. The analysis ...
Source: Revista Argentina de Microbiologia - April 24, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Description of 3 cases of skin and soft tissue infections caused by Streptococcus pneumoniae].
We describe here three cases of SSTI due to S. pneumoniae in patients admitted to the Provincial Pediatric Hospital of Misiones, Argentina that were detected during 10 years of invasive disease (ID) surveillance documented in 2010, 2011 and 2015. These cases involved two girls aged 8 and 7 months old, and a two-year-old male child with diagnoses of gluteal abscess, preseptal cellulites and pyoderma respectively. All the patients were eutrophic and in good general condition on admission; one of them was seropositive for HIV. Antimicrobial susceptibility and serotypes were framed within the local epidemiology of invasive pne...
Source: Revista Argentina de Microbiologia - April 19, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Fagotherapy: Multi-drug resistance compel us to review the past].
Authors: Lopardo HA PMID: 28385179 [PubMed - in process] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - April 9, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Molecular characterization of invasive Streptococcus dysgalactiae subsp. equisimilis. Multicenter study: Argentina 2011-2012.
The objectives of this study were: to know the prevalence of SDSE invasive infections in Argentina, to study the genetic diversity, to determine the presence of virulence genes, to study antibiotic susceptibility and to detect antibiotic resistance genes. Conventional methods of identification were used. Antibiotic susceptibility was determined by the disk diffusion and the agar dilution methods and the E-test. Twenty eight centers from 16 Argentinean cities participated in the study. Twenty three isolates (16 group G and 7 group C) were obtained between July 1 2011 and June 30 2012. Two adult patients died (8.7%). Most of...
Source: Revista Argentina de Microbiologia - March 29, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Biochemical basis of tolerance to osmotic stress in phytopathogenic fungus: The case of Macrophomina phaseolina (Tassi) Goid.]
Authors: Martínez-Villarreal R, Garza-Romero TS, Moreno-Medina VR, Hernández-Delgado S, Mayek-Pérez N Abstract Fungus Macrophomina phaseolina (Tassi) Goid. is the causative agent of charcoal rot disease which causes significant yield losses in major crops such as maize, sorghum, soybean and common beans in Mexico. This fungus is a facultative parasite which shows broad ability to adapt itself to stressed environments where water deficits and/or high temperature stresses commonly occur. These environmental conditions are common for most cultivable lands throughout Mexico. Here we describe some b...
Source: Revista Argentina de Microbiologia - March 29, 2017 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research