Bacterial diversity using metagenomics of 16s rDNA in water kefir, an innovative source of probiotics for bee nutrition
In this study, a high-throughput sequencing technique was used to characterize the bacterial composition of the original water kefir from which potential probiotics were obtained. We studied the bacterial diversity of both water kefir grains and beverages. DNA was extracted from three replicate samples of both grains and beverages using the Powerlyzer Microbial Kit. The hypervariable V1-V2 region of the bacterial 16S ribosomal RNA gene was amplified to prepare six DNA libraries. Between 1.4M and 2.4M base-pairs were sequenced for the library. In total, 28721971 raw reads were obtained from all the samples. Estimated specie...
Source: Revista Argentina de Microbiologia - January 25, 2024 Category: Microbiology Authors: Mar ía A Rodríguez Leticia A Fern ández Marina L D íaz Cristian A Gallo Miguel Corona Jay D Evans Francisco J Reynaldi Source Type: research

Distribution, seroprevalence and risk factors for bovine brucellosis in Brazil: Official data, systematic review and meta-analysis
Rev Argent Microbiol. 2024 Jan 3:S0325-7541(23)00109-8. doi: 10.1016/j.ram.2023.08.002. Online ahead of print.ABSTRACTBovine brucellosis is an endemic disease in Brazil, and evidence-based assessments of the available literature on its seroprevalence and risk factors are limited. The aim of this study was to systematically review and summarize studies related to seroprevalence and risk factors of bovine brucellosis in the entire Brazil, in addition to comparing published data with the most recent official reports. Articles available in scientific databases and published between October 2006 and October 2021 were evaluated....
Source: Revista Argentina de Microbiologia - January 4, 2024 Category: Microbiology Authors: Isis Daniele Dos Santos Rocha In ácio José Clementino D ébora Luise Canuto de Sousa Clebert Jos é Alves Carolina de Sousa Am érico Batista Santos S érgio Santos de Azevedo Source Type: research

Human parasites in surface water used for recreation in Salta, Argentina
In this study, two recreational aquatic environments were studied in the province of Salta, Argentina. Water samples collected from three different locations, two from a creek and one from the outlet of a thermal complex, were monitored at four time points. Physicochemical and microbiological characterization of each point was conducted, as well as a search for parasites and amebae. Parasites were identified through optical microscopy observations and free-living amebae (FLA) were isolated by spiking in Petri dishes followed by subsequent molecular identification. Water samples from the outlet of the thermal complex showed...
Source: Revista Argentina de Microbiologia - December 28, 2023 Category: Microbiology Authors: Diego G Sanguino-Jorquera Hugo R Poma Ver ónica B Rajal Mar ía M Juárez Ver ónica P Irazusta Source Type: research

The MVA vector expressing the F protein of bovine respiratory syncytial virus is immunogenic in systemic and mucosal immunization routes
This study suggests that MVA-F is a good candidate for further evaluations combining intranasal and intramuscular routes, in order to induce local and systemic immune responses, to improve the vaccine efficacy against BRSV infection.PMID:38143232 | DOI:10.1016/j.ram.2023.07.006 (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - December 24, 2023 Category: Microbiology Authors: Alejandra Ferella Marina Mozgovoj D ébora Garanzini Mar ía José Dus Santos Gabriela Calamante Mar ía Paula Del Médico Zajac Source Type: research