[Reportable mycoses: Utopia or reality. A forgotten matter still unresolved].
Authors: Fernandez NB, Davel G PMID: 31097349 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - May 18, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Five-assay microbiological system for the screening of antibiotic residues.
Authors: Tumini M, Nagel OG, Althaus RL Abstract A novel microbiological system in microtiter plates consisting of five bioassays is presented for the detection and classification of antibiotic residues in milk. The bioassays were optimized for the detection of beta-lactams (Bioassay B: Geobacillus stearothermophilus), macrolides (Bioassay M: Bacillus megaterium with fusidic acid), tetracyclines (Bioassay T: B. megaterium with chloramphenicol), quinolones (Bioassay Q: Bacillus licheniformis) and sulfamides (Bioassay QS: B. licheniformis with trimethoprim) at levels near the maximum residue limits (MRL). The respons...
Source: Revista Argentina de Microbiologia - May 7, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Comparison of three molecular subtyping techniques for Listeria monocytogenes.
Authors: Londero A, Costa M, Sucari A, Leotta G Abstract Listeria monocytogenes is a foodborne pathogen. The recent alert for L. monocytogenes in vegetables from Argentina warns about the importance of reinforcing its isolation, characterization and subtyping in food, clinical and environmental samples. The aim of the present study was to compare the discriminatory power of enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR), automated ribotyping and pulsed-field gel electrophoresis (PFGE) to subtype strains of L. monocytogenes isolated from Argentine meat and environmental samples....
Source: Revista Argentina de Microbiologia - April 28, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Experimental infection of sheep with Bovine leukemia virus (BLV): Minimum dose of BLV-FLK cells and cell-free BLV and neutralization activity of natural antibodies.
In this report, we aimed to determine the minimum amount of FLK-BLV cells or cell-free supernatant containing BLV needed to produce infection in sheep. We also evaluated the amount of antibodies obtained from a naturally-infected cow required to neutralize this infection. We observed that both sheep experimentally inoculated with 5000 FLK-BLV cells became infected, as well as one of the sheep receiving 500 FLK-BLV cells. None of the animals inoculated with 50 FLK-BLV cells showed evidence of infection. The cell-free FLK-BLV supernatant proved to be infective in sheep up to a 1:1000 dilution. Specific BLV antibodies showed ...
Source: Revista Argentina de Microbiologia - April 28, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Seroprevalence of leptospirosis in human groups at risk due to environmental, labor or social conditions.
Authors: Meny P, Menéndez C, Ashfield N, Quintero J, Rios C, Iglesias T, Schelotto F, Varela G Abstract Leptospirosis is important in Uruguay due to the economic loss caused by the diseases of production animals, mainly bovines, and also due to frequent human infection. We decided to study anti-Leptospira antibodies in the sera of dairy workers, rice laborers, veterinarians, suburban slum dwellers and garbage recyclers. Our aims were to estimate the seroprevalence of infection by Leptospira spp. in these people at risk, the relative importance of the known risk factors associated with infection, and the impa...
Source: Revista Argentina de Microbiologia - April 15, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Carbon-substrate utilization profiles by Cladorrhinum (Ascomycota).
Authors: Barrera VA, Martin ME, Aulicino M, Martínez S, Chiessa G, Saparrat MCN, Gasoni AL Abstract Fungi from the genus Cladorrhinum (Ascomycota) are promising agents in the biocontrol of phytopathogens, in the promotion of plant growth, and in the production of enzymes with technological application. We analyzed comparatively the ability of 5 native strains of Cladorrhinum samala and Cladorrhinum bulbillosum with reference strains belonging to the same genus. We used 95 individual carbon sources available in microplates from the Biolog® FF system. Although most of the strains mainly used soluble carboh...
Source: Revista Argentina de Microbiologia - April 15, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Detection of Human T lymphotropic virus 1 (HTLV-1) Cosmopolitan subtype Transcontinental subgroup (Aa) and HTLV-2 subtype b in blood donors of Corrientes].
Authors: Borda MA, Svibel GR, Biglione MM, Berini CA Abstract A molecular epidemiological study was conducted in a population of 9422 blood donors in the province of Corrientes, Northeastern Argentina, to determine the prevalence of Human T-cell lymphotropic virus types 1 and 2 (HTLV-1/2), the phylogenetic identification of HTLV-1 and 2 subtypes/subgroups and perform a mutation analysis. Based on the results obtained, it was shown that both HTLV-1 and HTLV-2 are circulating in a low-risk population of Corrientes, although with a similar prevalence to that of non-endemic areas. Phylogenetic studies identified the HT...
Source: Revista Argentina de Microbiologia - April 4, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Spotted fever in Argentina. Description of two clinical cases].
Authors: Armitano RI, Guillemi E, Escalada V, Govedic F, Lopez JL, Farber M, Borras P, Prieto M Abstract Rickettsioses are zoonotic tick-borne diseases. In Argentina, there are two epidemiological scenarios: jungle of Salta and Jujuy, involving vectors from the "Amblyomma cajennense Complex" (A. sculptum, and A. toneliae) and Rickettsia rickettsii as the main etiological agent; and the second scene to Delta del Rio Paraná and Samborombón Bay, where Amblyomma triste acts as a vector; and the provinces of Córdoba, La Rioja, San Luis and La Pampa where Amblyomma tigrinum is the vector. I...
Source: Revista Argentina de Microbiologia - April 1, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[What can we do to prevent multidrug-resistance?]
Authors: Nastro M PMID: 30904052 [PubMed - in process] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - March 26, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Correlation between ammonia-oxidizing microorganisms and environmental factors during cattle manure composting.
Authors: Sun Y, Zhu L, Xu X, Meng Q, Men M, Xu B, Deng L Abstract Cattle manure composting was performed in an aerated vessel. Community structure and diversity of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) were investigated using polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) techniques targeting the ammonia monooxygenase alpha subunit (amoA) gene and the correlation between AOB and AOA communities and environmental factors was explored. Thirteen (13) AOB sequences were obtained, which were closely related to Nitrosomonas spp., Nitrosomonas eutropha, and ...
Source: Revista Argentina de Microbiologia - March 26, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Weissella paramesenteroides encapsulation and its application in the use of fish waste.
Authors: Libonatti C, Agüeria D, García C, Basualdo M Abstract The goal of the present study was to evaluate the encapsulation of Weissella paramesenteroides, isolated from bee bread, as a technological tool for its use in biological fish silage. The pH decrease in fish silages using the bacteria encapsulated and in a non-encapsulated form was compared. W. paramesenteroides showed a good performance in the development of biological fish silage. The alginate encapsulation method showed an encapsulation efficacy of 85% and provides a reliable technological application. PMID: 30905352 [PubMed - in proc...
Source: Revista Argentina de Microbiologia - March 26, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

The bZIP transcription factor Afap1 mediates the oxidative stress response and aflatoxin biosynthesis in Aspergillus flavus.
Authors: Guan X, Zhao Y, Liu X, Shang B, Xing F, Zhou L, Wang Y, Zhang C, Bhatnagar D, Liu Y Abstract Aflatoxin is a carcinogenic secondary metabolite produced mainly by Aspergillus flavus and Aspergillus parasiticus, which can seriously endanger the health of humans and animals. Oxidative stress is a common defense response, and it is known that reactive oxygen species (ROS) can induce the synthesis of a series of secondary metabolites, including aflatoxin. By using mutants lacking the afap 1 gene, the role of afap1 gene in oxidative stress and aflatoxin synthesis was assessed. The growth of the mutant strains was...
Source: Revista Argentina de Microbiologia - March 26, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Food poisoning outbreak in a kindergarten in the Province of Buenos Aires, Argentina].
Authors: Manfredi EA, Rivas M Abstract On November 27, 2008, a foodborne disease outbreak associated with the consumption of chicken salad occurred in a kindergarten in the District of Hurlingham, Province of Buenos Aires. Thirty-seven children and 10 adults with gastrointestinal symptoms were affected. Five children were hospitalized with signs of dehydration, one of them requiring intensive care. Staphylococcus aureus subsp. aureus was isolated from the mentioned food in 4 out of 5 stool specimens from the patients, and in 3 out of 5 food handlers (nose of food handler #1, hands of food handlers #2 and 3). The is...
Source: Revista Argentina de Microbiologia - March 21, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

In vitro effect of the crude extract of a potato common scab streptomycete in Sinaloa, Mexico.
This study reports that Streptomyces sp. V2 has a toxigenic region (TR) that is associated with the thaxtomin gene cluster. PMID: 30799233 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - February 28, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Corynebacterium pseudotuberculosis biovar ovis: Evaluation of antibiotics susceptibility in vitro].
Authors: Gallardo AA, Toledo RA, González Pasayo RA, Azevedo V, Robles C, Paolicchi FA, Estevao Belchior SG Abstract The aims of this work were to study the antibiotic susceptibility in Corynebacterium pseudotuberculosis isolated from small ruminants and to determine the presence of integrons that contain resistance genes. Fifteen isolates of different sources were analysed using the diffusion and the dilution methods. When the diffusion method was performed, amoxicillin-clavulanic, ampicillin, cefotaxime, cefoxitin, ciprofloxacin, chloramphenicol, erythromycin, streptomycin, gentamicin, imipenem, kanamycin,...
Source: Revista Argentina de Microbiologia - February 26, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Identification of an Argentinean isolate of Spodoptera frugiperda granulovirus.
Authors: Pidre ML, Sabalette KB, Romanowski V, Ferrelli ML Abstract The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is an important maize pest. Due to the environmental impact and emergence of resistance caused by chemical pesticides and transgenic events, the use of baculoviruses becomes a safe and useful alternative for its control in integrated pest management strategies. Here we report the identification of a novel isolate of a granulovirus of S. frugiperda native to the central region of Argentina, named SfGV ARG. We observed that larvae infected with SfGV ARG showed a yellowish...
Source: Revista Argentina de Microbiologia - February 25, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Synthesis and regulation of flavor compounds derived from brewing yeast: fusel alcohols].
Authors: Loviso CL, Libkind D Abstract Among the main beer components, fusel alcohols are important because of their influence on the flavor of the final product, and therefore on its quality. During the production process, these compounds are generated by yeasts through the metabolism of amino acids. The yeasts, fermentation conditions and wort composition affect fusel alcohols profiles and their concentrations. In this review, we provide detailed information about the enzymes involved in fusel alcohols formation and their regulation. Moreover, we describe how the type of yeast used, the fermentation temperature a...
Source: Revista Argentina de Microbiologia - February 6, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Effects of the culture medium and the methodology applied on the biofilm formation of 2diarrheagenic Escherichia coli strains].
Authors: Cáceres ME, Etcheverría AI, Padola NL Abstract The ability to form biofilms of pathogenic microorganisms in a wide variety of environments, surfaces and conditions constitute an important risk, both for the food industry and for public health. The aim of this work was to evaluate and to compare the effects of the methodology applied and the culture medium used on the ability of a non-O157 verotoxigenic Escherichia coli strain and an enteropathogenic strain to form biofilm on polystyrene surface. Two methodological variants were tested in static culture and culture mediums with different compo...
Source: Revista Argentina de Microbiologia - January 24, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

RNA-seq analysis of the response of plant-pathogenic oomycete Phytophthora parasitica to the fungicide dimethomorph.
Authors: Hao K, Lin B, Nian F, Gao X, Wei Z, Luo G, Lu Y, Lan M, Yang J, Wu G Abstract Phytophthora parasitica is an important oomycete that causes disease in a variety of plants, dimethomorph fungicides being specific for oomycetes. The aim of this study was to use RNA-seq to rapidly discover the mechanism by which dimethomorph acts in the treatment of P. parasitica. We found that the expression of 832 genes changed significantly after the dimethomorph treatment, including 365 up-regulated genes and 467 down-regulated genes. According to the Gene Ontology (GO) enrichment analysis, pathway enrichment and verificati...
Source: Revista Argentina de Microbiologia - January 24, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Endophytic microorganisms Agrobacterium tumefaciens 6N2 and Meyerozyma guilliermondii 6N serve as models for the study of microbial interactions in colony biofilms.
Authors: Bertini EV, Leguina ACDV, Castellanos de Figueroa LI, Nieto-Peñalver CG PMID: 30651186 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - January 19, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Genetic diversity of Mycoplasma hyopneumoniae in Mendoza province.
Authors: Sosa C, Blois A, Ibáñez F, Tamiozzo P Abstract In Argentina, enzootic pneumonia (EP) is highly prevalent and different genetic types of Mycoplasma hyopneumoniae have been identified. However, there is a lack of information about prevalence and other epidemiological aspects of EP in Mendoza province. A multiple Locus variable-number tandem repeat analysis (MLVA) targeting P97 R1, P97 R1A and P146 R3 loci was used to assess the genetic diversity of M. hyopneumoniae from clinical specimens recovered from pigs from five herds located in different districts of Mendoza province. M. hyopneumoniae co...
Source: Revista Argentina de Microbiologia - January 19, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Yeast diversity in Vitis non-vinifera ecosystems.
Authors: Raymond Eder ML, Rosa AL Abstract The surface of grapes lodges a complex community of yeast species responsible for spontaneous alcoholic fermentation. The study of indigenous Saccharomyces and "non-Saccharomyces" yeasts during grape must fermentation constitutes a major research area in microbial enology. Although there are detailed studies on the microbiota of Vitis vinifera L. grapes, little is known about the diversity of yeast communities present in non-vinifera Vitis ecosystems (i.e., grapes and spontaneously fermenting grape musts). Potentially scientific and/or enological valuable yeast s...
Source: Revista Argentina de Microbiologia - January 16, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Detection of serovars of Salmonella in artificially reared calves in Mar y Sierras Dairy Basin, Argentina].
In conclusion, 11 Salmonella serovars were detected in one out of 3dairy farms in Mar and Sierras Dairy Basin, and not only were these serovars associated with diarrhea signs including the presence of mucus in feces, but they were also more prevalent among calves aged up to 21 days. PMID: 30638637 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - January 16, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Clinical and microbiological characteristics of community-acquired pneumonia associated with Streptococcus pneumoniae in adult patients in Mexico.
In conclusion, head trauma was the most common comorbidity in the CAP group. The most prevalent serotype was 19A. Decreased susceptibility to most antimicrobials tested was observed. PMID: 30635204 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - January 14, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Electronic cardiovascular device-associated to infective endocarditis caused by Mycobacterium mageritense].
We describe a case of endocarditis caused by Mycobacterium mageritense in a patient with an autologous pericardial patch and a pressure catheter in the left auricle. The bacterial species was identified as Mycobacterium mageritense by mass spectrometry (MALDI-TOF MS), score 2.3, and confirmed by 16S rRNA analysis with 99.8 and 100% agreement, respectively. PMID: 30591316 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - December 30, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

National production of certified reference fungal cultures.
Authors: Davel GO, Mazza M, Refojo N, Taverna CG, Rivas MC, Fernández J, Vivot ME, Mazza MG, Mastromónaco GM, Bueno NS, Molina V, Brero ML, Kornblit F Abstract Reference fungal cultures (RFCs) are essential for the internal quality control of laboratories. The production of these cultures requires standardized procedures (IRAM 14950:2016 and ISO 17034:2016 standards) carried out by a recognized and accredited laboratory. The aim of this work was to produce RFC in paper disks of autochthonous strains, characterized by two, homogeneous and stable reference methods traceable at species level. RFC were pr...
Source: Revista Argentina de Microbiologia - December 30, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Species diversity of non-tuberculous mycobacteria isolated from  aquatic environments of General Pico city, Province of La Pampa (Argentina)].
[Species diversity of non-tuberculous mycobacteria isolated from aquatic environments of General Pico city, Province of La Pampa (Argentina)]. Rev Argent Microbiol. 2018 Dec 19;: Authors: Tortone CA, Oriani DS, Staskevich AS, Oriani AS, Gino LM, Marfil MJ, Nava Vargas A, Gioffré AK, Zumárraga MJ Abstract Non-tuberculous mycobacteria (NTM) are studied not only for their importance as emerging opportunistic pathogens but also for their applications in biotechnology and bioremediation. Our aim was to determine the occurrence and diversity of mycobacteria in different aquatic habitats of...
Source: Revista Argentina de Microbiologia - December 25, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Zoonosis and veterinary waste disposal in rural practice.
The objectives of this study were to estimate: (a) the frequency of zoonoses in large animal veterinarians from rural areas of the province of Buenos Aires, Argentina, and (b) to describe the use and disposal of personal protective equipment (PPE) and selective veterinary clinical waste. A cross-sectional study was carried out on large animal veterinary practitioners in the Province of Buenos Aires (n=106). One third (29.2%) of them had been diagnosed with a zoonosis by laboratory-methods, being brucellosis the most frequent (22.6%). The more years passed since their graduation, the greater the chances of becoming ill (p
Source: Revista Argentina de Microbiologia - December 20, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Solid state fermentation to obtain vegetable products bio-enriched with isoflavone aglycones using lactic cultures.
Authors: Correa Deza MA, Rodríguez de Olmos A, Garro MS Abstract The consumption of soybean isoflavones (IS) is associated with several beneficial properties on human health. Some lactic acid bacteria possess β-glucosidase enzyme, that allows to obtain the active form of IS (aglycone). The solid state fermentation (SSF) has received great attention in the last years in order to obtain several valuable compounds. SSF, using soybean as substrate and Lactobacillus rhamnosus CRL 981 as starter, was studied in the present work. Sucrose was added into soybean paste to study the effect on the behavior of the s...
Source: Revista Argentina de Microbiologia - December 20, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Development and evaluation of an in-house database for quick identification of Burkholderia contaminans by MALDI-TOF MS].
Authors: Cipolla L, Rocca F, Armitano RI, Martinez C, Almuzara M, Faccone D, Vay C, Prieto M Abstract MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry (MS) proved to be a robust tool for the identification of numerous taxonomic groups. However, it has limitations. A key advantage of this technique is the flexibility for the incorporation of protein profiles of microorganisms not included in the commercial database. Due to the prevalence of Burkholderia contaminans in fibrocystic patients in Argentina and the fact that rapid and reliable microbiological diagnosis is crucial in...
Source: Revista Argentina de Microbiologia - December 20, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Molecular epidemiology of carbapenem-resistant isolates of Acinetobacter baumannii in Argentina].
Authors: H Rodríguez C, Nastro M, A Flores S, Rodriguez M, Spinozzi M, Bruni G, L López A, David V, S Aiassa M, A Marqués I, R Navarro O, Paniccia L, Famiglietti A, Grupo colaborativo Acinetobacter Argentina Abstract One hundred sequential, epidemiologically unrelated carbapenem-resistant- Acinetobacter baumannii isolates from 11 hospitals in 10 Argentine provinces were collected between January and August 2016. Genes coding for Ambler class D and B carbapenemases were investigated by PCR using specific primers. All isolates were typed using the 3-locus sequence typing and blaOXA-51-like sequen...
Source: Revista Argentina de Microbiologia - December 17, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Identification of Brucella abortus biovar 4 of bovine origin in Colombia.
The objective of this study was to identify twelve Brucella abortus isolates of bovine origin from the department of Nariño in Colombia up to the biovar level. These isolates are included in the collection of the Germplasm Bank of Microorganisms of Animal Health Interest - Bacteria and Virus (BGSA-BV). The identification was carried out through conventional methods such as macro and microscopic morphological descriptions, enzymatic activity, biochemical profile, substrate use and sensitivity to dyes. Complementary genotypic characterization was carried out using multiplex PCR for B. abortus, Brucella melitensis, Bru...
Source: Revista Argentina de Microbiologia - December 17, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Microbiological analysis of the maternal genital tract and umbilical cord blood and its association with neonatal damage].
The objective of the study was to identify microorganisms of the maternal genital tract that are associated with neonatal damage, in order to prevent future perinatal complications. Seven hundred and eleven pregnant patients attended their prenatal control during the period January 2010-July 2013. Ureaplasma urealyticum and Mycoplasma hominis presence was investigated in umbilical cord blood by metabolic substrates (Micofast-Biomerieux) and that of T.vaginalis, by PCR using specific primers. The microbiological study of the vaginal contents of 288 pregnant patients at weeks 35 to 37 was performed by conventional methods, a...
Source: Revista Argentina de Microbiologia - December 15, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Predatory journals: An evil of this time?]
Authors: Sauka DH PMID: 30482270 [PubMed - in process] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - December 3, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Diversity and abundance of denitrifiers during cow manure composting.
Authors: Meng Q, Xu X, Zhang W, Cheng L, Men M, Xu B, Deng L, Sun X Abstract Diversity and abundance of the denitrifying genes nirK, nirS and nosZ were investigated in cow manure compost using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and real-time quantitative PCR (qPCR), respectively. These three genes were detected in all the stages of the composting process. Phylogenetic analysis showed that the nirK gene was closely related to Rhizobiales, Burkholderiales, the nirS gene was closely related to Pseudomonadales and Burkholderiales, and the nosZ gene was closely related to Rhodos...
Source: Revista Argentina de Microbiologia - November 24, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Immunoprotective response induced by recombinant glycoprotein D in the BALB/c respiratory mouse model of Equid alphaherpesvirus 1 infection.
Authors: Fuentealba NA, Sguazza GH, Zanuzzi CN, Bravi ME, Scrochi MR, Valera AR, Corva SG, Gimeno EJ, Pecoraro MR, Galosi CM Abstract Equid alphaherpesvirus 1 (EHV-1) infection causes abortion, respiratory disease, perinatal deaths and neurological disorders in horses. The natural infection and available vaccines provide only partial and short-lived protection against reinfections. In the present study, we analyzed the ability of purified baculovirus-expressed glycoprotein D (gD) administered by different routes to induce protective immunity in BALB/c mice after challenge with the EHV-1 AR8 strain. Clinical signs v...
Source: Revista Argentina de Microbiologia - November 4, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Histoplasma capsulatum electron microscopy isolated from a patient with disseminated histoplasmosis].
Authors: Pedersen D, Fiore C, Arechavala A, Paniccia L, Occhionero M PMID: 30377014 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - November 1, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Isolation and characterization of a marine testosterone-degrading bacterium: Vibrio sp. N3.
In this study, a marine testosterone-degrading bacterium (strain N3) was isolated from Nanao Island in the South China Sea. In addition, the strain could also use 17β-estradiol (E2), 17β-ethinyl estradiol (EE2), estriol (E3) or cholesterol as a sole carbon source. According to the 16S rRNA gene sequence analysis, strain N3 was identified as Vibrio sp. Further characterization showed that the strain is aerobic, gram-negative, and mobile and exhibits resistance to ampicillin, carbenicillin, penicillin and spectinomycin. For enhancing its capacity of testosterone degradation, the Plackett-Burman factorial design and...
Source: Revista Argentina de Microbiologia - October 11, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Atypical hand, foot, and mouth disease caused by Coxsackievirus A6 in Argentina in 2015.
We present two groups of cases of atypical hand, foot, and mouth disease (HFMD) caused by Coxsackievirus A6 (CV-A6) detected in Argentina in 2015. The first group involved 14 patients from Chubut province and the second group affected 12 patients from San Luis province. Molecular analysis of the complete VP1 protein gene revealed the circulation of E2 sublineage, the most predominant worldwide. To our knowledge, this is the first report of CV-A6 infections associated with atypical HFMD in Argentina and South America. PMID: 30269935 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - October 4, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Clinical and epidemiological characteristics of patients with paracoccidioidomycosis diagnosed in a hospital of Resistencia, Chaco].
Authors: Tracogna MF, Fernández Lugo S, Gariboglio Vázquez ML, Fernández MS, Andriani ME, Presti SE, Arce V, López R, Iliovich E, Marques IA, Cattana ME Abstract In order to describe the clinical and epidemiological characteristics of paracoccidioidomycosis, a descriptive study of all the cases diagnosed by the Clinical Microbiology Service at Dr. Julio C. Perrando hospital in the city of Resistencia (Chaco Province, Argentina) was conducted. Between 2011 and 2014, 46 cases were detected. In the period 2013-2014, an almost 4-fold increase in the incidence rate was detected. The chronic f...
Source: Revista Argentina de Microbiologia - September 25, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Resistance to β-lactams in enterococci.
Resistance to β-lactams in enterococci. Rev Argent Microbiol. 2018 Sep 19;: Authors: Gagetti P, Bonofiglio L, García Gabarrot G, Kaufman S, Mollerach M, Vigliarolo L, von Specht M, Toresani I, Lopardo HA Abstract Enterococci are intrinsically resistant to several antimicrobial classes and show a great ability to acquire new mechanisms of resistance. Resistance to β-lactam antibiotics is a major concern because these drugs either alone or in combination are commonly used for the treatment of enterococcal infections. Ampicillin resistance, which is rare in Enterococcus faecalis, occurs i...
Source: Revista Argentina de Microbiologia - September 25, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Observation of interactions between hyphae of Fusarium verticillioides, Aspergillus flavus and Talaromyces funiculosus in microcultures developed in hermetic environments].
Authors: Castellari CC, Marcos Valle FJ, Pacin AM PMID: 30243526 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - September 25, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Helicobacter cinaedi bacteremia: Presentation of the first cases reported in Argentina].
In this report we present the first documented cases of bacteremia caused by H. cinaedi in Argentina. PMID: 30243527 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - September 25, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Acute disseminated encephalomyelitis due to Mycoplasma pneumoniae in a previously healthy boy].
We present here the case of a previously healthy 5 year-old boy hospitalized in an intensive care unit due to tonic-clonic seizures focused on the face and right side of the body, and axillary temperature of 37.4°C. Common bacterial and viral etiology was ruled out through studies of cerebrospinal fluid (CSF) samples. Mycoplasma pneumoniae was suspected by a positive immunofluorescence serum test for IgM class antibodies. Finally, with a brain biopsy, M. pneumoniae was confirmed by polymerase chain reaction (PCR) and acute disseminated encephalomyelitis by pathological anatomy. The patient was treated with clarithromyc...
Source: Revista Argentina de Microbiologia - September 22, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Bacterial diversity in the Cr(VI) reducing biocathode of a Microbial Fuel Cell with salt bridge.
In this study, an efficient biocathode for the reduction of Cr(VI) with future use in bioremediation, was characterized. PMID: 30144991 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - August 28, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Antifungal and antimycotoxigenic effect of Lactobacillus plantarum CRL 778 at different water activity values.
In this study, the ability of Lactobacillus (L.) plantarum CRL 778 to control growth and OTA production by Aspergillus (A.) niger 13D strain, at different water activity (aw) values (0.955, 0.964, 0.971, 0.982, and 0.995) was determined in vitro. Both parameters were significantly (p
Source: Revista Argentina de Microbiologia - August 28, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Infective endocarditis due to non-HACEK gram-negative bacilli in a Level III cardiovascular center in Argentina (1998-2016)].
Authors: Burgos LM, Oses P, Iribarren AC, Pennini M, Merkt M, Vrancic M, Camporrotondo M, Ronderos R, Sucari A, Nacinovich F Abstract Non-HACEK Gram-negative bacilli are a rare cause of infective endocarditis. Epidemiological, diagnostic and prognostic aspects of this entity are little known, and there is limited experience. The aim of this study was to analyze the clinical, microbiological and in-hospital outcomes of non-HACEK Gram negative bacilli endocarditis and to compare them with those due to other microorganisms. PMID: 30143351 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - August 26, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Persist and triumph: persistent cells in microbial biofilm].
Authors: Paraje MG PMID: 30057356 [PubMed - in process] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - August 1, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Growth inhibition and genetic modification of Phytophthora capsici using chitosan with low degree of polymerization].
Authors: Ramírez-Benítez JE, Arjona Sabido RA, Caamal Velázquez JH, Rodríguez Ávila NL, Solís Pereira SE, Lizama Uc G Abstract Phytophthora blight of peppers, caused by oomycete Phytophthora capsici, currently causes economic losses in crops such as peppers, tomatoes, eggplant and cucurbits. In this work, we evaluated the effect of chitosan with low degree of polymerization (LDP) on growth and gene expression of P. capsici cultures. LDP chitosan inhibited 88% of P. capsici mycelial growth at concentrations up to 0,4 mg/l, whereas at concentrations higher than 1,6 mg/l it co...
Source: Revista Argentina de Microbiologia - July 26, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Silver nanoparticles from Justicia spicigera and their antimicrobial potentialities in the biocontrol of foodborne bacteria and phytopathogenic fungi.
Authors: Bernardo-Mazariegos E, Valdez-Salas B, González-Mendoza D, Abdelmoteleb A, Tzintzun Camacho O, Ceceña Duran C, Gutiérrez-Miceli F Abstract In the present work, the biosynthesis of silver-nanoparticles (AgNP) was evaluated using the aqueous extract from Justicia spicigera. The obtained silver nanoparticles were characterized using UV-visible spectroscopic techniques, energy dispersive X-ray spectrometers (EDS), zeta potential and dynamic light scattering. The antimicrobial activity of biosynthesized AgNP was tested against foodborne bacteria (Bacillus cereus, Klebsiella pneumoniae and E...
Source: Revista Argentina de Microbiologia - July 23, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research