Diversity and abundance of denitrifiers during cow manure composting.
Authors: Meng Q, Xu X, Zhang W, Cheng L, Men M, Xu B, Deng L, Sun X Abstract Diversity and abundance of the denitrifying genes nirK, nirS and nosZ were investigated in cow manure compost using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and real-time quantitative PCR (qPCR), respectively. These three genes were detected in all the stages of the composting process. Phylogenetic analysis showed that the nirK gene was closely related to Rhizobiales, Burkholderiales, the nirS gene was closely related to Pseudomonadales and Burkholderiales, and the nosZ gene was closely related to Rhodos...
Source: Revista Argentina de Microbiologia - November 24, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Immunoprotective response induced by recombinant glycoprotein D in the BALB/c respiratory mouse model of Equid alphaherpesvirus 1 infection.
Authors: Fuentealba NA, Sguazza GH, Zanuzzi CN, Bravi ME, Scrochi MR, Valera AR, Corva SG, Gimeno EJ, Pecoraro MR, Galosi CM Abstract Equid alphaherpesvirus 1 (EHV-1) infection causes abortion, respiratory disease, perinatal deaths and neurological disorders in horses. The natural infection and available vaccines provide only partial and short-lived protection against reinfections. In the present study, we analyzed the ability of purified baculovirus-expressed glycoprotein D (gD) administered by different routes to induce protective immunity in BALB/c mice after challenge with the EHV-1 AR8 strain. Clinical signs v...
Source: Revista Argentina de Microbiologia - November 4, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Histoplasma capsulatum electron microscopy isolated from a patient with disseminated histoplasmosis].
Authors: Pedersen D, Fiore C, Arechavala A, Paniccia L, Occhionero M PMID: 30377014 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - November 1, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Isolation and characterization of a marine testosterone-degrading bacterium: Vibrio sp. N3.
In this study, a marine testosterone-degrading bacterium (strain N3) was isolated from Nanao Island in the South China Sea. In addition, the strain could also use 17β-estradiol (E2), 17β-ethinyl estradiol (EE2), estriol (E3) or cholesterol as a sole carbon source. According to the 16S rRNA gene sequence analysis, strain N3 was identified as Vibrio sp. Further characterization showed that the strain is aerobic, gram-negative, and mobile and exhibits resistance to ampicillin, carbenicillin, penicillin and spectinomycin. For enhancing its capacity of testosterone degradation, the Plackett-Burman factorial design and...
Source: Revista Argentina de Microbiologia - October 11, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Atypical hand, foot, and mouth disease caused by Coxsackievirus A6 in Argentina in 2015.
We present two groups of cases of atypical hand, foot, and mouth disease (HFMD) caused by Coxsackievirus A6 (CV-A6) detected in Argentina in 2015. The first group involved 14 patients from Chubut province and the second group affected 12 patients from San Luis province. Molecular analysis of the complete VP1 protein gene revealed the circulation of E2 sublineage, the most predominant worldwide. To our knowledge, this is the first report of CV-A6 infections associated with atypical HFMD in Argentina and South America. PMID: 30269935 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - October 4, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Clinical and epidemiological characteristics of patients with paracoccidioidomycosis diagnosed in a hospital of Resistencia, Chaco].
Authors: Tracogna MF, Fernández Lugo S, Gariboglio Vázquez ML, Fernández MS, Andriani ME, Presti SE, Arce V, López R, Iliovich E, Marques IA, Cattana ME Abstract In order to describe the clinical and epidemiological characteristics of paracoccidioidomycosis, a descriptive study of all the cases diagnosed by the Clinical Microbiology Service at Dr. Julio C. Perrando hospital in the city of Resistencia (Chaco Province, Argentina) was conducted. Between 2011 and 2014, 46 cases were detected. In the period 2013-2014, an almost 4-fold increase in the incidence rate was detected. The chronic f...
Source: Revista Argentina de Microbiologia - September 25, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Resistance to β-lactams in enterococci.
Resistance to β-lactams in enterococci. Rev Argent Microbiol. 2018 Sep 19;: Authors: Gagetti P, Bonofiglio L, García Gabarrot G, Kaufman S, Mollerach M, Vigliarolo L, von Specht M, Toresani I, Lopardo HA Abstract Enterococci are intrinsically resistant to several antimicrobial classes and show a great ability to acquire new mechanisms of resistance. Resistance to β-lactam antibiotics is a major concern because these drugs either alone or in combination are commonly used for the treatment of enterococcal infections. Ampicillin resistance, which is rare in Enterococcus faecalis, occurs i...
Source: Revista Argentina de Microbiologia - September 25, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Observation of interactions between hyphae of Fusarium verticillioides, Aspergillus flavus and Talaromyces funiculosus in microcultures developed in hermetic environments].
Authors: Castellari CC, Marcos Valle FJ, Pacin AM PMID: 30243526 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - September 25, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Helicobacter cinaedi bacteremia: Presentation of the first cases reported in Argentina].
In this report we present the first documented cases of bacteremia caused by H. cinaedi in Argentina. PMID: 30243527 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - September 25, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Acute disseminated encephalomyelitis due to Mycoplasma pneumoniae in a previously healthy boy].
We present here the case of a previously healthy 5 year-old boy hospitalized in an intensive care unit due to tonic-clonic seizures focused on the face and right side of the body, and axillary temperature of 37.4°C. Common bacterial and viral etiology was ruled out through studies of cerebrospinal fluid (CSF) samples. Mycoplasma pneumoniae was suspected by a positive immunofluorescence serum test for IgM class antibodies. Finally, with a brain biopsy, M. pneumoniae was confirmed by polymerase chain reaction (PCR) and acute disseminated encephalomyelitis by pathological anatomy. The patient was treated with clarithromyc...
Source: Revista Argentina de Microbiologia - September 22, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Bacterial diversity in the Cr(VI) reducing biocathode of a Microbial Fuel Cell with salt bridge.
In this study, an efficient biocathode for the reduction of Cr(VI) with future use in bioremediation, was characterized. PMID: 30144991 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - August 28, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Antifungal and antimycotoxigenic effect of Lactobacillus plantarum CRL 778 at different water activity values.
In this study, the ability of Lactobacillus (L.) plantarum CRL 778 to control growth and OTA production by Aspergillus (A.) niger 13D strain, at different water activity (aw) values (0.955, 0.964, 0.971, 0.982, and 0.995) was determined in vitro. Both parameters were significantly (p
Source: Revista Argentina de Microbiologia - August 28, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Infective endocarditis due to non-HACEK gram-negative bacilli in a Level III cardiovascular center in Argentina (1998-2016)].
Authors: Burgos LM, Oses P, Iribarren AC, Pennini M, Merkt M, Vrancic M, Camporrotondo M, Ronderos R, Sucari A, Nacinovich F Abstract Non-HACEK Gram-negative bacilli are a rare cause of infective endocarditis. Epidemiological, diagnostic and prognostic aspects of this entity are little known, and there is limited experience. The aim of this study was to analyze the clinical, microbiological and in-hospital outcomes of non-HACEK Gram negative bacilli endocarditis and to compare them with those due to other microorganisms. PMID: 30143351 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - August 26, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Persist and triumph: persistent cells in microbial biofilm].
Authors: Paraje MG PMID: 30057356 [PubMed - in process] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - July 31, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Growth inhibition and genetic modification of Phytophthora capsici using chitosan with low degree of polymerization].
Authors: Ramírez-Benítez JE, Arjona Sabido RA, Caamal Velázquez JH, Rodríguez Ávila NL, Solís Pereira SE, Lizama Uc G Abstract Phytophthora blight of peppers, caused by oomycete Phytophthora capsici, currently causes economic losses in crops such as peppers, tomatoes, eggplant and cucurbits. In this work, we evaluated the effect of chitosan with low degree of polymerization (LDP) on growth and gene expression of P. capsici cultures. LDP chitosan inhibited 88% of P. capsici mycelial growth at concentrations up to 0,4 mg/l, whereas at concentrations higher than 1,6 mg/l it co...
Source: Revista Argentina de Microbiologia - July 26, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Silver nanoparticles from Justicia spicigera and their antimicrobial potentialities in the biocontrol of foodborne bacteria and phytopathogenic fungi.
Authors: Bernardo-Mazariegos E, Valdez-Salas B, González-Mendoza D, Abdelmoteleb A, Tzintzun Camacho O, Ceceña Duran C, Gutiérrez-Miceli F Abstract In the present work, the biosynthesis of silver-nanoparticles (AgNP) was evaluated using the aqueous extract from Justicia spicigera. The obtained silver nanoparticles were characterized using UV-visible spectroscopic techniques, energy dispersive X-ray spectrometers (EDS), zeta potential and dynamic light scattering. The antimicrobial activity of biosynthesized AgNP was tested against foodborne bacteria (Bacillus cereus, Klebsiella pneumoniae and E...
Source: Revista Argentina de Microbiologia - July 23, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Detection and molecular characterization of Chlamydia psittaci and Chlamydia abortus in psittacine pet birds in Buenos Aires province, Argentina.
This report contributes to the improvement of our understanding of chlamydial agents in our country. PMID: 30017323 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - July 20, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Veil-like pellicle development by Azospirillum brasilense in semisolid NFb medium.
Authors: Di Salvo LP, García de Salamone IE PMID: 30017324 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - July 20, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

The wide distribution of an extremely thermoacidophilic microorganism in the copper mine at ambient temperature and under acidic condition and its significance in bioleaching of a chalcopyrite concentrate.
In this study samples were collected at ambient temperature from Sarcheshmeh Copper Complex in Iran. Mixed samples were inoculated into the culture medium for enrichment of the microorganisms. Pure cultures from these enrichments were obtained by subculture of liquid culture to solid media. Morphological observation was performed under the scanning electron microscope. Isolates were adapted to 30% (w/v) pulp density. For the bioleaching test, the experiments were designed with DX7 software. Bioleaching experiments were carried out in Erlenmeyer flasks and a stirred tank reactor. The highest copper recovery in Erlenmeyer fl...
Source: Revista Argentina de Microbiologia - June 30, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Current scenario of plasmid-mediated colistin resistance in Latin America.
Authors: Quiroga C, Nastro M, Di Conza J Abstract Colistin resistance can occur by chromosomal mutations and by acquisition of plasmid-carrying determinants, mainly mcr-1. In the recent years, we have observed the outburst of this resistance gene in our region. Due to the risk of the rapid dissemination of mcr-1, this finding has worried and alerted different actors from the health field and has become one of the most prolific topics. Our review compiles available reports of well-documented mcr-1-positive strains of Enterobacteriaceae, obtained from different samples in Argentina and other countries of Latin Americ...
Source: Revista Argentina de Microbiologia - June 29, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Frequency, characterization and genotypic analysis of Shiga toxin-producing Escherichia coli in beef slaughterhouses of Argentina.
The objectives of this study were: (1) to estimate STEC frequency in hide and carcass samples taken from beef slaughterhouses supplying the domestic market in Argentina, (2) to establish the pheno-genotypic characteristics of STEC and non-toxigenic Escherichia coli of serogroups O26, O45, O103, O121, O111, O145 or O157 isolated from the analyzed samples and, (3) to study their clonal relatedness. Sixty hides and 60 carcasses were analyzed. At the screening step, 48% of hide and 80% of carcass samples tested positive for the stx gene by endpoint PCR. The STEC isolation rate was 5% for hides and 8% for carcasses. The isolati...
Source: Revista Argentina de Microbiologia - June 27, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Identification and antibiotic susceptibility of viridans group streptococci isolates recovered from patients hospitalized at a teaching hospital in Buenos Aires City].
Authors: Heine AC, García S, Barberis C, Vay C, E Mollerach M, Bonofiglio L, Famiglietti Á Abstract Members of the viridans group streptococci (VGS) are the cause of local and invasive infections. Due to the severity of these infections and taking into account that reports regarding epidemiological aspects are scarce, the aims of this work were the identification and the study of the antibiotic susceptibility profiles of the isolates recovered from patients that were hospitalized in order to find out about the resistance level and the epidemiology of infections in which VGS are involved. A hundred and...
Source: Revista Argentina de Microbiologia - June 27, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Responses of the common bean (Phaseolus vulgaris L.) and Rhizobium tropici CIAT899 symbiosystem to induced allelopathy by Ipomoea purpurea L. Roth].
Authors: Pérez-Peralta PJ, Ferrera-Cerrato R, Alarcón A, Trejo-Téllez LI, Cruz-Ortega R, Silva-Rojas HV Abstract Allelopathy is a phenomenon that involves the production of secondary metabolites that influence the growth of plants and microorganisms; however, this alellopathic effect has been scarcely studied on the rhizobia-legume symbiosis. The aims of this research were 1) to assess the allelopathic potential of aqueous extracts of Ipomoea purpurea L. Roth on seed germination and root length of common bean seedlings (Phaseolus vulgaris L.), 2) to determine its effects on the in vitro growth ...
Source: Revista Argentina de Microbiologia - June 13, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Development and removal ability of non-toxigenic Aspergillus section Flavi in presence of atrazine, chlorpyrifos and endosulfan.
This study evaluated the in vitro effect of three concentrations of atrazine, chlorpyrifos and endosulfan on the growth parameters of four non-toxigenic Aspergillus section Flavi strains. The ability of the strains to remove these pesticides in a synthetic medium was also determined. Growth parameters were measured on soil extract solid medium supplied with 5, 10 and 20mg/l of each pesticide, and conditioned to -0.70, -2.78, -7.06 and -10.0 water potential (MPa). Removal assays were performed in Czapek Doc medium (CZD) supplied with 20mg/l of each pesticide under optimal environmental conditions (-2.78 of MPa and 25°C)...
Source: Revista Argentina de Microbiologia - June 12, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

First report of Mycoplasma leachii isolation associated with disease in dairy calves in Argentina.
Authors: Neder VE, Allassia M, Amadio A, Calvinho LF Abstract There are few reports about the isolation of Mycoplasma species associated with cattle disease in Argentina. In this work we describe the detection of Mycoplasma leachii associated with disease in dairy calves in Santa Fe Province, Argentina. Samples obtained from a 4 day-old dairy calf suffering from polyarthritis and from two other calves, one with arthritis and the other one with a mandibular abscess, were subjected to microbiological culture. Classical culture and generic PCR confirmed the presence of Mycoplasma spp. The spacer region between the 16S...
Source: Revista Argentina de Microbiologia - June 3, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

In vitro comparison of acyclovir, ganciclovir and cidofovir against equid alphaherpesvirus 3 and evaluation of their efficacy against six field isolates.
Authors: Vissani MA, Zabal O, Tordoya MS, Parreño V, Thiry E, Barrandeguy M Abstract Equid alphaherpesvirus 3 (EHV3) is the etiological agent of equine coital exanthema (ECE), which is a venereal, highly contagious disease, characterized by the formation of papules, vesicles, pustules and ulcers on the external genitalia of mares and stallions. EHV3 remains in a latent state after a successful infection and there are latently infected animals in which the virus is reactivated and generally re-excreted subclinically. There are no available vaccines for this condition and prevention is based on the clinical ex...
Source: Revista Argentina de Microbiologia - May 23, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[The national scientific journals are in SciELO and more...]
Authors: Leotta GA PMID: 29739663 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - May 11, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Persistence, internalization and translocation of Escherichia coli O157:H7, O157:H16 and O105ab in plants and tomato fruits (Solanum lycopersicum L.)].
The objective of this study was to determine if three different strains of E. coli can penetrate and remain in plants and tomato fruits. A completely randomized experimental design was followed for which a tomato crop ("Cid" variety) was established under greenhouse conditions and three treatments were evaluated, T1 (E. coli O157:H7), T2 (E. coli from tomato cultivation [EcT] O157:H16), T3 (E. coli from spinach cultivation [EcH] O105ab) and a T4 control, with 100 plants each and four forms of inoculation: in the substrate, steam, petiole and the peduncle. Samples were carried out in vegetative stage, flowering, f...
Source: Revista Argentina de Microbiologia - May 3, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Molecular evidence that cellulolytic bacterial genus Cohnella is widespread among Neotropical Nasutitermitinae from NE Argentina.
Authors: Arneodo JD, Etcheverry C, Thebe T, Babalola OO, Godoy MC, Talia P Abstract Cohnella is a highly cellulolytic bacterial genus, which can be found in a variety of habitats. The aim of this study was to assess its presence in the digestive tract of termite species collected in North-eastern Argentina: Nasutitermes aquilinus, N. corniger and Cortaritermes fulviceps. Gut homogenates were incubated with cellulosic substrate for bacterial growth. Bacterial 16S rDNA was partially amplified using new primers for Cohnella spp. and cloned. Sequences obtained showed highest similarity (97.2-99.9%) with those of Cohnel...
Source: Revista Argentina de Microbiologia - April 28, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[The controversial Burkholderia cepacia complex, a group of plant growth promoting species and plant, animals and human pathogens].
Authors: Rojas-Rojas FU, López-Sánchez D, Meza-Radilla G, Méndez-Canarios A, Ibarra JA, Estrada-de Los Santos P Abstract The Burkholderia cepacia complex is a group of 22 species, which are known as opportunistic pathogens in immunocompromised people, especially those suffering from cystic fibrosis. It is also found in nosocomial infections and is difficult to eradicate due to intrinsic resistance to several antibiotics. The species have large genomes (up to 9 Mbp), distributed into 2-5 replicons. These features significantly contribute to genome plasticity, which makes them thrive in different...
Source: Revista Argentina de Microbiologia - April 26, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Cyanobacteria populations in rice soils: rotation effects].
Authors: Sánchez CI, Benintende MC, Benintende SM Abstract The aim of this study was to evaluate the effect of different rice rotations on cyanobacteria populations during the growing season. The study was conducted in Entre Ríos (Argentina). Four crop rotations were evaluated: rice-soybean (AS); rice-soybean-corn-soybean (ASMS); rice-rice (AA) and rice-pasture (AP). Soil and water samples were collected in 4 stages: implantation, tillering, booting and physiological maturity. The potential number of cyanobacteria population was lowerin relation to other rice sites previously reported. The lowest coun...
Source: Revista Argentina de Microbiologia - April 26, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Application of Quercus infectoria extract as a natural antimicrobial agent for chicken egg decontamination.
Authors: Tayel AA, El-Sedfy MA, Ibrahim AI, Moussa SH Abstract Egg contamination with microbial pathogens is an enduring worldwide concern. Natural products are frequently recommended as ideal alternatives to substitute synthetic and chemical antimicrobials. Oak galls (Quercus infectoria) are aberrant growths on oak trees that have many medicinal and pharmaceutical applications. Q. infectoria extract (QIE) antimicrobial action was assessed against many microbial species, and used for eggshell decontamination. QIE antimicrobial activity was evidenced against Staphylococcus aureus, Escherichia coli, Pseudomonas aerug...
Source: Revista Argentina de Microbiologia - April 26, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Synthesis and regulation of flavor compounds derived from brewing yeast: Esters].
This article focuses on esters, responsible for the floral and fruity beer flavor. Its formation depends on various enzymes and factors such as the concentration of wort nutrients, the amount of dissolved oxygen and carbon dioxide, fermentation temperature and mainly the genetics of the yeast used. We provide information about how the esters originate and how is the impact of different fermentative parameters on the final concentrations of these compounds and the quality of the end product. PMID: 29627148 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - April 10, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Isolation of Aspergillus tritici from internal environment (Chile): Ecological and clinical scope].
Authors: Vieille Oyarzo P, Cruz Choappa R, Piontelli Laforet E Abstract Indoor environments provide important protective habitats for humans, who live or work in them most of the time. Many of these environments lack ventilation, which affects the composition of microbial communities, especially that of the fungal community. The aim of this study is to report the isolation of Aspergillus section Candidi from indoor environments of the School of Medicine at Universidad de Valparaiso, Chile, and identification through morpho-physiological and molecular approaches. Their ecological and clinical features were highlight...
Source: Revista Argentina de Microbiologia - April 4, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Monitoring the degradation capability of novel haloalkaliphilic tributyltin chloride (TBTCl) resistant bacteria from butyltin-polluted site.
Authors: Hassan HA, Dawah SE, El-Sheekh MM Abstract Tributyltin (TBT) is recognized as a major environmental problem at a global scale. Haloalkaliphilic tributyltin (TBT)-degrading bacteria may be a key factor in the remediation of TBT polluted sites. In this work, three haloalkaliphilic bacteria strains were isolated from a TBT-contaminated site in the Mediterranean Sea. After analysis of the 16S rRNA gene sequences the isolates were identified as Sphingobium sp. HS1, Stenotrophomonas chelatiphaga HS2 and Rhizobium borbori HS5. The optimal growth conditions for biodegradation of TBT by the three strains were pH 9 ...
Source: Revista Argentina de Microbiologia - April 3, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Usefulness of rapid urease test samples for molecular analysis of clarithromycin resistance in Helicobacter pylori.
In conclusion, the biopsy specimens used for the rapid urease test were suitable samples for clarithromycin resistance detection in patients infected with H. pylori, which became especially useful in cases where the number or size of the biopsies is limited. In addition, this is the first report of a molecular analysis for clarithromycin resistance performed directly from gastric biopsies in our region. PMID: 29602600 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - April 1, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Usefulness of local health reports to link the incidence rate of diarrhea with the quality of drinking water].
In this study, we analyzed the reports of the health care center located in Vaqueros (Salta, Argentina) over an 8-month period. Moreover, we determined the concentration of Escherichia coli and Giardia spp. cysts in samples from four different drinking water sources. A statistical relationship between water quality and cases of diarrhea could not be found. However, using an odds ratio calculation, it was possible to determine that one of the studied drinking water systems acts as a protection factor in cases of diarrhea. The present work provides useful information for planning preventive measures by the local health syste...
Source: Revista Argentina de Microbiologia - March 25, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Development and storage studies of high density macrocapsules containing Lactobacillus spp. strains as nutritional supplement in young calves.
Authors: Astesana DM, Zimmermann JA, Frizzo LS, Zbrun MV, Blajman JE, Berisvil AP, Romero-Scharpen A, Signorini ML, Rosmini MR, Soto LP Abstract The aim of this study was to evaluate different production methodologies of probiotic macrocapsules with high bacterial densities destined to lactating calves. Three types of capsules containing Lactobacillus casei DSPV318T and Lactobacillus plantarum DSPV354T were prepared from an overnight culture in whey medium: (1) mixing the culture with calcium alginate and then, reincubating the capsules in whey (RC); (2) concentrating the biomass by centrifugation and mixing the pe...
Source: Revista Argentina de Microbiologia - March 24, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Prevalence of Trichomonas vaginalis and Human papillomavirus in female sex workers in Central Veracruz, Mexico.
The objective of this study was to determine the prevalence of T. vaginalis and HPV infections in FSWs in the city of Orizaba, Veracruz, Mexico. Samples from 105 FSWs were obtained by cervical swab and analyzed. The identification of T. vaginalis and HPV was performed by molecular methods. HPV DNA was identified in 5.71% of the samples with the presence of HPV16, HPV18, and HPV58. A percentage of 25.7% samples were positive for T. vaginalis for optical microscopy and 23.8% for PCR. The results of the study indicate the need to incorporate more sensitive methods for the timely diagnosis of STIs as well as comprehensive heal...
Source: Revista Argentina de Microbiologia - March 21, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Susceptibility to β-lactams in β-hemolytic streptococci.
Susceptibility to β-lactams in β-hemolytic streptococci. Rev Argent Microbiol. 2018 Mar 13;: Authors: Bonofiglio L, Gagetti P, García Gabarrot G, Kaufman S, Mollerach M, Toresani I, Vigliarolo L, von Specht M, Lopardo HA Abstract Group A (GAS), B (GBS), C (GCS) and G (GGS) β-hemolytic streptococci are important human pathogens. They cause infections of different severity and frequency. Nowadays, after 70 years of use, penicillin is still universally active against GAS, GCS and GGS. However, therapeutic failures have been recorded in 2-28% of pharyngitis cases (median: 12%) attribu...
Source: Revista Argentina de Microbiologia - March 21, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Carbapenemases in Acinetobacter baumannii. Review of their dissemination in Latin America.
Authors: Rodríguez CH, Nastro M, Famiglietti A Abstract Carbapenem resistance in gram-negative bacteria by production of carbapenemases is one of the most challenging issues regarding healthcare worldwide. We review the epidemiology and prevalence of carbapenemases in carbapenem-resistant Acinetobacter baumannii isolates from Latin American countries. High resistance rates to antimicrobial agents, particularly to carbapenems, are observed in this region. OXA-23 is the most widely disseminated class D-carbapenemase; it is present in all the countries of the region and is frequently associated to endemic clone...
Source: Revista Argentina de Microbiologia - March 21, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Characterization of extraintestinal pathogenic Escherichia coli strains isolated from household dogs and cats in Buenos Aires, Argentina].
In this study we characterized 85 extraintestinal pathogenic isolates genetically isolated from canines and felines. We used the Clermont scheme that includes intestinal (A and B1) and extraintestinal (B2 and D) phylogroups, virulence markers (pap1-2, pap3-4, sfa, afa, hlyA, aer and cnf) and hybrid pathogens. A percentage of 69.4% of the isolates belonged to phylogroup A; 1.2% to phylogroup B1; 16.5% to phylogroup B2 and 12.9% to phylogroup D. The most commonly found gene was sfa (21/85), followed by pap1-2 and cnf (20/85) and pap3-4 (19/85). No hybrids were detected. Animal isolates should be studied due to the zoonotic p...
Source: Revista Argentina de Microbiologia - March 21, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Scientific communication and the social practice of writing].
Authors: Zawoznik M PMID: 29519325 [PubMed - in process] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - March 11, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Demodex genus: colonizing parasites of healthy people or mites associated with ocular pathology?]
Authors: Mongi F, Laconte L, Casero RD Abstract Demodex folliculorum and Demodex brevis are mites that are responsible for human demodicosis affecting skin and frequently linked to ocular diseasess. The aim of this work was to determine the association between Demodex sp. and eyelid and eyelash diseases and to establish the predisposing factors for acquiring this parasitic disease. Eyelashes from symptomatic and asymptomatic patients (n=72) between 9 and 82 years old were analyzed, where 53% were positive for Demodex sp., of which 66% manifested ocular symptoms and pathologies, blepharitis and dry eye being prevale...
Source: Revista Argentina de Microbiologia - February 12, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Comparison of the identification results of Candida species obtained by BD Phoenix ™ and Maldi-TOF (Bruker Microflex LT Biotyper 3.1).
Comparison of the identification results of Candida species obtained by BD Phoenix™ and Maldi-TOF (Bruker Microflex LT Biotyper 3.1). Rev Argent Microbiol. 2018 Feb 05;: Authors: Marucco AP, Minervini P, Snitman GV, Sorge A, Guelfand LI, Moral LL, Integrantes de la Red de Micología CABA Abstract In patients with invasive fungal infections, the accurate and rapid identification of the genus Candida is of utmost importance since antimycotic sensitivity is closely related to the species. The aim of the present study was to compare the identification results of species of the genus Candida obt...
Source: Revista Argentina de Microbiologia - February 10, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Effectiveness of Beauveria bassiana (Baubassil ®) on the common cattle tick Rhipicephalus microplus in the Department of Guajira, Colombia].
[Effectiveness of Beauveria bassiana (Baubassil®) on the common cattle tick Rhipicephalus microplus in the Department of Guajira, Colombia]. Rev Argent Microbiol. 2018 Feb 02;: Authors: Tofiño Rivera AP, Ortega Cuadros M, Pedraza Claros B, Perdomo Ayola SC, Moya Romero DC Abstract The effectiveness of Baubassil®(Beauveria bassiana) on the tick Rhipicephalus (Boophilus) microplus was evaluated. In the in vitro phase, the optimal germination temperature of the fungus was determined by microcultures at 25, 30 and 35°C and the mortality of ticks. In the field, in a randomized design, 30 ...
Source: Revista Argentina de Microbiologia - February 8, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Detection of Chlamydia abortus in bovine reproductive losses in the province of La Pampa, Argentina].
This study shows the first detection of Chlamydiaceae and C. abortus DNA on reproductive losses of bovine cattle in Argentina, and the described prevalence value (4.78%) should be taken as baseline value due to the type of samples analyzed. Detection of genetic material from Chlamydiaceae not matching any of the studied species could be due to intraspecies variants or local species not yet described. Further research on Chlamydia infections in bovine cattle in Argentina is imperative to describe their range, to analyze their economic and zoonotic implications and to make recommendations about prevention and control measure...
Source: Revista Argentina de Microbiologia - January 21, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Non reported spa types in our country in Staphylococcus aureus from adult patients of a school hospital, Santa Fe - Argentina].
Authors: Tomatis C, Baroni MR, Mendosa MA, Nagel A, Mollerach A, Alvarez C, Zurbriggen ML, Cristobal S, Segovia G, Méndez ELA Abstract Staphylococcus aureus is a pathogen associated a different kind of infection. Molecular markers are useful tools to study microbial epidemiology. Twenty two methicillin-resistant S. aureus (MRSA) and 23 methicillin-susceptible S. aureus (MSSA) were studied by mecA gene, SCCmec cassette, Panton Valentine leucocidin (PVL) and spa polymorphism. The clinical data patients were analyzed. MSSA was prevalent in samples different from skin and soft tissue (SST) and in hospitalized pa...
Source: Revista Argentina de Microbiologia - January 19, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Bioconversion of hemicellulosic materials into ethanol by yeast, Pichia kudriavzevii 2-KLP1, isolated from industrial waste.
Authors: Elahi A, Rehman A Abstract In the present work, a yeast strain Pichia kudriavzevii was identified on the basis of 18S rDNA, showing maximum growth at 30°C and pH 7.0. Among all the complex polysaccharides used, wheat bran proved to be the best substrate as indicated by the maximum growth of the yeast strain. The yeast isolate was capable of producing xylanase both intra- and extra-cellularly, the dominant form being extracellular. The maximum enzyme activity was determined at pH 5.0 and at 50°C. Na+, Mg2+ and Fe2+ presence caused a substantial increase in enzyme activity while a slight decrease (4....
Source: Revista Argentina de Microbiologia - January 19, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Detection and characterization of Shiga toxin-producing Escherichia coli in children treated at an inter-zonal pediatric hospital in the city of La Plata].
This study shows that STEC of different serotypes and genotypes circulate in the city of La Plata and surroundings. Despite the genetic diversity observed between the O157:H7 isolates, some were indistinguishable by the subtyping techniques used. PMID: 29336911 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - January 19, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research