Genetic diversity of Mycoplasma hyopneumoniae in Mendoza province.
Authors: Sosa C, Blois A, Ibáñez F, Tamiozzo P Abstract In Argentina, enzootic pneumonia (EP) is highly prevalent and different genetic types of Mycoplasma hyopneumoniae have been identified. However, there is a lack of information about prevalence and other epidemiological aspects of EP in Mendoza province. A multiple Locus variable-number tandem repeat analysis (MLVA) targeting P97 R1, P97 R1A and P146 R3 loci was used to assess the genetic diversity of M. hyopneumoniae from clinical specimens recovered from pigs from five herds located in different districts of Mendoza province. M. hyopneumoniae co...
Source: Revista Argentina de Microbiologia - January 19, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Yeast diversity in Vitis non-vinifera ecosystems.
Authors: Raymond Eder ML, Rosa AL Abstract The surface of grapes lodges a complex community of yeast species responsible for spontaneous alcoholic fermentation. The study of indigenous Saccharomyces and "non-Saccharomyces" yeasts during grape must fermentation constitutes a major research area in microbial enology. Although there are detailed studies on the microbiota of Vitis vinifera L. grapes, little is known about the diversity of yeast communities present in non-vinifera Vitis ecosystems (i.e., grapes and spontaneously fermenting grape musts). Potentially scientific and/or enological valuable yeast s...
Source: Revista Argentina de Microbiologia - January 16, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Detection of serovars of Salmonella in artificially reared calves in Mar y Sierras Dairy Basin, Argentina].
In conclusion, 11 Salmonella serovars were detected in one out of 3dairy farms in Mar and Sierras Dairy Basin, and not only were these serovars associated with diarrhea signs including the presence of mucus in feces, but they were also more prevalent among calves aged up to 21 days. PMID: 30638637 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - January 16, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Clinical and microbiological characteristics of community-acquired pneumonia associated with Streptococcus pneumoniae in adult patients in Mexico.
In conclusion, head trauma was the most common comorbidity in the CAP group. The most prevalent serotype was 19A. Decreased susceptibility to most antimicrobials tested was observed. PMID: 30635204 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - January 14, 2019 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Electronic cardiovascular device-associated to infective endocarditis caused by Mycobacterium mageritense].
We describe a case of endocarditis caused by Mycobacterium mageritense in a patient with an autologous pericardial patch and a pressure catheter in the left auricle. The bacterial species was identified as Mycobacterium mageritense by mass spectrometry (MALDI-TOF MS), score 2.3, and confirmed by 16S rRNA analysis with 99.8 and 100% agreement, respectively. PMID: 30591316 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - December 30, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

National production of certified reference fungal cultures.
Authors: Davel GO, Mazza M, Refojo N, Taverna CG, Rivas MC, Fernández J, Vivot ME, Mazza MG, Mastromónaco GM, Bueno NS, Molina V, Brero ML, Kornblit F Abstract Reference fungal cultures (RFCs) are essential for the internal quality control of laboratories. The production of these cultures requires standardized procedures (IRAM 14950:2016 and ISO 17034:2016 standards) carried out by a recognized and accredited laboratory. The aim of this work was to produce RFC in paper disks of autochthonous strains, characterized by two, homogeneous and stable reference methods traceable at species level. RFC were pr...
Source: Revista Argentina de Microbiologia - December 30, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Species diversity of non-tuberculous mycobacteria isolated from  aquatic environments of General Pico city, Province of La Pampa (Argentina)].
[Species diversity of non-tuberculous mycobacteria isolated from aquatic environments of General Pico city, Province of La Pampa (Argentina)]. Rev Argent Microbiol. 2018 Dec 19;: Authors: Tortone CA, Oriani DS, Staskevich AS, Oriani AS, Gino LM, Marfil MJ, Nava Vargas A, Gioffré AK, Zumárraga MJ Abstract Non-tuberculous mycobacteria (NTM) are studied not only for their importance as emerging opportunistic pathogens but also for their applications in biotechnology and bioremediation. Our aim was to determine the occurrence and diversity of mycobacteria in different aquatic habitats of...
Source: Revista Argentina de Microbiologia - December 25, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Zoonosis and veterinary waste disposal in rural practice.
The objectives of this study were to estimate: (a) the frequency of zoonoses in large animal veterinarians from rural areas of the province of Buenos Aires, Argentina, and (b) to describe the use and disposal of personal protective equipment (PPE) and selective veterinary clinical waste. A cross-sectional study was carried out on large animal veterinary practitioners in the Province of Buenos Aires (n=106). One third (29.2%) of them had been diagnosed with a zoonosis by laboratory-methods, being brucellosis the most frequent (22.6%). The more years passed since their graduation, the greater the chances of becoming ill (p
Source: Revista Argentina de Microbiologia - December 20, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Solid state fermentation to obtain vegetable products bio-enriched with isoflavone aglycones using lactic cultures.
Authors: Correa Deza MA, Rodríguez de Olmos A, Garro MS Abstract The consumption of soybean isoflavones (IS) is associated with several beneficial properties on human health. Some lactic acid bacteria possess β-glucosidase enzyme, that allows to obtain the active form of IS (aglycone). The solid state fermentation (SSF) has received great attention in the last years in order to obtain several valuable compounds. SSF, using soybean as substrate and Lactobacillus rhamnosus CRL 981 as starter, was studied in the present work. Sucrose was added into soybean paste to study the effect on the behavior of the s...
Source: Revista Argentina de Microbiologia - December 20, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Development and evaluation of an in-house database for quick identification of Burkholderia contaminans by MALDI-TOF MS].
Authors: Cipolla L, Rocca F, Armitano RI, Martinez C, Almuzara M, Faccone D, Vay C, Prieto M Abstract MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry (MS) proved to be a robust tool for the identification of numerous taxonomic groups. However, it has limitations. A key advantage of this technique is the flexibility for the incorporation of protein profiles of microorganisms not included in the commercial database. Due to the prevalence of Burkholderia contaminans in fibrocystic patients in Argentina and the fact that rapid and reliable microbiological diagnosis is crucial in...
Source: Revista Argentina de Microbiologia - December 20, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Molecular epidemiology of carbapenem-resistant isolates of Acinetobacter baumannii in Argentina].
Authors: H Rodríguez C, Nastro M, A Flores S, Rodriguez M, Spinozzi M, Bruni G, L López A, David V, S Aiassa M, A Marqués I, R Navarro O, Paniccia L, Famiglietti A, Grupo colaborativo Acinetobacter Argentina Abstract One hundred sequential, epidemiologically unrelated carbapenem-resistant- Acinetobacter baumannii isolates from 11 hospitals in 10 Argentine provinces were collected between January and August 2016. Genes coding for Ambler class D and B carbapenemases were investigated by PCR using specific primers. All isolates were typed using the 3-locus sequence typing and blaOXA-51-like sequen...
Source: Revista Argentina de Microbiologia - December 17, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Identification of Brucella abortus biovar 4 of bovine origin in Colombia.
The objective of this study was to identify twelve Brucella abortus isolates of bovine origin from the department of Nariño in Colombia up to the biovar level. These isolates are included in the collection of the Germplasm Bank of Microorganisms of Animal Health Interest - Bacteria and Virus (BGSA-BV). The identification was carried out through conventional methods such as macro and microscopic morphological descriptions, enzymatic activity, biochemical profile, substrate use and sensitivity to dyes. Complementary genotypic characterization was carried out using multiplex PCR for B. abortus, Brucella melitensis, Bru...
Source: Revista Argentina de Microbiologia - December 17, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Microbiological analysis of the maternal genital tract and umbilical cord blood and its association with neonatal damage].
The objective of the study was to identify microorganisms of the maternal genital tract that are associated with neonatal damage, in order to prevent future perinatal complications. Seven hundred and eleven pregnant patients attended their prenatal control during the period January 2010-July 2013. Ureaplasma urealyticum and Mycoplasma hominis presence was investigated in umbilical cord blood by metabolic substrates (Micofast-Biomerieux) and that of T.vaginalis, by PCR using specific primers. The microbiological study of the vaginal contents of 288 pregnant patients at weeks 35 to 37 was performed by conventional methods, a...
Source: Revista Argentina de Microbiologia - December 15, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Predatory journals: An evil of this time?]
Authors: Sauka DH PMID: 30482270 [PubMed - in process] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - December 3, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Diversity and abundance of denitrifiers during cow manure composting.
Authors: Meng Q, Xu X, Zhang W, Cheng L, Men M, Xu B, Deng L, Sun X Abstract Diversity and abundance of the denitrifying genes nirK, nirS and nosZ were investigated in cow manure compost using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and real-time quantitative PCR (qPCR), respectively. These three genes were detected in all the stages of the composting process. Phylogenetic analysis showed that the nirK gene was closely related to Rhizobiales, Burkholderiales, the nirS gene was closely related to Pseudomonadales and Burkholderiales, and the nosZ gene was closely related to Rhodos...
Source: Revista Argentina de Microbiologia - November 24, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Immunoprotective response induced by recombinant glycoprotein D in the BALB/c respiratory mouse model of Equid alphaherpesvirus 1 infection.
Authors: Fuentealba NA, Sguazza GH, Zanuzzi CN, Bravi ME, Scrochi MR, Valera AR, Corva SG, Gimeno EJ, Pecoraro MR, Galosi CM Abstract Equid alphaherpesvirus 1 (EHV-1) infection causes abortion, respiratory disease, perinatal deaths and neurological disorders in horses. The natural infection and available vaccines provide only partial and short-lived protection against reinfections. In the present study, we analyzed the ability of purified baculovirus-expressed glycoprotein D (gD) administered by different routes to induce protective immunity in BALB/c mice after challenge with the EHV-1 AR8 strain. Clinical signs v...
Source: Revista Argentina de Microbiologia - November 4, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Histoplasma capsulatum electron microscopy isolated from a patient with disseminated histoplasmosis].
Authors: Pedersen D, Fiore C, Arechavala A, Paniccia L, Occhionero M PMID: 30377014 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - November 1, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Isolation and characterization of a marine testosterone-degrading bacterium: Vibrio sp. N3.
In this study, a marine testosterone-degrading bacterium (strain N3) was isolated from Nanao Island in the South China Sea. In addition, the strain could also use 17β-estradiol (E2), 17β-ethinyl estradiol (EE2), estriol (E3) or cholesterol as a sole carbon source. According to the 16S rRNA gene sequence analysis, strain N3 was identified as Vibrio sp. Further characterization showed that the strain is aerobic, gram-negative, and mobile and exhibits resistance to ampicillin, carbenicillin, penicillin and spectinomycin. For enhancing its capacity of testosterone degradation, the Plackett-Burman factorial design and...
Source: Revista Argentina de Microbiologia - October 11, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Atypical hand, foot, and mouth disease caused by Coxsackievirus A6 in Argentina in 2015.
We present two groups of cases of atypical hand, foot, and mouth disease (HFMD) caused by Coxsackievirus A6 (CV-A6) detected in Argentina in 2015. The first group involved 14 patients from Chubut province and the second group affected 12 patients from San Luis province. Molecular analysis of the complete VP1 protein gene revealed the circulation of E2 sublineage, the most predominant worldwide. To our knowledge, this is the first report of CV-A6 infections associated with atypical HFMD in Argentina and South America. PMID: 30269935 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - October 4, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Clinical and epidemiological characteristics of patients with paracoccidioidomycosis diagnosed in a hospital of Resistencia, Chaco].
Authors: Tracogna MF, Fernández Lugo S, Gariboglio Vázquez ML, Fernández MS, Andriani ME, Presti SE, Arce V, López R, Iliovich E, Marques IA, Cattana ME Abstract In order to describe the clinical and epidemiological characteristics of paracoccidioidomycosis, a descriptive study of all the cases diagnosed by the Clinical Microbiology Service at Dr. Julio C. Perrando hospital in the city of Resistencia (Chaco Province, Argentina) was conducted. Between 2011 and 2014, 46 cases were detected. In the period 2013-2014, an almost 4-fold increase in the incidence rate was detected. The chronic f...
Source: Revista Argentina de Microbiologia - September 25, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Resistance to β-lactams in enterococci.
Resistance to β-lactams in enterococci. Rev Argent Microbiol. 2018 Sep 19;: Authors: Gagetti P, Bonofiglio L, García Gabarrot G, Kaufman S, Mollerach M, Vigliarolo L, von Specht M, Toresani I, Lopardo HA Abstract Enterococci are intrinsically resistant to several antimicrobial classes and show a great ability to acquire new mechanisms of resistance. Resistance to β-lactam antibiotics is a major concern because these drugs either alone or in combination are commonly used for the treatment of enterococcal infections. Ampicillin resistance, which is rare in Enterococcus faecalis, occurs i...
Source: Revista Argentina de Microbiologia - September 25, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Observation of interactions between hyphae of Fusarium verticillioides, Aspergillus flavus and Talaromyces funiculosus in microcultures developed in hermetic environments].
Authors: Castellari CC, Marcos Valle FJ, Pacin AM PMID: 30243526 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - September 25, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Helicobacter cinaedi bacteremia: Presentation of the first cases reported in Argentina].
In this report we present the first documented cases of bacteremia caused by H. cinaedi in Argentina. PMID: 30243527 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - September 25, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Acute disseminated encephalomyelitis due to Mycoplasma pneumoniae in a previously healthy boy].
We present here the case of a previously healthy 5 year-old boy hospitalized in an intensive care unit due to tonic-clonic seizures focused on the face and right side of the body, and axillary temperature of 37.4°C. Common bacterial and viral etiology was ruled out through studies of cerebrospinal fluid (CSF) samples. Mycoplasma pneumoniae was suspected by a positive immunofluorescence serum test for IgM class antibodies. Finally, with a brain biopsy, M. pneumoniae was confirmed by polymerase chain reaction (PCR) and acute disseminated encephalomyelitis by pathological anatomy. The patient was treated with clarithromyc...
Source: Revista Argentina de Microbiologia - September 22, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Bacterial diversity in the Cr(VI) reducing biocathode of a Microbial Fuel Cell with salt bridge.
In this study, an efficient biocathode for the reduction of Cr(VI) with future use in bioremediation, was characterized. PMID: 30144991 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - August 28, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Antifungal and antimycotoxigenic effect of Lactobacillus plantarum CRL 778 at different water activity values.
In this study, the ability of Lactobacillus (L.) plantarum CRL 778 to control growth and OTA production by Aspergillus (A.) niger 13D strain, at different water activity (aw) values (0.955, 0.964, 0.971, 0.982, and 0.995) was determined in vitro. Both parameters were significantly (p
Source: Revista Argentina de Microbiologia - August 28, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Infective endocarditis due to non-HACEK gram-negative bacilli in a Level III cardiovascular center in Argentina (1998-2016)].
Authors: Burgos LM, Oses P, Iribarren AC, Pennini M, Merkt M, Vrancic M, Camporrotondo M, Ronderos R, Sucari A, Nacinovich F Abstract Non-HACEK Gram-negative bacilli are a rare cause of infective endocarditis. Epidemiological, diagnostic and prognostic aspects of this entity are little known, and there is limited experience. The aim of this study was to analyze the clinical, microbiological and in-hospital outcomes of non-HACEK Gram negative bacilli endocarditis and to compare them with those due to other microorganisms. PMID: 30143351 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - August 26, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Persist and triumph: persistent cells in microbial biofilm].
Authors: Paraje MG PMID: 30057356 [PubMed - in process] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - August 1, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Growth inhibition and genetic modification of Phytophthora capsici using chitosan with low degree of polymerization].
Authors: Ramírez-Benítez JE, Arjona Sabido RA, Caamal Velázquez JH, Rodríguez Ávila NL, Solís Pereira SE, Lizama Uc G Abstract Phytophthora blight of peppers, caused by oomycete Phytophthora capsici, currently causes economic losses in crops such as peppers, tomatoes, eggplant and cucurbits. In this work, we evaluated the effect of chitosan with low degree of polymerization (LDP) on growth and gene expression of P. capsici cultures. LDP chitosan inhibited 88% of P. capsici mycelial growth at concentrations up to 0,4 mg/l, whereas at concentrations higher than 1,6 mg/l it co...
Source: Revista Argentina de Microbiologia - July 26, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Silver nanoparticles from Justicia spicigera and their antimicrobial potentialities in the biocontrol of foodborne bacteria and phytopathogenic fungi.
Authors: Bernardo-Mazariegos E, Valdez-Salas B, González-Mendoza D, Abdelmoteleb A, Tzintzun Camacho O, Ceceña Duran C, Gutiérrez-Miceli F Abstract In the present work, the biosynthesis of silver-nanoparticles (AgNP) was evaluated using the aqueous extract from Justicia spicigera. The obtained silver nanoparticles were characterized using UV-visible spectroscopic techniques, energy dispersive X-ray spectrometers (EDS), zeta potential and dynamic light scattering. The antimicrobial activity of biosynthesized AgNP was tested against foodborne bacteria (Bacillus cereus, Klebsiella pneumoniae and E...
Source: Revista Argentina de Microbiologia - July 23, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Detection and molecular characterization of Chlamydia psittaci and Chlamydia abortus in psittacine pet birds in Buenos Aires province, Argentina.
This report contributes to the improvement of our understanding of chlamydial agents in our country. PMID: 30017323 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - July 20, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Veil-like pellicle development by Azospirillum brasilense in semisolid NFb medium.
Authors: Di Salvo LP, García de Salamone IE PMID: 30017324 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - July 20, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

The wide distribution of an extremely thermoacidophilic microorganism in the copper mine at ambient temperature and under acidic condition and its significance in bioleaching of a chalcopyrite concentrate.
In this study samples were collected at ambient temperature from Sarcheshmeh Copper Complex in Iran. Mixed samples were inoculated into the culture medium for enrichment of the microorganisms. Pure cultures from these enrichments were obtained by subculture of liquid culture to solid media. Morphological observation was performed under the scanning electron microscope. Isolates were adapted to 30% (w/v) pulp density. For the bioleaching test, the experiments were designed with DX7 software. Bioleaching experiments were carried out in Erlenmeyer flasks and a stirred tank reactor. The highest copper recovery in Erlenmeyer fl...
Source: Revista Argentina de Microbiologia - June 30, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Current scenario of plasmid-mediated colistin resistance in Latin America.
Authors: Quiroga C, Nastro M, Di Conza J Abstract Colistin resistance can occur by chromosomal mutations and by acquisition of plasmid-carrying determinants, mainly mcr-1. In the recent years, we have observed the outburst of this resistance gene in our region. Due to the risk of the rapid dissemination of mcr-1, this finding has worried and alerted different actors from the health field and has become one of the most prolific topics. Our review compiles available reports of well-documented mcr-1-positive strains of Enterobacteriaceae, obtained from different samples in Argentina and other countries of Latin Americ...
Source: Revista Argentina de Microbiologia - June 29, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Frequency, characterization and genotypic analysis of Shiga toxin-producing Escherichia coli in beef slaughterhouses of Argentina.
The objectives of this study were: (1) to estimate STEC frequency in hide and carcass samples taken from beef slaughterhouses supplying the domestic market in Argentina, (2) to establish the pheno-genotypic characteristics of STEC and non-toxigenic Escherichia coli of serogroups O26, O45, O103, O121, O111, O145 or O157 isolated from the analyzed samples and, (3) to study their clonal relatedness. Sixty hides and 60 carcasses were analyzed. At the screening step, 48% of hide and 80% of carcass samples tested positive for the stx gene by endpoint PCR. The STEC isolation rate was 5% for hides and 8% for carcasses. The isolati...
Source: Revista Argentina de Microbiologia - June 27, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Identification and antibiotic susceptibility of viridans group streptococci isolates recovered from patients hospitalized at a teaching hospital in Buenos Aires City].
Authors: Heine AC, García S, Barberis C, Vay C, E Mollerach M, Bonofiglio L, Famiglietti Á Abstract Members of the viridans group streptococci (VGS) are the cause of local and invasive infections. Due to the severity of these infections and taking into account that reports regarding epidemiological aspects are scarce, the aims of this work were the identification and the study of the antibiotic susceptibility profiles of the isolates recovered from patients that were hospitalized in order to find out about the resistance level and the epidemiology of infections in which VGS are involved. A hundred and...
Source: Revista Argentina de Microbiologia - June 27, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Responses of the common bean (Phaseolus vulgaris L.) and Rhizobium tropici CIAT899 symbiosystem to induced allelopathy by Ipomoea purpurea L. Roth].
Authors: Pérez-Peralta PJ, Ferrera-Cerrato R, Alarcón A, Trejo-Téllez LI, Cruz-Ortega R, Silva-Rojas HV Abstract Allelopathy is a phenomenon that involves the production of secondary metabolites that influence the growth of plants and microorganisms; however, this alellopathic effect has been scarcely studied on the rhizobia-legume symbiosis. The aims of this research were 1) to assess the allelopathic potential of aqueous extracts of Ipomoea purpurea L. Roth on seed germination and root length of common bean seedlings (Phaseolus vulgaris L.), 2) to determine its effects on the in vitro growth ...
Source: Revista Argentina de Microbiologia - June 13, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Development and removal ability of non-toxigenic Aspergillus section Flavi in presence of atrazine, chlorpyrifos and endosulfan.
This study evaluated the in vitro effect of three concentrations of atrazine, chlorpyrifos and endosulfan on the growth parameters of four non-toxigenic Aspergillus section Flavi strains. The ability of the strains to remove these pesticides in a synthetic medium was also determined. Growth parameters were measured on soil extract solid medium supplied with 5, 10 and 20mg/l of each pesticide, and conditioned to -0.70, -2.78, -7.06 and -10.0 water potential (MPa). Removal assays were performed in Czapek Doc medium (CZD) supplied with 20mg/l of each pesticide under optimal environmental conditions (-2.78 of MPa and 25°C)...
Source: Revista Argentina de Microbiologia - June 12, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

First report of Mycoplasma leachii isolation associated with disease in dairy calves in Argentina.
Authors: Neder VE, Allassia M, Amadio A, Calvinho LF Abstract There are few reports about the isolation of Mycoplasma species associated with cattle disease in Argentina. In this work we describe the detection of Mycoplasma leachii associated with disease in dairy calves in Santa Fe Province, Argentina. Samples obtained from a 4 day-old dairy calf suffering from polyarthritis and from two other calves, one with arthritis and the other one with a mandibular abscess, were subjected to microbiological culture. Classical culture and generic PCR confirmed the presence of Mycoplasma spp. The spacer region between the 16S...
Source: Revista Argentina de Microbiologia - June 3, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

In vitro comparison of acyclovir, ganciclovir and cidofovir against equid alphaherpesvirus 3 and evaluation of their efficacy against six field isolates.
Authors: Vissani MA, Zabal O, Tordoya MS, Parreño V, Thiry E, Barrandeguy M Abstract Equid alphaherpesvirus 3 (EHV3) is the etiological agent of equine coital exanthema (ECE), which is a venereal, highly contagious disease, characterized by the formation of papules, vesicles, pustules and ulcers on the external genitalia of mares and stallions. EHV3 remains in a latent state after a successful infection and there are latently infected animals in which the virus is reactivated and generally re-excreted subclinically. There are no available vaccines for this condition and prevention is based on the clinical ex...
Source: Revista Argentina de Microbiologia - May 23, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[The national scientific journals are in SciELO and more...]
Authors: Leotta GA PMID: 29739663 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - May 11, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Persistence, internalization and translocation of Escherichia coli O157:H7, O157:H16 and O105ab in plants and tomato fruits (Solanum lycopersicum L.)].
The objective of this study was to determine if three different strains of E. coli can penetrate and remain in plants and tomato fruits. A completely randomized experimental design was followed for which a tomato crop ("Cid" variety) was established under greenhouse conditions and three treatments were evaluated, T1 (E. coli O157:H7), T2 (E. coli from tomato cultivation [EcT] O157:H16), T3 (E. coli from spinach cultivation [EcH] O105ab) and a T4 control, with 100 plants each and four forms of inoculation: in the substrate, steam, petiole and the peduncle. Samples were carried out in vegetative stage, flowering, f...
Source: Revista Argentina de Microbiologia - May 3, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Molecular evidence that cellulolytic bacterial genus Cohnella is widespread among Neotropical Nasutitermitinae from NE Argentina.
Authors: Arneodo JD, Etcheverry C, Thebe T, Babalola OO, Godoy MC, Talia P Abstract Cohnella is a highly cellulolytic bacterial genus, which can be found in a variety of habitats. The aim of this study was to assess its presence in the digestive tract of termite species collected in North-eastern Argentina: Nasutitermes aquilinus, N. corniger and Cortaritermes fulviceps. Gut homogenates were incubated with cellulosic substrate for bacterial growth. Bacterial 16S rDNA was partially amplified using new primers for Cohnella spp. and cloned. Sequences obtained showed highest similarity (97.2-99.9%) with those of Cohnel...
Source: Revista Argentina de Microbiologia - April 28, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[The controversial Burkholderia cepacia complex, a group of plant growth promoting species and plant, animals and human pathogens].
Authors: Rojas-Rojas FU, López-Sánchez D, Meza-Radilla G, Méndez-Canarios A, Ibarra JA, Estrada-de Los Santos P Abstract The Burkholderia cepacia complex is a group of 22 species, which are known as opportunistic pathogens in immunocompromised people, especially those suffering from cystic fibrosis. It is also found in nosocomial infections and is difficult to eradicate due to intrinsic resistance to several antibiotics. The species have large genomes (up to 9 Mbp), distributed into 2-5 replicons. These features significantly contribute to genome plasticity, which makes them thrive in different...
Source: Revista Argentina de Microbiologia - April 26, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Cyanobacteria populations in rice soils: rotation effects].
Authors: Sánchez CI, Benintende MC, Benintende SM Abstract The aim of this study was to evaluate the effect of different rice rotations on cyanobacteria populations during the growing season. The study was conducted in Entre Ríos (Argentina). Four crop rotations were evaluated: rice-soybean (AS); rice-soybean-corn-soybean (ASMS); rice-rice (AA) and rice-pasture (AP). Soil and water samples were collected in 4 stages: implantation, tillering, booting and physiological maturity. The potential number of cyanobacteria population was lowerin relation to other rice sites previously reported. The lowest coun...
Source: Revista Argentina de Microbiologia - April 26, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Application of Quercus infectoria extract as a natural antimicrobial agent for chicken egg decontamination.
Authors: Tayel AA, El-Sedfy MA, Ibrahim AI, Moussa SH Abstract Egg contamination with microbial pathogens is an enduring worldwide concern. Natural products are frequently recommended as ideal alternatives to substitute synthetic and chemical antimicrobials. Oak galls (Quercus infectoria) are aberrant growths on oak trees that have many medicinal and pharmaceutical applications. Q. infectoria extract (QIE) antimicrobial action was assessed against many microbial species, and used for eggshell decontamination. QIE antimicrobial activity was evidenced against Staphylococcus aureus, Escherichia coli, Pseudomonas aerug...
Source: Revista Argentina de Microbiologia - April 26, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Synthesis and regulation of flavor compounds derived from brewing yeast: Esters].
This article focuses on esters, responsible for the floral and fruity beer flavor. Its formation depends on various enzymes and factors such as the concentration of wort nutrients, the amount of dissolved oxygen and carbon dioxide, fermentation temperature and mainly the genetics of the yeast used. We provide information about how the esters originate and how is the impact of different fermentative parameters on the final concentrations of these compounds and the quality of the end product. PMID: 29627148 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - April 10, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

[Isolation of Aspergillus tritici from internal environment (Chile): Ecological and clinical scope].
Authors: Vieille Oyarzo P, Cruz Choappa R, Piontelli Laforet E Abstract Indoor environments provide important protective habitats for humans, who live or work in them most of the time. Many of these environments lack ventilation, which affects the composition of microbial communities, especially that of the fungal community. The aim of this study is to report the isolation of Aspergillus section Candidi from indoor environments of the School of Medicine at Universidad de Valparaiso, Chile, and identification through morpho-physiological and molecular approaches. Their ecological and clinical features were highlight...
Source: Revista Argentina de Microbiologia - April 4, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Monitoring the degradation capability of novel haloalkaliphilic tributyltin chloride (TBTCl) resistant bacteria from butyltin-polluted site.
Authors: Hassan HA, Dawah SE, El-Sheekh MM Abstract Tributyltin (TBT) is recognized as a major environmental problem at a global scale. Haloalkaliphilic tributyltin (TBT)-degrading bacteria may be a key factor in the remediation of TBT polluted sites. In this work, three haloalkaliphilic bacteria strains were isolated from a TBT-contaminated site in the Mediterranean Sea. After analysis of the 16S rRNA gene sequences the isolates were identified as Sphingobium sp. HS1, Stenotrophomonas chelatiphaga HS2 and Rhizobium borbori HS5. The optimal growth conditions for biodegradation of TBT by the three strains were pH 9 ...
Source: Revista Argentina de Microbiologia - April 3, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

Usefulness of rapid urease test samples for molecular analysis of clarithromycin resistance in Helicobacter pylori.
In conclusion, the biopsy specimens used for the rapid urease test were suitable samples for clarithromycin resistance detection in patients infected with H. pylori, which became especially useful in cases where the number or size of the biopsies is limited. In addition, this is the first report of a molecular analysis for clarithromycin resistance performed directly from gastric biopsies in our region. PMID: 29602600 [PubMed - as supplied by publisher] (Source: Revista Argentina de Microbiologia)
Source: Revista Argentina de Microbiologia - April 1, 2018 Category: Microbiology Tags: Rev Argent Microbiol Source Type: research