Optimization in the detection of bacteremia: Integrated use of FilmArray (BCID2) and MALDI-TOF

Rev Argent Microbiol. 2023 Jul 18:S0325-7541(23)00052-4. doi: 10.1016/j.ram.2023.06.001. Online ahead of print.ABSTRACTThe usefulness of the combined use of MALDI-TOF MS from a subculture with 3-5h of incubation and the BCID2 panel (FilmArray) for the identification of microorganisms from positive blood cultures and its importance in the adjustment of antimicrobial therapy was analyzed. Overall identification with BCID2 was 90.4% (142/157) and with Maldi-TOF MS 83.4% (131/157) (p=0.0858); in 23 polymicrobial episodes (47 strains), the BCID2 panel identified 45 (95.7%) and MALDI-TOF MS 24 (51.1%) (p<0.0000). BCID2 detected the presence of the resistance genes mecA/C (n=16), blaKPC (n=8); blaCTX-M (n=17), blaNDM (n=8), blaOXA-48 (n=1), and vanA/B (n=2). The median time to report a result was 2.0h for BCID2 and 4.0h for MALDI-TOF MS (p<0.0000). Of 124 episodes analyzed, the rapid result of BCID2 led to 82.3% (102/124) therapeutic changes.PMID:37474389 | DOI:10.1016/j.ram.2023.06.001

Source: Revista Argentina de Microbiologia - July 20, 2023 Category: Microbiology Authors: Natalia A Carrion Rodolfo E Quiroz Camila Asenzo Manuela Gamarra Antonio E Flores Maria Garcia Rolando N Soloaga Julia Grigioni Maria Mastroianni Alejandra Margari Facundo Molina Adriana N Procopio Vanesa Reijtman Maria S Ratti Victor Mamani Myriam Vazque Source Type: research