Critical PDT theory: Viability, cytotoxicity, proliferation and clonogenicity —Is there a difference?
Comparison of MTT versus clonogenic effects of photodynamic therapy in MCF-7c3 breast cancer cells in culture. Left: MTT data; right clonogenic data. A collection of photosensitizers was used to produce these results. (Source: Photochemistry and Photobiology)
Source: Photochemistry and Photobiology - July 24, 2023 Category: Science Authors: David Kessel Tags: CORRESPONDENCE Source Type: research
Mutagenicity assessment of high ‐power 1.6‐THz pulse laser radiation
The present study aimed to assess the mutagenicity of 1.6 THz laser irradiation in the Ames test. The single bacterial colony of tester strains (TA98, TA100, TA1535, TA1537, and WP2uvrA) was irradiated with a 1.6 THz laser using a tabletop THz pulse laser system. The results showed that the THz laser irradiation did not increase the number of revertant colonies compared to that in the negative control group, whereas the positive control group treated with chemicals known as mutagens increased the number of revertant colonies. Thus, it is suggested that 1.6 THz laser irradiation is unlikely to be mutagenic. AbstractThe effe...
Source: Photochemistry and Photobiology - July 22, 2023 Category: Science Authors: Midori Shirato,
Yuma Takida,
Taro Kanno,
Hiroshi Matsuura,
Yoshimi Niwano,
Hiroaki Minamide,
Keisuke Nakamura Tags: RESEARCH ARTICLE Source Type: research
Rose bengal –encapsulated chitosan nanoparticles for the photodynamic treatment of Trichophyton species
Encapsulated rose bengal nanoparticles in combination with photodynamic therapy are highly effective for eradicating clinically relevantTrichophyton spores that cause onychomycosis. AbstractRose bengal (RB) solutions coupled with a green laser have proven to be efficient in clearing resilient nail infections caused byTrichophyton rubrum in a human pilot study and in extensive in vitro experiments. Nonetheless, the RB solution can become diluted or dispersed over the tissue and prevented from penetrating the nail plate to reach the subungual area where fungal infection proliferates. Nanoparticles carrying RB can mitigate th...
Source: Photochemistry and Photobiology - July 21, 2023 Category: Science Authors: Alina Bekmukhametova,
Anu Antony,
Catriona Halliday,
Sharon Chen,
Chun ‐Hoong Ho,
Mir Muhammad Nasir Uddin,
Leonardo Longo,
Christian Pedrinazzi,
Laurel George,
Richard Wuhrer,
Simon Myers,
Damia Mawad,
Jessica Houang,
Antonio Lauto Tags: RESEARCH ARTICLE Source Type: research
Preclinical evaluation of singlet oxygen ‐cleavable prodrugs in combination with protoporphyrin IX‐photodynamic therapy in an orthotopic rat model of non‐muscle‐invasive bladder cancer†
Compared to Photofrin PDT, PpIX-PDT showed less bladder toxicity but lacked efficacy. A singlet oxygen-cleavable prodrug was developed to improve PpIX efficacy in treating NMIBC. PpIX-PDT + prodrug combination group showed better efficacy than PpIX-PDT alone, when compared as fraction bladder area covered by tumors and tumor volume. Neither the PpIX-PDT alone nor the combination treatment caused apparent muscle damage. AbstractPhotodynamic therapy (PDT) initially employed red light, which caused some patients to experience permanent bladder contractions. PDT using the FDA-approved drug hexaminolevulinate (HAL), which ...
Source: Photochemistry and Photobiology - July 21, 2023 Category: Science Authors: Kazi Md Mahabubur Rahman,
Barbara A. Foster,
Youngjae You Tags: SPECIAL ISSUE RESEARCH ARTICLE Source Type: research
Aloe vera ‐derived graphene‐coupled phenosafranin photocatalyst for generation and regeneration of ammonia and NADH by mimicking natural photosynthetic route
In this study, the utilization of solar light irradiation enables the generation and regeneration of ammonia and NADH using an aloe vera-derived graphene-coupled phenosafranin (ADGCP) photocatalyst. The results show the remarkable efficiency and environmental friendly nature of the ADGCP system, offering the promising way to solve worldwide issues related to sustainable chemical synthesis and energy production. AbstractAloe vera-derived graphene (ADG) coupled system photocatalyst, mimicking natural photosynthesis, is one of the most promising ways for converting solar energy into ammonia (NH3) and nicotinamide adenine dinu...
Source: Photochemistry and Photobiology - July 18, 2023 Category: Science Authors: Ravindra K. Shukla,
Rajesh K. Yadav,
V. L. Gole,
Chae Yeong Na,
Gyoung Hwa Jeong,
Satyam Singh,
Jin ‐Ook Baeg,
Myong Yong Choi,
Navneet Kumar Gupta,
Tae Wu Kim Tags: RESEARCH ARTICLE Source Type: research
Distribution of photoactive β‐carboline alkaloids across Passiflora caerulea floral organs
This study reports valuable information regarding the presence and concentration of a series of photoactive β-carboline (βCs) alkaloids (norharmane, harmane, harmine, harmol, harmaline, and harmalol) and their distribution across the floral age and organs ofPassiflora caerulea. UHPLC –MS/MS data reported herein reveal that the βCs' content ranged from 1 to 110 μg kg−1, depending on the floral organ and age. In certain physiologically relevant organs, such as anthers, βCs' content was one order of magnitude higher than in other organs, suggesting a special role for βCs in this specific organ. βCs' content als...
Source: Photochemistry and Photobiology - July 14, 2023 Category: Science Authors: Brian Ospina ‐Calvo,
Eduardo De Gerónimo,
Fernando D. Villarruel,
Virgina C. Aparicio,
Lorena Ashworth,
Rosa Erra‐Balsells,
Franco M. Cabrerizo Tags: RESEARCH ARTICLE Source Type: research
Mechanistic photophysics of tellurium ‐substituted cytosine: Electronic structure calculations and nonadiabatic dynamics simulations
Excited-state decay mechanism of tellurium-substituted cytosine by QTMF-FSSH nonadiabatic dynamics simulations including internal conversion and intersystem crossing simultaneously. AbstractPreviously, the MS-CASPT2 method was performed to study the static and qualitative photophysics of tellurium-substituted cytosine (TeC). To get quantitative information, we used our recently developed QTMF-FSSH dynamics method to simulate the excited-state decay of TeC. The CASSCF method was adopted to reduce the calculation costs, which was confirmed to provide reliable structures and energies as those of MS-CASPT2. A detailed structur...
Source: Photochemistry and Photobiology - July 13, 2023 Category: Science Authors: Xue ‐Ping Chang,
Jie‐Lei Wang,
Ling‐Ya Peng,
Xu‐Jiang Cen,
Bo‐Wen Yin,
Bin‐Bin Xie Tags: SPECIAL ISSUE RESEARCH ARTICLE Source Type: research
Issue Information
(Source: Photochemistry and Photobiology)
Source: Photochemistry and Photobiology - July 11, 2023 Category: Science Tags: Issue Information Source Type: research
Critical PDT Theory V: What it Takes
First evidence for an apoptotic response to photodamage provided by Oleinick's group in 1991. (Source: Photochemistry and Photobiology)
Source: Photochemistry and Photobiology - July 11, 2023 Category: Science Authors: David Kessel Tags: Correspondence Source Type: research
Dual effect of light and ultrasound for efficient singlet oxygen generation with novel diaxial silicon phthalocyanine sensitizer
When sono-photochemical and photochemical methods are compared, it is seen that sono-photochemical studies are much more advantageous in terms of singlet oxygen yield. AbstractTo treat a life-threatening disease like cancer, photodynamic therapy (PDT) and sonodynamic therapy (SDT) methods were combined into sono-photodynamic therapy (SPDT) as an effective therapeutic solution. Each day, the usage of phthalocyanine sensitizers increases in the therapeutic applications as they have the ability to produce more reactive oxygen species. In this context, a new diaxially silicon phthalocyanine sensitizer, containing triazole and ...
Source: Photochemistry and Photobiology - July 11, 2023 Category: Science Authors: G ülşah Gümrükçü Köse,
Ali Erdoğmuş Tags: RESEARCH ARTICLE Source Type: research
The photophysics of protonated cytidine and hemiprotonated cytidine base pair: A computational study
Quantum mechanical calculations show that an easily accessible non-radiative deactivation route to the ground electronic state exists for photoexcited cytidine, protonated at acidic pH, also when hydrogen bonded to a “neutral” cytidine, forming the building blocks of DNA adopting an I-motif structure. AbstractWe here study the effect that a lowering of the pH has on the excited state processes of cytidine and a cytidine/cytidine pair in solution, by integrating time-dependent density functional theory and CASSCF/CASPT2 calculations, and including solvent by a mixed discrete/continuum model. Our calculations reproduce t...
Source: Photochemistry and Photobiology - July 7, 2023 Category: Science Authors: Lara Martinez ‐Fernandez,
Roberto Improta Tags: SPECIAL ISSUE RESEARCH ARTICLE Source Type: research
The copious photochemistry of 2,6 ‐diaminopurine: Luminescence, triplet population, and ground state recovery
2,6-Diaminopurine tautomers (9H- and 7H-) gas phase photoevents. For both tautomers, there is a competition between fluorescence and ultrafast internal conversion processes. In addition, for the 7H-tautomer, there is also the possibility of triplet state formation for 7HDAP from low and high excited singlet states. Abstract9H- and 7H-2,6-Diaminopurine (26DAP) photoinduced events in vacuum were studied at the MS-CASPT2/cc-pVDZ level of theory. The S11( ππ* La) state is initially populated evolving barrierless towards its minimum energy structure, from where two photochemical events can take place in both tautomers. The fi...
Source: Photochemistry and Photobiology - July 5, 2023 Category: Science Authors: Leonardo M. F. Oliveira,
Danillo Valverde,
Gustavo J. Costa,
Antonio Carlos Borin Tags: SPECIAL ISSUE RESEARCH ARTICLE Source Type: research
Antitumoral photoinduced effects of crude extract, fractions, and naphthoquinones from Sinningia magnifica (Otto & A. Dietr.) Wiehler (Gesneriaceae) in a bioguided study
The bioguided study ofSinningia magnifica (Otto& A. Dietr.) Wiehler crude extract and fractions reveals naphthoquinones by UHPLC –MS/MS identification as Dunniol and 7-hydroxy-6-methoxy-α-dunnione. In the photodegradation assay, singlet oxygen production was enhanced, showing the potential of this plant for use of its substances as natural photosensitizers against melanoma (SK-MEL-103) and prostate cancer (PC-3) cell lines , as demonstrated by our study for the first time. AbstractPhotodynamic therapy (PDT) has been used for various purposes, including as an antitumor resource in a noninvasive therapy with minimal side ...
Source: Photochemistry and Photobiology - July 4, 2023 Category: Science Authors: A. F. Serain,
A. J. Buitrago ‐Mejia,
G. C. A. Souza,
W. R. Corrêa,
M. E. A. Stefanello,
M. J. Salvador Tags: RESEARCH ARTICLE Source Type: research
Can l ‐ascorbic acid and trans‐resveratrol protect HaCaT cells from fine particulate matter toxicity?
Co-exposure of human skin to PM2.5 and solar radiation can lead to undesirable skin conditions.l-ascorbic acid and resveratrol were tested as potential topical agents against pollution-related skin impairment taking seasonal variations of particles into account. EPR assays and singlet oxygen phosphorescence detection were used to determine scavenging activities of antioxidants. The protective potential of antioxidants on PM2.5-exposed cells was demonstrated using MTT, JC-10 and iodometric assays and visualized using immunofluorescent staining. The stimulating effect on wound-healing was shown using live-cell imaging. Both ...
Source: Photochemistry and Photobiology - June 27, 2023 Category: Science Authors: Krystian Mokrzy ński,
Olga Krzysztyńska‐Kuleta,
Mateusz Wojtala,
Dawid Wnuk,
Michał Sarna,
Tadeusz Sarna Tags: RESEARCH ARTICLE Source Type: research
Processes triggered in guanine quadruplexes by direct absorption of UV radiation: From fundamental studies toward optoelectronic biosensors
The primary processes induced in guanine quadruplexes (GQs) by direct UV absorption have been studied by time-resolved spectroscopy, associated with computational methods. The present review highlights the outcomes of these fundamental studies that could contribute to the development of optoelectronic biosensors using GQ intrinsic signals (label-free, dye-free), explored recently by a few groups. It explains how certain structural factors, intervening during the excited state r elaxation, affect both fluorescence emission and low-energy photoionization generating charge carriers. Accordingly, these features can be tuned ...
Source: Photochemistry and Photobiology - June 27, 2023 Category: Science Authors: Dimitra Markovitsi Tags: INVITED REVIEW Source Type: research
