New insights into the effects of UV light on individual Nosema bombycis spores, as determined using single ‐cell optical approaches
This study provides new insights into the molecular mechanisms underlying conventional disinfection measures on microsporidian spores. (Source: Photochemistry and Photobiology)
Source: Photochemistry and Photobiology - September 12, 2023 Category: Science Authors: Zhenbin Miao,
Yanlian Liao,
Xuhua Huang,
Xiaochun Wang,
Wei Liao,
Guiwen Wang Tags: RESEARCH ARTICLE Source Type: research
Making the sunshine vitamin – How much sun exposure is needed to maintain 25‐hydroxy vitamin D concentration?
We used a microsimulation model to estimate changes in monthly 25(OH)D concentration using data on standard erythemal dose, solar zenith angle, and climatological ozone. We estimated the number of standard vitamin D doses per 10-min interval and used a dose-response equation to determine the minimum time in the sun to maintain existing 25(OH)D concentration according to month and time of day. The minutes of sun exposure for Brisbane, Sydney, Melbourne, and Christchurch are displayed. AbstractOur objective was to calculate the time in the sun necessary to maintain existing 25-hydroxyvitamin D (25(OH)D) concentration at loca...
Source: Photochemistry and Photobiology - September 11, 2023 Category: Science Authors: Thomas M. Elliott,
Louisa G. Gordon,
Ann Webb,
Richard Kift,
Anna Foeglein,
Rachel E. Neale Tags: RESEARCH ARTICLE Source Type: research
Ultrafast excited state dynamics of isocytosine unveiled by femtosecond broadband time ‐resolved spectroscopy combined with density functional theoretical study
In this report, we present an investigation of the excited state dynamics of isocytosine in both protic and aprotic solvents, which was conducted by a combination of methods including steady-state spectroscopy, femtosecond broadband time-resolved fluorescence, and transient absorption. These methods were coupled with density functional and time-dependent density functional theory calculations. The results of our study provide the first direct evidence for a highly efficient nonradiative mechanism achieved through internal conversion from the ππ* state of the isocytosine keto-N(3)H form occurring within subpicoseconds and...
Source: Photochemistry and Photobiology - September 9, 2023 Category: Science Authors: Chensheng Ma,
Qingwu Xiong,
Jingdong Lin,
Allen Ka ‐Wa Wong,
Mingliang Wang,
Wai‐Ming Kwok Tags: SPECIAL ISSUE RESEARCH ARTICLE Source Type: research
Evaluation of human skin response to solar ‐simulated radiation in an ex vivo model: Effects and photoprotection of L‐Carnosine
In conclusion, we report that our ex vivo skin mo del is a valuable system to assess the consequences of solar light exposure and the capacity of topically applied L-Carnosine to counteract them. (Source: Photochemistry and Photobiology)
Source: Photochemistry and Photobiology - September 8, 2023 Category: Science Authors: Cristina Girardi,
Francesca Benato,
Marco Massironi,
Vincenzo Vindigni,
Dominik Stuhlmann,
Michele Massironi Tags: RESEARCH ARTICLE Source Type: research
Time ‐resolved fluorescence anisotropy with Atto 488‐labeled phytochrome Agp1 from Agrobacterium fabrum
The cartoon shows a structural model of the phytochrome Agp1 fromAgrobacterium fabrum. The colored spheres indicate positions where Cys residues were introduced for labeling with the fluorescent Atto-488. Flexibility of protein regions was determined at the different positions in the Pr and Pfr forms of the phytochrome by time-resolved fluorescence anisotropy. Green, PAS domain; blue, GAF domain; yellow, PHY domain; orange, Dhp domain of histidine kinase; magenta, ATPase domain of histidine kinase. Flexibility parameters differed significantly between the different regions and between full-length and protein without histid...
Source: Photochemistry and Photobiology - September 7, 2023 Category: Science Authors: Afaf Elkurdi,
Gernot Guigas,
Latifa Hourani ‐Alsharafat,
Patrick Scheerer,
Gerd Ulrich Nienhaus,
Norbert Krauß,
Tilman Lamparter Tags: RESEARCH ARTICLE Source Type: research
Rutin intake mitigates the injury of blue light irradiation by altering aging rates of mortality in Drosophila model
Under short wavelength blue light exposure, the activity and average lifespan ofDrosophila fed with rutin are both enhanced. AbstractBlue light is known as one of the harmful light pollution that has complex effects on organisms. The massive use of LED lights in cities has greatly increased the frequency of human exposure to blue light, and therefore the hazards of blue light are receiving widespread attention. In our study,Drosophila was used as the model organism to explore the ability of the flavonoid rutin to resist blue light damage under the intensity of 3000 Lux. Siler model analysis was performed. Our results showe...
Source: Photochemistry and Photobiology - September 5, 2023 Category: Science Authors: Hao Tang,
Xiangyu Li,
Xingyou Liu,
Yifan Xu,
Jie Shen Tags: RAPID COMMUNICATION Source Type: research
Role of curvature in acridone for 1O2 oxidation of a natural product homoallylic alcohol: A novel iso ‐hydroperoxide intermediate
Strategies for improving1O2 oxidation paths to dihydrobenzofurans are needed. A density functional theory study provides evidence that acridone curvature and phenol O –H···π bonding facilitate1O2 oxidation of a prenyl side group to reach a dihydrobenzofuran. Mechanistic insight is provided for aniso-hydroperoxide intermediate preceding the dihydrobenzofuran. Notably, our evidence for theiso-hydroperoxide intermediate is reminiscent of otheriso species, such as oxywater andiso-iodoform in fields outside biosynthetic and1O2 chemistry. AbstractA density functional theoretical (DFT) study is presented, implicating a1O2 o...
Source: Photochemistry and Photobiology - August 22, 2023 Category: Science Authors: Lloyd Lapoot,
Shakeela Jabeen,
Andr és M. Durantini,
Alexander Greer Tags: RESEARCH ARTICLE Source Type: research
Is sunscreen alone effective at preventing sunburn on a high ‐solar beach vacation: A modeling study?
Beach holidays in areas of strong sunlight are popular and sunscreen is often the primary means of photoprotection favored by many people. In this article, we examine how effective sunscreen is in preventing sunburn on a sun-seeking holiday that combines prolonged exposure with high UV levels. We used a computational model to determine how the quantity, frequency, substantivity, and labeled SPF of applied sunscreens impact on the predicted erythemal response in unacclimatized skin over the course of a 7-day holiday and our results indicated that sunscreen on its own may be insufficient to prevent a sunburn-free vacation. A...
Source: Photochemistry and Photobiology - August 21, 2023 Category: Science Authors: Brian L. Diffey,
Alois W. Schmalwieser Tags: RESEARCH ARTICLE Source Type: research
Coupling of NAD(P)H:FMN ‐oxidoreductase and luciferase from luminous bacteria in a viscous medium: Finding the weakest link in the chain
This study examined the effects of glycerol and sucrose on the coupled reactions of NAD(P)H:FMN-oxidoreductase and luciferase of luminous bacteria in vitro, providing valuable information regarding microenvironment of the enzyme and its function. AbstractThe study aims at revealing the mechanisms of the viscous medium effects on the kinetic features of NAD(P)H:FMN-oxidoreductase from luminous bacteria (Red), which are exhibited in a single enzyme assay and in coupling with bacterial luciferase (BLuc). Different concentrations of glycerol and sucrose were used to vary the medium viscosity. The activity of Red, alone and in ...
Source: Photochemistry and Photobiology - August 16, 2023 Category: Science Authors: Oleg S. Sutormin,
Elena V. Nemtseva,
Dmitry V. Gulnov,
Lev A. Sukovatyi,
Yekaterina S. Tyrtyshnaya,
Albert E. Lisitsa,
Valentina A. Kratasyuk Tags: RESEARCH ARTICLE Source Type: research
Warming exacerbates the impacts of ultraviolet radiation in temperate diatoms but alleviates the effect on polar species
Under global change scenarios, increasing sea surface temperatures and other factors have led to changes in oceanic environments. Phytoplankton, including marine diatoms, are exposed to multiple interacting drivers. The responses of polar and temperate diatoms to UV radiation differ. We exposedEntomoneis sp.,Thalassiosira weissflogii, andSkeletonema costatum to warming for 10 days and performed incubations under different radiation treatments. UV-induced inhibition was observed, with highest inhibition in polar diatom. However, warming alleviated UV-induced inhibition in polar diatom, while increasing the UV inhibition ...
Source: Photochemistry and Photobiology - August 2, 2023 Category: Science Authors: Lixin Cao,
Dongquan Bi,
Wei Fan,
Juntian Xu,
John Beardall,
Kunshan Gao,
Yaping Wu Tags: RESEARCH NOTE Source Type: research
Test method for evaluating the photocytotoxic potential of fluorescence imaging products
This study provides evidence and insights into practices useful for testing the photochemical safety of fluorescence imaging products. (Source: Photochemistry and Photobiology)
Source: Photochemistry and Photobiology - July 27, 2023 Category: Science Authors: Shruti Vig,
Brandon Gaitan,
Lucas Frankle,
Yu Chen,
Rosalie Elespuru,
T. Joshua Pfefer,
Huang ‐Chiao Huang Tags: SPECIAL ISSUE RESEARCH ARTICLE Source Type: research
Multi ‐omics approach to understand the impact of sun exposure on an in vitro skin ecosystem and evaluate a new broad‐spectrum sunscreen
This study identified highly accurate metabolomic fingerprints and metagenomic modifications of sun-exposed skin. Application of SPF50+ sunscreen protected the skin ecosystem model from the deleterious effects of SSR and preserved skin –microbiota interactions. AbstractA reconstructed human epidermal model (RHE) colonized with human microbiota and sebum was developed to reproduce the complexity of the skin ecosystem in vitro. The RHE model was exposed to simulated solar radiation (SSR) with or without SPF50+ sunscreen (with UVB, UVA, long-UVA, and visible light protection). Structural identification of discriminant metab...
Source: Photochemistry and Photobiology - July 25, 2023 Category: Science Authors: Carine Jacques,
Daniel Bacqueville,
Emilien L. Jamin,
Martine Maitre,
C écile Delsol,
Aimée Simcic‐Mori,
Pascale Bianchi,
Anais Noustens,
Isabelle Jouanin,
Laurent Debrauwer,
Sandrine Bessou‐Touya,
Eggert Stockfleth,
Hélène Duplan Tags: RESEARCH ARTICLE Source Type: research
Critical PDT theory: Viability, cytotoxicity, proliferation and clonogenicity —Is there a difference?
Comparison of MTT versus clonogenic effects of photodynamic therapy in MCF-7c3 breast cancer cells in culture. Left: MTT data; right clonogenic data. A collection of photosensitizers was used to produce these results. (Source: Photochemistry and Photobiology)
Source: Photochemistry and Photobiology - July 24, 2023 Category: Science Authors: David Kessel Tags: CORRESPONDENCE Source Type: research
Mutagenicity assessment of high ‐power 1.6‐THz pulse laser radiation
The present study aimed to assess the mutagenicity of 1.6 THz laser irradiation in the Ames test. The single bacterial colony of tester strains (TA98, TA100, TA1535, TA1537, and WP2uvrA) was irradiated with a 1.6 THz laser using a tabletop THz pulse laser system. The results showed that the THz laser irradiation did not increase the number of revertant colonies compared to that in the negative control group, whereas the positive control group treated with chemicals known as mutagens increased the number of revertant colonies. Thus, it is suggested that 1.6 THz laser irradiation is unlikely to be mutagenic. AbstractThe effe...
Source: Photochemistry and Photobiology - July 22, 2023 Category: Science Authors: Midori Shirato,
Yuma Takida,
Taro Kanno,
Hiroshi Matsuura,
Yoshimi Niwano,
Hiroaki Minamide,
Keisuke Nakamura Tags: RESEARCH ARTICLE Source Type: research
Rose bengal –encapsulated chitosan nanoparticles for the photodynamic treatment of Trichophyton species
Encapsulated rose bengal nanoparticles in combination with photodynamic therapy are highly effective for eradicating clinically relevantTrichophyton spores that cause onychomycosis. AbstractRose bengal (RB) solutions coupled with a green laser have proven to be efficient in clearing resilient nail infections caused byTrichophyton rubrum in a human pilot study and in extensive in vitro experiments. Nonetheless, the RB solution can become diluted or dispersed over the tissue and prevented from penetrating the nail plate to reach the subungual area where fungal infection proliferates. Nanoparticles carrying RB can mitigate th...
Source: Photochemistry and Photobiology - July 21, 2023 Category: Science Authors: Alina Bekmukhametova,
Anu Antony,
Catriona Halliday,
Sharon Chen,
Chun ‐Hoong Ho,
Mir Muhammad Nasir Uddin,
Leonardo Longo,
Christian Pedrinazzi,
Laurel George,
Richard Wuhrer,
Simon Myers,
Damia Mawad,
Jessica Houang,
Antonio Lauto Tags: RESEARCH ARTICLE Source Type: research
