Memory-like NK Cells Are a Critical Component of Vaccine-Induced Immunity to Trypanosoma cruzi Infection
J Immunol. 2024 Feb 15;212(4):617-631. doi: 10.4049/jimmunol.2300509.ABSTRACTChagas disease by Trypanosoma cruzi infection is a major public health issue. The available therapeutic agents have limited efficacy and significant side effects. A reliable vaccine would reduce the threat of T. cruzi infections and prevent Chagas disease. Understanding the immune response to this infection would improve vaccine design. We previously demonstrated that adoptively transferred NK cells from mice immunized with highly attenuated T. cruzi, GFP-DDDHA strain, provided potent protection in naive recipients against secondary lethal challen...
Source: Journal of Immunology - January 10, 2024 Category: Allergy & Immunology Authors: Aline L Horta Jason Gigley Marie Boutet Gregoire Lavau Louis M Weiss Huan Huang Source Type: research
Vaccine Molecule Design Based on Phage Display and Computational Modeling against Rhabdovirus
In this study, fish-derived Abs are employed for a Micropterus salmoides rhabdovirus (MSRV) vaccine design by phage display and bioinformatics analysis. We constructed an anti-MSRV phage Ab library to screen Abs for glycoprotein segment 2 (G2) (G129-266). Four M13-phage-displayed Abs (Ab-5, Ab-7, Ab-8 and Ab-30) exhibited strong specificity to target Ag, and Ab-7 had the highest affinity with MSRV. Ab-7 (300 μg/ml) significantly increased grass carp ovary cell viability to 83.40% and significantly decreased the titer of MSRV. Molecular docking results showed that the key region of Ag-Ab interaction was located in 10ESQEFT...
Source: Journal of Immunology - January 10, 2024 Category: Allergy & Immunology Authors: Yu-Ying Zheng Liang Zhao Xue-Feng Wei Tian-Zi Sun Fei-Fan Xu Gao-Xue Wang Bin Zhu Source Type: research
Glycolysis Induced by METTL14 Is Essential for Macrophage Phagocytosis and Phenotype in Cervical Cancer
In this study, m6A methylated RNA immunoprecipitation sequencing analysis revealed the m6A profiles in cervical cancer. Then, we discovered that the high expression of METTL14 (methyltransferase 14, N6-adenosine-methyltransferase subunit) in cervical cancer tissues can promote the proportion of programmed cell death protein 1 (PD-1)-positive tumor-associated macrophages, which have an obstacle to devour tumor cells. Functionally, changes of METTL14 in cervical cancer inhibit the recognition and phagocytosis of macrophages to tumor cells. Mechanistically, the abnormality of METTL14 could target the glycolysis of tumors in v...
Source: Journal of Immunology - January 10, 2024 Category: Allergy & Immunology Authors: Bingyu Wang Zhonghao Mao Jinwen Ye Xinlin Jiao Teng Zhang Qi Wang Sai Han Youzhong Zhang Chunling Wang Taotao Dong Baoxia Cui Source Type: research
Cutting Edge: Characterization of Low Copy Number Human Angiotensin-Converting Enzyme 2-Transgenic Mice as an Improved Model of SARS-CoV-2 Infection
J Immunol. 2024 Feb 15;212(4):523-528. doi: 10.4049/jimmunol.2300591.ABSTRACTA popular mouse model of COVID-19, the K18-hACE2 mouse, expresses the SARS-coronavirus entry receptor, human angiotensin-converting enzyme 2 (hACE2) driven by the keratin-18 promoter. SARS-CoV-2-infected K18-hACE2 mice exhibit neuropathology not representative of human infection. They contain eight transgene (Tg) copies, leading to excess hACE2 expression and rampant viral replication. We generated two new lines of K18-hACE2 mice encoding one and two copies of hACE2 (1-hACE2-Tg and 2-hACE2-Tg, respectively). Relative to the original strain (called...
Source: Journal of Immunology - January 10, 2024 Category: Allergy & Immunology Authors: Christine M Bradshaw Teodora Georgieva Trevor N Tankersley Tama Taylor-Doyle Larry Johnson Jennifer L Uhrlaub David Besselsen Janko Ž Nikolich Source Type: research
Reduced Expression of miR-146a Potentiates Intestinal Inflammation following Alcohol and Burn Injury
In this study, we sought to profile changes in intestinal epithelial cell miRNA expression after alcohol and burn injury and elucidate their impact on inflammation and barrier integrity. Using a mouse model of acute ethanol intoxication and burn injury, we found that small intestinal epithelial cell expression of miR-146a is significantly decreased 1 d following injury. Using in vitro studies, we show that reduced miR-146a promotes intestinal epithelial cell inflammation by promoting p38 MAPK signaling via increased levels of its target TRAF6 (TNFR-associated factor 6). Furthermore, we demonstrate that in vivo miR-146a ove...
Source: Journal of Immunology - January 8, 2024 Category: Allergy & Immunology Authors: Caroline J Herrnreiter Marisa E Luck Abigail R Cannon Xiaoling Li Mashkoor A Choudhry Source Type: research
Reduced Expression of miR-146a Potentiates Intestinal Inflammation following Alcohol and Burn Injury
In this study, we sought to profile changes in intestinal epithelial cell miRNA expression after alcohol and burn injury and elucidate their impact on inflammation and barrier integrity. Using a mouse model of acute ethanol intoxication and burn injury, we found that small intestinal epithelial cell expression of miR-146a is significantly decreased 1 d following injury. Using in vitro studies, we show that reduced miR-146a promotes intestinal epithelial cell inflammation by promoting p38 MAPK signaling via increased levels of its target TRAF6 (TNFR-associated factor 6). Furthermore, we demonstrate that in vivo miR-146a ove...
Source: Journal of Immunology - January 8, 2024 Category: Allergy & Immunology Authors: Caroline J Herrnreiter Marisa E Luck Abigail R Cannon Xiaoling Li Mashkoor A Choudhry Source Type: research
Antiviral and Anti-Inflammatory Therapeutic Effect of RAGE-Ig Protein against Multiple SARS-CoV-2 Variants of Concern Demonstrated in K18-hACE2 Mouse and Syrian Golden Hamster Models
J Immunol. 2024 Jan 5:ji2300392. doi: 10.4049/jimmunol.2300392. Online ahead of print.ABSTRACTSARS-CoV-2 variants of concern (VOCs) continue to evolve and reemerge with chronic inflammatory long COVID sequelae, necessitating the development of anti-inflammatory therapeutic molecules. Therapeutic effects of the receptor for advanced glycation end products (RAGE) were reported in many inflammatory diseases. However, a therapeutic effect of RAGE in COVID-19 has not been reported. In the present study, we investigated whether and how the RAGE-Ig fusion protein would have an antiviral and anti-inflammatory therapeutic effect in...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Nisha Rajeswari Dhanushkodi Swayam Prakash Afshana Quadiri Latifa Zayou Ruchi Srivastava Amin Mohammed Shaik Berfin Suzer Izabela Coimbra Ibraim Gary Landucci Delia F Tifrea Mahmoud Singer Leila Jamal Robert A Edwards Hawa Vahed Lawrence Brown Lbachir Ben Source Type: research
CL-K1 Promotes Complement Activation and Regulates Opsonophagocytosis of Macrophages with CD93 Interaction in a Primitive Vertebrate
In this study, the Oreochromis niloticus CL-K1 (OnCL-K1) protein was purified and identified, which was capable of binding to two important pathogens of tilapia, Streptococcus agalactiae and Aeromonas hydrophila. Interestingly, OnCL-K1 exhibited direct bactericidal activity by binding to lipoteichoic acid or LPS on cell walls, disrupting the permeability and integrity of the bacterial membrane in vitro. Upon bacterial challenge, OnCL-K1 significantly inhibited the proliferation of pathogenic bacteria, reduced the inflammatory response, and improved the survival of tilapia. Further research revealed that OnCL-K1 could assoc...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Liangliang Mu Xiaoxue Yin Li Qiu Jiadong Li Jinfen Mo Hao Bai Qingliang Zeng Jianmin Ye Source Type: research
Recruitment and Maintenance of CX3CR1+CD4+ T Cells during Helminth Infection
In this study, we used a fate-mapping mouse model to characterize CX3CR1+CD4+ T cells during both acute Nippostrongylus brasiliensis and chronic Schistosoma mansoni murine models of helminth infections, revealing CX3CR1+CD4+ T cells to be an activated tissue-homing subset with varying capacity for cytokine production. Tracking these cells over time revealed that maintenance of CX3CR1 itself along with a TH2 phenotype conferred a survival advantage in the inflamed tissue. Single-cell RNA sequencing analysis of fate-mapped CX3CR1+CD4+ T cells from both the peripheral tissue and the spleen revealed a considerable level of div...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Denis G Loredan Joseph C Devlin Kamal M Khanna P'ng Loke Source Type: research
Antiviral and Anti-Inflammatory Therapeutic Effect of RAGE-Ig Protein against Multiple SARS-CoV-2 Variants of Concern Demonstrated in K18-hACE2 Mouse and Syrian Golden Hamster Models
J Immunol. 2024 Jan 5:ji2300392. doi: 10.4049/jimmunol.2300392. Online ahead of print.ABSTRACTSARS-CoV-2 variants of concern (VOCs) continue to evolve and reemerge with chronic inflammatory long COVID sequelae, necessitating the development of anti-inflammatory therapeutic molecules. Therapeutic effects of the receptor for advanced glycation end products (RAGE) were reported in many inflammatory diseases. However, a therapeutic effect of RAGE in COVID-19 has not been reported. In the present study, we investigated whether and how the RAGE-Ig fusion protein would have an antiviral and anti-inflammatory therapeutic effect in...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Nisha Rajeswari Dhanushkodi Swayam Prakash Afshana Quadiri Latifa Zayou Ruchi Srivastava Amin Mohammed Shaik Berfin Suzer Izabela Coimbra Ibraim Gary Landucci Delia F Tifrea Mahmoud Singer Leila Jamal Robert A Edwards Hawa Vahed Lawrence Brown Lbachir Ben Source Type: research
CL-K1 Promotes Complement Activation and Regulates Opsonophagocytosis of Macrophages with CD93 Interaction in a Primitive Vertebrate
In this study, the Oreochromis niloticus CL-K1 (OnCL-K1) protein was purified and identified, which was capable of binding to two important pathogens of tilapia, Streptococcus agalactiae and Aeromonas hydrophila. Interestingly, OnCL-K1 exhibited direct bactericidal activity by binding to lipoteichoic acid or LPS on cell walls, disrupting the permeability and integrity of the bacterial membrane in vitro. Upon bacterial challenge, OnCL-K1 significantly inhibited the proliferation of pathogenic bacteria, reduced the inflammatory response, and improved the survival of tilapia. Further research revealed that OnCL-K1 could assoc...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Liangliang Mu Xiaoxue Yin Li Qiu Jiadong Li Jinfen Mo Hao Bai Qingliang Zeng Jianmin Ye Source Type: research
Recruitment and Maintenance of CX3CR1+CD4+ T Cells during Helminth Infection
In this study, we used a fate-mapping mouse model to characterize CX3CR1+CD4+ T cells during both acute Nippostrongylus brasiliensis and chronic Schistosoma mansoni murine models of helminth infections, revealing CX3CR1+CD4+ T cells to be an activated tissue-homing subset with varying capacity for cytokine production. Tracking these cells over time revealed that maintenance of CX3CR1 itself along with a TH2 phenotype conferred a survival advantage in the inflamed tissue. Single-cell RNA sequencing analysis of fate-mapped CX3CR1+CD4+ T cells from both the peripheral tissue and the spleen revealed a considerable level of div...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Denis G Loredan Joseph C Devlin Kamal M Khanna P'ng Loke Source Type: research
Antiviral and Anti-Inflammatory Therapeutic Effect of RAGE-Ig Protein against Multiple SARS-CoV-2 Variants of Concern Demonstrated in K18-hACE2 Mouse and Syrian Golden Hamster Models
J Immunol. 2024 Jan 5:ji2300392. doi: 10.4049/jimmunol.2300392. Online ahead of print.ABSTRACTSARS-CoV-2 variants of concern (VOCs) continue to evolve and reemerge with chronic inflammatory long COVID sequelae, necessitating the development of anti-inflammatory therapeutic molecules. Therapeutic effects of the receptor for advanced glycation end products (RAGE) were reported in many inflammatory diseases. However, a therapeutic effect of RAGE in COVID-19 has not been reported. In the present study, we investigated whether and how the RAGE-Ig fusion protein would have an antiviral and anti-inflammatory therapeutic effect in...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Nisha Rajeswari Dhanushkodi Swayam Prakash Afshana Quadiri Latifa Zayou Ruchi Srivastava Amin Mohammed Shaik Berfin Suzer Izabela Coimbra Ibraim Gary Landucci Delia F Tifrea Mahmoud Singer Leila Jamal Robert A Edwards Hawa Vahed Lawrence Brown Lbachir Ben Source Type: research
CL-K1 Promotes Complement Activation and Regulates Opsonophagocytosis of Macrophages with CD93 Interaction in a Primitive Vertebrate
In this study, the Oreochromis niloticus CL-K1 (OnCL-K1) protein was purified and identified, which was capable of binding to two important pathogens of tilapia, Streptococcus agalactiae and Aeromonas hydrophila. Interestingly, OnCL-K1 exhibited direct bactericidal activity by binding to lipoteichoic acid or LPS on cell walls, disrupting the permeability and integrity of the bacterial membrane in vitro. Upon bacterial challenge, OnCL-K1 significantly inhibited the proliferation of pathogenic bacteria, reduced the inflammatory response, and improved the survival of tilapia. Further research revealed that OnCL-K1 could assoc...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Liangliang Mu Xiaoxue Yin Li Qiu Jiadong Li Jinfen Mo Hao Bai Qingliang Zeng Jianmin Ye Source Type: research
Recruitment and Maintenance of CX3CR1+CD4+ T Cells during Helminth Infection
In this study, we used a fate-mapping mouse model to characterize CX3CR1+CD4+ T cells during both acute Nippostrongylus brasiliensis and chronic Schistosoma mansoni murine models of helminth infections, revealing CX3CR1+CD4+ T cells to be an activated tissue-homing subset with varying capacity for cytokine production. Tracking these cells over time revealed that maintenance of CX3CR1 itself along with a TH2 phenotype conferred a survival advantage in the inflamed tissue. Single-cell RNA sequencing analysis of fate-mapped CX3CR1+CD4+ T cells from both the peripheral tissue and the spleen revealed a considerable level of div...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Denis G Loredan Joseph C Devlin Kamal M Khanna P'ng Loke Source Type: research
