Expression of Concern: Phosphatase holoenzyme PP1/GADD34 negatively regulates TLR response by inhibiting TAK1 serine 412 phosphorylation
J Immunol. 2024 Mar 1;212(5):928. doi: 10.4049/jimmunol.2400013.NO ABSTRACTPMID:38377478 | DOI:10.4049/jimmunol.2400013 (Source: Journal of Immunology)
Source: Journal of Immunology - February 20, 2024 Category: Allergy & Immunology Authors: Meidi Gu Chuan Ouyang Wenlong Lin Ting Zhang Xuetao Cao Zongping Xia Xiaojian Wang Source Type: research
Molecular Interactions Required for Activation of Complement Component C2 Include Exosites Located on the Serine Protease Domain of C1s and Mannose-Binding Lectin Associated Protease-2
In this study, we have shown that the ABE on the SP of both C1s and MASP-2 is crucial for efficient cleavage of C2, with mutant forms of the proteases greatly impaired in their rate of cleavage of C2. We have additionally shown that the site of binding for the ABE of the proteases is very likely to be located on the von Willebrand factor domain of C2, with the precise area differing between the enzymes: whereas C1s requires two anionic clusters on the von Willebrand factor domain to enact efficient cleavage of C2, MASP-2 apparently only requires one. These data provide (to our knowledge) new information about the molecular...
Source: Journal of Immunology - February 19, 2024 Category: Allergy & Immunology Authors: Lilian Hor Jing Pan Robert N Pike Lakshmi C Wijeyewickrema Source Type: research
Role for Caspase-8 in the Release of IL-1 β and Active Caspase-1 from Viable Human Monocytes during Toxoplasma gondii Infection
This study expands on the molecular pathways that promote IL-1β in human immune cells and provides evidence of a role for caspase-8 in the mechanism of IL-1β release during infection.PMID:38372637 | DOI:10.4049/jimmunol.2200513 (Source: Journal of Immunology)
Source: Journal of Immunology - February 19, 2024 Category: Allergy & Immunology Authors: William J Pandori Stephanie Y Matsuno Ji-Hun Shin Samuel C Kim Tiffany H Kao Sharmila Mallya Sarah N Batarseh Melissa B Lodoen Source Type: research
Critical Role of CD55 in Controlling Wound Healing
In this study, we examined one physiological consequence of this signaling circuit. We found that disabling CD55 (also known as decay accelerating factor), which lifts restraint on autocrine C3ar1/C5ar1 signaling, concomitantly augments the growth of each cell type. The mechanism is heightened C3ar1/C5ar1 signaling resulting from the loss of CD55's restraint jointly potentiating growth factor production by each cell type. Examination of the effect of lifted CD55 restraint in four types of injury (burn, corneal denudation, ear lobe puncture, and reengraftment of autologous skin) showed that disabled CD55 function robustly a...
Source: Journal of Immunology - February 19, 2024 Category: Allergy & Immunology Authors: Lorna Kang Maryo Kohen Isaac McCarthy Emma Hammelef Hae Suk Kim R Bapputty Rose Gubitosi-Klug Faruk H Orge Timothy Kern M Edward Medof Source Type: research
Molecular Interactions Required for Activation of Complement Component C2 Include Exosites Located on the Serine Protease Domain of C1s and Mannose-Binding Lectin Associated Protease-2
In this study, we have shown that the ABE on the SP of both C1s and MASP-2 is crucial for efficient cleavage of C2, with mutant forms of the proteases greatly impaired in their rate of cleavage of C2. We have additionally shown that the site of binding for the ABE of the proteases is very likely to be located on the von Willebrand factor domain of C2, with the precise area differing between the enzymes: whereas C1s requires two anionic clusters on the von Willebrand factor domain to enact efficient cleavage of C2, MASP-2 apparently only requires one. These data provide (to our knowledge) new information about the molecular...
Source: Journal of Immunology - February 19, 2024 Category: Allergy & Immunology Authors: Lilian Hor Jing Pan Robert N Pike Lakshmi C Wijeyewickrema Source Type: research
Role for Caspase-8 in the Release of IL-1 β and Active Caspase-1 from Viable Human Monocytes during Toxoplasma gondii Infection
This study expands on the molecular pathways that promote IL-1β in human immune cells and provides evidence of a role for caspase-8 in the mechanism of IL-1β release during infection.PMID:38372637 | DOI:10.4049/jimmunol.2200513 (Source: Journal of Immunology)
Source: Journal of Immunology - February 19, 2024 Category: Allergy & Immunology Authors: William J Pandori Stephanie Y Matsuno Ji-Hun Shin Samuel C Kim Tiffany H Kao Sharmila Mallya Sarah N Batarseh Melissa B Lodoen Source Type: research
Critical Role of CD55 in Controlling Wound Healing
In this study, we examined one physiological consequence of this signaling circuit. We found that disabling CD55 (also known as decay accelerating factor), which lifts restraint on autocrine C3ar1/C5ar1 signaling, concomitantly augments the growth of each cell type. The mechanism is heightened C3ar1/C5ar1 signaling resulting from the loss of CD55's restraint jointly potentiating growth factor production by each cell type. Examination of the effect of lifted CD55 restraint in four types of injury (burn, corneal denudation, ear lobe puncture, and reengraftment of autologous skin) showed that disabled CD55 function robustly a...
Source: Journal of Immunology - February 19, 2024 Category: Allergy & Immunology Authors: Lorna Kang Maryo Kohen Isaac McCarthy Emma Hammelef Hae Suk Kim R Bapputty Rose Gubitosi-Klug Faruk H Orge Timothy Kern M Edward Medof Source Type: research
Fish Uses CTLA-4 Immune Checkpoint to Suppress mTORC1-Controlled T-Cell Glycolysis and Immunity
J Immunol. 2024 Feb 16:ji2300599. doi: 10.4049/jimmunol.2300599. Online ahead of print.ABSTRACTAs an immune checkpoint, cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) suppresses the activation, proliferation, and effector function of T cells, thus preventing an overexuberant response and maintaining immune homeostasis. However, whether and how this immune checkpoint functions in early vertebrates remains unknown. In the current study, using a Nile tilapia (Oreochromis niloticus) model, we investigated the suppression of T cell response by CTLA-4 in bony fish. Tilapia CTLA-4 is constitutively expressed in lymphoid tis...
Source: Journal of Immunology - February 16, 2024 Category: Allergy & Immunology Authors: Jiansong Zhang Xiumei Wei Qian Zhang Xinying Jiao Kang Li Ming Geng Yi Cao Ding Wang Jie Cheng Jialong Yang Source Type: research
Cutting Edge: The Tetraspanin CD53 Promotes CXCR4 Signaling and Bone Marrow Homing in B Cells
In this study, we identified the tetraspanin CD53 as a critical regulator of both normal and malignant B cell trafficking. CXCL12 is a key chemokine in B cell homing to the bone marrow and secondary lymphoid organs, and both normal and malignant B cells from Cd53-/- mice have reduced migration toward CXCL12 in vitro, as well as impaired marrow homing in vivo. Using proximity ligation studies, we identified the CXCL12 receptor, CXCR4, as a novel, to our knowledge, CD53 binding partner. This interaction promotes receptor function, because Cd53-/- B cells display reduced signaling and internalization of CXCR4 in response to C...
Source: Journal of Immunology - February 16, 2024 Category: Allergy & Immunology Authors: Mousumi Chakraborty Zev J Greenberg Qian Dong Nate Roundy Jeffrey J Bednarski Luana Chiquetto Paracatu Eric Duncavage Weikai Li Laura G Schuettpelz Source Type: research
Cytotoxic Programming of CD4+ T Cells Is Regulated by Opposing Actions of the Related Transcription Factors Eos and Aiolos
J Immunol. 2024 Feb 16:ji2300748. doi: 10.4049/jimmunol.2300748. Online ahead of print.ABSTRACTIn contrast to the "helper" activities of most CD4+ T effector subsets, CD4+ cytotoxic T lymphocytes (CD4-CTLs) perform functions normally associated with CD8+ T and NK cells. Specifically, CD4-CTLs secrete cytotoxic molecules and directly target and kill compromised cells in an MHC class II-restricted fashion. The functions of these cells have been described in diverse immunological contexts, including their ability to provide protection during antiviral and antitumor responses, as well as being implicated in autoimmunity. Despi...
Source: Journal of Immunology - February 16, 2024 Category: Allergy & Immunology Authors: Devin M Jones Jasmine A Tuazon Kaitlin A Read Melissa R Leonard Srijana Pokhrel Bharath K Sreekumar Robert T Warren Jacob S Yount Patrick L Collins Kenneth J Oestreich Source Type: research
Fish Uses CTLA-4 Immune Checkpoint to Suppress mTORC1-Controlled T-Cell Glycolysis and Immunity
J Immunol. 2024 Feb 16:ji2300599. doi: 10.4049/jimmunol.2300599. Online ahead of print.ABSTRACTAs an immune checkpoint, cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) suppresses the activation, proliferation, and effector function of T cells, thus preventing an overexuberant response and maintaining immune homeostasis. However, whether and how this immune checkpoint functions in early vertebrates remains unknown. In the current study, using a Nile tilapia (Oreochromis niloticus) model, we investigated the suppression of T cell response by CTLA-4 in bony fish. Tilapia CTLA-4 is constitutively expressed in lymphoid tis...
Source: Journal of Immunology - February 16, 2024 Category: Allergy & Immunology Authors: Jiansong Zhang Xiumei Wei Qian Zhang Xinying Jiao Kang Li Ming Geng Yi Cao Ding Wang Jie Cheng Jialong Yang Source Type: research
Cutting Edge: The Tetraspanin CD53 Promotes CXCR4 Signaling and Bone Marrow Homing in B Cells
In this study, we identified the tetraspanin CD53 as a critical regulator of both normal and malignant B cell trafficking. CXCL12 is a key chemokine in B cell homing to the bone marrow and secondary lymphoid organs, and both normal and malignant B cells from Cd53-/- mice have reduced migration toward CXCL12 in vitro, as well as impaired marrow homing in vivo. Using proximity ligation studies, we identified the CXCL12 receptor, CXCR4, as a novel, to our knowledge, CD53 binding partner. This interaction promotes receptor function, because Cd53-/- B cells display reduced signaling and internalization of CXCR4 in response to C...
Source: Journal of Immunology - February 16, 2024 Category: Allergy & Immunology Authors: Mousumi Chakraborty Zev J Greenberg Qian Dong Nate Roundy Jeffrey J Bednarski Luana Chiquetto Paracatu Eric Duncavage Weikai Li Laura G Schuettpelz Source Type: research
Cytotoxic Programming of CD4+ T Cells Is Regulated by Opposing Actions of the Related Transcription Factors Eos and Aiolos
J Immunol. 2024 Feb 16:ji2300748. doi: 10.4049/jimmunol.2300748. Online ahead of print.ABSTRACTIn contrast to the "helper" activities of most CD4+ T effector subsets, CD4+ cytotoxic T lymphocytes (CD4-CTLs) perform functions normally associated with CD8+ T and NK cells. Specifically, CD4-CTLs secrete cytotoxic molecules and directly target and kill compromised cells in an MHC class II-restricted fashion. The functions of these cells have been described in diverse immunological contexts, including their ability to provide protection during antiviral and antitumor responses, as well as being implicated in autoimmunity. Despi...
Source: Journal of Immunology - February 16, 2024 Category: Allergy & Immunology Authors: Devin M Jones Jasmine A Tuazon Kaitlin A Read Melissa R Leonard Srijana Pokhrel Bharath K Sreekumar Robert T Warren Jacob S Yount Patrick L Collins Kenneth J Oestreich Source Type: research
Fish Uses CTLA-4 Immune Checkpoint to Suppress mTORC1-Controlled T-Cell Glycolysis and Immunity
J Immunol. 2024 Feb 16:ji2300599. doi: 10.4049/jimmunol.2300599. Online ahead of print.ABSTRACTAs an immune checkpoint, cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) suppresses the activation, proliferation, and effector function of T cells, thus preventing an overexuberant response and maintaining immune homeostasis. However, whether and how this immune checkpoint functions in early vertebrates remains unknown. In the current study, using a Nile tilapia (Oreochromis niloticus) model, we investigated the suppression of T cell response by CTLA-4 in bony fish. Tilapia CTLA-4 is constitutively expressed in lymphoid tis...
Source: Journal of Immunology - February 16, 2024 Category: Allergy & Immunology Authors: Jiansong Zhang Xiumei Wei Qian Zhang Xinying Jiao Kang Li Ming Geng Yi Cao Ding Wang Jie Cheng Jialong Yang Source Type: research
Cutting Edge: The Tetraspanin CD53 Promotes CXCR4 Signaling and Bone Marrow Homing in B Cells
In this study, we identified the tetraspanin CD53 as a critical regulator of both normal and malignant B cell trafficking. CXCL12 is a key chemokine in B cell homing to the bone marrow and secondary lymphoid organs, and both normal and malignant B cells from Cd53-/- mice have reduced migration toward CXCL12 in vitro, as well as impaired marrow homing in vivo. Using proximity ligation studies, we identified the CXCL12 receptor, CXCR4, as a novel, to our knowledge, CD53 binding partner. This interaction promotes receptor function, because Cd53-/- B cells display reduced signaling and internalization of CXCR4 in response to C...
Source: Journal of Immunology - February 16, 2024 Category: Allergy & Immunology Authors: Mousumi Chakraborty Zev J Greenberg Qian Dong Nate Roundy Jeffrey J Bednarski Luana Chiquetto Paracatu Eric Duncavage Weikai Li Laura G Schuettpelz Source Type: research
