Alternatively Spliced Variants of Murine CD247 Influence T Cell Development and Activation, Revealing the Importance of the CD3 ζ C-Terminal Region
In this study, we used a retrovirus-mediated gene transfer technique to express these three isoforms individually and examined the roles of them on T cell development and activation. Rag1-/- mice reconstituted with CD3θ-expressing bone marrow failed to develop mature T cells. CD3ι-expressing T cells exhibited similar development and activation as cells expressing CD3ζ. In contrast, thymic development was severely impaired in CD3η-reconstituted mice. Single-positive but not double-positive CD3η-expressing thymocytes had reduced TCR expression, and CD5 expression was decreased at the double-positive stage, suggesting a ...
Source: Journal of Immunology - December 20, 2023 Category: Allergy & Immunology Authors: Ye Jin Huijuan Yuan Isha Mehta Ogechukwu Ezenwa Penelope A Morel Source Type: research
Cutting Edge: PDGF-DD Binding to NKp44 Costimulates TLR9 Signaling and Proinflammatory Cytokine Secretion in Human Plasmacytoid Dendritic Cells
J Immunol. 2023 Dec 20:ji2200496. doi: 10.4049/jimmunol.2200496. Online ahead of print.ABSTRACTNKp44 is a human receptor originally found on activated NK cells, group 1 and group 3 innate lymphoid cells that binds dimers of platelet-derived growth factor D (PDGF-DD). NKp44 is also expressed on tissue plasmacytoid dendritic cells (PDCs), but NKp44-PDGF-DD interaction on PDCs remains unstudied. Engagement of NKp44 with PDGF-DD in vitro enhanced PDC secretion of IFN-α, TNF, and IL-6 in response to the TLR9 ligand CpG-ODN, but not TLR7/8 ligands. In tissues, PDCs were found in close contact with PDGF-DD-expressing cells in th...
Source: Journal of Immunology - December 20, 2023 Category: Allergy & Immunology Authors: Alexander David Barrow Marina Cella Melissa Anne Edeling Md Abdullah-Al-Kamran Khan Luisa Cervantes-Barragan Mattia Bugatti Christian Schmedt William Vermi Marco Colonna Source Type: research
A Bacterial and Ganglioside-based Nanoparticle Initiates Reprogramming of Macrophages and Promotes Antitumor Phenotypes
In this study, in mouse models, we focused on macrophages and tested the hypothesis that VSSP drives macrophages toward M1-like functional states via IRF8- and PU.1-dependent mechanisms. We further hypothesized that such VSSP-mediated actions would be accompanied by enhanced antitumor responses. Overall, we showed that (1) VSSP drives naive or M2-derived macrophages to M1-like states, (2) the M1-like state induced by VSSP occurs via IRF8- and PU.1-dependent mechanisms, and (3) single-agent VSSP induces an antitumor response that is accompanied by alterations in the intratumoral myeloid compartment. These results provide a ...
Source: Journal of Immunology - December 20, 2023 Category: Allergy & Immunology Authors: Rydell Alvarez-Arzola Liliana Oliver Michelle M Messmer Danielle Y F Twum Kelvin P Lee Jason B Muhitch Circe Mesa Scott I Abrams Source Type: research
Modulating the sEH/EETs Axis Restrains Specialized Proresolving Mediator Impairment and Regulates T Cell Imbalance in Experimental Periodontitis
J Immunol. 2023 Dec 20:ji2300650. doi: 10.4049/jimmunol.2300650. Online ahead of print.ABSTRACTEpoxyeicosatrienoic acids (EETs) and other epoxy fatty acids are short-acting lipids involved in resolution of inflammation. Their short half-life, due to its metabolism by soluble epoxide hydrolase (sEH), limits their effects. Specialized proresolving mediators (SPMs) are endogenous regulatory lipids insufficiently synthesized in uncontrolled and chronic inflammation. Using an experimental periodontitis model, we pharmacologically inhibited sEH, examining its impact on T cell activation and systemic SPM production. In humans, we...
Source: Journal of Immunology - December 20, 2023 Category: Allergy & Immunology Authors: Henrique B Abdalla Luciano Puhl Carla Alvarez Rivas Yu-Chiao Wu Paola Rojas Carlos Antonio Trindade-da-Silva Bruce D Hammock Krishna R Maddipati Mariana Q S Soares Juliana T Clemente-Napimoga Alpdogan Kantarci Marcelo H Napimoga Thomas E Van Dyke Source Type: research
Dynamic MAIT Cell Recovery after Severe COVID-19 Is Transient with Signs of Heterogeneous Functional Anomalies
J Immunol. 2023 Dec 20:ji2300639. doi: 10.4049/jimmunol.2300639. Online ahead of print.ABSTRACTMucosal-associated invariant T (MAIT) cells are an abundant population of unconventional T cells in humans and play important roles in immune defense against microbial infections. Severe COVID-19 is associated with strong activation of MAIT cells and loss of these cells from circulation. In the present study, we investigated the capacity of MAIT cells to recover after severe COVID-19. In longitudinal paired analysis, MAIT cells initially rebounded numerically and phenotypically in most patients at 4 mo postrelease from the hospit...
Source: Journal of Immunology - December 20, 2023 Category: Allergy & Immunology Authors: Tobias Kammann Jean-Baptiste Gorin Tiphaine Parrot Yu Gao Andrea Ponzetta Johanna Emg ård Kimia T Maleki Takuya Sekine Olga Rivera-Ballesteros Karolinska COVID-19 Study Group Sara Gredmark-Russ Olav Rooyackers Magdalena Skagerberg Lars I Eriksson Anna No Source Type: research