Antiviral and Anti-Inflammatory Therapeutic Effect of RAGE-Ig Protein against Multiple SARS-CoV-2 Variants of Concern Demonstrated in K18-hACE2 Mouse and Syrian Golden Hamster Models
J Immunol. 2024 Jan 5:ji2300392. doi: 10.4049/jimmunol.2300392. Online ahead of print.ABSTRACTSARS-CoV-2 variants of concern (VOCs) continue to evolve and reemerge with chronic inflammatory long COVID sequelae, necessitating the development of anti-inflammatory therapeutic molecules. Therapeutic effects of the receptor for advanced glycation end products (RAGE) were reported in many inflammatory diseases. However, a therapeutic effect of RAGE in COVID-19 has not been reported. In the present study, we investigated whether and how the RAGE-Ig fusion protein would have an antiviral and anti-inflammatory therapeutic effect in...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Nisha Rajeswari Dhanushkodi Swayam Prakash Afshana Quadiri Latifa Zayou Ruchi Srivastava Amin Mohammed Shaik Berfin Suzer Izabela Coimbra Ibraim Gary Landucci Delia F Tifrea Mahmoud Singer Leila Jamal Robert A Edwards Hawa Vahed Lawrence Brown Lbachir Ben Source Type: research
CL-K1 Promotes Complement Activation and Regulates Opsonophagocytosis of Macrophages with CD93 Interaction in a Primitive Vertebrate
In this study, the Oreochromis niloticus CL-K1 (OnCL-K1) protein was purified and identified, which was capable of binding to two important pathogens of tilapia, Streptococcus agalactiae and Aeromonas hydrophila. Interestingly, OnCL-K1 exhibited direct bactericidal activity by binding to lipoteichoic acid or LPS on cell walls, disrupting the permeability and integrity of the bacterial membrane in vitro. Upon bacterial challenge, OnCL-K1 significantly inhibited the proliferation of pathogenic bacteria, reduced the inflammatory response, and improved the survival of tilapia. Further research revealed that OnCL-K1 could assoc...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Liangliang Mu Xiaoxue Yin Li Qiu Jiadong Li Jinfen Mo Hao Bai Qingliang Zeng Jianmin Ye Source Type: research
Recruitment and Maintenance of CX3CR1+CD4+ T Cells during Helminth Infection
In this study, we used a fate-mapping mouse model to characterize CX3CR1+CD4+ T cells during both acute Nippostrongylus brasiliensis and chronic Schistosoma mansoni murine models of helminth infections, revealing CX3CR1+CD4+ T cells to be an activated tissue-homing subset with varying capacity for cytokine production. Tracking these cells over time revealed that maintenance of CX3CR1 itself along with a TH2 phenotype conferred a survival advantage in the inflamed tissue. Single-cell RNA sequencing analysis of fate-mapped CX3CR1+CD4+ T cells from both the peripheral tissue and the spleen revealed a considerable level of div...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Denis G Loredan Joseph C Devlin Kamal M Khanna P'ng Loke Source Type: research
Antiviral and Anti-Inflammatory Therapeutic Effect of RAGE-Ig Protein against Multiple SARS-CoV-2 Variants of Concern Demonstrated in K18-hACE2 Mouse and Syrian Golden Hamster Models
J Immunol. 2024 Jan 5:ji2300392. doi: 10.4049/jimmunol.2300392. Online ahead of print.ABSTRACTSARS-CoV-2 variants of concern (VOCs) continue to evolve and reemerge with chronic inflammatory long COVID sequelae, necessitating the development of anti-inflammatory therapeutic molecules. Therapeutic effects of the receptor for advanced glycation end products (RAGE) were reported in many inflammatory diseases. However, a therapeutic effect of RAGE in COVID-19 has not been reported. In the present study, we investigated whether and how the RAGE-Ig fusion protein would have an antiviral and anti-inflammatory therapeutic effect in...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Nisha Rajeswari Dhanushkodi Swayam Prakash Afshana Quadiri Latifa Zayou Ruchi Srivastava Amin Mohammed Shaik Berfin Suzer Izabela Coimbra Ibraim Gary Landucci Delia F Tifrea Mahmoud Singer Leila Jamal Robert A Edwards Hawa Vahed Lawrence Brown Lbachir Ben Source Type: research
CL-K1 Promotes Complement Activation and Regulates Opsonophagocytosis of Macrophages with CD93 Interaction in a Primitive Vertebrate
In this study, the Oreochromis niloticus CL-K1 (OnCL-K1) protein was purified and identified, which was capable of binding to two important pathogens of tilapia, Streptococcus agalactiae and Aeromonas hydrophila. Interestingly, OnCL-K1 exhibited direct bactericidal activity by binding to lipoteichoic acid or LPS on cell walls, disrupting the permeability and integrity of the bacterial membrane in vitro. Upon bacterial challenge, OnCL-K1 significantly inhibited the proliferation of pathogenic bacteria, reduced the inflammatory response, and improved the survival of tilapia. Further research revealed that OnCL-K1 could assoc...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Liangliang Mu Xiaoxue Yin Li Qiu Jiadong Li Jinfen Mo Hao Bai Qingliang Zeng Jianmin Ye Source Type: research
Recruitment and Maintenance of CX3CR1+CD4+ T Cells during Helminth Infection
In this study, we used a fate-mapping mouse model to characterize CX3CR1+CD4+ T cells during both acute Nippostrongylus brasiliensis and chronic Schistosoma mansoni murine models of helminth infections, revealing CX3CR1+CD4+ T cells to be an activated tissue-homing subset with varying capacity for cytokine production. Tracking these cells over time revealed that maintenance of CX3CR1 itself along with a TH2 phenotype conferred a survival advantage in the inflamed tissue. Single-cell RNA sequencing analysis of fate-mapped CX3CR1+CD4+ T cells from both the peripheral tissue and the spleen revealed a considerable level of div...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Denis G Loredan Joseph C Devlin Kamal M Khanna P'ng Loke Source Type: research
Antiviral and Anti-Inflammatory Therapeutic Effect of RAGE-Ig Protein against Multiple SARS-CoV-2 Variants of Concern Demonstrated in K18-hACE2 Mouse and Syrian Golden Hamster Models
J Immunol. 2024 Jan 5:ji2300392. doi: 10.4049/jimmunol.2300392. Online ahead of print.ABSTRACTSARS-CoV-2 variants of concern (VOCs) continue to evolve and reemerge with chronic inflammatory long COVID sequelae, necessitating the development of anti-inflammatory therapeutic molecules. Therapeutic effects of the receptor for advanced glycation end products (RAGE) were reported in many inflammatory diseases. However, a therapeutic effect of RAGE in COVID-19 has not been reported. In the present study, we investigated whether and how the RAGE-Ig fusion protein would have an antiviral and anti-inflammatory therapeutic effect in...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Nisha Rajeswari Dhanushkodi Swayam Prakash Afshana Quadiri Latifa Zayou Ruchi Srivastava Amin Mohammed Shaik Berfin Suzer Izabela Coimbra Ibraim Gary Landucci Delia F Tifrea Mahmoud Singer Leila Jamal Robert A Edwards Hawa Vahed Lawrence Brown Lbachir Ben Source Type: research
CL-K1 Promotes Complement Activation and Regulates Opsonophagocytosis of Macrophages with CD93 Interaction in a Primitive Vertebrate
In this study, the Oreochromis niloticus CL-K1 (OnCL-K1) protein was purified and identified, which was capable of binding to two important pathogens of tilapia, Streptococcus agalactiae and Aeromonas hydrophila. Interestingly, OnCL-K1 exhibited direct bactericidal activity by binding to lipoteichoic acid or LPS on cell walls, disrupting the permeability and integrity of the bacterial membrane in vitro. Upon bacterial challenge, OnCL-K1 significantly inhibited the proliferation of pathogenic bacteria, reduced the inflammatory response, and improved the survival of tilapia. Further research revealed that OnCL-K1 could assoc...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Liangliang Mu Xiaoxue Yin Li Qiu Jiadong Li Jinfen Mo Hao Bai Qingliang Zeng Jianmin Ye Source Type: research
Recruitment and Maintenance of CX3CR1+CD4+ T Cells during Helminth Infection
In this study, we used a fate-mapping mouse model to characterize CX3CR1+CD4+ T cells during both acute Nippostrongylus brasiliensis and chronic Schistosoma mansoni murine models of helminth infections, revealing CX3CR1+CD4+ T cells to be an activated tissue-homing subset with varying capacity for cytokine production. Tracking these cells over time revealed that maintenance of CX3CR1 itself along with a TH2 phenotype conferred a survival advantage in the inflamed tissue. Single-cell RNA sequencing analysis of fate-mapped CX3CR1+CD4+ T cells from both the peripheral tissue and the spleen revealed a considerable level of div...
Source: Journal of Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Denis G Loredan Joseph C Devlin Kamal M Khanna P'ng Loke Source Type: research
Schistosome Infection Impacts Hematopoiesis
J Immunol. 2024 Jan 3:ji2300195. doi: 10.4049/jimmunol.2300195. Online ahead of print.ABSTRACTHelminth infections are common in animals. However, the impact of a helminth infection on the function of hematopoietic stem cells (HSCs) and other hematopoietic cells has not been comprehensively defined. In this article, we describe the hematopoietic response to infection of mice with Schistosoma mansoni, a parasitic flatworm that causes schistosomiasis. We analyzed the frequency or number of hematopoietic cell types in the bone marrow, spleen, liver, thymus, and blood and observed multiple hematopoietic changes caused by infect...
Source: Journal of Immunology - January 3, 2024 Category: Allergy & Immunology Authors: Tobias Wijshake Joseph Rose Jipeng Wang Jacob Zielke Madeleine Marlar-Pavey Weina Chen James J Collins Michalis Agathocleous Source Type: research
Corrections: A2B adenosine receptor expression by myeloid cells is pro-inflammatory in murine allergic-airway inflammation
J Immunol. 2024 Jan 3:ji2300855. doi: 10.4049/jimmunol.2300855. Online ahead of print.ABSTRACTBelikoff, B. G., L. J. Vaickus, M. Sitkovsky, and D. G. Remick. 2012. A2B adenosine receptor expression by myeloid cells is pro-inflammatory in murine allergic-airway inflammation. J. Immunol. 189: 3707-3713. Two of the panels in Fig. 2C (the histology photomicrographs for wild type and A2B receptor knockout) were inadvertently duplicated. Due to the age of the article, the authors no longer have the original raw data for these panels to correct the figure. However, these photomicrographs were meant to be representative histologic...
Source: Journal of Immunology - January 3, 2024 Category: Allergy & Immunology Authors: Bryan G Belikoff Louis J Vaickus Michail Sitkovsky Daniel G Remick Source Type: research
Histone Deacetylase Inhibitors Directly Modulate T Cell Gene Expression and Signaling and Promote Development of Effector-Exhausted T Cells in Murine Tumors
J Immunol. 2024 Jan 3:ji2300475. doi: 10.4049/jimmunol.2300475. Online ahead of print.ABSTRACTEpigenetic regulation plays a crucial role in the development and progression of cancer, including the regulation of antitumor immunity. The reversible nature of epigenetic modifications offers potential therapeutic avenues for cancer treatment. In particular, histone deacetylase (HDAC) inhibitors (HDACis) have been shown to promote antitumor T cell immunity by regulating myeloid cell types, enhancing tumor Ag presentation, and increasing expression of chemokines. HDACis are currently being evaluated to determine whether they can ...
Source: Journal of Immunology - January 3, 2024 Category: Allergy & Immunology Authors: Mohammed L Ibrahim Hong Zheng Margaret L Barlow Yousuf Latif Zhihua Chen Xiaoqing Yu Amer A Beg Source Type: research
A Lipopolysaccharide-Enriched Cow's Milk Allergy Microbiome Promotes a TLR4-Dependent Proinflammatory Intestinal Immune Response
In this study we selected a CMA-associated microbiota with increased abundance of Gram-negative bacteria for analysis of its proinflammatory potential. LPS is the major component of the outer membrane of Gram-negative bacteria. Colonization of mice with a global or conditional mutation of the LPS receptor TLR4 with this CMA microbiota induced expression of serum amyloid A1 (Saa1) and other Th17-, B cell-, and Th2-associated genes in the ileal epithelium in a TLR4-dependent manner. In agreement with the gene expression data, mice colonized with the CMA microbiota have expanded populations of Th17 and regulatory T cells and ...
Source: Journal of Immunology - January 3, 2024 Category: Allergy & Immunology Authors: Evelyn Campbell Lauren A Hesser Roberto Berni Canani Laura Carucci Lorella Paparo Robert T Patry Cathryn R Nagler Source Type: research
Schistosome Infection Impacts Hematopoiesis
J Immunol. 2024 Jan 3:ji2300195. doi: 10.4049/jimmunol.2300195. Online ahead of print.ABSTRACTHelminth infections are common in animals. However, the impact of a helminth infection on the function of hematopoietic stem cells (HSCs) and other hematopoietic cells has not been comprehensively defined. In this article, we describe the hematopoietic response to infection of mice with Schistosoma mansoni, a parasitic flatworm that causes schistosomiasis. We analyzed the frequency or number of hematopoietic cell types in the bone marrow, spleen, liver, thymus, and blood and observed multiple hematopoietic changes caused by infect...
Source: Journal of Immunology - January 3, 2024 Category: Allergy & Immunology Authors: Tobias Wijshake Joseph Rose Jipeng Wang Jacob Zielke Madeleine Marlar-Pavey Weina Chen James J Collins Michalis Agathocleous Source Type: research
Corrections: A2B adenosine receptor expression by myeloid cells is pro-inflammatory in murine allergic-airway inflammation
J Immunol. 2024 Jan 3:ji2300855. doi: 10.4049/jimmunol.2300855. Online ahead of print.ABSTRACTBelikoff, B. G., L. J. Vaickus, M. Sitkovsky, and D. G. Remick. 2012. A2B adenosine receptor expression by myeloid cells is pro-inflammatory in murine allergic-airway inflammation. J. Immunol. 189: 3707-3713. Two of the panels in Fig. 2C (the histology photomicrographs for wild type and A2B receptor knockout) were inadvertently duplicated. Due to the age of the article, the authors no longer have the original raw data for these panels to correct the figure. However, these photomicrographs were meant to be representative histologic...
Source: Journal of Immunology - January 3, 2024 Category: Allergy & Immunology Authors: Bryan G Belikoff Louis J Vaickus Michail Sitkovsky Daniel G Remick Source Type: research
