The mechanism of lncRNA SNHG1 in osteogenic differentiation via miR-497-5p/ HIF1AN axis
Connect Tissue Res. 2023 Nov 15:1-10. doi: 10.1080/03008207.2023.2281321. Online ahead of print.ABSTRACTThe pivotal role of lncRNAs in osteoporosis progression and development necessitates a comprehensive exploration of the functional and precise molecular mechanisms underlying lncRNA SNHG1's regulation of osteoblast differentiation and calcification. The study involved inducing BMSCs cells to differentiate into osteoblasts, followed by transfections of miR-497-5p inhibitors, pcDNA3.1-SNHG1, sh-HIF1AN, miR-497-5p mimics, and respective negative controls into BMSCs. Quantitative PCR (qPCR) was employed to assess the express...
Source: Connective Tissue Research - November 15, 2023 Category: Research Authors: Yuanyuan Lu Kaihua Pan Yunqing Zhang Jiang Peng Daning Cao Xiaoming Li Source Type: research
Acidosis induces significant changes to the murine supraspinatus enthesis organic matrix
Connect Tissue Res. 2023 Nov 14:1-12. doi: 10.1080/03008207.2023.2275044. Online ahead of print.ABSTRACTRotator cuff pathology is a common musculoskeletal condition that disproportionately affects older adults, as well as patients with diabetes mellitus and chronic kidney disease. It is known that increased age and kidney dysfunction have been correlated to acidotic states, which may be related to the increased incidence of rotator cuff injury. In order to investigate the potential relationship between acidosis and rotator cuff composition and mechanics, this study utilizes a 14-day murine model of metabolic acidosis and e...
Source: Connective Tissue Research - November 14, 2023 Category: Research Authors: Saparja Nag Isabelle De Bruyker Ashley Nelson Mikayla Moody Marla Fais Alix C Deymier Source Type: research
TLR4 antagonism provides short-term but not long-term clinical benefit in a full-depth cartilage defect mouse model
CONCLUSIONS: Collectively, this work demonstrates that systemic treatment with a TLR4 antagonist is protective to further cartilage damage 14-days post-injury in a murine model of induced disease.PMID:37898909 | DOI:10.1080/03008207.2023.2269257 (Source: Connective Tissue Research)
Source: Connective Tissue Research - October 29, 2023 Category: Research Authors: Ariel E Timkovich G Aaron Holling Maryam F Afzali John Kisiday Kelly S Santangelo Source Type: research
