Contributors
MILENKO JOVAN TANASIJEVIC, MD, MBA (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - July 21, 2023 Category: Laboratory Medicine Source Type: research
Contents
Christopher B. Hergott (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - July 21, 2023 Category: Laboratory Medicine Source Type: research
Forthcoming Issues
Myelodysplastic Syndromes (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - July 21, 2023 Category: Laboratory Medicine Source Type: research
A Review of the Flow Cytometric Findings in Classic Hodgkin Lymphoma, Nodular Lymphocyte Predominant Hodgkin Lymphoma and T Cell/Histiocyte-Rich Large B Cell Lymphoma
Classic Hodgkin lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, and T cell/histiocyte-rich large B cell lymphoma form a unique set of lymphomas with similar morphologic growth patterns (occasional neoplastic cells within a prominent cellular cell background) that are pathobiologically related. Distinguishing these entities has been historically difficult by flow cyt ometry; however, our laboratory has developed antibody-fluorochrome combinations capable of immunophenotyping these lymphomas. Additionally, characterization of the background reactive lymphocytes can aid in narrowing the differential diagnosis. Th...
Source: Clinics in Laboratory Medicine - June 23, 2023 Category: Laboratory Medicine Authors: Feras Ally, David Gajzer, Jonathan R. Fromm Source Type: research
Advances in Clinical Mass Cytometry
This article introduces CyTOF-based assays to profile immune cell-types, cell-states, and their applications in clinical research. (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - June 20, 2023 Category: Laboratory Medicine Authors: Abhishek Koladiya, Kara L. Davis Source Type: research
Flow Cytometry in Diagnosis, Prognostication, and Monitoring of Multiple Myeloma and Related Disorders
Flow cytometry plays a critical role in the diagnosis, prognostication, therapy response evaluation, and clinical management of plasma cell neoplasms. The review summarizes how flow cytometry is used in the initial evaluation to distinguish primary and secondary clonal plasma cell populations from each other and from reactive plasma cells. We further illustrate the kinds of prognostic information the assessment can provide at diagnosis and disease follow-up of primary plasma cell neoplasms. Technical requirements for MRD assays and their use in therapy efficacy assessment and clinical decision-making in multi-myeloma are d...
Source: Clinics in Laboratory Medicine - June 17, 2023 Category: Laboratory Medicine Authors: Mikhail Roshal, Qi Gao Source Type: research
Advances in Acute Myeloid Leukemia Classification, Prognostication and Monitoring by Flow Cytometry
Although final classification of acute myeloid leukemia (AML) integrates morphologic, cytogenetic, and molecular data, flow cytometry remains an essential component of modern AML diagnostics. Here, we review the current role of flow cytometry in the classification, prognostication, and monitoring of AML. We cover immunophenotypic features of key genetically defined AML subtypes and their effects on biological and clinical behaviors, review clinically tractable strategies to differentiate leukemias with ambiguous immunophenotypes more accurately and discuss key principles of standardization for measurable residual disease m...
Source: Clinics in Laboratory Medicine - June 11, 2023 Category: Laboratory Medicine Authors: Fabienne Lucas, Christopher B. Hergott Source Type: research
Advances in Flow Cytometry for Mixed Phenotype and Ambiguous Leukemias
This review discusses recent updates in the diagnosis of acute leukemias of ambiguous lineage and emphasizes the necessary elements for proper flow cytometric evaluation of these cases. The current emphasis of the classification system is toward interpreting the marker expression in light of the intensity of lineage markers and avoiding a diagnosis of mixed phenotype acute leukemia based solely on immunophenotyping without considering underlying genetic findings. Novel entities including mixed phenotype acute leukemia with ZNF384 rearrangements and acute leukemias of ambiguous lineage with BCL11B rearrangements seem to sho...
Source: Clinics in Laboratory Medicine - June 9, 2023 Category: Laboratory Medicine Authors: Jason H. Kurzer, Olga K. Weinberg Source Type: research
Utility of Flow Cytometry Analysis in the Detection of Nonhematologic Neoplasms
Flow cytometry analysis has stood the test of time as a powerful tool in the assessment of hematologic neoplasms. The role of flow cytometry has expanded to evaluate various nonhematologic neoplasms encountered in body cavity malignant effusions, lymph nodes, and other body sites. This review explores the use of routine antibody panels as well as specially designed multicolor antibody panels that have been investigated by different groups and reported in the literature for evaluating nonhematologic neoplasms. In this context, the limitations, pitfalls, future directions, and promising applications of flow cytometry analysi...
Source: Clinics in Laboratory Medicine - June 9, 2023 Category: Laboratory Medicine Authors: Hibbah Nabeel, Bachir Alobeid Source Type: research
Evolving Approach to Clinical Cytometry for Immunodeficiencies and Other Immune Disorders
Primary immunodeficiencies were initially identified on the basis of recurrent, severe or unusual infections. Subsequently, it was noted that these diseases can also manifest with autoimmunity, autoinflammation, allergy, lymphoproliferation and malignancy, hence a conceptual change and their renaming as inborn errors of immunity. Ongoing advances in flow cytometry provide the opportunity to expand or modify the utility and scope of existing laboratory tests in this field to mirror this conceptual change. Here we have used the B cell subset, variably known as CD21low B cells, age-associated B cells and T-bet+ B cells, as...
Source: Clinics in Laboratory Medicine - June 9, 2023 Category: Laboratory Medicine Authors: Amir A. Sadighi Akha, Kriszti án Csomós, Boglárka Ujházi, Jolán E. Walter, Attila Kumánovics Source Type: research
The Immunophenotypic Profile of Healthy Human Bone Marrow
Flow cytometry enables multiparametric characterization of hematopoietic cell immunophenotype. Deviations from normal immunophenotypic patterns comprise a cardinal feature of many hematopoietic neoplasms, underscoring the ongoing essentiality of flow cytometry as a diagnostic tool. However, understanding of aberrant hematopoiesis requires an equal understanding of normal hematopoiesis as a comparator. In this review, we outline key features of healthy adult hematopoiesis and lineage specification as illuminated by flow cytometry and provide diagrams illustrating what a diagnostician may observe in flow cytometric plots. Th...
Source: Clinics in Laboratory Medicine - June 4, 2023 Category: Laboratory Medicine Authors: Joshua E. Lewis, Christopher B. Hergott Source Type: research
Clinical Flow Cytometry Analysis in the Setting of Chronic Myeloid Neoplasms and Clonal Hematopoiesis
The utility of flow cytometry analysis in the evaluation of chronic myeloid neoplasms, such as myelodysplastic neoplasms and chronic myeloproliferative neoplasms, continues to be emphasized and explored. Recently flow cytometry analysis has been also proven to be able to distinguish persistent clonal hematopoiesis from measurable residual disease in patients with acute myeloid leukemia (AML), a finding with potential critical treatment impact in the management of patients with AML. (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - June 1, 2023 Category: Laboratory Medicine Authors: Siba El Hussein, Sanam Loghavi Source Type: research
Advances in Monitoring and Prognostication for Lymphoma by Flow Cytometry
Flow cytometry (FC) is a well-established method important in the diagnosis and subclassification of lymphoma. In this article, the role of FC in lymphoma prognostication will be explored, and the clinical role for FC minimal/measurable residual disease testing as a monitoring tool for mature lymphoma will be introduced. Potential pitfalls of monitoring for residual/recurrent disease following immunotherapy will be presented. (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - June 1, 2023 Category: Laboratory Medicine Authors: Amrit P. Singh, Elizabeth L. Courville Source Type: research
How to Design and Validate a Clinical Flow Cytometry Assay
Multiparametric flow cytometry assays are long recognized as an essential diagnostic test for leukemias and lymphomas. Lacking Food and Drug Administration –approved standardized tests, these assays remain laboratory developed tests. The recently published guidelines, CLSI H62, are the most detailed and up-to-date instructions for designing and validating clinical flow cytometry assays. This review provides a historical background for the current sit uation, summarizes key points from the CLSI guidelines, and lists practical points for assay development gained from personal experience. (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - May 29, 2023 Category: Laboratory Medicine Authors: Anand Shreeram Lagoo Source Type: research
Clinical Cytometry for Platelets and Platelet Disorders
Clinical flow cytometry tests for inherited and acquired platelet disorders are useful diagnostic tools but are not widely available. Flow cytometric methods are available to detect inherited glycoprotein deficiencies, granule release (secretion defects), drug-induced thrombocytopenias, presence of antiplatelet antibodies, and pharmacodynamic inhibition by antiplatelet agents. New tests take advantage of advanced multicolor cytometers and allow identification of novel platelet subsets by high-dimensional immunophenotyping. Studies are needed to evaluate the value of these new tests for diagnosis and monitoring of therapy i...
Source: Clinics in Laboratory Medicine - May 29, 2023 Category: Laboratory Medicine Authors: Andrew L. Frelinger, Benjamin E.J. Spurgeon Source Type: research
