Matrix-Assisted Laser Desorption Ionization Time-of-Flight for Fungal Identification
Many studies have shown successful performance of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for rapid yeast and mold identification, yet few laboratories have chosen to apply this technology into their routine clinical mycology workflow. This review provides an overview of the current status of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for fungal identification, including key findings in the literature, processing and database considerations, updates in technology, and exciting future prospects. Significant advances toward standardization have taken plac...
Source: Clinics in Laboratory Medicine - April 24, 2021 Category: Laboratory Medicine Authors: Anna F. Lau Source Type: research
Mass Spectrometry and Microbial Diagnostics in the Clinical Laboratory
Over the past decade, matrix-assisted laser desorption/ionization –time-of-flight mass spectrometry has revolutionized the practice of clinical microbiology and infectious disease diagnostics. Rapid advancement has occurred through the development and implementation of mass spectrometric protein profiling technologies that are widely available. Ease of sample pr eparation, rapid turnaround times, and high throughput accuracy have accelerated acceptance within the clinical laboratory. New mass spectrometric technologies centered on multiple microbial diagnostic markers are in development. Such new applications, reviewed i...
Source: Clinics in Laboratory Medicine - April 24, 2021 Category: Laboratory Medicine Authors: Christopher R. Cox, Rebecca M. Harris Source Type: research
Mass Cytometry, Imaging Mass Cytometry, and Multiplexed Ion Beam Imaging Use in a Clinical Setting
Mass cytometry (MC), imaging mass cytometry (IMC), and multiplexed ion beam imaging (MIBI) represent a new generation of tools to understand increasingly complex systems. Although these technologies differ in their intended applications, with MC being most similar to flow cytometry, and IMC/MIBI being similar to immunohistochemistry, they all share a time of flight mass spectrometry (TOF MS) platform. These TOF MS platforms use metal conjugated antibodies as opposed to fluorophores, increasing the measurable parameters up to approximately 50 with a theoretic limit approximately 100 parameters. These tools are being adapted...
Source: Clinics in Laboratory Medicine - April 24, 2021 Category: Laboratory Medicine Authors: Raymond D. Devine, Gregory K. Behbehani Source Type: research
Transfusion in Pediatric Patients
This article r eviews existing literature on pediatric transfusion therapy within the larger context of analogous data in adult populations. (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - January 23, 2021 Category: Laboratory Medicine Authors: Yunchuan Delores Mo, Meghan Delaney Source Type: research
Cellular Therapy in Pediatric Hematologic Malignancies
This article reviews current cellular therapies in pediatric hematological malignancy, including the most updated practices in hematopoietic stem cell transplant and the use of chimeric antigen receptor (CAR) therapy in T cells. Hematopoietic stem cell transplant has evolved with improvements in chemotherapy regimens, immunosuppression, and donor-matching options. Novel therapies in development which will likely further improve the options for patients are reviewed including Natural Killer, Regulatory T-cells and αβ depletion. (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - January 23, 2021 Category: Laboratory Medicine Authors: Susan Kuldanek, Bryce Pasko, Melkon DomBourian, Kyle Annen Source Type: research
Topics in Pediatric Transfusion Medicine
CLINICS IN LABORATORY MEDICINE (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - January 23, 2021 Category: Laboratory Medicine Authors: Sarah R. Vossoughi, Brie A. Stotler Source Type: research
Copyright
Elsevier (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - January 23, 2021 Category: Laboratory Medicine Source Type: research
Contributors
MILENKO JOVAN TANASIJEVIC, MD, MBA (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - January 23, 2021 Category: Laboratory Medicine Source Type: research
Contents
Sarah R. Vossoughi and Brie A. Stotler (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - January 23, 2021 Category: Laboratory Medicine Source Type: research
Forthcoming Issues
Beyond LC MS: The Next Frontier in Clinical Mass Spectrometry (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - January 23, 2021 Category: Laboratory Medicine Source Type: research
Inventory Management and Product Selection in Pediatric Blood Banking
Blood banks need to understand patterns of use and ordering practices to provide the blood donor centers with the best information with which to develop daily scheduled deliveries of blood products. Blood use is a large component of this process through maximizing physician education about appropriate ordering practices and use of appropriate tools. Simple measures can help provide guidance on the number of available components and the need to order more from the blood donor center. Special product requests in pediatrics, such as fresh blood, leukoreduction, irradiation, and antigen-negative units can also drive inventory ...
Source: Clinics in Laboratory Medicine - December 23, 2020 Category: Laboratory Medicine Authors: Jenna T. Reece, Deborah Sesok-Pizzini Source Type: research
Transfusion in Neonatal Patients
Transfusion of red blood cells, platelets, and fresh frozen plasma in neonatal patients has not been well characterized in the literature, with guidelines varying greatly between institutions. However, anemia and thrombocytopenia are highly prevalent, especially in preterm neonates. When transfusing a neonatal patient, clinicians must take into consideration physiologic differences, gestational and postnatal age, congenital disorders, and maternal factors while weighing the risks and benefits of transfusion. This review of existing literature summarizes current evidence-based neonatal transfusion guidelines and highlights ...
Source: Clinics in Laboratory Medicine - December 22, 2020 Category: Laboratory Medicine Authors: Patricia E. Zerra, Cassandra D. Josephson Source Type: research
Pediatric Hemovigilance and Adverse Transfusion Reactions
Some types of transfusion reactions occur more frequently in the pediatric than the adult population. Allergic reactions are the most common, followed by nonhemolytic transfusion reactions; male children seem most susceptible to such reactions. Platelets are often implicated and pulmonary reactions are understudied in children. Clinical sequelae in neonates, such as bronchopulmonary dysplasia/chronic lung disease and intraventricular hemorrhage, have received increasing attention in relation to transfusion. There is a need to better understand the pathophysiology of transfusion reactions in neonatal and pediatric populatio...
Source: Clinics in Laboratory Medicine - December 22, 2020 Category: Laboratory Medicine Authors: Nataliya Sostin, Jeanne E. Hendrickson Source Type: research
Transfusion and Cellular Therapy in Pediatric Sickle Cell Disease
This article discusses RBC transfusion indications and complications, transfusion support during HSCT, and HSC mobilization and collection for autologous HSCT with gene therapy. (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - December 22, 2020 Category: Laboratory Medicine Authors: Yan Zheng, Stella T. Chou Source Type: research
Hemolytic Disease of the Fetus and Newborn
Hemolytic disease of the fetus and newborn (HDFN) is an immune-mediated disorder affecting neonates globally, with a range of clinical presentations from severe and life threatening to mild or even asymptomatic. Historically, HDFN has been responsible for a large proportion of perinatal mortality, and, despite advances in diagnosis and management, this morbidity and mortality has not been eradicated. Blood banking techniques and blood transfusion have contributed to improved prophylaxis and management, drastically improving the outcome of newborns with HDFN over the last century. (Source: Clinics in Laboratory Medicine)
Source: Clinics in Laboratory Medicine - December 22, 2020 Category: Laboratory Medicine Authors: Melanie E. Jackson, Jillian M. Baker Source Type: research
