Periodontal Tissue Regeneration by Transplantation of Autologous Adipose Tissue-Derived Multi-Lineage Progenitor Cells With Carbonate Apatite
In this study, we selected carbonate apatite as a candidate scaffold material for ADMPCs and conducted an in vitro examination of its effect on the cellular function of ADMPCs. We further performed autologous ADMPC transplantation with carbonate apatite as the scaffold material in a model of one-wall bone defects in beagles and then analyzed the effect on periodontal tissue regeneration. The findings showed that carbonate apatite did not affect the cell morphology of ADMPCs and that it promoted proliferation. Moreover, no effect on secretor factor transcription was found. The results of the in vivo analysis confirmed the s...
Source: Cell Transplantation - September 15, 2023 Category: Cytology Authors: Masahide Takedachi Kohsuke Kawasaki Keigo Sawada Kazuma Sakura Mari Murata Junpei Shimomura Kazuma Kawakami Chiaki Morimoto Koji Miki Noboru Takeshita Tomoaki Iwayama Hanayuki Okura Akifumi Matsuyama Masahiro Saito Masahiro Kitamura Shinya Murakami Source Type: research
Periodontal Tissue Regeneration by Transplantation of Autologous Adipose Tissue-Derived Multi-Lineage Progenitor Cells With Carbonate Apatite
In this study, we selected carbonate apatite as a candidate scaffold material for ADMPCs and conducted an in vitro examination of its effect on the cellular function of ADMPCs. We further performed autologous ADMPC transplantation with carbonate apatite as the scaffold material in a model of one-wall bone defects in beagles and then analyzed the effect on periodontal tissue regeneration. The findings showed that carbonate apatite did not affect the cell morphology of ADMPCs and that it promoted proliferation. Moreover, no effect on secretor factor transcription was found. The results of the in vivo analysis confirmed the s...
Source: Cell Transplantation - September 15, 2023 Category: Cytology Authors: Masahide Takedachi Kohsuke Kawasaki Keigo Sawada Kazuma Sakura Mari Murata Junpei Shimomura Kazuma Kawakami Chiaki Morimoto Koji Miki Noboru Takeshita Tomoaki Iwayama Hanayuki Okura Akifumi Matsuyama Masahiro Saito Masahiro Kitamura Shinya Murakami Source Type: research
Periodontal Tissue Regeneration by Transplantation of Autologous Adipose Tissue-Derived Multi-Lineage Progenitor Cells With Carbonate Apatite
In this study, we selected carbonate apatite as a candidate scaffold material for ADMPCs and conducted an in vitro examination of its effect on the cellular function of ADMPCs. We further performed autologous ADMPC transplantation with carbonate apatite as the scaffold material in a model of one-wall bone defects in beagles and then analyzed the effect on periodontal tissue regeneration. The findings showed that carbonate apatite did not affect the cell morphology of ADMPCs and that it promoted proliferation. Moreover, no effect on secretor factor transcription was found. The results of the in vivo analysis confirmed the s...
Source: Cell Transplantation - September 15, 2023 Category: Cytology Authors: Masahide Takedachi Kohsuke Kawasaki Keigo Sawada Kazuma Sakura Mari Murata Junpei Shimomura Kazuma Kawakami Chiaki Morimoto Koji Miki Noboru Takeshita Tomoaki Iwayama Hanayuki Okura Akifumi Matsuyama Masahiro Saito Masahiro Kitamura Shinya Murakami Source Type: research
Role of Serum Inflammatory Cytokines in Sepsis Rats Following BMSCs Transplantation: Protein Microarray Analysis
In this study, we investigated the effects of BMSCs on serum inflammatory cytokines in a rat model of lipopolysaccharide (LPS)-induced sepsis. Sepsis was induced by intravenous injection of LPS, followed by transplantation of BMSCs. We monitored survival rates for 72 h and evaluated organ functions, histopathological changes, and cytokines expression. Sepsis rats showed decreased levels of white blood cells, platelets, lymphocyte ratio, and oxygen partial pressure, along with increased levels of neutrophil ratio, carbon dioxide partial pressure, lactic acid, alanine aminotransferase, and aspartate aminotransferase. Histolo...
Source: Cell Transplantation - September 14, 2023 Category: Cytology Authors: Guanghui Xiu Xiuling Li Juan Chen Jintao Li Kun Chen Ping Liu Bin Ling Ying Yang Source Type: research
Haploidentical Natural Killer Cell Therapy as an Adjunct to Stem Cell Transplantation for Treatment of Refractory Acute Myeloid Leukemia
Cell Transplant. 2023 Jan-Dec;32:9636897231198178. doi: 10.1177/09636897231198178.ABSTRACTRefractory acute myeloid leukemia (AML), defined as failure of two cycles of induction therapy at diagnosis or of one cycle at relapse, represents a subgroup with poor outcomes. Haploidentical natural killer cell (NK) therapy is a strategy that is being explored in refractory malignancies. Historically, at our center, patients with refractory AML have been treated with cytoreductive therapy (fludarabine + cytosine + granulocyte colony-stimulating factor ± idarubicin or mitoxantrone + etoposide) followed by 1-week rest and then reduce...
Source: Cell Transplantation - September 14, 2023 Category: Cytology Authors: Uday Kulkarni Arun Kumar Arunachalam Hamenth Kumar Palani Reeshma Radhakrishnan Nair Nithya Balasundaram Arvind Venkatraman Anu Korula Sushil Selvarajan Sharon Lionel Poonkuzhali Balasubramanian Madhavi Maddali Aby Abraham Biju George Vikram Mathews Source Type: research
Repairing Mechanisms for Distal Airway Injuries and Related Targeted Therapeutics for Chronic Lung Diseases
Cell Transplant. 2023 Jan-Dec;32:9636897231196489. doi: 10.1177/09636897231196489.ABSTRACTChronic lung diseases, such as chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis (IPF), involve progressive and irreversible destruction and pathogenic remodeling of airways and have become the leading health care burden worldwide. Pulmonary tissue has extensive capacities to launch injury-responsive repairing programs (IRRPs) to replace the damaged or dead cells upon acute lung injuries. However, the IRRPs are frequently compromised in chronic lung diseases. In this review, we aim to provide an overview o...
Source: Cell Transplantation - September 12, 2023 Category: Cytology Authors: Huahua Xu Guihong Pan Jun Wang Source Type: research
Coating-Free Culture Medium for Establishing and Maintaining Human Induced Pluripotent Stem Cells
In this study, we develop an innovative coating-free medium for expanding hPSCs. The xenofree medium supports the weekend-free culture and competitive growth of hPSCs on several cell culture plastics without an additional pre-coating process. The pluripotent stemness of the expanded cells is stably sustained for more than 10 passages, featured with high pluripotent marker expressions, normal karyotyping, and differentiating capacity for three germ layers. The expression levels of some integrins are reduced, compared with those of the hPSCs on Matrigel. This medium also successfully supports the clonal expansion and induced...
Source: Cell Transplantation - September 12, 2023 Category: Cytology Authors: Chih-Yao Lin Yu-Yun Ching Shih-Fang Wu Yi-Ko Lee Hueng-Chuen Fan Liang-Yu Su Su-Yi Tsai Yu-Ching Chen Ching-I Shen Hong-Lin Su Source Type: research
Repairing Mechanisms for Distal Airway Injuries and Related Targeted Therapeutics for Chronic Lung Diseases
Cell Transplant. 2023 Jan-Dec;32:9636897231196489. doi: 10.1177/09636897231196489.ABSTRACTChronic lung diseases, such as chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis (IPF), involve progressive and irreversible destruction and pathogenic remodeling of airways and have become the leading health care burden worldwide. Pulmonary tissue has extensive capacities to launch injury-responsive repairing programs (IRRPs) to replace the damaged or dead cells upon acute lung injuries. However, the IRRPs are frequently compromised in chronic lung diseases. In this review, we aim to provide an overview o...
Source: Cell Transplantation - September 12, 2023 Category: Cytology Authors: Huahua Xu Guihong Pan Jun Wang Source Type: research
Coating-Free Culture Medium for Establishing and Maintaining Human Induced Pluripotent Stem Cells
In this study, we develop an innovative coating-free medium for expanding hPSCs. The xenofree medium supports the weekend-free culture and competitive growth of hPSCs on several cell culture plastics without an additional pre-coating process. The pluripotent stemness of the expanded cells is stably sustained for more than 10 passages, featured with high pluripotent marker expressions, normal karyotyping, and differentiating capacity for three germ layers. The expression levels of some integrins are reduced, compared with those of the hPSCs on Matrigel. This medium also successfully supports the clonal expansion and induced...
Source: Cell Transplantation - September 12, 2023 Category: Cytology Authors: Chih-Yao Lin Yu-Yun Ching Shih-Fang Wu Yi-Ko Lee Hueng-Chuen Fan Liang-Yu Su Su-Yi Tsai Yu-Ching Chen Ching-I Shen Hong-Lin Su Source Type: research
Repairing Mechanisms for Distal Airway Injuries and Related Targeted Therapeutics for Chronic Lung Diseases
Cell Transplant. 2023 Jan-Dec;32:9636897231196489. doi: 10.1177/09636897231196489.ABSTRACTChronic lung diseases, such as chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis (IPF), involve progressive and irreversible destruction and pathogenic remodeling of airways and have become the leading health care burden worldwide. Pulmonary tissue has extensive capacities to launch injury-responsive repairing programs (IRRPs) to replace the damaged or dead cells upon acute lung injuries. However, the IRRPs are frequently compromised in chronic lung diseases. In this review, we aim to provide an overview o...
Source: Cell Transplantation - September 12, 2023 Category: Cytology Authors: Huahua Xu Guihong Pan Jun Wang Source Type: research
Coating-Free Culture Medium for Establishing and Maintaining Human Induced Pluripotent Stem Cells
In this study, we develop an innovative coating-free medium for expanding hPSCs. The xenofree medium supports the weekend-free culture and competitive growth of hPSCs on several cell culture plastics without an additional pre-coating process. The pluripotent stemness of the expanded cells is stably sustained for more than 10 passages, featured with high pluripotent marker expressions, normal karyotyping, and differentiating capacity for three germ layers. The expression levels of some integrins are reduced, compared with those of the hPSCs on Matrigel. This medium also successfully supports the clonal expansion and induced...
Source: Cell Transplantation - September 12, 2023 Category: Cytology Authors: Chih-Yao Lin Yu-Yun Ching Shih-Fang Wu Yi-Ko Lee Hueng-Chuen Fan Liang-Yu Su Su-Yi Tsai Yu-Ching Chen Ching-I Shen Hong-Lin Su Source Type: research
Injectable Decorin/Gellan Gum Hydrogel Encapsulating Adipose-Derived Stem Cells Enhances Anti-Inflammatory Effect in Cartilage Injury via Autophagy Signaling
Cell Transplant. 2023 Jan-Dec;32:9636897231196493. doi: 10.1177/09636897231196493.ABSTRACTAdipose-derived stem cells (ADSCs) are employed as a promising alternative in treating cartilage injury. Regulating the inflammatory "fingerprint" of ADSCs to improve their anti-inflammatory properties could enhance therapy efficiency. Herein, a novel injectable decorin/gellan gum hydrogel combined with ADSCs encapsulation for arthritis cartilage treatment is proposed. Decorin/gellan gum hydrogel was prepared according to the previous manufacturing protocol. The liquid-solid form transition of gellan gum hydrogel is perfectly suitable...
Source: Cell Transplantation - September 9, 2023 Category: Cytology Authors: Weiping He Yu Wu Zhihong Luo Genghua Yang Woquan Ye Xi Chen Jianhua Ren Tangzhao Liang Zhiqiang Liao Shihai Jiang Kun Wang Source Type: research
Injectable Decorin/Gellan Gum Hydrogel Encapsulating Adipose-Derived Stem Cells Enhances Anti-Inflammatory Effect in Cartilage Injury via Autophagy Signaling
Cell Transplant. 2023 Jan-Dec;32:9636897231196493. doi: 10.1177/09636897231196493.ABSTRACTAdipose-derived stem cells (ADSCs) are employed as a promising alternative in treating cartilage injury. Regulating the inflammatory "fingerprint" of ADSCs to improve their anti-inflammatory properties could enhance therapy efficiency. Herein, a novel injectable decorin/gellan gum hydrogel combined with ADSCs encapsulation for arthritis cartilage treatment is proposed. Decorin/gellan gum hydrogel was prepared according to the previous manufacturing protocol. The liquid-solid form transition of gellan gum hydrogel is perfectly suitable...
Source: Cell Transplantation - September 9, 2023 Category: Cytology Authors: Weiping He Yu Wu Zhihong Luo Genghua Yang Woquan Ye Xi Chen Jianhua Ren Tangzhao Liang Zhiqiang Liao Shihai Jiang Kun Wang Source Type: research
Injectable Decorin/Gellan Gum Hydrogel Encapsulating Adipose-Derived Stem Cells Enhances Anti-Inflammatory Effect in Cartilage Injury via Autophagy Signaling
Cell Transplant. 2023 Jan-Dec;32:9636897231196493. doi: 10.1177/09636897231196493.ABSTRACTAdipose-derived stem cells (ADSCs) are employed as a promising alternative in treating cartilage injury. Regulating the inflammatory "fingerprint" of ADSCs to improve their anti-inflammatory properties could enhance therapy efficiency. Herein, a novel injectable decorin/gellan gum hydrogel combined with ADSCs encapsulation for arthritis cartilage treatment is proposed. Decorin/gellan gum hydrogel was prepared according to the previous manufacturing protocol. The liquid-solid form transition of gellan gum hydrogel is perfectly suitable...
Source: Cell Transplantation - September 9, 2023 Category: Cytology Authors: Weiping He Yu Wu Zhihong Luo Genghua Yang Woquan Ye Xi Chen Jianhua Ren Tangzhao Liang Zhiqiang Liao Shihai Jiang Kun Wang Source Type: research
HLA Fully-Mismatched Sibling-Derived CD7 CAR-T Therapy Bridging to Haploidentical Hematopoietic Stem Cell Transplantation for Hepatosplenic γδ T-cell Lymphoma
Cell Transplant. 2023 Jan-Dec;32:9636897231194265. doi: 10.1177/09636897231194265.ABSTRACTWhile chimeric antigen receptor (CAR)-T-cell therapy has demonstrated remarkable effectiveness in the treatment of B-cell lymphomas and leukemias, research on T-cell malignancies is still limited. Here, we reported a patient with hepatosplenic γδ T-cell lymphoma refractory to multiple lines of chemotherapy, who eventually achieved first complete remission with flow cytometry-confirmed minimal residual disease negativity after human leukocyte antigen (HLA) fully-mismatched sibling-derived CD7 CAR-T therapy. However, given the allogen...
Source: Cell Transplantation - September 5, 2023 Category: Cytology Authors: Xueer Xu Cheng Zu Mingming Zhang Pingnan Xiao Ruimin Hong Jingjing Feng Huijun Xu Jiazhen Cui Jian Yu Jimin Shi Guoqing Wei Alex H Chang He Huang Yongxian Hu Source Type: research
