Investigation of Allogeneic Neutrophil Transfusion in Improving Survival Rates of Severe Infection Mice
Cell Transplant. 2024 Jan-Dec;33:9636897241228031. doi: 10.1177/09636897241228031.ABSTRACTThe management of granulocytopenia-associated infections is challenging, and a high mortality rate is associated with traditional supportive therapies. Neutrophils-the primary defenders of the human immune system-have potent bactericidal capabilities. Here, we investigated the dynamic in vivo distribution of neutrophil transfusion and their impact on the treatment outcome of severe granulocytopenic infections. We transfused 89Zr-labeled neutrophils in the C57BL/6 mice and observed the dynamic neutrophil distribution in mice for 24 h u...
Source: Cell Transplantation - February 14, 2024 Category: Cytology Authors: Linbin Li Yunxi Yang Zaiwen Guo Xi Gao Lu Liu Jiamin Huang Bingwei Sun Source Type: research

Human Placenta-Derived Mesenchymal Stem Cells Combined With Artificial Dermal Scaffold Enhance Wound Healing in a Tendon-Exposed Wound of a Rabbit Model
Cell Transplant. 2024 Jan-Dec;33:9636897241228922. doi: 10.1177/09636897241228922.ABSTRACTTo overcome the difficulty of vascular regeneration in exposed tendon wounds, we combined human placenta-derived mesenchymal stem cells (hPMSCs) with an artificial dermal scaffold and assessed their role in promoting vascular regeneration and wound healing in vivo. hPMSCs were isolated from the human placenta and characterized based on their morphology, phenotypic profiles, and pluripotency. New Zealand rabbits were used to establish an exposed tendon wound model, and hPMSCs and artificial dermal scaffolds were transplanted into the w...
Source: Cell Transplantation - February 9, 2024 Category: Cytology Authors: Fang Li Cong Gao Guodong Song Kun Zhang Guobao Huang Hua Liu Source Type: research

Xenogenic Application of Human Placenta-Derived Mesenchymal Stromal Cells in a Porcine Large Animal Model
Cell Transplant. 2024 Jan-Dec;33:9636897241226737. doi: 10.1177/09636897241226737.ABSTRACTIn animal models, cell therapies for different diseases or injuries have been very successful. Preclinical studies with cells aiming at a stroke, heart attack, and other emergency situations were promising but sometimes failed translation in clinical situations. We, therefore, investigated if human placenta-derived mesenchymal stromal cells can be injected in pigs without provoking rejection to serve as a xenogenic transplantation model to bridge preclinical animal studies to more promising future preclinical studies. Male human place...
Source: Cell Transplantation - February 7, 2024 Category: Cytology Authors: Niklas Harland Jasmin Knoll Bastian Amend Tanja Abruzzese Harald Abele Peter Jakubowski Arnulf Stenzl Wilhelm K Aicher Source Type: research

RNA-Seq Analysis Reveals Potential Neuroprotective Mechanisms of Pachymic Acid Toward Iron-Induced Oxidative Stress and Cell Death
In this study, we test whether PA have neuroprotection effect aganist ferrous ions induced toxicity in SH-SY5Y cells. It was found that pre-treatment with PA reduced intracellular ROS levels, increased mitochondrial membrane potential, and protected cells from apoptotic death. RNA-seq and qRT-PCR results indicated that PA can regulate the key genes IL1B, CXCL8, CCL7, and LRP1 on the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, such as NF-κB signaling pathway, IL-17 signaling pathway, to prevent Fe2+-induced apoptotic cell death. Our research indicated that PA has potential therapeutic effects on the neuroprote...
Source: Cell Transplantation - February 5, 2024 Category: Cytology Authors: Shuyang Hu Baili Yang Binbin Li Qianqian Fan Tinglong Wu Shanshan Li Dong Wang Tao Yang Zhenghua Song Source Type: research

Sulfated Chitosan Nanofibrous Scaffolds Seeded With Adipose Stem Cells Promote Ischemic Wound Healing in a Proangiogenic Strategy
In this study, a nano-scaffold system, constructed from ADSCs seeded on electrospun fibers of modified PLGA, was designed to promote ischemic wound healing. The S-PLGA nanofiber membrane loaded with adipose stem cells ADSCs@S-PLGA was prepared by a co-culture in vitro, and the adhesion and compatibility of cells on the nano-scaffolds were explored. Scanning electron microscopy was used to observe the growth state and morphological changes of ADSCs after co-culture with PLGA electrospun fibers. The proliferation and apoptosis after co-culture were detected using a Cell Counting Kit-8 kit and flow cytometry, respectively. An...
Source: Cell Transplantation - January 30, 2024 Category: Cytology Authors: Xi Zhang Yan Jiao Tong Shen Yuanman Yu Zhou Yu Juanli Dang Lin Chen Yu Zhang Guofang Shen Source Type: research

Outcomes of Allogeneic Hematopoietic Stem Cell Transplantation in Adult Patients With Acute Myeloid Leukemia Harboring < em > KMT2A < /em > Rearrangement and Its Prognostic Factors
Cell Transplant. 2024 Jan-Dec;33:9636897231225821. doi: 10.1177/09636897231225821.ABSTRACTKMT2A rearrangement (KMT2A-r) in patients with acute myeloid leukemia (AML) is associated with poor outcomes; the prognostic factors after allogeneic hematopoietic stem cell transplantation (allo-HSCT) remain unclear. We investigated 364 adults with AML who underwent allo-HSCT between April 2016 and May 2022, and 45 had KMT2A-r among them. Propensity score analysis with 1:1 matching and the nearest neighbor matching method identified 42 patients in KMT2A-r and non-KMT2A-r cohorts, respectively. The 2-year overall survival (OS), relaps...
Source: Cell Transplantation - January 25, 2024 Category: Cytology Authors: Bingqian Jiang Yanmin Zhao Yi Luo Jian Yu Yi Chen Baodong Ye Huarui Fu Xiaoyu Lai Lizhen Liu Yishan Ye Weiyan Zheng Jie Sun Jingsong He Yi Zhao Guoqing Wei Zhen Cai He Huang Jimin Shi Source Type: research

Effect of High BMI on Human Bone Marrow-Derived Mesenchymal Stromal Cells
In conclusion, high BMI suppressed the clonogenicity of female and male hBMSCs at P4, improved the in vitro osteogenesis of female hBMSCs, and decreased the expressions of CD200 on hBMSCs in females and SSEA4 in males.PMID:38258516 | PMC:PMC10807335 | DOI:10.1177/09636897241226546 (Source: Cell Transplantation)
Source: Cell Transplantation - January 23, 2024 Category: Cytology Authors: Qiang Zong Katrin Bundkirchen Claudia Neunaber Sandra Noack Source Type: research

Mitigating Cross-Species Viral Infections in Xenotransplantation: Progress, Strategies, and Clinical Outlook
Cell Transplant. 2024 Jan-Dec;33:9636897241226849. doi: 10.1177/09636897241226849.ABSTRACTXenotransplantation holds great promise as a solution to address the critical shortage of organs, but it raises concerns regarding the potential transmission of porcine viruses to recipients, leading to infections and even zoonotic diseases. Data used in this review were mainly from literature of Pubmed database. Keywords included xenotransplantation, infection, virus, and epidemiology. The original articles and critical reviews selected were relevant to this review's theme. We review the major viral infections of concern in xenotrans...
Source: Cell Transplantation - January 23, 2024 Category: Cytology Authors: Yenong Zhou Shuyu Zhou Qian Wang Bing Zhang Source Type: research

< em > Withania Somnifera < /em > Extract Mitigates Experimental Acute Graft versus Host Disease Without Abrogating Graft Versus Leukemia Effect
Cell Transplant. 2024 Jan-Dec;33:9636897241226573. doi: 10.1177/09636897241226573.ABSTRACTAcute graft versus host disease (aGvHD) is the major contributor of nonrelapse mortality in alloHSCT. It is associated with an inflammatory immune response manifesting as cytokine storm with ensuing damage to target organs such as liver, gut, and skin. Prevention of aGvHD while retaining the beneficial graft versus leukemia (GvL) effect remains a major challenge. Withania somnifera extract (WSE) is known for its anti-inflammatory, immune-modulatory, and anticancer properties, which are appealing in the context of aGvHD. Herein, we dem...
Source: Cell Transplantation - January 23, 2024 Category: Cytology Authors: Saurabh Kumar Gupta Dievya Gohil Mohd Bashar Momin Subhash Yadav Akanksha Chichra Sachin Punatar Anant Gokarn Sumeet Mirgh Nishant Jindal Lingaraj Nayak Lal Hingorani Navin Khattry Vikram Gota Source Type: research

Firefly Rats: Illuminating the Scientific Community in Transplantation Research
Cell Transplant. 2024 Jan-Dec;33:9636897231224174. doi: 10.1177/09636897231224174.ABSTRACTFireflies produce light through luciferase-catalyzed reactions involving luciferin, oxygen, and adenosine triphosphate, distinct from other luminescent organisms. This unique feature has revolutionized molecular biology and physiology, serving as a valuable tool for cellular research. Luciferase-based bioluminescent imaging enabled the creation of transgenic animals, such as Firefly Rats. Firefly Rats, created in 2006, ubiquitously express luciferase and have become a critical asset in scientific investigations. These rats have signif...
Source: Cell Transplantation - January 18, 2024 Category: Cytology Authors: Eiji Kobayashi Yoji Hakamata Shin Enosawa Kuang-Ming Shang Hirotake Komatsu Source Type: research

Stem Cells and Their Derivatives: Unlocking the Promising Potential of Minimally Manipulated Cells for < em > In Situ < /em > Tissue Engineering
Cell Transplant. 2024 Jan-Dec;33:9636897231221846. doi: 10.1177/09636897231221846.ABSTRACTWe've read with great interest the article by Smolinska et al. entitled "Stem Cells and Their Derivatives: An Implication for the Regeneration of Nonunion Fractures" regarding the recent scientific studies dealing with the treatment of nonunion fractures in clinical and preclinical settings using Mesenchymal Stem Cell (MSC)-based therapeutic techniques. Bone tissue regeneration is a dynamic process that involves the restoration of damaged or lost bone structure and function. Traditional approaches such as autografts and allografts, pl...
Source: Cell Transplantation - January 18, 2024 Category: Cytology Authors: Ilya Klabukov Denis Baranovskii Source Type: research

A Comparison Between < em > GalT < /em > < sup > -/- < /sup > ; < em > hCD39;hCD55 < /em > and < em > GalT < /em > < sup > -/- < /sup > ; < em > hCD39;hCD46;hCD55;TBM < /em > Pig Kidneys Transplanted in Nonhuman Primates
In this study, we utilized GalT-/-;hCD39;hCD55 and GalT-/-;hCD39;hCD46;hCD55;thrombomodulin (TBM) pigs for transplantation in nonhuman primates (NHPs). The NHPs survived for 4 weeks after kidney transplantation (4 WAT) from the GalT-/-;hCD39;hCD55 pig and for 6 WAT from the GalT-/-;hCD39;hCD46;hCD55;TBM pig. However, messenger RNA (mRNA) sequencing and immunohistochemistry analysis revealed that the 6 WAT kidney exhibited more severe apoptosis, inflammation, loss of renal function, and renal fibrosis than the 4 WAT kidney. These results indicate that additional knockin of complement regulator (hCD46) and coagulation regula...
Source: Cell Transplantation - January 17, 2024 Category: Cytology Authors: Han Na Suh Ju Young Lee Hee Jung Kang Eun Mi Park Ik Jin Yun Wan Seop Kim Kimyung Choi Jeong Ho Hwang Source Type: research

Safety and Tolerability of Intra-Articular Injection of Adipose-Derived Mesenchymal Stem Cells GXCPC1 in 11 Subjects With Knee Osteoarthritis: A Nonrandomized Pilot Study Without a Control Arm
Cell Transplant. 2024 Jan-Dec;33:9636897231221882. doi: 10.1177/09636897231221882.ABSTRACTThe current study aimed to determine the safety profile of intra-articular-injected allogeneic adipose-derived mesenchymal stem cells (ADSCs) GXCPC1 in subjects with knee osteoarthritis (OA) and its preliminary efficacy outcome. The 3 + 3 phase I study was designed with two dose-escalation cohorts: low dose (6.7 × 106 GXCPC1, N = 5) and high dose (4 × 107 GXCPC1, N = 6). The primary endpoint was safety, which was evaluated by recording adverse events throughout the trial; the secondary endpoints included total, pain, stiffness, and ...
Source: Cell Transplantation - January 11, 2024 Category: Cytology Authors: Cheng-Fong Chen Yi-Chung Chen Yu-Show Fu Shang-Wen Tsai Po-Kuei Wu Chao-Ming Chen Wei-Ming Chen Hung-Ta Hondar Wu Chia-Hsin Lee Chao-Liang Chang Po-Cheng Lin Yong-Cheng Kao Chun-Hung Chen Ming-Hsi Chuang Source Type: research

Ultrasound-Targeted Microbubble Destruction Increases BBB Permeability and Promotes Stem Cell-Induced Regeneration of Stroke by Downregulating MMP8
The objective of this study was to evaluate the feasibility, safety, and effectiveness of intravenous stem cell delivery utilizing ultrasound-targeted microbubble destruction (UTMD) in a rat model of middle cerebral artery occlusion (MCAO), while investigating the underlying mechanisms. Acute cerebral infarction (ACI) was induced surgically in adult rats to create the MCAO rat model. Intravenous injection of SonoVue microbubbles and bone marrow-derived mesenchymal stem cells (BMSC) was performed concurrently, with or without ultrasound targeting the stroke. The animals were divided into four groups: sham-operated group, AC...
Source: Cell Transplantation - January 9, 2024 Category: Cytology Authors: Yun Bai Yichao Du Yin Yang Thomas W älchli Paul E Constanthin Fan Li Source Type: research

Ultrasound-Targeted Microbubble Destruction Increases BBB Permeability and Promotes Stem Cell-Induced Regeneration of Stroke by Downregulating MMP8
The objective of this study was to evaluate the feasibility, safety, and effectiveness of intravenous stem cell delivery utilizing ultrasound-targeted microbubble destruction (UTMD) in a rat model of middle cerebral artery occlusion (MCAO), while investigating the underlying mechanisms. Acute cerebral infarction (ACI) was induced surgically in adult rats to create the MCAO rat model. Intravenous injection of SonoVue microbubbles and bone marrow-derived mesenchymal stem cells (BMSC) was performed concurrently, with or without ultrasound targeting the stroke. The animals were divided into four groups: sham-operated group, AC...
Source: Cell Transplantation - January 9, 2024 Category: Cytology Authors: Yun Bai Yichao Du Yin Yang Thomas W älchli Paul E Constanthin Fan Li Source Type: research