Ultrasound-Targeted Microbubble Destruction Increases BBB Permeability and Promotes Stem Cell-Induced Regeneration of Stroke by Downregulating MMP8
The objective of this study was to evaluate the feasibility, safety, and effectiveness of intravenous stem cell delivery utilizing ultrasound-targeted microbubble destruction (UTMD) in a rat model of middle cerebral artery occlusion (MCAO), while investigating the underlying mechanisms. Acute cerebral infarction (ACI) was induced surgically in adult rats to create the MCAO rat model. Intravenous injection of SonoVue microbubbles and bone marrow-derived mesenchymal stem cells (BMSC) was performed concurrently, with or without ultrasound targeting the stroke. The animals were divided into four groups: sham-operated group, AC...
Source: Cell Transplantation - January 9, 2024 Category: Cytology Authors: Yun Bai Yichao Du Yin Yang Thomas W älchli Paul E Constanthin Fan Li Source Type: research
Relapsed/Refractory Peripheral T-Cell Lymphoma-Associated Hemophagocytic Lymphohistiocytosis With UNC13D and CD27 Germline Mutations
This study underscores the efficacy of genetic tests in identifying significant mutations and confirming their etiologies, providing an early basis for treatment decisions and the selection of suitable transplant donors.PMID:38183241 | PMC:PMC10771736 | DOI:10.1177/09636897231221887 (Source: Cell Transplantation)
Source: Cell Transplantation - January 6, 2024 Category: Cytology Authors: Tingting Yang Rongrong Chen Mingming Zhang Ruirui Jing Jia Geng Guoqing Wei Yi Luo Pingnan Xiao Ruimin Hong Jingjing Feng Shan Fu Houli Zhao Jiazhen Cui Simao Huang He Huang Yongxian Hu Source Type: research
Relapsed/Refractory Peripheral T-Cell Lymphoma-Associated Hemophagocytic Lymphohistiocytosis With UNC13D and CD27 Germline Mutations
This study underscores the efficacy of genetic tests in identifying significant mutations and confirming their etiologies, providing an early basis for treatment decisions and the selection of suitable transplant donors.PMID:38183241 | PMC:PMC10771736 | DOI:10.1177/09636897231221887 (Source: Cell Transplantation)
Source: Cell Transplantation - January 6, 2024 Category: Cytology Authors: Tingting Yang Rongrong Chen Mingming Zhang Ruirui Jing Jia Geng Guoqing Wei Yi Luo Pingnan Xiao Ruimin Hong Jingjing Feng Shan Fu Houli Zhao Jiazhen Cui Simao Huang He Huang Yongxian Hu Source Type: research
Relapsed/Refractory Peripheral T-Cell Lymphoma-Associated Hemophagocytic Lymphohistiocytosis With UNC13D and CD27 Germline Mutations
This study underscores the efficacy of genetic tests in identifying significant mutations and confirming their etiologies, providing an early basis for treatment decisions and the selection of suitable transplant donors.PMID:38183241 | DOI:10.1177/09636897231221887 (Source: Cell Transplantation)
Source: Cell Transplantation - January 6, 2024 Category: Cytology Authors: Tingting Yang Rongrong Chen Mingming Zhang Ruirui Jing Jia Geng Guoqing Wei Yi Luo Pingnan Xiao Ruimin Hong Jingjing Feng Shan Fu Houli Zhao Jiazhen Cui Simao Huang He Huang Yongxian Hu Source Type: research
Preclinical Animal Study and Pilot Clinical Trial of Using Enriched Peripheral Blood-Derived Mononuclear Cells for Intervertebral Disc Degeneration
This study investigates the effect of human peripheral blood-derived mononuclear cells (PBMCs) on pain relief and life quality improvement in IVDD patients. The enriched monocytes of the PBMCs could differentiate into CD14 and CD206 double-positive M2 macrophages in vitro. Preclinical evidence in rats showed that the transplanted PBMCs exhibited anti-inflammatory and moderate tissue-repair effects on controlling IVDD progress in the rat model. The PBMCs significantly steered the aggrecan and type II collagen expressions and attenuated the pro-inflammatory cytokines in the affected disk. Based on the animal results, 36 pati...
Source: Cell Transplantation - January 4, 2024 Category: Cytology Authors: Yu-Hsuan Chung Ming-Hsien Hu Shang-Chyi Kao Ying-Hsien Kao Fu-Hui Wang Chia-Ying Hsieh Ching-I Shen Chang-Han Chuang Dave Wei-Chih Chen Chi-Chung Kuo Hong-Lin Su Chih-Lung Lin Source Type: research
Multifaceted Characterization of Human Embryonic Stem Cell-Derived Mesenchymal Stem/Stromal Cells Revealed Amelioration of Acute Liver Injury in NOD-SCID Mice
In this study, we took advantage of the well-established twist-related protein 1 (TWIST1)-overexpressing hESCs and two small molecular cocktails (CHIR99021, decitabine) for high-efficient MSC induction. To assess the multidimensional biological and transcriptomic characteristics, we turned to cellular and molecular methods, such as flow cytometry (FCM), quantitative reverse transcription-polymerase chain reaction (qRT-PCR), in vitro tri-lineage differentiation, cytokine secretion analysis, in vivo transplantation for acute liver injury (ALI) management, and bioinformatics analyses (eg, gene ontology-biological processes [G...
Source: Cell Transplantation - January 4, 2024 Category: Cytology Authors: Youlai Zhang Ying He Rufei Deng Zhenyu Jiang Leisheng Zhang Yuanlin Zeng Lijin Zou Source Type: research
Repairing Effect of Mesenchymal Stem Cells on Lead Acetate-Induced Testicular Injury in Mice
In this study, we assessed the repairing effects of human umbilical cord mesenchymal stem cells (MSCs) on testicular injury caused by lead acetate in mice. MSCs were injected into mice with testicular injury by intraperitoneal injection, and the organ coefficient of reproductive organs, sperm motility, hormone level and antioxidant index of mice were tested. Compared with the normal group, the coefficient of reproductive organs and sperm motility were reduced in the model group, and histopathology showed obvious testicular injury, proving successful modeling. Compared with the model group, the reproductive organ coefficien...
Source: Cell Transplantation - January 4, 2024 Category: Cytology Authors: Shasha Zhao Zhaozhi Li Kun Li Xiaoyu Dai Zhe Xu Li Li Huanhuan Wang Xiaodun Liu Dong Li Source Type: research
Preclinical Animal Study and Pilot Clinical Trial of Using Enriched Peripheral Blood-Derived Mononuclear Cells for Intervertebral Disc Degeneration
This study investigates the effect of human peripheral blood-derived mononuclear cells (PBMCs) on pain relief and life quality improvement in IVDD patients. The enriched monocytes of the PBMCs could differentiate into CD14 and CD206 double-positive M2 macrophages in vitro. Preclinical evidence in rats showed that the transplanted PBMCs exhibited anti-inflammatory and moderate tissue-repair effects on controlling IVDD progress in the rat model. The PBMCs significantly steered the aggrecan and type II collagen expressions and attenuated the pro-inflammatory cytokines in the affected disk. Based on the animal results, 36 pati...
Source: Cell Transplantation - January 4, 2024 Category: Cytology Authors: Yu-Hsuan Chung Ming-Hsien Hu Shang-Chyi Kao Ying-Hsien Kao Fu-Hui Wang Chia-Ying Hsieh Ching-I Shen Chang-Han Chuang Dave Wei-Chih Chen Chi-Chung Kuo Hong-Lin Su Chih-Lung Lin Source Type: research
Multifaceted Characterization of Human Embryonic Stem Cell-Derived Mesenchymal Stem/Stromal Cells Revealed Amelioration of Acute Liver Injury in NOD-SCID Mice
In this study, we took advantage of the well-established twist-related protein 1 (TWIST1)-overexpressing hESCs and two small molecular cocktails (CHIR99021, decitabine) for high-efficient MSC induction. To assess the multidimensional biological and transcriptomic characteristics, we turned to cellular and molecular methods, such as flow cytometry (FCM), quantitative reverse transcription-polymerase chain reaction (qRT-PCR), in vitro tri-lineage differentiation, cytokine secretion analysis, in vivo transplantation for acute liver injury (ALI) management, and bioinformatics analyses (eg, gene ontology-biological processes [G...
Source: Cell Transplantation - January 4, 2024 Category: Cytology Authors: Youlai Zhang Ying He Rufei Deng Zhenyu Jiang Leisheng Zhang Yuanlin Zeng Lijin Zou Source Type: research
Repairing Effect of Mesenchymal Stem Cells on Lead Acetate-Induced Testicular Injury in Mice
In this study, we assessed the repairing effects of human umbilical cord mesenchymal stem cells (MSCs) on testicular injury caused by lead acetate in mice. MSCs were injected into mice with testicular injury by intraperitoneal injection, and the organ coefficient of reproductive organs, sperm motility, hormone level and antioxidant index of mice were tested. Compared with the normal group, the coefficient of reproductive organs and sperm motility were reduced in the model group, and histopathology showed obvious testicular injury, proving successful modeling. Compared with the model group, the reproductive organ coefficien...
Source: Cell Transplantation - January 4, 2024 Category: Cytology Authors: Shasha Zhao Zhaozhi Li Kun Li Xiaoyu Dai Zhe Xu Li Li Huanhuan Wang Xiaodun Liu Dong Li Source Type: research
Preclinical Animal Study and Pilot Clinical Trial of Using Enriched Peripheral Blood-Derived Mononuclear Cells for Intervertebral Disc Degeneration
This study investigates the effect of human peripheral blood-derived mononuclear cells (PBMCs) on pain relief and life quality improvement in IVDD patients. The enriched monocytes of the PBMCs could differentiate into CD14 and CD206 double-positive M2 macrophages in vitro. Preclinical evidence in rats showed that the transplanted PBMCs exhibited anti-inflammatory and moderate tissue-repair effects on controlling IVDD progress in the rat model. The PBMCs significantly steered the aggrecan and type II collagen expressions and attenuated the pro-inflammatory cytokines in the affected disk. Based on the animal results, 36 pati...
Source: Cell Transplantation - January 4, 2024 Category: Cytology Authors: Yu-Hsuan Chung Ming-Hsien Hu Shang-Chyi Kao Ying-Hsien Kao Fu-Hui Wang Chia-Ying Hsieh Ching-I Shen Chang-Han Chuang Dave Wei-Chih Chen Chi-Chung Kuo Hong-Lin Su Chih-Lung Lin Source Type: research
Multifaceted Characterization of Human Embryonic Stem Cell-Derived Mesenchymal Stem/Stromal Cells Revealed Amelioration of Acute Liver Injury in NOD-SCID Mice
In this study, we took advantage of the well-established twist-related protein 1 (TWIST1)-overexpressing hESCs and two small molecular cocktails (CHIR99021, decitabine) for high-efficient MSC induction. To assess the multidimensional biological and transcriptomic characteristics, we turned to cellular and molecular methods, such as flow cytometry (FCM), quantitative reverse transcription-polymerase chain reaction (qRT-PCR), in vitro tri-lineage differentiation, cytokine secretion analysis, in vivo transplantation for acute liver injury (ALI) management, and bioinformatics analyses (eg, gene ontology-biological processes [G...
Source: Cell Transplantation - January 4, 2024 Category: Cytology Authors: Youlai Zhang Ying He Rufei Deng Zhenyu Jiang Leisheng Zhang Yuanlin Zeng Lijin Zou Source Type: research
Repairing Effect of Mesenchymal Stem Cells on Lead Acetate-Induced Testicular Injury in Mice
In this study, we assessed the repairing effects of human umbilical cord mesenchymal stem cells (MSCs) on testicular injury caused by lead acetate in mice. MSCs were injected into mice with testicular injury by intraperitoneal injection, and the organ coefficient of reproductive organs, sperm motility, hormone level and antioxidant index of mice were tested. Compared with the normal group, the coefficient of reproductive organs and sperm motility were reduced in the model group, and histopathology showed obvious testicular injury, proving successful modeling. Compared with the model group, the reproductive organ coefficien...
Source: Cell Transplantation - January 4, 2024 Category: Cytology Authors: Shasha Zhao Zhaozhi Li Kun Li Xiaoyu Dai Zhe Xu Li Li Huanhuan Wang Xiaodun Liu Dong Li Source Type: research
Proliferation and Differentiation Potential of Bone Marrow-Derived Mesenchymal Stem Cells From Children With Polydactyly and Adults With Basal Joint Arthritis
This study compared the proliferation and differentiation potential of bone marrow-derived mesenchymal stem cells (BMSCs) derived from infants with polydactyly and adults with basal joint arthritis. The proliferation rate of adult and infant BMSCs was determined by the cell number changes and doubling times. The γH2AX immunofluorescence staining, age-related gene expression, senescence-associated β-galactosidase (SA-β-gal) staining were analyzed to determine the senescence state of adult and infant BMSCs. The expression levels of superoxide dismutases (SODs) and genes associated with various types of differentiation wer...
Source: Cell Transplantation - January 2, 2024 Category: Cytology Authors: Shih-Han Yeh Jin-Huei Yu Po-Hsin Chou Szu-Hsien Wu Yu-Ting Liao Yi-Chao Huang Tung-Ming Chen Jung-Pan Wang Source Type: research
Proliferation and Differentiation Potential of Bone Marrow-Derived Mesenchymal Stem Cells From Children With Polydactyly and Adults With Basal Joint Arthritis
This study compared the proliferation and differentiation potential of bone marrow-derived mesenchymal stem cells (BMSCs) derived from infants with polydactyly and adults with basal joint arthritis. The proliferation rate of adult and infant BMSCs was determined by the cell number changes and doubling times. The γH2AX immunofluorescence staining, age-related gene expression, senescence-associated β-galactosidase (SA-β-gal) staining were analyzed to determine the senescence state of adult and infant BMSCs. The expression levels of superoxide dismutases (SODs) and genes associated with various types of differentiation wer...
Source: Cell Transplantation - January 2, 2024 Category: Cytology Authors: Shih-Han Yeh Jin-Huei Yu Po-Hsin Chou Szu-Hsien Wu Yu-Ting Liao Yi-Chao Huang Tung-Ming Chen Jung-Pan Wang Source Type: research
