Periodontal Tissue Regeneration by Transplantation of Autologous Adipose Tissue-Derived Multi-Lineage Progenitor Cells With Carbonate Apatite

In this study, we selected carbonate apatite as a candidate scaffold material for ADMPCs and conducted an in vitro examination of its effect on the cellular function of ADMPCs. We further performed autologous ADMPC transplantation with carbonate apatite as the scaffold material in a model of one-wall bone defects in beagles and then analyzed the effect on periodontal tissue regeneration. The findings showed that carbonate apatite did not affect the cell morphology of ADMPCs and that it promoted proliferation. Moreover, no effect on secretor factor transcription was found. The results of the in vivo analysis confirmed the space-making capacity of carbonate apatite, and the acquisition of significant new attachment was observed in the group involving ADMPC transplantation with carbonate apatite compared with the group involving carbonate apatite application alone. Our results demonstrate the usefulness of carbonate apatite as a scaffold material for ADMPC transplantation.PMID:37710973 | PMC:PMC10503283 | DOI:10.1177/09636897231198296

Source: Cell Transplantation - September 15, 2023 Category: Cytology Authors: Masahide Takedachi Kohsuke Kawasaki Keigo Sawada Kazuma Sakura Mari Murata Junpei Shimomura Kazuma Kawakami Chiaki Morimoto Koji Miki Noboru Takeshita Tomoaki Iwayama Hanayuki Okura Akifumi Matsuyama Masahiro Saito Masahiro Kitamura Shinya Murakami Source Type: research