Isolation, Expansion, and Endoscopic Delivery of Autologous Enteric Neuronal Stem Cells in Swine
Cell Transplant. 2023 Jan-Dec;32:9636897231215233. doi: 10.1177/09636897231215233.ABSTRACTThe enteric nervous system (ENS) is an extensive network of neurons and glia within the wall of the gastrointestinal (GI) tract that regulates many essential GI functions. Consequently, disorders of the ENS due to developmental defects, inflammation, infection, or age-associated neurodegeneration lead to serious neurointestinal diseases. Despite the prevalence and severity of these diseases, effective treatments are lacking as they fail to directly address the underlying pathology. Neuronal stem cell therapy represents a promising app...
Source: Cell Transplantation - December 4, 2023 Category: Cytology Authors: Ryo Hotta Weikang Pan Sukhada Bhave Nandor Nagy Rhian Stavely Takahiro Ohkura Kumar Krishnan Geoffrey de Couto Richard Myers Luis Rodriguez-Borlado Alan J Burns Allan M Goldstein Source Type: research
Retinogenesis in a Dish: Bibliometric Analysis and Visualization of Retinal Organoids From 2011 to 2022
Cell Transplant. 2023 Jan-Dec;32:9636897231214321. doi: 10.1177/09636897231214321.ABSTRACTRetinal organoid (RO) is the three-dimensional (3D) retinal culture derived from pluripotent or embryonic stem cells which recapitulates organ functions, which was a revolutionary milestone in stem cell technology. The purpose of this study is to explore the hotspots and future directions on ROs, as well as to better understand the fields of greatest research opportunities. Eligible publications related to RO from 2011 to 2022 were acquired from the Web of Science (WoS) Core Collection database. Bibliometric analysis was performed by ...
Source: Cell Transplantation - December 4, 2023 Category: Cytology Authors: Wenyue Shen An Shao Wuyuan Zhou Lixia Lou Andrzej Grzybowski Kai Jin Juan Ye Source Type: research
Neuroprotective Effects of Human Adipose-Derived Mesenchymal Stem Cells in Oxygen-Induced Retinopathy
This study was designed to provide evidence of the neuroprotective of human adipose-derived mesenchymal stem cells (hADSCs) in oxygen-induced retinopathy (OIR). In vivo, hADSCs were intravitreally injected into OIR mice. Various assessments, including HE (histological evaluation), TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) staining, electroretinogram (ERG) analysis, and retinal flat-mount examination, were performed separately at postnatal days 15 (P15) and 17 (P17) to evaluate neurological damage and functional changes. Western blot analysis of ciliary neurotrophic factor (CNTF), glial cell line-...
Source: Cell Transplantation - November 29, 2023 Category: Cytology Authors: Jifu Xin Lvlv Zhou Ling Zhang Kai Guo Dayong Yang Source Type: research
Neuroprotective Effects of Human Adipose-Derived Mesenchymal Stem Cells in Oxygen-Induced Retinopathy
This study was designed to provide evidence of the neuroprotective of human adipose-derived mesenchymal stem cells (hADSCs) in oxygen-induced retinopathy (OIR). In vivo, hADSCs were intravitreally injected into OIR mice. Various assessments, including HE (histological evaluation), TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) staining, electroretinogram (ERG) analysis, and retinal flat-mount examination, were performed separately at postnatal days 15 (P15) and 17 (P17) to evaluate neurological damage and functional changes. Western blot analysis of ciliary neurotrophic factor (CNTF), glial cell line-...
Source: Cell Transplantation - November 29, 2023 Category: Cytology Authors: Jifu Xin Lvlv Zhou Ling Zhang Kai Guo Dayong Yang Source Type: research
Neuroprotective Effects of Human Adipose-Derived Mesenchymal Stem Cells in Oxygen-Induced Retinopathy
This study was designed to provide evidence of the neuroprotective of human adipose-derived mesenchymal stem cells (hADSCs) in oxygen-induced retinopathy (OIR). In vivo, hADSCs were intravitreally injected into OIR mice. Various assessments, including HE (histological evaluation), TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) staining, electroretinogram (ERG) analysis, and retinal flat-mount examination, were performed separately at postnatal days 15 (P15) and 17 (P17) to evaluate neurological damage and functional changes. Western blot analysis of ciliary neurotrophic factor (CNTF), glial cell line-...
Source: Cell Transplantation - November 29, 2023 Category: Cytology Authors: Jifu Xin Lvlv Zhou Ling Zhang Kai Guo Dayong Yang Source Type: research
Neuroprotective Effects of Human Adipose-Derived Mesenchymal Stem Cells in Oxygen-Induced Retinopathy
This study was designed to provide evidence of the neuroprotective of human adipose-derived mesenchymal stem cells (hADSCs) in oxygen-induced retinopathy (OIR). In vivo, hADSCs were intravitreally injected into OIR mice. Various assessments, including HE (histological evaluation), TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) staining, electroretinogram (ERG) analysis, and retinal flat-mount examination, were performed separately at postnatal days 15 (P15) and 17 (P17) to evaluate neurological damage and functional changes. Western blot analysis of ciliary neurotrophic factor (CNTF), glial cell line-...
Source: Cell Transplantation - November 29, 2023 Category: Cytology Authors: Jifu Xin Lvlv Zhou Ling Zhang Kai Guo Dayong Yang Source Type: research
Safety and Clinical Effects of a Muse Cell-Based Product in Patients With Amyotrophic Lateral Sclerosis: Results of a Phase 2 Clinical Trial
Cell Transplant. 2023 Jan-Dec;32:9636897231214370. doi: 10.1177/09636897231214370.ABSTRACTAmyotrophic lateral sclerosis (ALS) is characterized by progressive loss of motor neurons. Multilineage-differentiating stress-enduring (Muse) cells are unique endogenous stem cells that show therapeutic effects on motor function in ALS mouse models. We conducted a single-center open phase II clinical trial to evaluate the safety and clinical effects of repeated intravenous injections of an allogenic Muse cell-based product, CL2020, in patients with ALS. Five patients with ALS received CL2020 intravenously once a month for a total of ...
Source: Cell Transplantation - November 28, 2023 Category: Cytology Authors: Toru Yamashita Yumiko Nakano Ryo Sasaki Koh Tadokoro Yoshio Omote Taijun Yunoki Yuko Kawahara Namiko Matsumoto Yuki Taira Chika Matsuoka Ryuta Morihara Koji Abe Source Type: research
Safety and Clinical Effects of a Muse Cell-Based Product in Patients With Amyotrophic Lateral Sclerosis: Results of a Phase 2 Clinical Trial
Cell Transplant. 2023 Jan-Dec;32:9636897231214370. doi: 10.1177/09636897231214370.ABSTRACTAmyotrophic lateral sclerosis (ALS) is characterized by progressive loss of motor neurons. Multilineage-differentiating stress-enduring (Muse) cells are unique endogenous stem cells that show therapeutic effects on motor function in ALS mouse models. We conducted a single-center open phase II clinical trial to evaluate the safety and clinical effects of repeated intravenous injections of an allogenic Muse cell-based product, CL2020, in patients with ALS. Five patients with ALS received CL2020 intravenously once a month for a total of ...
Source: Cell Transplantation - November 28, 2023 Category: Cytology Authors: Toru Yamashita Yumiko Nakano Ryo Sasaki Koh Tadokoro Yoshio Omote Taijun Yunoki Yuko Kawahara Namiko Matsumoto Yuki Taira Chika Matsuoka Ryuta Morihara Koji Abe Source Type: research
LINC01140 Hinders the Development of Breast Cancer Through Targeting miR-200b-3p to Downregulate DMD
Cell Transplant. 2023 Jan-Dec;32:9636897231211202. doi: 10.1177/09636897231211202.ABSTRACTLong non-coding RNAs (lncRNAs) are frequently reported to be involved in breast cancer (BC) oncogenicity. The goal of this study was to probe lncRNA LINC01140's role and action mechanism in BC. Relative LINC01140, miR-200b-3p, and dystrophin (DMD) levels were determined using quantitative real-time polymerase chain reaction (qRT-PCR). DMD protein levels in BC cells were quantified using Western blotting, and the targeting relationships were validated by luciferase reporter assays and RNA immunoprecipitation experiments. The proliferat...
Source: Cell Transplantation - November 27, 2023 Category: Cytology Authors: Gongling Peng Jiaqi Yan Pengfei Shi Hai Li Source Type: research
HIF-1 α-Induced Mitophagy Regulates the Regenerative Outcomes of Stem Cells in Fat Transplantation
Cell Transplant. 2023 Jan-Dec;32:9636897231210750. doi: 10.1177/09636897231210750.ABSTRACTHypoxia is a crucial factor with type diversity that plays an important role in stem cell transplantation. However, the effects of hypoxia on adipose-derived stem cells (ADSCs) are largely unclear in the autologous fat transplantation (AFT) model, which shows a special type of "acute-progressively resolving hypoxia." Here, an AFT model in nude mice and a hypoxic culture model for ADSCs were combined to explore the link between hypoxia-inducible factor-1 α subunit (HIF-1α) and mitophagy under hypoxic conditions. The results showed th...
Source: Cell Transplantation - November 27, 2023 Category: Cytology Authors: Kai Zhang Dan Jin Xin Zhao Bin Lu Weiwei Guo Rui Ren Simo Wu Junrui Zhang Yunpeng Li Source Type: research
Phenotyping of Macrophages After Radiolabeling and Safety of Intra-arterial Transplantation Assessed by SPECT/CT and MRI
In conclusion, activated macrophages are suitable candidates for targeted IA administration without adverse effects on normal, healthy brain parenchyma.PMID:38009543 | DOI:10.1177/09636897231212780 (Source: Cell Transplantation)
Source: Cell Transplantation - November 27, 2023 Category: Cytology Authors: Ida Friberger Vamsi Gontu Robert A Harris Thuy A Tran Johan Lundberg Staffan Holmin Source Type: research
Telocyte-Derived Exosomes Provide an Important Source of Wnts That Inhibits Fibrosis and Supports Regeneration and Repair of Endometrium
This study aims to further investigate the role of TC-derived Wnt ligands carried by exosomes (Exo) in reversal of fibrosis and enhancement of regeneration repair in endometrium. IUA cellular and animal models were established from endometrial stromal cells (ESCs) and mice, followed with treatment of TC-conditioned medium (TCM) or TC-derived Exo. In cellular model, fibrosis markers (collagen type 1 alpha 1 [COL1A1], fibronectin [FN], and α-smooth muscle actin [α-SMA]), angiogenesis (vascular endothelial growth factor [VEGF]), and pathway protein (β-catenin) were determined by quantitative reverse transcription polymeras...
Source: Cell Transplantation - November 25, 2023 Category: Cytology Authors: Tian-Quan Chen Xiao-Jiao Wei Hai-Yan Liu Sheng-Hua Zhan Xiao-Jun Yang Source Type: research
Telocyte-Derived Exosomes Provide an Important Source of Wnts That Inhibits Fibrosis and Supports Regeneration and Repair of Endometrium
This study aims to further investigate the role of TC-derived Wnt ligands carried by exosomes (Exo) in reversal of fibrosis and enhancement of regeneration repair in endometrium. IUA cellular and animal models were established from endometrial stromal cells (ESCs) and mice, followed with treatment of TC-conditioned medium (TCM) or TC-derived Exo. In cellular model, fibrosis markers (collagen type 1 alpha 1 [COL1A1], fibronectin [FN], and α-smooth muscle actin [α-SMA]), angiogenesis (vascular endothelial growth factor [VEGF]), and pathway protein (β-catenin) were determined by quantitative reverse transcription polymeras...
Source: Cell Transplantation - November 25, 2023 Category: Cytology Authors: Tian-Quan Chen Xiao-Jiao Wei Hai-Yan Liu Sheng-Hua Zhan Xiao-Jun Yang Source Type: research
Telocyte-Derived Exosomes Provide an Important Source of Wnts That Inhibits Fibrosis and Supports Regeneration and Repair of Endometrium
This study aims to further investigate the role of TC-derived Wnt ligands carried by exosomes (Exo) in reversal of fibrosis and enhancement of regeneration repair in endometrium. IUA cellular and animal models were established from endometrial stromal cells (ESCs) and mice, followed with treatment of TC-conditioned medium (TCM) or TC-derived Exo. In cellular model, fibrosis markers (collagen type 1 alpha 1 [COL1A1], fibronectin [FN], and α-smooth muscle actin [α-SMA]), angiogenesis (vascular endothelial growth factor [VEGF]), and pathway protein (β-catenin) were determined by quantitative reverse transcription polymeras...
Source: Cell Transplantation - November 25, 2023 Category: Cytology Authors: Tian-Quan Chen Xiao-Jiao Wei Hai-Yan Liu Sheng-Hua Zhan Xiao-Jun Yang Source Type: research
Human Umbilical Cord Mesenchymal Stem Cells Improve the Status of Hypoxic/Ischemic Cerebral Palsy Rats by Downregulating NogoA/NgR/Rho Pathway
This study confirmed that hUCMSC can efficiently improve neurobehavioral status and alleviate brain injury in hypoxia/ischemia-induced CP rat model and HT22 cell model through downregulating the NogoA/NgR/Rho pathway.PMID:37982384 | PMC:PMC10664427 | DOI:10.1177/09636897231210069 (Source: Cell Transplantation)
Source: Cell Transplantation - November 20, 2023 Category: Cytology Authors: Yaoling Luo Jiayang Qu Zhengyi He Minhong Zhang Zhengwei Zou Lincai Li Yuxian Zhang Junsong Ye Source Type: research
