Efficient dissolution of cellulose in slow-cooling alkaline systems and interacting modes between alkali and urea at the molecular level
Carbohydr Res. 2024 Feb 9;536:109054. doi: 10.1016/j.carres.2024.109054. Online ahead of print.ABSTRACTThe dissolution of microcrystalline cellulose (MCC) in a urea-NaOH system is beneficial for its mechanical processing. The apparent MCC solubility was greatly improved to 14 wt% under a slow-cooling condition with a cooling rate of -0.3 °C/min. The cooling curve or thermal history played a crucial role in the dissolution process. An exotherm (-54.7 ± 3 J/g MCC) was detected by DSC only under the slow-cooling condition, and the cryogenic dissolution of MCC was attributed to the exothermic interaction between MCC and solv...
Source: Carbohydrate Research - February 13, 2024 Category: Genetics & Stem Cells Authors: Shuo Ai Zhenhua Huang Wanguo Yu Chengdu Huang Source Type: research
Study the anticancer efficacy of doxorubicin-loaded redox-responsive chitosan-derived nanoparticles in the MDA-MB-231 cell line
This study focuses on the design and evaluation of redox-responsive nanoparticles (NPs) by synthesizing disulfide-containing N-phthaloyl chitosan-SS-methoxy poly(ethylene glycol) (NPC-SS-mPEG) and incorporating the anti-cancer drug doxorubicin into the NPs. The structural features of NPC-SS-mPEG were investigated using FTIR, NMR, XRD, and TGA/DTA analysis. DLS and TEM analysis confirmed the particle size and morphology of the NPs. The stability of the NPs was measured with the presence and absence of glutathione (GSH) in buffers pH 5 and 7.4. Furthermore, the release of DOX from the NPs was studied in GSH (10 mM) containin...
Source: Carbohydrate Research - February 12, 2024 Category: Genetics & Stem Cells Authors: Mariya Gover Antoniraj Yamini Dhayanandamoorthy Kumar Ponnuchamy Ruckmani Kandasamy Kasi Pandima Devi Source Type: research
Trigalacturonate-producing pectate lyase PelQ1 from Saccharobesus litoralis with unique exolytic activity
Carbohydr Res. 2024 Jan 26;536:109045. doi: 10.1016/j.carres.2024.109045. Online ahead of print.ABSTRACTPelQ1 from Saccharobesus litoralis is a Ca2+-dependent pectate lyase belonging to the polysaccharide lyase family 1 (PL1). Although being an endolytic enzyme, it degraded polygalacturonate into predominantly unsaturated trimer in an exolytic manner with delayed production of dimer, tetramer and pentamer. The enzyme harbours a C-terminal domain from the carbohydrate-binding module family 13 (CBM13), whose presence facilitated the production of dimer. PelQ1's homology model showed that it possessed a well-conserved catalyt...
Source: Carbohydrate Research - February 10, 2024 Category: Genetics & Stem Cells Authors: Melissa Qianyue Lian Go Furusawa Aik-Hong Teh Source Type: research
Con A lectin binding by synthetic bivalent arabinomannan tri- and pentasaccharides reveals connectivity-dependent functional valencies
Carbohydr Res. 2024 Feb 1;536:109050. doi: 10.1016/j.carres.2024.109050. Online ahead of print.ABSTRACTLectin Con A, with specificity to interact with α-d-mannopyranoside, achieves tight binding affinity with the aid of optimal multivalent ligand valencies, distances and orientations between the ligands. A series of synthetic arabinomannans, possessing arabinan core and mannan at the non-reducing ends, is studied to assess the above constraints involved with lectin binding in this report. Trisaccharides, with (1 → 2)(1 → 3), (1 → 2)(1 → 5) and (1 → 3)(1 → 5) glycosidic bond connectivities, and a pentasaccharid...
Source: Carbohydrate Research - February 9, 2024 Category: Genetics & Stem Cells Authors: Ipsita Parija Shivender Yadav Narayanaswamy Jayaraman Source Type: research
Extraction, structural characterization and immunoactivity of glucomannan type polysaccahrides from Lilium brownii var. viridulum Baker
In conclusion, LBP played an important role in immunomodulation, and further studies on the specific immunomodulatory mechanisms of LBP on RAW 264.7 cells are still needed.PMID:38335805 | DOI:10.1016/j.carres.2024.109046 (Source: Carbohydrate Research)
Source: Carbohydrate Research - February 9, 2024 Category: Genetics & Stem Cells Authors: Xiao-Jun Li Yuan Yin Shi-Jun Xiao Jiang Chen Rui Zhang Tong Yang Tong-Yu Zhou Si-Yan Zhang Pei Hu Xue Zhang Source Type: research
Structural characterization of a distinct fucan sulfate from Pattalus mollis through an oligosaccharide mapping approach
Carbohydr Res. 2024 Feb 1;536:109052. doi: 10.1016/j.carres.2024.109052. Online ahead of print.ABSTRACTThe elucidation of the precise structure of fucan sulfate is essential for understanding the structure-activity relationship and promoting potential biomedical applications. In this work, the structure of a distinct fucan sulfate fraction V (PmFS in Ref 15 and FSV in Ref 16 → PFV) from Pattalus mollis was investigated using an oligosaccharide mapping approach. Six size-homogeneous fractions were purified from the mild acid hydrolyzed PFV and identified as fucitols, disaccharides and trisaccharides by 1D/2D NMR and MS an...
Source: Carbohydrate Research - February 7, 2024 Category: Genetics & Stem Cells Authors: Yan Ma Zhichuang Zuo Wenqi Zheng Ronghua Yin Xuewen Wu Yujun Ma Mengchen Ji Wenwen Ma Xian Li Weilie Xiao Na Gao Jinhua Zhao Source Type: research
Stereocontrolled synthesis of α-d-allulofuranosides using α-selective d-fructofuranosidation reaction
Carbohydr Res. 2024 Jan 30;536:109044. doi: 10.1016/j.carres.2024.109044. Online ahead of print.ABSTRACTStereocontrolled synthesis of rare sugar derivatives, namely α-d-allulofuranosides, was achieved using d-fructose, one of the most abundant carbohydrates in nature. The following are the key steps of the α-d-allulofuranosides' synthesis. (1) An α-selective glycosidation reaction of 1,3,4,6-tetra-O-benzoylated d-fructofuranosyl donor to obtain α-d-fructofuranosides with 98 %-75 % isolated yields. (2) A regioselective 1,4,6-tri-O-pivaloylation reaction of the tetraol of α-d-fructofuranosides with the C3-hydroxy group ...
Source: Carbohydrate Research - February 7, 2024 Category: Genetics & Stem Cells Authors: Akihiro Iyoshi Yui Makura Masakazu Tanaka Atsushi Ueda Source Type: research
Fe(III)-catalyzed stereoselective synthesis of deoxyglycosides using stable bifunctional deoxy-phenylpropiolate glycoside donors
Carbohydr Res. 2024 Feb 2;536:109051. doi: 10.1016/j.carres.2024.109051. Online ahead of print.ABSTRACTHerein, we report a mild and economical route for the stereoselective synthesis of 2-deoxy and 2,6-dideoxyglycosides via FeCl3-catalyzed activation of bench stable deoxy-phenylpropiolate glycosyl donors (D-PPGs). Optimized reaction conditions work well under additive-free conditions to afford the corresponding 2-deoxy and 2,6-dideoxyglycosides in good yields with high α-anomeric selectivity by reacting with sugar and non-sugar-based acceptors. The optimized conditions were also extended for disarmed D-PPG donors. In addi...
Source: Carbohydrate Research - February 7, 2024 Category: Genetics & Stem Cells Authors: Anjali Aghi Sankar Sau Amit Kumar Source Type: research
Rapid preparation and proton NMR fingerprinting of polysaccharides from Radix Astragali
Carbohydr Res. 2024 Feb 1;536:109053. doi: 10.1016/j.carres.2024.109053. Online ahead of print.ABSTRACTThe purity, content, and structure of the polysaccharides prepared from a specific medicinal plant are the fundamental basis to interpret the observed biological activities. An ultrafiltration-based method has been developed for rapid preparation of total and fractional polysaccharides from Radix Astragali in high yield and purity. This method involves extraction of plant material by hot water, treatment with Sevag reagent, and ultrafiltration using molecular weight cutoff concentrators. The prepared polysaccharides were ...
Source: Carbohydrate Research - February 4, 2024 Category: Genetics & Stem Cells Authors: Liang Cao Jianping Zhao Mei Wang Ikhlas A Khan Xing-Cong Li Source Type: research
Identification and characterization of organic and glycosidic acids in the crude resin glycoside fraction of Ipomoea lacunosa seeds
In this study, the components of the crude resin glycoside fraction extracted from the seeds of I. lacunosa are characterized. Alkaline hydrolysis of the crude resin glycoside fraction obtained from methanolic extract of the seeds yielded three organic acids, namely, 2S-methylbutyric, (E)-2-methylbut-2-enoic, and 2R-methyl-3R-hydroxybutyric acids, and a glycosidic acid fraction. Acidic hydrolysis of the glycosidic acid fraction yielded hydroxyl fatty acid components, including 7S-hydroxydecanoic, 11S-hydroxytetradecanoic, 11S-hydroxyhexadecanoic, 3S,11S-dihydroxytetradecanoic, 3S,11S-dihydroxyhexadecanoic, and 3S,12S-dihyd...
Source: Carbohydrate Research - February 4, 2024 Category: Genetics & Stem Cells Authors: Kazutaka Uemura Renjyu Murakami Eiki Kimura Marina Kai Nodoka Misuda Shin Yasuda Hiroyuki Miyashita Hitoshi Yoshimitsu Ryota Tsuchihasi Masafumi Okawa Junei Kinjo Masateru Ono Source Type: research
Synthesis of sialyl halides with various acyl protective groups
Carbohydr Res. 2024 Jan 9;536:109033. doi: 10.1016/j.carres.2024.109033. Online ahead of print.ABSTRACTGlycosyl halides are historically one of the first glycosyl donors used in glycosylation reactions, and interest in glycosylation reactions involving this class of glycosyl donors is currently increasing. New methods for their activation have been proposed and effective syntheses of oligosaccharides with their participation have been developed. At the same time, the possibilities of using these approaches to the synthesis of sialosides are restricted by the limited diversity of known sialyl halides (previously, mainly sia...
Source: Carbohydrate Research - January 31, 2024 Category: Genetics & Stem Cells Authors: Zarina Z Mamirgova Alexander I Zinin Alexander O Chizhov Leonid O Kononov Source Type: research
Moraxella ovis and Moraxella bovoculi lipooligosaccharide biosynthesis genes, and structural characterisation of oligosaccharide from M. ovis 354T
Carbohydr Res. 2024 Jan 24;536:109043. doi: 10.1016/j.carres.2024.109043. Online ahead of print.ABSTRACTMoraxella ovis is a Gram-negative bacterium isolated from sheep conjunctivitis cases and is a rare isolate of infectious bovine keratoconjunctivitis (IBK). This species is closely related to M. bovoculi, another species which can also be isolated from IBK, or cattle upper respiratory tract (URT). Prior to molecular identification techniques, M. bovoculi was frequently misclassified as M. ovis. We previously described the structure of two oligosaccharides (lipooligosaccharide-derived, minor and major glycoforms) from M. b...
Source: Carbohydrate Research - January 28, 2024 Category: Genetics & Stem Cells Authors: Kosala S Ravikumaran Rebecca M King Anna Notaro Antonio Molinaro Cristina de Castro Jennifer C Wilson I Darren Grice Ian R Peak Source Type: research
Microbial production of N-acetyl-D-glucosamine (GlcNAc) for versatile applications: Biotechnological strategies for green process development
Carbohydr Res. 2024 Jan 17;536:109039. doi: 10.1016/j.carres.2024.109039. Online ahead of print.ABSTRACTN-acetyl-d-glucosamine (GlcNAc) is a commercially important amino sugar for its wide range of applications in pharmaceutical, food, cosmetics and biofuel industries. In nature, GlcNAc is polymerised into chitin biopolymer, which is one of the major constituents of fungal cell wall and outer shells of crustaceans. Sea food processing industries generate a large volume of chitin as biopolymeric waste. Because of its high abundance, chitinaceous shellfish wastes have been exploited as one of the major precursor substrates o...
Source: Carbohydrate Research - January 26, 2024 Category: Genetics & Stem Cells Authors: Sancharini Das Chiranjit Chowdhury S Pavan Kumar Debasis Roy Suresh W Gosavi Ramkrishna Sen Source Type: research
A convenient assay for soluble Dectin-1 lectin domain binding to insoluble β-glucans
Carbohydr Res. 2024 Jan 17;536:109041. doi: 10.1016/j.carres.2024.109041. Online ahead of print.ABSTRACTβ-Glucan is a homopolymer with a backbone of β-1,3-linked glucose residues. The solubility and biological activity of β-glucan can be influenced by the length of the backbone and the length/interval of the β-1,6 branches. Dectin-1 is crucial in innate immunity through its binding to exogenous β-glucans. However, there are few quantitative binding affinities available and there is no comprehensive comparative analysis of the binding of Dectin-1 to insoluble β-glucans. Here, we have developed a simple binding assay f...
Source: Carbohydrate Research - January 23, 2024 Category: Genetics & Stem Cells Authors: Hanako Chiba Noriyoshi Manabe Junko Naito Norihisa Nishida Naohito Ohno Yoshiki Yamaguchi Source Type: research
Selenizing chitooligosaccharide with site-selective modification to alleviate acute liver injury in vivo
Carbohydr Res. 2024 Jan 17;536:109042. doi: 10.1016/j.carres.2024.109042. Online ahead of print.ABSTRACTTwo selenized chitooligosaccharide (O-Se-COS and N,O-Se-COS) with different sites modification were synthesized to alleviate liver injury in vivo. Comparing to traditional COS, both selenized COS exhibited enhanced reducibility as well as antioxidant capacity in vitro. Furthermore, O-Se-COS demonstrated superior efficacy in reducing intracellular reactive oxygen species (ROS) and mitochondrial damage compared to N,O-Se-COS as its enhanced cellular uptake by the positive/negative charge interactions. Two mechanisms were p...
Source: Carbohydrate Research - January 20, 2024 Category: Genetics & Stem Cells Authors: Qiang Chen Lu Wang Sirong Li Dan Lv Xinyi Li Wenting Yin Ting Hu Conghu Li Xu Cheng Source Type: research
