Fabrication of a reusable electrochemical platform based on acid-responsive host-guest interaction with β- cyclodextrin
Carbohydr Res. 2023 Oct 12;534:108966. doi: 10.1016/j.carres.2023.108966. Online ahead of print.ABSTRACTA reusable electrochemical glassy carbon electrode (GCE) platform based on the acid-responsive host-guest interaction between β-cyclodextrin (β-CD) and benzimidazole (BM) derivatives was developed. The β-CD can specifically recognize the BM derivative through the acid -responsive host-guest interaction. The electrode was first modified by eletrografting to immobilize a diamine linker (Boc-EDA), resulting in GCEBoc-EDA in which one amine was used for covalent immobilization to the electrode and another Boc protected am...
Source: Carbohydrate Research - October 16, 2023 Category: Genetics & Stem Cells Authors: Wun Liang Zhi-Jia Chen Chian-Hui Lai Source Type: research
An alternative route for tricyclic quinazolinone-iminosugars and their glucosidase inhibitory activities
Carbohydr Res. 2023 Oct 11;534:108967. doi: 10.1016/j.carres.2023.108967. Online ahead of print.ABSTRACTA series of novel tricyclic quinazolinone-iminosugars 5 and their derivatives 7 were obtained from the tosylated sugars by three steps. Firstly, the reaction of the isopropylidene protected sugar tosylate 1 and o-aminobenzylamine 2 generated the precursor tricyclic quinazolin-iminosuar 3, which was then oxidized by KMnO4 to produce the corresponding quinazolinone 4. Finally, removal of the isopropylidene group yielded the target tricyclic quinazolinone iminosugars 5. In addition, quinazolinone-iminosugars 4ac, 4bc and 4c...
Source: Carbohydrate Research - October 16, 2023 Category: Genetics & Stem Cells Authors: Jie Li Xiaoyan Huang Likai Zhou Song Xie Lulu Su Chao Wei Xiaoliu Li Hua Chen Source Type: research
Fabrication of a reusable electrochemical platform based on acid-responsive host-guest interaction with β- cyclodextrin
Carbohydr Res. 2023 Oct 12;534:108966. doi: 10.1016/j.carres.2023.108966. Online ahead of print.ABSTRACTA reusable electrochemical glassy carbon electrode (GCE) platform based on the acid-responsive host-guest interaction between β-cyclodextrin (β-CD) and benzimidazole (BM) derivatives was developed. The β-CD can specifically recognize the BM derivative through the acid -responsive host-guest interaction. The electrode was first modified by eletrografting to immobilize a diamine linker (Boc-EDA), resulting in GCEBoc-EDA in which one amine was used for covalent immobilization to the electrode and another Boc protected am...
Source: Carbohydrate Research - October 16, 2023 Category: Genetics & Stem Cells Authors: Wun Liang Zhi-Jia Chen Chian-Hui Lai Source Type: research
α-d-(1 → 3)-graft-(1 → 6)-glucan: Comb-like polysaccharide synthesized in vitro with α-1,3/1,6-glucosyltransferase L from Streptococcus salivarius
Carbohydr Res. 2023 Oct 5;534:108969. doi: 10.1016/j.carres.2023.108969. Online ahead of print.ABSTRACTWe demonstrated that a unique polysaccharide with extremely high molecular weight can be easily obtained via a low-cost, mild reaction in a water medium from sucrose, a photosynthetic product. α-1,3/1,6-Glucosyltransferase L (GtfL) from Streptococcus salivarius produced water-insoluble α-d-glucan from sucrose at 37 °C. Gel permeation chromatography revealed the molecular weight was extremely high; the weight-average molecular weight values were more than 1,000,000 irrespective of the substrate concentration. The Smith ...
Source: Carbohydrate Research - October 15, 2023 Category: Genetics & Stem Cells Authors: Ryosuke Kusumi Shinichi Asai Qinfeng He Kayoko Kobayashi Satoshi Kimura Makoto Yoshida Masahisa Wada Source Type: research
α-d-(1 → 3)-graft-(1 → 6)-glucan: Comb-like polysaccharide synthesized in vitro with α-1,3/1,6-glucosyltransferase L from Streptococcus salivarius
Carbohydr Res. 2023 Oct 5;534:108969. doi: 10.1016/j.carres.2023.108969. Online ahead of print.ABSTRACTWe demonstrated that a unique polysaccharide with extremely high molecular weight can be easily obtained via a low-cost, mild reaction in a water medium from sucrose, a photosynthetic product. α-1,3/1,6-Glucosyltransferase L (GtfL) from Streptococcus salivarius produced water-insoluble α-d-glucan from sucrose at 37 °C. Gel permeation chromatography revealed the molecular weight was extremely high; the weight-average molecular weight values were more than 1,000,000 irrespective of the substrate concentration. The Smith ...
Source: Carbohydrate Research - October 15, 2023 Category: Genetics & Stem Cells Authors: Ryosuke Kusumi Shinichi Asai Qinfeng He Kayoko Kobayashi Satoshi Kimura Makoto Yoshida Masahisa Wada Source Type: research
Editorial
Carbohydr Res. 2023 Oct 5:108968. doi: 10.1016/j.carres.2023.108968. Online ahead of print.NO ABSTRACTPMID:37816657 | DOI:10.1016/j.carres.2023.108968 (Source: Carbohydrate Research)
Source: Carbohydrate Research - October 10, 2023 Category: Genetics & Stem Cells Authors: Toshiki Nokami Source Type: research
Editorial
Carbohydr Res. 2023 Oct 5:108968. doi: 10.1016/j.carres.2023.108968. Online ahead of print.NO ABSTRACTPMID:37816657 | DOI:10.1016/j.carres.2023.108968 (Source: Carbohydrate Research)
Source: Carbohydrate Research - October 10, 2023 Category: Genetics & Stem Cells Authors: Toshiki Nokami Source Type: research
Editorial
Carbohydr Res. 2023 Oct 5:108968. doi: 10.1016/j.carres.2023.108968. Online ahead of print.NO ABSTRACTPMID:37816657 | DOI:10.1016/j.carres.2023.108968 (Source: Carbohydrate Research)
Source: Carbohydrate Research - October 10, 2023 Category: Genetics & Stem Cells Authors: Toshiki Nokami Source Type: research
Editorial
Carbohydr Res. 2023 Oct 5:108968. doi: 10.1016/j.carres.2023.108968. Online ahead of print.NO ABSTRACTPMID:37816657 | DOI:10.1016/j.carres.2023.108968 (Source: Carbohydrate Research)
Source: Carbohydrate Research - October 10, 2023 Category: Genetics & Stem Cells Authors: Toshiki Nokami Source Type: research
Probing enzymatic properties of N-glycosyltransferase isoforms from Mannheimia haemolytica
Carbohydr Res. 2023 Sep 19;534:108947. doi: 10.1016/j.carres.2023.108947. Online ahead of print.ABSTRACTN-Glycosyltransferase (NGT) is an inverting glycosyltransferase for an unusual pathway of N-linked protein glycosylation and glycosylates polypeptides in the consensus sequon (N-(X≠P)-T/S) with hexose monosaccharides. Here, we expressed and characterized a novel N-glycosyltransferase from Mannheimia haemolytica (named MhNGT). RP-HPLC and Mass Spectrometry were used to assay and quantify glycopeptide formation by MhNGT and determine its substrate specificities. MhNGT could utilize a variety of nucleotide-activated sugar...
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Zhaoxi Liu Xiaoyu Liu Yongheng Rong Min Chen Source Type: research
Oriented display of cello-oligosaccharides for pull-down binding assays to distinguish binding preferences of glycan binding proteins
Carbohydr Res. 2023 Sep 24;534:108943. doi: 10.1016/j.carres.2023.108943. Online ahead of print.ABSTRACTThe production of biofuels from lignocellulosic biomass using carbohydrate-active enzymes like cellulases is key to a sustainable energy production. Understanding the adsorption mechanism of cellulases and associated binding domain proteins down to the molecular level details will help in the rational design of improved cellulases. In nature, carbohydrate-binding modules (CBMs) from families 17 and 28 often appear in tandem appended to the C-terminus of several endocellulases. Both CBMs are known to bind to the amorphous...
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Markus Hackl Zachary Power Shishir P S Chundawat Source Type: research
Isolation and structural elucidation of prebiotic oligosaccharides from Ziziphi Spinosae Semen
This study explored the structure-activity relationship of raffinose family oligosaccharides (RFOs) and showed that the more the monosaccharide type, the more supportive the gut bacteria growth when oligosaccharides have the same molecular weight.PMID:37783055 | DOI:10.1016/j.carres.2023.108948 (Source: Carbohydrate Research)
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Juan Song Yue Liu Xiangchang Yin Yi Nan Yuhao Shi Xiaojuan Chen Haizhen Liang Jie Zhang Baiping Ma Source Type: research
Probing enzymatic properties of N-glycosyltransferase isoforms from Mannheimia haemolytica
Carbohydr Res. 2023 Sep 19;534:108947. doi: 10.1016/j.carres.2023.108947. Online ahead of print.ABSTRACTN-Glycosyltransferase (NGT) is an inverting glycosyltransferase for an unusual pathway of N-linked protein glycosylation and glycosylates polypeptides in the consensus sequon (N-(X≠P)-T/S) with hexose monosaccharides. Here, we expressed and characterized a novel N-glycosyltransferase from Mannheimia haemolytica (named MhNGT). RP-HPLC and Mass Spectrometry were used to assay and quantify glycopeptide formation by MhNGT and determine its substrate specificities. MhNGT could utilize a variety of nucleotide-activated sugar...
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Zhaoxi Liu Xiaoyu Liu Yongheng Rong Min Chen Source Type: research
Oriented display of cello-oligosaccharides for pull-down binding assays to distinguish binding preferences of glycan binding proteins
Carbohydr Res. 2023 Sep 24;534:108943. doi: 10.1016/j.carres.2023.108943. Online ahead of print.ABSTRACTThe production of biofuels from lignocellulosic biomass using carbohydrate-active enzymes like cellulases is key to a sustainable energy production. Understanding the adsorption mechanism of cellulases and associated binding domain proteins down to the molecular level details will help in the rational design of improved cellulases. In nature, carbohydrate-binding modules (CBMs) from families 17 and 28 often appear in tandem appended to the C-terminus of several endocellulases. Both CBMs are known to bind to the amorphous...
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Markus Hackl Zachary Power Shishir P S Chundawat Source Type: research
Isolation and structural elucidation of prebiotic oligosaccharides from Ziziphi Spinosae Semen
This study explored the structure-activity relationship of raffinose family oligosaccharides (RFOs) and showed that the more the monosaccharide type, the more supportive the gut bacteria growth when oligosaccharides have the same molecular weight.PMID:37783055 | DOI:10.1016/j.carres.2023.108948 (Source: Carbohydrate Research)
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Juan Song Yue Liu Xiangchang Yin Yi Nan Yuhao Shi Xiaojuan Chen Haizhen Liang Jie Zhang Baiping Ma Source Type: research
