Synthesis of a unique mannose α-1-phosphate side chain moiety found in Candida auris cell wall mannan
Carbohydr Res. 2024 Feb 20;537:109059. doi: 10.1016/j.carres.2024.109059. Online ahead of print.ABSTRACTCandida auris is an emerging fungal pathogen that has become a world-wide public health threat. While there have been numerous studies into the nature, composition and structure of the cell wall of Candida albicans and other Candida species, much less is known about the C. auris cell wall. We have shown that C. auris cell wall mannan contains a unique phosphomannan structure which distinguishes C. auris mannan from the mannans found in other fungal species. Specifically, C. auris exhibits two unique acid-labile mannose Î...
Source: Carbohydrate Research - February 26, 2024 Category: Genetics & Stem Cells Authors: Zuchao Ma Harry E Ensley Bridget Graves Michael D Kruppa Peter J Rice Douglas W Lowman David L Williams Source Type: research
Synthesis of a unique mannose α-1-phosphate side chain moiety found in Candida auris cell wall mannan
Carbohydr Res. 2024 Feb 20;537:109059. doi: 10.1016/j.carres.2024.109059. Online ahead of print.ABSTRACTCandida auris is an emerging fungal pathogen that has become a world-wide public health threat. While there have been numerous studies into the nature, composition and structure of the cell wall of Candida albicans and other Candida species, much less is known about the C. auris cell wall. We have shown that C. auris cell wall mannan contains a unique phosphomannan structure which distinguishes C. auris mannan from the mannans found in other fungal species. Specifically, C. auris exhibits two unique acid-labile mannose Î...
Source: Carbohydrate Research - February 26, 2024 Category: Genetics & Stem Cells Authors: Zuchao Ma Harry E Ensley Bridget Graves Michael D Kruppa Peter J Rice Douglas W Lowman David L Williams Source Type: research
Synthesis of a unique mannose α-1-phosphate side chain moiety found in Candida auris cell wall mannan
Carbohydr Res. 2024 Feb 20;537:109059. doi: 10.1016/j.carres.2024.109059. Online ahead of print.ABSTRACTCandida auris is an emerging fungal pathogen that has become a world-wide public health threat. While there have been numerous studies into the nature, composition and structure of the cell wall of Candida albicans and other Candida species, much less is known about the C. auris cell wall. We have shown that C. auris cell wall mannan contains a unique phosphomannan structure which distinguishes C. auris mannan from the mannans found in other fungal species. Specifically, C. auris exhibits two unique acid-labile mannose Î...
Source: Carbohydrate Research - February 26, 2024 Category: Genetics & Stem Cells Authors: Zuchao Ma Harry E Ensley Bridget Graves Michael D Kruppa Peter J Rice Douglas W Lowman David L Williams Source Type: research
The evolving world of milk oligosaccharides: Biochemical diversity understood by computational advances
Carbohydr Res. 2024 Feb 22;537:109069. doi: 10.1016/j.carres.2024.109069. Online ahead of print.ABSTRACTMilk oligosaccharides, complex carbohydrates unique to mammalian milk, play crucial roles in infant nutrition and immune development. This review explores their biochemical diversity, tracing the evolutionary paths that have led to their variation across different species. We highlight the intersection of nutrition, biology, and chemistry in understanding these compounds. Additionally, we discuss the latest computational methods and analytical techniques that have revolutionized the study of milk oligosaccharides, offeri...
Source: Carbohydrate Research - February 25, 2024 Category: Genetics & Stem Cells Authors: Jon Lundstr øm Daniel Bojar Source Type: research
LpxC inhibition: Potential and opportunities with carbohydrate scaffolds
Carbohydr Res. 2024 Feb 12;537:109057. doi: 10.1016/j.carres.2024.109057. Online ahead of print.ABSTRACTUridine diphosphate-3-O-(hydroxymyristoyl)-N-acetylglucosamine deacetylase (LpxC) is a key enzyme involved in the biosynthesis of lipid A, an essential building block, for the construction and assembly of the outer membrane (OM) of Gram-negative bacteria. The enzyme is highly conserved in almost all Gram-negative bacteria and hence has emerged as a promising target for drug discovery in the fight against multi-drug resistant Gram-negative infections. Since the first nanomolar LpxC inhibitor, L-161,240, an oxazoline-based...
Source: Carbohydrate Research - February 25, 2024 Category: Genetics & Stem Cells Authors: Subramanyam Amudala None Sumit Indrapal Singh Aidhen Source Type: research
Structural characterization of an exopolysaccharide produced by two strains of Lacticaseibacilluscasei
Carbohydr Res. 2024 Feb 10;537:109056. doi: 10.1016/j.carres.2024.109056. Online ahead of print.ABSTRACTExopolysaccharides (EPSs) were isolated and purified from Lacticaseibacillus casei strains type V and RW-3703M grown under various fermentation conditions (carbon source, incubation temperature, and duration). Identical 1H NMR spectra were obtained in all cases. The molar mass determined by size-exclusion chromatography coupled with multi-angle light scattering was different for the two strains and in different culture media. The primary structure was elucidated using chemical and spectroscopic techniques. Monosaccharide...
Source: Carbohydrate Research - February 20, 2024 Category: Genetics & Stem Cells Authors: Marie-Rose Van Calsteren Fleur Gagnon Nancy Guertin Denis Roy Source Type: research
Structural characterization of an exopolysaccharide produced by two strains of Lacticaseibacilluscasei
Carbohydr Res. 2024 Feb 10;537:109056. doi: 10.1016/j.carres.2024.109056. Online ahead of print.ABSTRACTExopolysaccharides (EPSs) were isolated and purified from Lacticaseibacillus casei strains type V and RW-3703M grown under various fermentation conditions (carbon source, incubation temperature, and duration). Identical 1H NMR spectra were obtained in all cases. The molar mass determined by size-exclusion chromatography coupled with multi-angle light scattering was different for the two strains and in different culture media. The primary structure was elucidated using chemical and spectroscopic techniques. Monosaccharide...
Source: Carbohydrate Research - February 20, 2024 Category: Genetics & Stem Cells Authors: Marie-Rose Van Calsteren Fleur Gagnon Nancy Guertin Denis Roy Source Type: research
Structural characterization of an exopolysaccharide produced by two strains of Lacticaseibacilluscasei
Carbohydr Res. 2024 Feb 10;537:109056. doi: 10.1016/j.carres.2024.109056. Online ahead of print.ABSTRACTExopolysaccharides (EPSs) were isolated and purified from Lacticaseibacillus casei strains type V and RW-3703M grown under various fermentation conditions (carbon source, incubation temperature, and duration). Identical 1H NMR spectra were obtained in all cases. The molar mass determined by size-exclusion chromatography coupled with multi-angle light scattering was different for the two strains and in different culture media. The primary structure was elucidated using chemical and spectroscopic techniques. Monosaccharide...
Source: Carbohydrate Research - February 20, 2024 Category: Genetics & Stem Cells Authors: Marie-Rose Van Calsteren Fleur Gagnon Nancy Guertin Denis Roy Source Type: research
Structural characterization of an exopolysaccharide produced by two strains of Lacticaseibacilluscasei
Carbohydr Res. 2024 Feb 10;537:109056. doi: 10.1016/j.carres.2024.109056. Online ahead of print.ABSTRACTExopolysaccharides (EPSs) were isolated and purified from Lacticaseibacillus casei strains type V and RW-3703M grown under various fermentation conditions (carbon source, incubation temperature, and duration). Identical 1H NMR spectra were obtained in all cases. The molar mass determined by size-exclusion chromatography coupled with multi-angle light scattering was different for the two strains and in different culture media. The primary structure was elucidated using chemical and spectroscopic techniques. Monosaccharide...
Source: Carbohydrate Research - February 20, 2024 Category: Genetics & Stem Cells Authors: Marie-Rose Van Calsteren Fleur Gagnon Nancy Guertin Denis Roy Source Type: research
Structural characterization of an exopolysaccharide produced by two strains of Lacticaseibacilluscasei
Carbohydr Res. 2024 Feb 10;537:109056. doi: 10.1016/j.carres.2024.109056. Online ahead of print.ABSTRACTExopolysaccharides (EPSs) were isolated and purified from Lacticaseibacillus casei strains type V and RW-3703M grown under various fermentation conditions (carbon source, incubation temperature, and duration). Identical 1H NMR spectra were obtained in all cases. The molar mass determined by size-exclusion chromatography coupled with multi-angle light scattering was different for the two strains and in different culture media. The primary structure was elucidated using chemical and spectroscopic techniques. Monosaccharide...
Source: Carbohydrate Research - February 20, 2024 Category: Genetics & Stem Cells Authors: Marie-Rose Van Calsteren Fleur Gagnon Nancy Guertin Denis Roy Source Type: research
Conformational preferences of guanine-containing threose nucleic acid building blocks in B3LYP studies
Carbohydr Res. 2024 Feb 15;537:109055. doi: 10.1016/j.carres.2024.109055. Online ahead of print.ABSTRACTIn this paper, detailed and systematic gas-phase B3LYP conformational studies of four monomers of threose nucleic acid (TNA) with guanine attached at the C1' atom and bearing different substituents (OH, OP(=O)OH2 and OCH3) in the C2' and C3' positions of the α-l-threofuranose moiety are presented. All exocyclic single-bond (χ, ε and γ) rotations, as well as the ν0-ν4 endocyclic torsion angles, were taken into consideration. Three (threoguanosines TG1-TG3) or two (TG4) energy minima were found for the rotation about...
Source: Carbohydrate Research - February 19, 2024 Category: Genetics & Stem Cells Authors: Justyna Konieczna Karolina Wro Å„ska Marta Kali Å„ska Beata Liberek Andrzej Nowacki Source Type: research
Optimization and degradation studies of cellulose transesterification to palmitate esters in superbase ionic liquid
We examined the influence of reaction temperature, reaction time, and the molar ratio of vinyl palmitate to anhydroglucose unit (AGU) on the DS, which ranged from 0.5 to 2.3 under the given conditions. Notably, the reaction order of the three hydroxy groups was C6-OH > C2-OH > C3-OH. To elucidate the chemical structure of CPs and confirm the transesterification process, various spectroscopic techniques including 1H nuclear magnetic resonance (NMR), 13C NMR, heteronuclear single quantum correlation (HSQC), and solid-state NMR were employed. Higher reaction temperatures and extended reaction times led to a decrease in ...
Source: Carbohydrate Research - February 15, 2024 Category: Genetics & Stem Cells Authors: N Savale E Tarasova I Krasnou M Kudrja Å¡ova V Rjabovs I Reile I Heinmaa A Krumme Source Type: research
Optimization and degradation studies of cellulose transesterification to palmitate esters in superbase ionic liquid
We examined the influence of reaction temperature, reaction time, and the molar ratio of vinyl palmitate to anhydroglucose unit (AGU) on the DS, which ranged from 0.5 to 2.3 under the given conditions. Notably, the reaction order of the three hydroxy groups was C6-OH > C2-OH > C3-OH. To elucidate the chemical structure of CPs and confirm the transesterification process, various spectroscopic techniques including 1H nuclear magnetic resonance (NMR), 13C NMR, heteronuclear single quantum correlation (HSQC), and solid-state NMR were employed. Higher reaction temperatures and extended reaction times led to a decrease in ...
Source: Carbohydrate Research - February 15, 2024 Category: Genetics & Stem Cells Authors: N Savale E Tarasova I Krasnou M Kudrja Å¡ova V Rjabovs I Reile I Heinmaa A Krumme Source Type: research
Optimization and degradation studies of cellulose transesterification to palmitate esters in superbase ionic liquid
We examined the influence of reaction temperature, reaction time, and the molar ratio of vinyl palmitate to anhydroglucose unit (AGU) on the DS, which ranged from 0.5 to 2.3 under the given conditions. Notably, the reaction order of the three hydroxy groups was C6-OH > C2-OH > C3-OH. To elucidate the chemical structure of CPs and confirm the transesterification process, various spectroscopic techniques including 1H nuclear magnetic resonance (NMR), 13C NMR, heteronuclear single quantum correlation (HSQC), and solid-state NMR were employed. Higher reaction temperatures and extended reaction times led to a decrease in ...
Source: Carbohydrate Research - February 15, 2024 Category: Genetics & Stem Cells Authors: N Savale E Tarasova I Krasnou M Kudrja Å¡ova V Rjabovs I Reile I Heinmaa A Krumme Source Type: research
Optimization and degradation studies of cellulose transesterification to palmitate esters in superbase ionic liquid
We examined the influence of reaction temperature, reaction time, and the molar ratio of vinyl palmitate to anhydroglucose unit (AGU) on the DS, which ranged from 0.5 to 2.3 under the given conditions. Notably, the reaction order of the three hydroxy groups was C6-OH > C2-OH > C3-OH. To elucidate the chemical structure of CPs and confirm the transesterification process, various spectroscopic techniques including 1H nuclear magnetic resonance (NMR), 13C NMR, heteronuclear single quantum correlation (HSQC), and solid-state NMR were employed. Higher reaction temperatures and extended reaction times led to a decrease in ...
Source: Carbohydrate Research - February 15, 2024 Category: Genetics & Stem Cells Authors: N Savale E Tarasova I Krasnou M Kudrja Å¡ova V Rjabovs I Reile I Heinmaa A Krumme Source Type: research
