Probing enzymatic properties of N-glycosyltransferase isoforms from Mannheimia haemolytica
Carbohydr Res. 2023 Sep 19;534:108947. doi: 10.1016/j.carres.2023.108947. Online ahead of print.ABSTRACTN-Glycosyltransferase (NGT) is an inverting glycosyltransferase for an unusual pathway of N-linked protein glycosylation and glycosylates polypeptides in the consensus sequon (N-(X≠P)-T/S) with hexose monosaccharides. Here, we expressed and characterized a novel N-glycosyltransferase from Mannheimia haemolytica (named MhNGT). RP-HPLC and Mass Spectrometry were used to assay and quantify glycopeptide formation by MhNGT and determine its substrate specificities. MhNGT could utilize a variety of nucleotide-activated sugar...
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Zhaoxi Liu Xiaoyu Liu Yongheng Rong Min Chen Source Type: research
Oriented display of cello-oligosaccharides for pull-down binding assays to distinguish binding preferences of glycan binding proteins
Carbohydr Res. 2023 Sep 24;534:108943. doi: 10.1016/j.carres.2023.108943. Online ahead of print.ABSTRACTThe production of biofuels from lignocellulosic biomass using carbohydrate-active enzymes like cellulases is key to a sustainable energy production. Understanding the adsorption mechanism of cellulases and associated binding domain proteins down to the molecular level details will help in the rational design of improved cellulases. In nature, carbohydrate-binding modules (CBMs) from families 17 and 28 often appear in tandem appended to the C-terminus of several endocellulases. Both CBMs are known to bind to the amorphous...
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Markus Hackl Zachary Power Shishir P S Chundawat Source Type: research
Isolation and structural elucidation of prebiotic oligosaccharides from Ziziphi Spinosae Semen
This study explored the structure-activity relationship of raffinose family oligosaccharides (RFOs) and showed that the more the monosaccharide type, the more supportive the gut bacteria growth when oligosaccharides have the same molecular weight.PMID:37783055 | DOI:10.1016/j.carres.2023.108948 (Source: Carbohydrate Research)
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Juan Song Yue Liu Xiangchang Yin Yi Nan Yuhao Shi Xiaojuan Chen Haizhen Liang Jie Zhang Baiping Ma Source Type: research
Probing enzymatic properties of N-glycosyltransferase isoforms from Mannheimia haemolytica
Carbohydr Res. 2023 Sep 19;534:108947. doi: 10.1016/j.carres.2023.108947. Online ahead of print.ABSTRACTN-Glycosyltransferase (NGT) is an inverting glycosyltransferase for an unusual pathway of N-linked protein glycosylation and glycosylates polypeptides in the consensus sequon (N-(X≠P)-T/S) with hexose monosaccharides. Here, we expressed and characterized a novel N-glycosyltransferase from Mannheimia haemolytica (named MhNGT). RP-HPLC and Mass Spectrometry were used to assay and quantify glycopeptide formation by MhNGT and determine its substrate specificities. MhNGT could utilize a variety of nucleotide-activated sugar...
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Zhaoxi Liu Xiaoyu Liu Yongheng Rong Min Chen Source Type: research
Oriented display of cello-oligosaccharides for pull-down binding assays to distinguish binding preferences of glycan binding proteins
Carbohydr Res. 2023 Sep 24;534:108943. doi: 10.1016/j.carres.2023.108943. Online ahead of print.ABSTRACTThe production of biofuels from lignocellulosic biomass using carbohydrate-active enzymes like cellulases is key to a sustainable energy production. Understanding the adsorption mechanism of cellulases and associated binding domain proteins down to the molecular level details will help in the rational design of improved cellulases. In nature, carbohydrate-binding modules (CBMs) from families 17 and 28 often appear in tandem appended to the C-terminus of several endocellulases. Both CBMs are known to bind to the amorphous...
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Markus Hackl Zachary Power Shishir P S Chundawat Source Type: research
Isolation and structural elucidation of prebiotic oligosaccharides from Ziziphi Spinosae Semen
This study explored the structure-activity relationship of raffinose family oligosaccharides (RFOs) and showed that the more the monosaccharide type, the more supportive the gut bacteria growth when oligosaccharides have the same molecular weight.PMID:37783055 | DOI:10.1016/j.carres.2023.108948 (Source: Carbohydrate Research)
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Juan Song Yue Liu Xiangchang Yin Yi Nan Yuhao Shi Xiaojuan Chen Haizhen Liang Jie Zhang Baiping Ma Source Type: research
Probing enzymatic properties of N-glycosyltransferase isoforms from Mannheimia haemolytica
Carbohydr Res. 2023 Sep 19;534:108947. doi: 10.1016/j.carres.2023.108947. Online ahead of print.ABSTRACTN-Glycosyltransferase (NGT) is an inverting glycosyltransferase for an unusual pathway of N-linked protein glycosylation and glycosylates polypeptides in the consensus sequon (N-(X≠P)-T/S) with hexose monosaccharides. Here, we expressed and characterized a novel N-glycosyltransferase from Mannheimia haemolytica (named MhNGT). RP-HPLC and Mass Spectrometry were used to assay and quantify glycopeptide formation by MhNGT and determine its substrate specificities. MhNGT could utilize a variety of nucleotide-activated sugar...
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Zhaoxi Liu Xiaoyu Liu Yongheng Rong Min Chen Source Type: research
Oriented display of cello-oligosaccharides for pull-down binding assays to distinguish binding preferences of glycan binding proteins
Carbohydr Res. 2023 Sep 24;534:108943. doi: 10.1016/j.carres.2023.108943. Online ahead of print.ABSTRACTThe production of biofuels from lignocellulosic biomass using carbohydrate-active enzymes like cellulases is key to a sustainable energy production. Understanding the adsorption mechanism of cellulases and associated binding domain proteins down to the molecular level details will help in the rational design of improved cellulases. In nature, carbohydrate-binding modules (CBMs) from families 17 and 28 often appear in tandem appended to the C-terminus of several endocellulases. Both CBMs are known to bind to the amorphous...
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Markus Hackl Zachary Power Shishir P S Chundawat Source Type: research
Isolation and structural elucidation of prebiotic oligosaccharides from Ziziphi Spinosae Semen
This study explored the structure-activity relationship of raffinose family oligosaccharides (RFOs) and showed that the more the monosaccharide type, the more supportive the gut bacteria growth when oligosaccharides have the same molecular weight.PMID:37783055 | DOI:10.1016/j.carres.2023.108948 (Source: Carbohydrate Research)
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Juan Song Yue Liu Xiangchang Yin Yi Nan Yuhao Shi Xiaojuan Chen Haizhen Liang Jie Zhang Baiping Ma Source Type: research
Probing enzymatic properties of N-glycosyltransferase isoforms from Mannheimia haemolytica
Carbohydr Res. 2023 Sep 19;534:108947. doi: 10.1016/j.carres.2023.108947. Online ahead of print.ABSTRACTN-Glycosyltransferase (NGT) is an inverting glycosyltransferase for an unusual pathway of N-linked protein glycosylation and glycosylates polypeptides in the consensus sequon (N-(X≠P)-T/S) with hexose monosaccharides. Here, we expressed and characterized a novel N-glycosyltransferase from Mannheimia haemolytica (named MhNGT). RP-HPLC and Mass Spectrometry were used to assay and quantify glycopeptide formation by MhNGT and determine its substrate specificities. MhNGT could utilize a variety of nucleotide-activated sugar...
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Zhaoxi Liu Xiaoyu Liu Yongheng Rong Min Chen Source Type: research
Oriented display of cello-oligosaccharides for pull-down binding assays to distinguish binding preferences of glycan binding proteins
Carbohydr Res. 2023 Sep 24;534:108943. doi: 10.1016/j.carres.2023.108943. Online ahead of print.ABSTRACTThe production of biofuels from lignocellulosic biomass using carbohydrate-active enzymes like cellulases is key to a sustainable energy production. Understanding the adsorption mechanism of cellulases and associated binding domain proteins down to the molecular level details will help in the rational design of improved cellulases. In nature, carbohydrate-binding modules (CBMs) from families 17 and 28 often appear in tandem appended to the C-terminus of several endocellulases. Both CBMs are known to bind to the amorphous...
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Markus Hackl Zachary Power Shishir P S Chundawat Source Type: research
Isolation and structural elucidation of prebiotic oligosaccharides from Ziziphi Spinosae Semen
This study explored the structure-activity relationship of raffinose family oligosaccharides (RFOs) and showed that the more the monosaccharide type, the more supportive the gut bacteria growth when oligosaccharides have the same molecular weight.PMID:37783055 | DOI:10.1016/j.carres.2023.108948 (Source: Carbohydrate Research)
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Juan Song Yue Liu Xiangchang Yin Yi Nan Yuhao Shi Xiaojuan Chen Haizhen Liang Jie Zhang Baiping Ma Source Type: research
Probing enzymatic properties of N-glycosyltransferase isoforms from Mannheimia haemolytica
Carbohydr Res. 2023 Sep 19;534:108947. doi: 10.1016/j.carres.2023.108947. Online ahead of print.ABSTRACTN-Glycosyltransferase (NGT) is an inverting glycosyltransferase for an unusual pathway of N-linked protein glycosylation and glycosylates polypeptides in the consensus sequon (N-(X≠P)-T/S) with hexose monosaccharides. Here, we expressed and characterized a novel N-glycosyltransferase from Mannheimia haemolytica (named MhNGT). RP-HPLC and Mass Spectrometry were used to assay and quantify glycopeptide formation by MhNGT and determine its substrate specificities. MhNGT could utilize a variety of nucleotide-activated sugar...
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Zhaoxi Liu Xiaoyu Liu Yongheng Rong Min Chen Source Type: research
Oriented display of cello-oligosaccharides for pull-down binding assays to distinguish binding preferences of glycan binding proteins
Carbohydr Res. 2023 Sep 24;534:108943. doi: 10.1016/j.carres.2023.108943. Online ahead of print.ABSTRACTThe production of biofuels from lignocellulosic biomass using carbohydrate-active enzymes like cellulases is key to a sustainable energy production. Understanding the adsorption mechanism of cellulases and associated binding domain proteins down to the molecular level details will help in the rational design of improved cellulases. In nature, carbohydrate-binding modules (CBMs) from families 17 and 28 often appear in tandem appended to the C-terminus of several endocellulases. Both CBMs are known to bind to the amorphous...
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Markus Hackl Zachary Power Shishir P S Chundawat Source Type: research
Isolation and structural elucidation of prebiotic oligosaccharides from Ziziphi Spinosae Semen
This study explored the structure-activity relationship of raffinose family oligosaccharides (RFOs) and showed that the more the monosaccharide type, the more supportive the gut bacteria growth when oligosaccharides have the same molecular weight.PMID:37783055 | DOI:10.1016/j.carres.2023.108948 (Source: Carbohydrate Research)
Source: Carbohydrate Research - October 2, 2023 Category: Genetics & Stem Cells Authors: Juan Song Yue Liu Xiangchang Yin Yi Nan Yuhao Shi Xiaojuan Chen Haizhen Liang Jie Zhang Baiping Ma Source Type: research
