Biochar pH reduction using elemental sulfur and biological activation using compost or vermicompost
This study aimed to improve biochar's quality for arid land applications by using elemental sulfur as a pH reducer agent co-applied with compost or vermicompost as biological activators. Biochar pH was decreased by the addition of elemental sulfur, with the highest reduction from 8.1 to 7.2 occurring when co-amended with vermicompost. Elemental sulfur increased the water-soluble concentrations of calcium, magnesium, and many other elements, and stimulated substrate-induced respiration, especially when co-amended with vermicompost. The bacterial diversity community structure were significantly affected by all treatments. Th...
Source: Bioresource Technology - April 25, 2024 Category: Biotechnology Authors: Ahmed Al-Rabaiai Daniel Menezes-Blackburn Said Al-Ismaily Rhonda Janke Ahmed Al-Alawi Mohamed Al-Kindi Roland Bol Source Type: research
Responses of physiological, microbiome and lipid metabolism to lignocellulose wastes in gut of yellow mealworm (Tenebrio molitor)
This study has selected two types of lignocellulosic wastes, distillers' grains (DG) and maize straw (MS), to feed yellow mealworms. This study investigated the effects of lignocellulosic wastes on the growth, antioxidant system, microbiome, and lipidome of yellow mealworms. The relative growth of lignocellulosic waste group was not significantly different from wheat bran. The antioxidant level was elevated in DG. MS was significantly enriched in cellulose-degrading bacteria in the gut and was accompanied by disturbances in lipid metabolism. The correlation coefficients were used to construct a network connecting diet, mic...
Source: Bioresource Technology - April 25, 2024 Category: Biotechnology Authors: Xinrui Mao Jiaming Li Enqing Meng Wenbiao Jin Wei Han Source Type: research
Biochar pH reduction using elemental sulfur and biological activation using compost or vermicompost
This study aimed to improve biochar's quality for arid land applications by using elemental sulfur as a pH reducer agent co-applied with compost or vermicompost as biological activators. Biochar pH was decreased by the addition of elemental sulfur, with the highest reduction from 8.1 to 7.2 occurring when co-amended with vermicompost. Elemental sulfur increased the water-soluble concentrations of calcium, magnesium, and many other elements, and stimulated substrate-induced respiration, especially when co-amended with vermicompost. The bacterial diversity community structure were significantly affected by all treatments. Th...
Source: Bioresource Technology - April 25, 2024 Category: Biotechnology Authors: Ahmed Al-Rabaiai Daniel Menezes-Blackburn Said Al-Ismaily Rhonda Janke Ahmed Al-Alawi Mohamed Al-Kindi Roland Bol Source Type: research
Responses of physiological, microbiome and lipid metabolism to lignocellulose wastes in gut of yellow mealworm (Tenebrio molitor)
This study has selected two types of lignocellulosic wastes, distillers' grains (DG) and maize straw (MS), to feed yellow mealworms. This study investigated the effects of lignocellulosic wastes on the growth, antioxidant system, microbiome, and lipidome of yellow mealworms. The relative growth of lignocellulosic waste group was not significantly different from wheat bran. The antioxidant level was elevated in DG. MS was significantly enriched in cellulose-degrading bacteria in the gut and was accompanied by disturbances in lipid metabolism. The correlation coefficients were used to construct a network connecting diet, mic...
Source: Bioresource Technology - April 25, 2024 Category: Biotechnology Authors: Xinrui Mao Jiaming Li Enqing Meng Wenbiao Jin Wei Han Source Type: research
Feasibility of in-situ sludge fermentation coupled with partial denitrification: Key roles of initial organic matters and alkaline pH
This study proposed an In-situ Sludge Fermentation coupled with Partial Denitrification (ISFPD) system and explored its performance under different conditions, including initial pH, nitrate concentration, and organic matters. Results showed that nitrite production increased with the elevation of initial pH (from 6 to 9), and the highest nitrate-to-nitrite transformation ratio (NTR) reached 77% at initial pH 9. The PD rates and NTR were observed to be minimally influenced by initial nitrate concentration. Acetate was preferred by denitrifying bacteria, while macromolecules such as proteins necessitated be hydrolyzed to be s...
Source: Bioresource Technology - April 24, 2024 Category: Biotechnology Authors: Lu Wang Xiang Hao Tan Jiang Xiaodi Li Jiayi Yang Bo Wang Source Type: research
Mitochondrial alternative oxidase pathway accelerates non-motile cell germination by enhancing respiratory carbon metabolism and maintaining redox poise in haematococcus pluvialis
Bioresour Technol. 2024 Apr 22:130729. doi: 10.1016/j.biortech.2024.130729. Online ahead of print.ABSTRACTLow efficiency of the cultivation process is a major obstacle in the commercial production of Haematococcus pluvialis. Germination of red, non-motile cells is an efficient strategy for rapid acquisition of zoospores. However, the regulatory mechanisms associated with germination remain unexplored. In the present study, it was confirmed that the mitochondrial alternative oxidase (AOX) pathway accelerates H. pluvialis cell germination, and the regulatory mechanisms were clarified. When the AOX pathway was inhibited, the ...
Source: Bioresource Technology - April 24, 2024 Category: Biotechnology Authors: Jing Li Litao Zhang Wenjie Yu Mengjie Zhang Feng Chen Jianguo Liu Source Type: research
fOne pot synthesis of furan-modified lignin from agricultural waste via lignin-first approach
This study investigated a lignin-first approach to produce furan-modified lignin from sugarcane bagasse (SB), rice hull (RH), and sunn hemp biomass (SHB) using 5 methylfurfural (MF) and 5 methul-2-furanmethanol (MFM). The reaction time (5 h) was selected based on the delignification of SB using methanol and Ru/C catalyst which yielded the highest hydroxyl content. Delignification of SB with various MF weight ratios (1:1, 1:2, 1:3, 2:1, and 3:1) revealed that 1:1 and 2:1 ratios produced the highest hydroxyl content (7.7 mmol/g) and bio-oil yield (23.2 % wt% total weight). Further exploration identified that RH and MF at 1:1...
Source: Bioresource Technology - April 24, 2024 Category: Biotechnology Authors: Loku Mannage N Lakmini Athukoralage Don K Deshan John Bartley Darryn Rackemann Lalehvash Moghaddam Source Type: research
Revealing bioresponses of biofilm and flocs to salinity gradient in halophilic biofilm reactor
This study investigated performance, dynamics, and community assembly of biofilm system under 3 %-7% salinity gradient. The removal efficiency of NH4+-N remained stable and exceeded 93 % at 3 %-6% salinity, but decreased to below 80 % at 7 % salinity. The elevated salinity promoted the synthesis of extracellular polymer substrates, inhibited microbial respiration, and significantly regulated the microbial community structure. Compared to flocs, biofilm exhibited greater species diversity and richer Nitrosomonas. It was found diffusion limitations dominated the microbial community assembly under the salinity gradient. And m...
Source: Bioresource Technology - April 21, 2024 Category: Biotechnology Authors: Weizhi Zhou Jie Hao Yiting Guo Chuanfu Zhao Mengru Zhang Shuhui Zhang Fei Han Source Type: research
Revealing bioresponses of biofilm and flocs to salinity gradient in halophilic biofilm reactor
This study investigated performance, dynamics, and community assembly of biofilm system under 3 %-7% salinity gradient. The removal efficiency of NH4+-N remained stable and exceeded 93 % at 3 %-6% salinity, but decreased to below 80 % at 7 % salinity. The elevated salinity promoted the synthesis of extracellular polymer substrates, inhibited microbial respiration, and significantly regulated the microbial community structure. Compared to flocs, biofilm exhibited greater species diversity and richer Nitrosomonas. It was found diffusion limitations dominated the microbial community assembly under the salinity gradient. And m...
Source: Bioresource Technology - April 21, 2024 Category: Biotechnology Authors: Weizhi Zhou Jie Hao Yiting Guo Chuanfu Zhao Mengru Zhang Shuhui Zhang Fei Han Source Type: research
Efficient production of High-Purity manno-oligosaccharides from guar gum by citric acid and enzymatic hydrolysis
Bioresour Technol. 2024 Apr 18:130719. doi: 10.1016/j.biortech.2024.130719. Online ahead of print.ABSTRACTCurrently, the production of manno-oligosaccharides (MOS) from guar gum faces challenges of low oligosaccharide enzymatic hydrolysis yield and complicated steps in separation and purification. In this work, a potential strategy to address these issues was explored. By combining citric acid pretreatment (300 mM, 130 °C, 1 h) with β-mannanase hydrolysis, an impressive MOS yield of 61.8 % from guar gum (10 %, w/v) was achieved. The key success lay in the optimizing conditions that completely degraded other galactomannan...
Source: Bioresource Technology - April 20, 2024 Category: Biotechnology Authors: Donglin Xin Hong Yin Ganqiao Ran Source Type: research
Deciphering styrene oxide tolerance mechanisms in Gluconobacter oxydans mutant strain
This study provides new insights into the mechanisms underlying the toxicological response to OS stress and offers potential targets for enhancing the solvent tolerance of G. oxydans.PMID:38642663 | DOI:10.1016/j.biortech.2024.130674 (Source: Bioresource Technology)
Source: Bioresource Technology - April 20, 2024 Category: Biotechnology Authors: Yan Chen Fei Liu Aobo Sha Meijuan Xu Zhiming Rao Xian Zhang Source Type: research
Competitive partitioning of denitrification pathways during arrested methanogenesis: Implications in ammonium recovery, N < sub > 2 < /sub > O emission, and volatile fatty acid production
This study investigated the competitive distribution between conventional denitrification (DEN) and dissimilatory nitrate reduction to ammonium (DNRA) during simultaneous denitrification and fermentation in arrested methanogenesis. Up to 62 % of initial NO3- (200 mg-N/L) was retained as ammonium through DNRA at a chemical oxygen demand (COD)/N ratio of 25. Significant N2O emission occurred (1.7 - 8.0 % of the initial NO3-) with limited carbon supply (≤1600 mg COD/L) and sludge concentration (≤3000 mg COD/L). VFA composition shifted predominantly towards acetic acid (>50 %) in the presence of nitrate. A novel kinetic...
Source: Bioresource Technology - April 20, 2024 Category: Biotechnology Authors: Rahamat Ullah Tanvir Yebo Li Zhiqiang Hu Source Type: research
Continuous microalgal culture module and method of culturing microalgae containing macular pigment
This study established and investigated continuous macular pigment (MP) production with a lutein (L):zeaxanthin (Z) ratio of 4-5:1 by an MP-rich Chlorella sp. CN6 mutant strain in a continuous microalgal culture module. Chlorella sp. CN6 was cultured in a four-stage module for 10 days. The microalgal culture volume increased to 200 L in the first stage (6 days). Biomass productivity increased to 0.931 g/L/day with continuous indoor white light irradiation during the second stage (3 days). MP content effectively increased to 8.29 mg/g upon continuous, indoor white light and blue light-emitting diode irradiation in the third...
Source: Bioresource Technology - April 19, 2024 Category: Biotechnology Authors: Meng-Wei Lin Chih-Sheng Lin Yu-Tso Chen Shao-Qian Huang Yi-Chun Yang Wen-Xin Zhang Wei-Hong Chiu Cheng-Han Lin Chiu-Mei Kuo Source Type: research
Structural properties and adsorption performance relationship towards three categories of lignin and their derived biochar
This study focused on three types of lignin and their corresponding biochar derivatives. Among them, ZnCl2-activated acidic/alkali densified lignin preparation of lignin-derived active carbon exhibited superior adsorption performance, achieving 526.32 mg/g for methylene blue and 2156.77 mg/g for congo red. Its exceptional adsorption capacity was attributed to its unique structural properties, including low alkyl and O-alkyl group content and high aromatic carbon levels. Furthermore, the adsorption mechanisms adhered to pseudo-second-order kinetics and the Langmuir model, signifying a spontaneous process. Intriguingly, lign...
Source: Bioresource Technology - April 19, 2024 Category: Biotechnology Authors: Mingyang Hu Jiangwei Chen Yun Liu Source Type: research
Application of multiple strategies to enhance oleanolic acid biosynthesis by engineered Saccharomyces cerevisiae
Bioresour Technol. 2024 Apr 17:130716. doi: 10.1016/j.biortech.2024.130716. Online ahead of print.ABSTRACTOleanolic acid and its derivatives are widely used in the pharmaceutical, agricultural, cosmetic and food industries. Previous studies have shown that oleanolic acid production levels in engineered cell factories are low, which is why oleanolic acid is still widely extracted from traditional medicinal plants. To construct a highly efficient oleanolic acid production strain, rate-limiting steps were regulated by inducible promoters and the expression of key genes in the oleanolic acid synthetic pathway was enhanced. Sub...
Source: Bioresource Technology - April 19, 2024 Category: Biotechnology Authors: Xu Cheng Yaru Pang Yali Ban Shuai Cui Tao Shu Bo Lv Chun Li Source Type: research
