Isolation of ammonium bicarbonate by reactive distillation of food waste digestate liquor
Bioresour Technol. 2024 Mar 20;399:130592. doi: 10.1016/j.biortech.2024.130592. Online ahead of print.ABSTRACTDistillation is among the best techniques for management of ammoniacal nitrogen in anaerobic digestate; however, the suitability of the conventional system is neglected in favor of using more advanced setups. This investigation proves the reliability of the classical batch distillation apparatus for high throughput separation of solid crystals of ammonium bicarbonate from food waste digestate (FWD) liquor. Three replicates were carried out: 80 g of FWD liquor with a content of 50 g/L NH4HCO3 was processed for 7.5 h...
Source: Bioresource Technology - March 22, 2024 Category: Biotechnology Authors: Alejandro Moure Abelenda Source Type: research

New insights into nitrogen removal by divalent iron-enhanced moving bed biofilm reactor: Performance, interfacial interaction and co-occurrence network
In conclusion, iron redox cycling significantly enhanced the nitrogen removal. This study proposes a viable strategy for the efficient treatment of nutrient wastewater.PMID:38518879 | DOI:10.1016/j.biortech.2024.130621 (Source: Bioresource Technology)
Source: Bioresource Technology - March 22, 2024 Category: Biotechnology Authors: Mengcheng Sheng Zhiqing Ye Fang Zhu Dandan Pan Sicheng Shao Xiangwei Wu Source Type: research

Effects of simulated microgravity on current generation of electrochemically active bacteria: Insights from case-control study using random positioning machine
This study first revealed the enrichment in EAB abundance, the increase in EAB biomass, and the promotion of current generation under simulated microgravity.PMID:38518880 | DOI:10.1016/j.biortech.2024.130618 (Source: Bioresource Technology)
Source: Bioresource Technology - March 22, 2024 Category: Biotechnology Authors: Yue Yi Ziyue Zhao Bo Cao Xuemei Yi Zhipeng Mao Fan Zha Zhe Zhang Hong Liu Aiqin Luo Source Type: research

High rates of nitrogen removal in aerated VFCWs treating sewage through C-N-S cycle
Bioresour Technol. 2024 Mar 20:130620. doi: 10.1016/j.biortech.2024.130620. Online ahead of print.ABSTRACTThe efficiency of deep aerated vertical flow constructed wetlands (DA-VFCWs) being operated in Hyderabad, India, was evaluated herein using physicochemical analysis and 16S rRNA amplicon sequencing. The results showed 2-4-fold higher removal rate coefficients for Biochemical oxygen demand (1.32---3.53 m/d) and nitrogen (0.88--1.36 m/d) in DA-VFCWs than those of passive VFCWs. Elevated sulfate concentration in the DA-VFCWs effluent (84-113 mg/L) indicated possibility of sulfur-driven autotrophic denitrification (SDAD) a...
Source: Bioresource Technology - March 22, 2024 Category: Biotechnology Authors: Abhishek Soti Niha Mohan Kulshreshtha Saurabh Singh Akshat Samaria Urmila Brighu Gangadhara Dontireddy Sravan Banda Akhilendra Bhushan Gupta Source Type: research

Modelling of anaerobic digestion of microalgae biomass: Effect of overloading perturbation
Bioresour Technol. 2024 Mar 20:130625. doi: 10.1016/j.biortech.2024.130625. Online ahead of print.ABSTRACTAnaerobic digestion (AD) of microalgae is an intriguing approach for bioenergy production. The scaling-up of AD presents a significant challenge due to the systematic efficiency losses related to process instabilities. To gain a comprehensive understanding of AD behavior, this study assessed a modified version of the anaerobic digestion model No1 (ADM1) + Contois kinetics to represent microalgae AD impacted by overloading. To this end, two new inhibition functions were implemented: inhibition by acetate for acidogenesi...
Source: Bioresource Technology - March 22, 2024 Category: Biotechnology Authors: Silvia Greses Julie Jimenez Cristina Gonz ález-Fernández Jean-Philippe Steyer Source Type: research

Effects of culture temperature and light regimes on biomass and lipid accumulation of Chlamydomonas reinhardtii under carbon-rich and nitrogen-limited conditions
This study investigated the impacts of various culture temperatures and light regimes on growth and biochemical constituents of Chlamydomonas reinhardtii under carbon-supply and nitrogen-limited conditions to improve oil production in algal cells. Results displayed that under a 30 ℃ and 150 μE/m2/s regime, there was a significant increase in biomass, total lipids, and lipid productivity. Specifically, these parameters reached 1.83 g/L, 36.25 %, and 130.73 mg/L/d, respectively. Remarkably, prolonging the photoperiod further enhanced the aforementioned three parameters, reaching peak levels of 1.92 g/L, 41.10 %, and 157.5...
Source: Bioresource Technology - March 21, 2024 Category: Biotechnology Authors: Shiyan Zheng Shourui Sun Shangyun Zou Jiamei Song Lan Hua Hui Chen Qiang Wang Source Type: research

Phosphate additives promote humic acid carbon and nitrogen skeleton formation by regulating precursors and composting bacterial communities
This study aimed to compare the effect of different phosphate additives including superphosphate (CP) and MP [Mg(OH)2 + H3PO4] on nitrogen conversion, humus fractions formation and bacterial community in food waste compost. The results showed the ratio of humic acid nitrogen in total nitrogen (HA-N/TN) in CP increased by 49 %. Ammonium nitrogen accumulation was increased by 75 % (CP) and 44 % (MP). Spectroscopic techniques proved that phosphate addition facilitated the formation of complex structures in HA. CP enhanced the dominance of Saccharomonospora, while Thermobifida and Bacillus were improved in MP. Structural equat...
Source: Bioresource Technology - March 21, 2024 Category: Biotechnology Authors: Jing Su Yabin Zhan Yuan Chang Su Chang Yan Luo Peizhen Chen Xingling Tao Yunfeng Chen Li Yang Ting Xu Yuhui Qiao Ji Li Yuquan Wei Source Type: research

Advanced nitrogen and phosphorus removal in pilot-scale anaerobic/aerobic/anoxic system for municipal wastewater in Northern China
This study has significant implications for the practical application of the AOA-SBR process.PMID:38513924 | DOI:10.1016/j.biortech.2024.130616 (Source: Bioresource Technology)
Source: Bioresource Technology - March 21, 2024 Category: Biotechnology Authors: Changkun Gu Xiyao Li Shujun Zhang Jianwei Li Xiaoyu Gao Guo Chen Zhibin Wang Yongzhen Peng Source Type: research

Exploiting synthetic biology platforms for enhanced biosynthesis of natural products in Yarrowia lipolytica
Bioresour Technol. 2024 Mar 19:130614. doi: 10.1016/j.biortech.2024.130614. Online ahead of print.ABSTRACTWith the rapid development of synthetic biology, researchers can design, modify, or even synthesize microorganisms de novo, and microorganisms endowed with unnatural functions can be considered "artificial life" and facilitate the development of functional products. Based on this concept, researchers can solve critical problems related to the insufficient supply of natural products, such as low yields, long production cycles, and cumbersome procedures. Due to its superior performance and unique physiological and bioche...
Source: Bioresource Technology - March 21, 2024 Category: Biotechnology Authors: Dahai Jiang Manqi Yang Kai Chen Wenxuan Jiang Liangliang Zhang Xiaojun Ji Jianchun Jiang Liming Lu Source Type: research

Recovery mechanism of heterotrophic ammonia assimilation system under chromium hexavalent stress
This study investigated the inhibitory effects and self-restoration mechanisms of Cr (VI) at various stress levels. The removal efficiencies of NH4+-N and Cr (VI) in the HAA gradually decreased with increasing influent Cr (VI) concentration. Exposure to Cr (VI) increased the amounts of high-molecular-weight proteins in soluble microbial products and stimulated the generation of extracellular polymeric substances. Heterotrophic functional microorganisms with Cr (VI) tolerance, such as Marinobacter and Planktosalinus, were enriched. An assimilation pathway gene (glnA) and a Cr (VI)-related gene (atoB) were also upregulated. ...
Source: Bioresource Technology - March 21, 2024 Category: Biotechnology Authors: Tong Jiao Chuanfu Zhao Mengru Zhang Fei Han Zhe Liu Shuhui Zhang Weizhi Zhou Source Type: research

Effects of culture temperature and light regimes on the biomass and lipid accumulation of Chlamydomonas reinhardtii under carbon-rich and nitrogen-limited conditions
This study investigated the impacts of various culture temperatures and light regimes on growth and biochemical constituents of Chlamydomonas reinhardtii under carbon-supply and nitrogen-limited conditions to improve oil production in algal cells. Results displayed that under a 30 ℃ and 150 μE/m2/s regime, there was a significant increase in biomass, total lipids, and lipid productivity. Specifically, these parameters reached 1.83 g/L, 36.25 %, and 130.73 mg/L/d, respectively. Remarkably, prolonging the photoperiod further enhanced the aforementioned three parameters, reaching peak levels of 1.92 g/L, 41.10 %, and 157.5...
Source: Bioresource Technology - March 21, 2024 Category: Biotechnology Authors: Shiyan Zheng Shourui Sun Shangyun Zou Jiamei Song Lan Hua Hui Chen Qiang Wang Source Type: research

Phosphate additives promote humic acid carbon and nitrogen skeleton formation by regulating precursors and composting bacterial communities
This study aimed to compare the effect of different phosphate additives including superphosphate (CP) and MP [Mg(OH)2 + H3PO4] on nitrogen conversion, humus fractions formation and bacterial community in food waste compost. The results showed the ratio of humic acid nitrogen in total nitrogen (HA-N/TN) in CP increased by 49 %. Ammonium nitrogen accumulation was increased by 75 % (CP) and 44 % (MP). Spectroscopic techniques proved that phosphate addition facilitated the formation of complex structures in HA. CP enhanced the dominance of Saccharomonospora, while Thermobifida and Bacillus were improved in MP. Structural equat...
Source: Bioresource Technology - March 21, 2024 Category: Biotechnology Authors: Jing Su Yabin Zhan Yuan Chang Su Chang Yan Luo Peizhen Chen Xingling Tao Yunfeng Chen Li Yang Ting Xu Yuhui Qiao Ji Li Yuquan Wei Source Type: research

Advanced nitrogen and phosphorus removal in pilot-scale anaerobic/aerobic/anoxic system for municipal wastewater in Northern China
This study has significant implications for the practical application of the AOA-SBR process.PMID:38513924 | DOI:10.1016/j.biortech.2024.130616 (Source: Bioresource Technology)
Source: Bioresource Technology - March 21, 2024 Category: Biotechnology Authors: Changkun Gu Xiyao Li Shujun Zhang Jianwei Li Xiaoyu Gao Guo Chen Zhibin Wang Yongzhen Peng Source Type: research

Exploiting synthetic biology platforms for enhanced biosynthesis of natural products in Yarrowia lipolytica
Bioresour Technol. 2024 Mar 19:130614. doi: 10.1016/j.biortech.2024.130614. Online ahead of print.ABSTRACTWith the rapid development of synthetic biology, researchers can design, modify, or even synthesize microorganisms de novo, and microorganisms endowed with unnatural functions can be considered "artificial life" and facilitate the development of functional products. Based on this concept, researchers can solve critical problems related to the insufficient supply of natural products, such as low yields, long production cycles, and cumbersome procedures. Due to its superior performance and unique physiological and bioche...
Source: Bioresource Technology - March 21, 2024 Category: Biotechnology Authors: Dahai Jiang Manqi Yang Kai Chen Wenxuan Jiang Liangliang Zhang Xiaojun Ji Jianchun Jiang Liming Lu Source Type: research

Recovery mechanism of heterotrophic ammonia assimilation system under chromium hexavalent stress
This study investigated the inhibitory effects and self-restoration mechanisms of Cr (VI) at various stress levels. The removal efficiencies of NH4+-N and Cr (VI) in the HAA gradually decreased with increasing influent Cr (VI) concentration. Exposure to Cr (VI) increased the amounts of high-molecular-weight proteins in soluble microbial products and stimulated the generation of extracellular polymeric substances. Heterotrophic functional microorganisms with Cr (VI) tolerance, such as Marinobacter and Planktosalinus, were enriched. An assimilation pathway gene (glnA) and a Cr (VI)-related gene (atoB) were also upregulated. ...
Source: Bioresource Technology - March 21, 2024 Category: Biotechnology Authors: Tong Jiao Chuanfu Zhao Mengru Zhang Fei Han Zhe Liu Shuhui Zhang Weizhi Zhou Source Type: research