Exploring effective light spectral conversion techniques for enhanced production of Spirulina-derived blue pigment protein, c-phycocyanin
Bioresour Technol. 2024 Mar 18:130612. doi: 10.1016/j.biortech.2024.130612. Online ahead of print.ABSTRACTSpirulina is a promising feedstock for c-phycocyanin, a blue pigment-protein, commercially incorporated in many food products for its desirable bright blue attributes, exceptional bioavailability, and inherent therapeutic properties. Remarkably, enhancing c-phycocyanin synthesis in Spirulina would facilitate economic viability and sustainability at large-scale production, as the forecasted market value is $ 409.8 million by 2030. Notably, the lighting source plays a key role in enhancing c-phycocyanin in Spirulina, and...
Source: Bioresource Technology - March 20, 2024 Category: Biotechnology Authors: Bavatharny Thevarajah Sajani Piyatilleke P H V Nimarshana S Koushalya Anushree Malik Thilini U Ariyadasa Source Type: research
One-pot sustainable synthesis of glucosylglycerate from starch and glycerol through artificial in vitro enzymatic cascade
Bioresour Technol. 2024 Mar 19;399:130611. doi: 10.1016/j.biortech.2024.130611. Online ahead of print.ABSTRACTGlucosylglycerate (R-2-O-α-D-glucopyranosyl-glycerate, GG) is a negatively charged compatible solution with versatile functions. Here, an artificial in vitro enzymatic cascade was designed to feasibly and sustainably produce GG from affordable starch and glycerol. First, Spirochaeta thermophila glucosylglycerate phosphorylase (GGP) was carefully selected because of its excellent heterologous expression, specific activity, and thermostability. The optimized two-enzyme cascade, consisting of alpha-glucan phosphoryla...
Source: Bioresource Technology - March 20, 2024 Category: Biotechnology Authors: Juanjuan Liu Mengfei Ren Haoran Ma Huilin Zhang Xinyu Cui Runyuan Kang Xinming Feng Dongdong Meng Source Type: research
Optimizing cultivation strategies and scaling up for fucoxanthin production using Pavlova sp
In this study, three brown marine microalgae, namely Skeletonema costatum, Chaetoceros gracilis, and Pavlova sp., were screened for fucoxanthin production. All strains displayed promising results, with Pavlova sp. exhibiting the highest fucoxanthin content (27.91 mg/g) and productivity (1.16 mg/L·day). Moreover, the influence of various cultivation parameters, such as culture media, salinity, sodium nitrate concentration, inoculum size, light intensity, and iron concentration, were investigated and optimized, resulting in a maximum fucoxanthin productivity of 7.89 mg/L·day. The investigation was further expanded to large...
Source: Bioresource Technology - March 20, 2024 Category: Biotechnology Authors: Chun-Yen Chen Ping-Yung Liu Yu-Han Chang Dillirani Nagarajan Mary Joy D Latagan Mark Daniel G de Luna Jih-Heng Chen Jo-Shu Chang Source Type: research
Extraction, recovery, and characterization of lignin from industrial corn stover lignin cake
Bioresour Technol. 2024 Mar 18:130610. doi: 10.1016/j.biortech.2024.130610. Online ahead of print.ABSTRACTLignin utilization in value-added co-products is an important component of enabling cellulosic biorefinery economics. However, aqueous dilute acid pretreatments yield lignins with limited applications due to significant modification during pretreatment, low solubility in many solvents, and high content of impurities (ash, insoluble polysaccharides). This work addresses these challenges and investigates the extraction and recovery of lignins from lignin-rich insoluble residue following dilute acid pretreatment and enzym...
Source: Bioresource Technology - March 20, 2024 Category: Biotechnology Authors: Vill ő Enikő Bécsy-Jakab Anthony Savoy Brian K Saulnier Sandip K Singh David B Hodge Source Type: research
Exploring effective light spectral conversion techniques for enhanced production of Spirulina-derived blue pigment protein, c-phycocyanin
Bioresour Technol. 2024 Mar 18:130612. doi: 10.1016/j.biortech.2024.130612. Online ahead of print.ABSTRACTSpirulina is a promising feedstock for c-phycocyanin, a blue pigment-protein, commercially incorporated in many food products for its desirable bright blue attributes, exceptional bioavailability, and inherent therapeutic properties. Remarkably, enhancing c-phycocyanin synthesis in Spirulina would facilitate economic viability and sustainability at large-scale production, as the forecasted market value is $ 409.8 million by 2030. Notably, the lighting source plays a key role in enhancing c-phycocyanin in Spirulina, and...
Source: Bioresource Technology - March 20, 2024 Category: Biotechnology Authors: Bavatharny Thevarajah Sajani Piyatilleke P H V Nimarshana S Koushalya Anushree Malik Thilini U Ariyadasa Source Type: research
One-pot sustainable synthesis of glucosylglycerate from starch and glycerol through artificial in vitro enzymatic cascade
Bioresour Technol. 2024 Mar 18:130611. doi: 10.1016/j.biortech.2024.130611. Online ahead of print.ABSTRACTGlucosylglycerate (R-2-O-α-D-glucopyranosyl-glycerate, GG) is a negatively charged compatible solution with versatile functions. Here, an artificial in vitro enzymatic cascade was designed to feasibly and sustainably produce GG from affordable starch and glycerol. First, Spirochaeta thermophila glucosylglycerate phosphorylase (GGP) was carefully selected because of its excellent heterologous expression, specific activity, and thermostability. The optimized two-enzyme cascade, consisting of alpha-glucan phosphorylase (...
Source: Bioresource Technology - March 20, 2024 Category: Biotechnology Authors: Juanjuan Liu Mengfei Ren Haoran Ma Huilin Zhang Xinyu Cui Runyuan Kang Xinming Feng Dongdong Meng Source Type: research
Optimizing cultivation strategies and scaling up for fucoxanthin production using Pavlova sp
In this study, three brown marine microalgae, namely Skeletonema costatum, Chaetoceros gracilis, and Pavlova sp., were screened for fucoxanthin production. All strains displayed promising results, with Pavlova sp. exhibiting the highest fucoxanthin content (27.91 mg/g) and productivity (1.16 mg/L·day). Moreover, the influence of various cultivation parameters, such as culture media, salinity, sodium nitrate concentration, inoculum size, light intensity, and iron concentration, were investigated and optimized, resulting in a maximum fucoxanthin productivity of 7.89 mg/L·day. The investigation was further expanded to large...
Source: Bioresource Technology - March 20, 2024 Category: Biotechnology Authors: Chun-Yen Chen Ping-Yung Liu Yu-Han Chang Dillirani Nagarajan Mary Joy D Latagan Mark Daniel G de Luna Jih-Heng Chen Jo-Shu Chang Source Type: research
Extraction, recovery, and characterization of lignin from industrial corn stover lignin cake
Bioresour Technol. 2024 Mar 18:130610. doi: 10.1016/j.biortech.2024.130610. Online ahead of print.ABSTRACTLignin utilization in value-added co-products is an important component of enabling cellulosic biorefinery economics. However, aqueous dilute acid pretreatments yield lignins with limited applications due to significant modification during pretreatment, low solubility in many solvents, and high content of impurities (ash, insoluble polysaccharides). This work addresses these challenges and investigates the extraction and recovery of lignins from lignin-rich insoluble residue following dilute acid pretreatment and enzym...
Source: Bioresource Technology - March 20, 2024 Category: Biotechnology Authors: Vill ő Enikő Bécsy-Jakab Anthony Savoy Brian K Saulnier Sandip K Singh David B Hodge Source Type: research
Potential directions for future development of mainstream partial nitrification-anammox processes: Ammonia-oxidizing archaea as novel functional microorganisms providing nitrite
Bioresour Technol. 2024 Mar 16;399:130605. doi: 10.1016/j.biortech.2024.130605. Online ahead of print.ABSTRACTThe application of ammonia-oxidizing archaea (AOA)-based partial nitrification-anammox (PN-A) for mainstream wastewater treatment has attracted research interest because AOA can maintain higher activity in low-temperature environments and they have higher affinity for oxygen and ammonia-nitrogen compared with ammonia-oxidizing bacteria (AOB), thus facilitating stabilized nitrite production, deep removal of low-ammonia, and nitrite-oxidizing bacteria suppression. Moreover, the low affinity of AOA for ammonia makes t...
Source: Bioresource Technology - March 18, 2024 Category: Biotechnology Authors: Kehuan Guo Dong Li Tongyao Hao Luyao Teng Shuai Li Huiping Zeng Jie Zhang Source Type: research
Effects of hematite on two types of dissolved organic compounds in lignocellulosic anaerobic hydrolysate: Lignin-derived aromatic compounds and denitrifying carbon sources
This study aims to investigate the influence of hematite on lignin-derived aromatic compounds and denitrifying carbon sources, as well as to identify iron-reducing bacteria that utilize lignin-derived aromatic compounds as electron donors. The findings revealed that hematite facilitated the anaerobic fermentation of plant biomass, resulting in the production of small molecular organic acids. Moreover, biodegradation of lignin-derived aromatic compounds led to the formation of phenolic acids, while an increased generation of denitrifying carbon sources enhanced nitrogen removal efficiency by 13.84 %. Additionally, due to ad...
Source: Bioresource Technology - March 18, 2024 Category: Biotechnology Authors: Xinjing Li Xiangwei Huang Xingbao Hu Yunxiao Chong Source Type: research
Novel manganese and nitrogen co-doped biochar based on sodium bicarbonate activation for efficient removal of bisphenol A: Mechanism insight and role analysis of manganese and nitrogen by combination of characterizations, experiments and density functional theory calculations
Bioresour Technol. 2024 Mar 17;399:130608. doi: 10.1016/j.biortech.2024.130608. Online ahead of print.ABSTRACTA novel porous manganese and nitrogen co-doped biochar (Mn-N@SBC) was synthesized via one-step pyrolysis, utilizing loofah agricultural waste as the precursor and NaHCO3 as the activator. The behavior of bisphenol A adsorbed on Mn-N@SBC was evaluated using static batch adsorption experiments. Compared to direct manganese-nitrogen co-doping, co-doping based on NaHCO3 activation significantly increased the specific surface area (231 to 1027 m2·g-1) and adsorption capacity (15 to 351 mg·g-1). Wide pH (2-10) and good...
Source: Bioresource Technology - March 18, 2024 Category: Biotechnology Authors: Wenlong Wu Jinwei Zhang Weijie Zhu Shouhui Zhao Yuchen Gao Yan Li Lei Ding Heng Ding Source Type: research
Unlocking synergies: Harnessing the potential of biological methane sequestration through metabolic coupling between Methylomicrobium alcaliphilum 20Z and Chlorella sp. HS2
Bioresour Technol. 2024 Mar 16:130607. doi: 10.1016/j.biortech.2024.130607. Online ahead of print.ABSTRACTA halotolerant consortium between microalgae and methanotrophic bacteria could effectively remediate in situ CH4 and CO2, particularly using saline wastewater sources. Herein, Methylomicrobium alcaliphilum 20Z was demonstrated to form a mutualistic association with Chlorella sp. HS2 at a salinity level above 3.0%. Co-culture significantly enhanced the growth of both microbes, independent of initial inoculum ratios. Additionally, increased methane provision in enclosed serum bottles led to saturated methane removal. Sub...
Source: Bioresource Technology - March 18, 2024 Category: Biotechnology Authors: Jin-Ho Yun Hyewon Lee Jang-Won Nam Minji Ko Jaehyun Park Dae-Hee Lee Seung-Goo Lee Hee-Sik Kim Source Type: research
Formation/characterization of humin-mediated anaerobic granular sludge and enhanced methanogenic performance
This study presents a novel method for accelerating the granulation of methanogenic anaerobic granular sludge (AnGS) in an upflow anaerobic sludge blanket (UASB) reactor using solid-phase humin (HM). The results demonstrated that HM-mediated AnGS (HM-AnGS) formed rapidly within 50 days. The increase in particle size, settling velocity and mechanical strength was attributed to the rapid granulation of the HM-AnGS. The maximum methane yield of the HM-AnGS was 5-fold higher than that of the control group. This is consistent with the findings, which showed that HM-AnGS had 3.2-3.4 times more methyl-coenzyme M reductase (Mcr) a...
Source: Bioresource Technology - March 18, 2024 Category: Biotechnology Authors: Ningli Zhou Zhixing Xiao Dan Chen Source Type: research
Insight into performance of nitrogen removal enhanced by adding lactic acid-rich food waste fermentation liquid as carbon source in municipal wastewater treatment
Bioresour Technol. 2024 Mar 16:130602. doi: 10.1016/j.biortech.2024.130602. Online ahead of print.ABSTRACTLactic acid-rich fermentation liquid (LAFL) of food waste is found to act as a promising alternative carbon source for nitrogen removal in wastewater treatment. Here, LAFL was employed to investigate its impacts on nitrogen removal during raw municipal wastewater treatment with a comparison to sodium acetate (NaAc). Results indicated that nitrogen removals were comparable when incorporated with LAFL and NaAc (92.89 % v.s. 91.23 %). Unlike the utilization of NaAc, using LAFL could avoid suppressing the relative abundanc...
Source: Bioresource Technology - March 18, 2024 Category: Biotechnology Authors: Mingjiang Zhang Guangliang Zhao Xinxin Wang Bo Zhou Yujun Zhou Dianzhan Wang Jianru Liang Lixiang Zhou Source Type: research
Coupling of gene regulation and carrier modification manipulates bacterial biofilms as robust living catalysts
Bioresour Technol. 2024 Mar 16:130604. doi: 10.1016/j.biortech.2024.130604. Online ahead of print.ABSTRACTThe biofilm of an engineered strain is limited by slow growth and low yield, resulting in an unsatisfactory ability to resist external stress and promote catalytic efficiency. Here, biofilms used as robust living catalysts were manipulated through dual functionalized gene regulation and carrier modification strategy. The results showed that gene overexpression regulates the autoinducer-2 activity, extracellular polymeric substance content and colony behavior of Escherichia coli, and the biofilm yield of csgD overexpres...
Source: Bioresource Technology - March 18, 2024 Category: Biotechnology Authors: Cheng-Hai Yan Yu-Fan Zhan Huan Chen Richard A Herman Yan Xu Marriam Khurshid Lu-Chan Gong Shuai You Jun Wang Source Type: research
