Enhancing simultaneous saccharification and co-fermentation of corncob by Kluyveromyces marxianus through overexpression of putative transcription regulator
Bioresour Technol. 2024 Mar 24;399:130627. doi: 10.1016/j.biortech.2024.130627. Online ahead of print.ABSTRACTOverexpression of a gene with unknown function in Kluyveromyces marxianus markedly improved tolerance to lignocellulosic biomass-derived inhibitors. This overexpression also enhanced tolerance to elevated temperatures, ethanol, and high concentrations of NaCl and glucose. Inhibitor degradation and transcriptome analyses related this K. marxianusMultiple Stress Resistance (KmMSR) gene to the robustness of yeast cells. Nuclear localization and DNA-binding domain analyses indicate that KmMsr is a putative transcriptio...
Source: Bioresource Technology - March 24, 2024 Category: Biotechnology Authors: Kehui Zhang Ziyun Jiang Xingjiang Li Dongmei Wang Jiong Hong Source Type: research
Dual disintegration of microalgae biomass for cost-effective biomethane production: Energy and cost assessment
Bioresour Technol. 2024 Mar 24;399:130630. doi: 10.1016/j.biortech.2024.130630. Online ahead of print.ABSTRACTThe present study aims to enhance the biomethane production potential of microalgae via a dual disintegration process. During this process, the microalgae biomass was firstly subjected to cell wall weakening by thermochemical disintegration (TC) (50 to 80 °C), pH adjustment with alkali, NaOH (6 to 10) and time (0 to 10 min) and, secondly, by bacterial disintegration (BD). TC-BD disintegration was comparatively higher (33 %) than BD (24 %), TC (8.5 %), and control (7 %). A more significant VFA accumulation of 2816 ...
Source: Bioresource Technology - March 24, 2024 Category: Biotechnology Authors: Yukesh Kannah Ravi S Kavitha Siham Y Al-Qaradawi J Rajesh Banu Source Type: research
Enhancing simultaneous saccharification and co-fermentation of corncob by Kluyveromyces marxianus through overexpression of putative transcription regulator
Bioresour Technol. 2024 Mar 24;399:130627. doi: 10.1016/j.biortech.2024.130627. Online ahead of print.ABSTRACTOverexpression of a gene with unknown function in Kluyveromyces marxianus markedly improved tolerance to lignocellulosic biomass-derived inhibitors. This overexpression also enhanced tolerance to elevated temperatures, ethanol, and high concentrations of NaCl and glucose. Inhibitor degradation and transcriptome analyses related this K. marxianusMultiple Stress Resistance (KmMSR) gene to the robustness of yeast cells. Nuclear localization and DNA-binding domain analyses indicate that KmMsr is a putative transcriptio...
Source: Bioresource Technology - March 24, 2024 Category: Biotechnology Authors: Kehui Zhang Ziyun Jiang Xingjiang Li Dongmei Wang Jiong Hong Source Type: research
Dual disintegration of microalgae biomass for cost-effective biomethane production: Energy and cost assessment
Bioresour Technol. 2024 Mar 22:130630. doi: 10.1016/j.biortech.2024.130630. Online ahead of print.ABSTRACTThe present study aims to enhance the biomethane production potential of microalgae via a dual disintegration process. During this process, the microalgae biomass was firstly subjected to cell wall weakening by thermochemical disintegration (TC) (50 to 80 °C), pH adjustment with alkali, NaOH (6 to 10) and time (0 to 10 min) and, secondly, by bacterial disintegration (BD). TC-BD disintegration was comparatively higher (33 %) than BD (24 %), TC (8.5 %), and control (7 %). A more significant VFA accumulation of 2816 mg/L...
Source: Bioresource Technology - March 24, 2024 Category: Biotechnology Authors: Yukesh Kannah Ravi S Kavitha Siham Y Al-Qaradawi J Rajesh Banu Source Type: research
Enhancing simultaneous saccharification and co-fermentation of corncob by Kluyveromyces marxianus through overexpression of putative transcription regulator
Bioresour Technol. 2024 Mar 22:130627. doi: 10.1016/j.biortech.2024.130627. Online ahead of print.ABSTRACTOverexpression of a gene with unknown function in Kluyveromyces marxianus markedly improved tolerance to lignocellulosic biomass-derived inhibitors. This overexpression also enhanced tolerance to elevated temperatures, ethanol, and high concentrations of NaCl and glucose. Inhibitor degradation and transcriptome analyses related this K. marxianusMultiple Stress Resistance (KmMSR) gene to the robustness of yeast cells. Nuclear localization and DNA-binding domain analyses indicate that KmMsr is a putative transcriptional ...
Source: Bioresource Technology - March 24, 2024 Category: Biotechnology Authors: Kehui Zhang Ziyun Jiang Xingjiang Li Dongmei Wang Jiong Hong Source Type: research
Dual disintegration of microalgae biomass for cost-effective biomethane production: Energy and cost assessment
Bioresour Technol. 2024 Mar 22:130630. doi: 10.1016/j.biortech.2024.130630. Online ahead of print.ABSTRACTThe present study aims to enhance the biomethane production potential of microalgae via a dual disintegration process. During this process, the microalgae biomass was firstly subjected to cell wall weakening by thermochemical disintegration (TC) (50 to 80 °C), pH adjustment with alkali, NaOH (6 to 10) and time (0 to 10 min) and, secondly, by bacterial disintegration (BD). TC-BD disintegration was comparatively higher (33 %) than BD (24 %), TC (8.5 %), and control (7 %). A more significant VFA accumulation of 2816 mg/L...
Source: Bioresource Technology - March 24, 2024 Category: Biotechnology Authors: Yukesh Kannah Ravi S Kavitha Siham Y Al-Qaradawi J Rajesh Banu Source Type: research
Machine learning application for predicting key properties of activated carbon produced from lignocellulosic biomass waste with chemical activation
Bioresour Technol. 2024 Mar 21:130624. doi: 10.1016/j.biortech.2024.130624. Online ahead of print.ABSTRACTThe successful application of gradient boosting regression (GBR) in machine learning to forecast surface area, pore volume, and yield in biomass-derived activated carbon (AC) production underscores its potential for enhancing manufacturing processes. The GBR model, collecting 17 independent variables for two-step activation (2-SA) and 14 for one-step activation (1-SA), demonstrates effectiveness across three datasets-1-SA, 2-SA, and a combined dataset. Notably, in 1-SA, the GBR model yields R2 values of 0.76, 0.90, and...
Source: Bioresource Technology - March 23, 2024 Category: Biotechnology Authors: Rongge Zou Zhibin Yang Jiahui Zhang Ryan Lei William Zhang Fitria Fnu Daniel C W Tsang Joshua Heyne Xiao Zhang Roger Ruan Hanwu Lei Source Type: research
Productivity, amino acid profile, and protein bioaccessibility in heterotrophic batch cultivation of Galdieria sulphuraria
Bioresour Technol. 2024 Mar 21;399:130628. doi: 10.1016/j.biortech.2024.130628. Online ahead of print.ABSTRACTThe polyextremophilic Galdieria sulphuraria is emerging as a promising microalgal species for food applications. This work explores the potential of heterotrophically cultivated G. sulphuraria as a protein producer for human consumption. To this end, the performances of four G. sulphuraria strains grown under the same conditions were compared. Amino acid profiles varied among strains and growth phases, but all samples met FAO dietary requirements for adults. The specific growth rates were between 1.01 and 1.48 day-...
Source: Bioresource Technology - March 23, 2024 Category: Biotechnology Authors: F Abiusi B Tumulero L Neutsch A Mathys Source Type: research
Response of bacterial and micro-eukaryotic communities to spatio-temporal fluctuations of wastewater in full scale constructed wetlands
Bioresour Technol. 2024 Mar 22;399:130626. doi: 10.1016/j.biortech.2024.130626. Online ahead of print.ABSTRACTHow microbial communities respond to wastewater fluctuations is poorly understood. Full-scale surface flow constructed wetlands (SFCWs) were constructed for investigating microbial communities. Results showed that influent wastewater changed sediment bacterial community composition seasonally, indicating that a single bacterial taxonomic group had low resistance (especially, Actinobacteriota and Gammaproteobacteria). However, copy numbers of 16S rRNA, ammonia oxidizing archaea, ammonia oxidizing bacteria, nirS and ...
Source: Bioresource Technology - March 23, 2024 Category: Biotechnology Authors: Xi Li Menghua Xia Lemian Liu Yuyuan Li Jinshui Wu Source Type: research
Machine learning application for predicting key properties of activated carbon produced from lignocellulosic biomass waste with chemical activation
Bioresour Technol. 2024 Mar 21:130624. doi: 10.1016/j.biortech.2024.130624. Online ahead of print.ABSTRACTThe successful application of gradient boosting regression (GBR) in machine learning to forecast surface area, pore volume, and yield in biomass-derived activated carbon (AC) production underscores its potential for enhancing manufacturing processes. The GBR model, collecting 17 independent variables for two-step activation (2-SA) and 14 for one-step activation (1-SA), demonstrates effectiveness across three datasets-1-SA, 2-SA, and a combined dataset. Notably, in 1-SA, the GBR model yields R2 values of 0.76, 0.90, and...
Source: Bioresource Technology - March 23, 2024 Category: Biotechnology Authors: Rongge Zou Zhibin Yang Jiahui Zhang Ryan Lei William Zhang Fitria Fnu Daniel C W Tsang Joshua Heyne Xiao Zhang Roger Ruan Hanwu Lei Source Type: research
Productivity, amino acid profile, and protein bioaccessibility in heterotrophic batch cultivation of Galdieria sulphuraria
Bioresour Technol. 2024 Mar 21:130628. doi: 10.1016/j.biortech.2024.130628. Online ahead of print.ABSTRACTThe polyextremophilic Galdieria sulphuraria is emerging as a promising microalgal species for food applications. This work explores the potential of heterotrophically cultivated G. sulphuraria as a protein producer for human consumption. To this end, the performances of four G. sulphuraria strains grown under the same conditions were compared. Amino acid profiles varied among strains and growth phases, but all samples met FAO dietary requirements for adults. The specific growth rates were between 1.01 and 1.48 day-1. A...
Source: Bioresource Technology - March 23, 2024 Category: Biotechnology Authors: F Abiusi B Tumulero L Neutsch A Mathys Source Type: research
Response of bacterial and micro-eukaryotic communities to spatio-temporal fluctuations of wastewater in full scale constructed wetlands
Bioresour Technol. 2024 Mar 21:130626. doi: 10.1016/j.biortech.2024.130626. Online ahead of print.ABSTRACTHow microbial communities respond to wastewater fluctuations is poorly understood. Full-scale surface flow constructed wetlands (SFCWs) were constructed for investigating microbial communities. Results showed that influent wastewater changed sediment bacterial community composition seasonally, indicating that a single bacterial taxonomic group had low resistance (especially, Actinobacteriota and Gammaproteobacteria). However, copy numbers of 16S rRNA, ammonia oxidizing archaea, ammonia oxidizing bacteria, nirS and nirK...
Source: Bioresource Technology - March 23, 2024 Category: Biotechnology Authors: Xi Li Menghua Xia Lemian Liu Yuyuan Li Jinshui Wu Source Type: research
Efficiency of aqueous oleosome extraction from capsicum seeds compared to classical oil extraction
This study assesses the latter using an exergy assessment of oleosome extraction for food emulsions. The contribution of each part of the process to the overall impact was investigated. Based on current lab-scale data, oleosome extraction has nearly twice the exergy loss compared to the industrial process of oil extraction and industrial assembly of emulsions. The exergy losses of the lab-scale oleosome extraction are currently dominated by the chemical exergy associated with product loss during the separation of oleosomes from the rest of the biomass. This loss is expected to significantly decrease when upscaled to indust...
Source: Bioresource Technology - March 22, 2024 Category: Biotechnology Authors: Mingzhao Han Stefan Ten Voorde Xin Wen Yuanying Ni Remko M Boom Constantinos V Nikiforidis Source Type: research
Microbial mechanisms involved in negative effects of amoxicillin and copper on humification during composting of dairy cattle manure
Bioresour Technol. 2024 Mar 20:130623. doi: 10.1016/j.biortech.2024.130623. Online ahead of print.ABSTRACTLivestock manure often contains various pollutants. The aim of this study was to investigate how adding amoxicillin (AMX), Cu, and both AMX and Cu (ACu) affected humification during composting and the microbial mechanisms involved. The cellulose degradation rates were 16.96%, 10.86%, and 9.01% lower, the humic acid contents were 18.71%, 12.89%, and 16.78% lower, and the humification degrees were 24.72%, 24.16%, and 15.73% lower for the AMX, Cu, and ACu treatments, respectively, than the control. Adding AMX and Cu separ...
Source: Bioresource Technology - March 22, 2024 Category: Biotechnology Authors: Ya Su Ling Zhou Qianting Zhuo Chen Fang Jia You Lujia Han Guangqun Huang Source Type: research
Harvesting marine microalgae Tetraselmis sp. Using cellulose acetate membrane
This study presents the development and application of a cellulose acetate phase-inversion membrane for the efficient harvesting of Tetraselmis sp., a promising alternative for aquaculture feedstock. Once fabricated, the cellulose acetate membrane was characterized, and its performance was evaluated through the filtration of Tetraselmis sp. broth. The results demonstrated that the developed membrane exhibited exceptional microalgae harvesting efficiency. It showed a low intrinsic resistance and a high clean water permeability of 1100 L/(m2·h·bar), enabling high-throughput filtration of Tetraselmis sp. culture with a perm...
Source: Bioresource Technology - March 22, 2024 Category: Biotechnology Authors: Afrillia Fahrina Fahrurrozi Fahrurrozi Hendra Munandar Varian Fahmi Sutarat Thongratkaew Kajornsak Faungnawakij Muhammad Roil Bilad Source Type: research
