Life cycle assessment and techno-economic analysis of wood-based biorefineries for cellulosic ethanol production
Bioresour Technol. 2024 Mar 15:130595. doi: 10.1016/j.biortech.2024.130595. Online ahead of print.ABSTRACTPoplar is widely used in the paper industry and accompanied by abundant branches waste, which is potential feedstock for bioethanol production. Acid-chlorite pretreatment can selectively remove lignin, thereby significantly increasing enzymatic efficiency. Moreover, lignin residues valorization via gasification-syngas fermentation can achieve higher fuel yield. Herein, environmental and economic aspects were conducted to assess technological routes, which guides further process optimization. Life cycle assessment resul...
Source: Bioresource Technology - March 17, 2024 Category: Biotechnology Authors: Qian Qian Zhongyang Luo Haoran Sun Qi Wei Jingkang Shi Longfei Li Source Type: research

Enhancing wastewater treatment efficiency: A hybrid technology perspective with energy-saving strategies
Bioresour Technol. 2024 Mar 15:130593. doi: 10.1016/j.biortech.2024.130593. Online ahead of print.ABSTRACTThe study aimed to investigate how hybrid technology, combined with various intermittent aeration (IA) strategies, contributes to reducing the energy costs of wastewater treatment while simultaneously ensuring a high treatment efficiency. Even with IA subphases lasting half as long as those without aeration, and oxygen levels reduced from 3.5 to 1.5 mg O2/L, pollutants removal efficiency remains robust, allowing for a 1.41-fold reduction in energy consumption (EO). Hybrid technology led to a 1.34-fold decrease in EO, a...
Source: Bioresource Technology - March 17, 2024 Category: Biotechnology Authors: Olga Zajac Magdalena Zielinska Monika Zubrowska-Sudol Source Type: research

Enhanced triolein and ethyl ferulate interesterification performance by CRL-AuNPs
This study established a Candida rugosa lipase (CRL) system to catalyze triolein and ethyl ferulate interesterification. The products were identified, and the binding mode between the substrates and CRL was predicted through molecular docking. Three methods for preparing CRL-AuNPs were proposed and characterized. It was found that the addition of 40 mL of 15 nm gold nanoparticles increased the CRL activity from 3.05 U/mg to 4.75 U/mg, but the hybridization efficiency was only 32.7 %. By using 4 mL of 0.1 mg/mL chloroauric acid, the hybridization efficiency was improved to 50.7 %, but the enzyme activity was sharply decreas...
Source: Bioresource Technology - March 17, 2024 Category: Biotechnology Authors: Zhang Kai Xin Jiaying Lu Xuechun Source Type: research

Biosynthetic pathway redesign in non-conventional yeast for enhanced production of cembratriene-ol
Bioresour Technol. 2024 Mar 15:130596. doi: 10.1016/j.biortech.2024.130596. Online ahead of print.ABSTRACTCembratriene-ol (CBT-ol), a plant-derived macrocyclic diterpene with notable insecticidal activity, has attracted considerable attention with respect to the development of sustainable and green biopesticides. Currently, CBT-ol production is limited by an inefficient and costly plant extraction strategy. Herein, CBT-ol production was enhanced by redesigning the CBT-ol biosynthetic pathway in Candida tropicalis, with subsequent truncation of CBT-ol synthase further increasing CBT-ol production. Moreover, bottlenecks in t...
Source: Bioresource Technology - March 17, 2024 Category: Biotechnology Authors: Lihua Zhang Cheng Fan Haiquan Yang Yuanyuan Xia Wei Shen Xianzhong Chen Source Type: research

Valorization of Nannochloropsis oceanica for integrated co-production of violaxanthin cycle carotenoids
In this study, the economical microalga Nannochloropsis oceanica was identified as an accumulator of violaxanthin cycle carotenoids, including violaxanthin, antheraxanthin, and zeaxanthin. Notably, a novel and competent approach for the integrated co-production of violaxanthin cycle carotenoids was explored, encompassing four steps: microalgal cultivation, solvent extraction, ODS open-column chromatography, and ethanol precipitation. Under optimal co-production conditions, the purities of the obtained violaxanthin, antheraxanthin, and zeaxanthin all exceeded 92%, with total recovery rates of approximately 51%, 40%, and 60%...
Source: Bioresource Technology - March 17, 2024 Category: Biotechnology Authors: Rui Yin GengJie Zhuang Yuhui Lei Jichang Han Yanrong Li Jinrong Zhang Xiaojun Yan Source Type: research

Scale-up of microbial lipid and bioethanol production from oilcane
This study describes the processing of oilcane stems into juice and hydrothermally pretreated lignocellulosic hydrolysate and their valorization to ethanol and microbial oil using Saccharomyces cerevisiae and engineered Rhodosporidium toruloides strains, respectively. A bioethanol titer of 106 g/L was obtained from S. cerevisiae grown on oilcane juice in a 3 L fermenter, and a lipid titer of 8.8 g/L was obtained from R. toruloides grown on oilcane hydrolysate in a 75 L fermenter. Oil was extracted from the R. toruloides cells using supercritical CO2, and the observed fatty acid profile was consistent with previous studies ...
Source: Bioresource Technology - March 17, 2024 Category: Biotechnology Authors: Narendra Naik Deshavath William Woodruff Fred Eller Vionna Susanto Cindy Yang Christopher V Rao Vijay Singh Source Type: research

Energy and economic analysis of alternatives for the valorization of hydrogen rich stream produced in the aqueous phase reforming of pyrolysis bio-oil aqueous fraction
Bioresour Technol. 2024 Mar 14;399:130572. doi: 10.1016/j.biortech.2024.130572. Online ahead of print.ABSTRACTAqueous phase reforming has been explored for renewable H2 production from waste biomass. Promising results have been reported for pyrolysis bio-oil aqueous fractions (AFB), but economical assessments are needed to determine process feasibility, which requires both energy consumption minimization and optimal H2 valorization. This work compares different alternatives using process simulation and economic evaluation computational tools. Experimental results and a specific thermodynamic model are used to set mass bala...
Source: Bioresource Technology - March 16, 2024 Category: Biotechnology Authors: Francisco Heras J éssica Justicia Jos é A Baeza Miguel A Gilarranz V íctor R Ferro Source Type: research

Effect of hydrothermal carbonization on pyrolysis behavior, nutrients and metal species distribution in municipal sludge
In this study, the effect of hydrothermal carbonation (HTC) on the pyrolysis behavior and the distribution of nutrients and metal species of waste-activated sludge (WAS) was investigated. Results showed that the pyrolysis activation energy range of WAS decreased from 11-57 kJ/mol to 10-36 kJ/mol when the hydrothermal carbonization was at 160 °C. As indicated by thermodynamic parameters, the hydrothermal carbonization process reduces the pyrolysis reaction activity of the hydrochar. The results of the chemical analysis indicate that hydrothermal carbonization significantly enhances the release of phosphorus and nitrogen, w...
Source: Bioresource Technology - March 16, 2024 Category: Biotechnology Authors: Changhao Liu Zhengbo Yue Ding Ma Keyu Li Zhilin Xie Tianqin Zhang Jin Wang Source Type: research

Addressing raw material variability: in-line FTIR sugar composition analysis of lignocellulosic process streams
Bioresour Technol. 2024 Mar 14:130535. doi: 10.1016/j.biortech.2024.130535. Online ahead of print.ABSTRACTFor a sustainable economy, biorefineries that use second-generation feedstocks to produce biochemicals and biofuels are essential. However, the exact composition of renewable feedstocks depends on the natural raw materials used and is therefore highly variable. In this contribution, a process analytical technique (PAT) strategy for determining the sugar composition of lignocellulosic process streams in real-time to enable better control of bioprocesses is presented. An in-line mid-IR probe was used to acquire spectra o...
Source: Bioresource Technology - March 16, 2024 Category: Biotechnology Authors: Daniel Waldschitz Yannick Bus Christoph Herwig Julian Kager Oliver Spadiut Source Type: research

Addressing raw material variability: in-line FTIR sugar composition analysis of lignocellulosic process streams
Bioresour Technol. 2024 Mar 14:130535. doi: 10.1016/j.biortech.2024.130535. Online ahead of print.ABSTRACTFor a sustainable economy, biorefineries that use second-generation feedstocks to produce biochemicals and biofuels are essential. However, the exact composition of renewable feedstocks depends on the natural raw materials used and is therefore highly variable. In this contribution, a process analytical technique (PAT) strategy for determining the sugar composition of lignocellulosic process streams in real-time to enable better control of bioprocesses is presented. An in-line mid-IR probe was used to acquire spectra o...
Source: Bioresource Technology - March 16, 2024 Category: Biotechnology Authors: Daniel Waldschitz Yannick Bus Christoph Herwig Julian Kager Oliver Spadiut Source Type: research

Energy and economic analysis of alternatives for the valorization of hydrogen rich stream produced in the aqueous phase reforming of pyrolysis bio-oil aqueous fraction
Bioresour Technol. 2024 Mar 14:130572. doi: 10.1016/j.biortech.2024.130572. Online ahead of print.ABSTRACTAqueous phase reforming has been explored for renewable H2 production from waste biomass. Promising results have been reported for pyrolysis bio-oil aqueous fractions (AFB), but economical assessments are needed to determine process feasibility, which requires both energy consumption minimization and optimal H2 valorization. This work compares different alternatives using process simulation and economic evaluation computational tools. Experimental results and a specific thermodynamic model are used to set mass balances...
Source: Bioresource Technology - March 16, 2024 Category: Biotechnology Authors: Francisco Heras J éssica Justicia Jos é A Baeza Miguel A Gilarranz V íctor R Ferro Source Type: research

Effect of hydrothermal carbonization on pyrolysis behavior, nutrients and metal species distribution in municipal sludge
In this study, the effect of hydrothermal carbonation (HTC) on the pyrolysis behavior and the distribution of nutrients and metal species of waste-activated sludge (WAS) was investigated. Results showed that the pyrolysis activation energy range of WAS decreased from 11-57 kJ/mol to 10-36 kJ/mol when the hydrothermal carbonization was at 160 °C. As indicated by thermodynamic parameters, the hydrothermal carbonization process reduces the pyrolysis reaction activity of the hydrochar. The results of the chemical analysis indicate that hydrothermal carbonization significantly enhances the release of phosphorus and nitrogen, w...
Source: Bioresource Technology - March 16, 2024 Category: Biotechnology Authors: Changhao Liu Zhengbo Yue Ding Ma Keyu Li Zhilin Xie Tianqin Zhang Jin Wang Source Type: research

Corrigendum to "Membrane fouling mechanism of biofilm-membrane bioreactor (BF-MBR): Pore blocking model and membrane cleaning" [Bioresour. Technol. 250 (2018) 398-405]
Bioresour Technol. 2024 Mar 14;399:130564. doi: 10.1016/j.biortech.2024.130564. Online ahead of print.NO ABSTRACTPMID:38490139 | DOI:10.1016/j.biortech.2024.130564 (Source: Bioresource Technology)
Source: Bioresource Technology - March 15, 2024 Category: Biotechnology Authors: Yi Zheng Wenxiang Zhang Bing Tang Liying Bin Jie Ding Yi Zheng Zhien Zhang Source Type: research

Efficient production of R-2-(4-hydroxyphenoxy) propionic acid by Beauveria bassiana using biofilm-based two-stage fermentation
Bioresour Technol. 2024 Mar 13:130588. doi: 10.1016/j.biortech.2024.130588. Online ahead of print.ABSTRACTIn this work, a novel biofilm-based fermentation of Beauveria bassiana was employed to convert R-2- phenoxypropionic acid (R-PPA) to R-2-(4-hydroxyphenoxy) propionic acid (R-HPPA). The biofilm culture model of Beauveria bassiana produced a significantly higher R-HPPA titer than the traditional submerged fermentation method. Mannitol dosage, tryptone dosage, and initial pH were the factors that played a significant role in biofilm formation and R-HPPA synthesis. Under the optimal conditions, the maximum R-HPPA titer and...
Source: Bioresource Technology - March 15, 2024 Category: Biotechnology Authors: Shuping Zou Yizhi Ma Wei Ding Yongkang Jiang Xiaomin Chen Juan Chen Hailing Gao Yaping Xue Yuguo Zheng Source Type: research

Bio-sorption capacity of cadmium and zinc by Pseudomonas monteilii with heavy-metal resistance isolated from the compost of pig manure
Bioresour Technol. 2024 Mar 13:130589. doi: 10.1016/j.biortech.2024.130589. Online ahead of print.ABSTRACTThe tolerance of Pseudomonas monteilii X1, isolated from pig manure compost, to Cd and Zn, as well as its capacity for biosorption were investigated. The minimum inhibitory concentration (MIC) of Cd and Zn for the strain were 550 mg/L and 800 mg/L, respectively. Untargeted metabolomics analysis revealed that organic acids and derivatives, lipids and lipids-like molecules, and organic heterocyclic compounds were main metabolomics. The glyoxylate and dicarboxylate metabolism pathway were significantly enriched under Cd2+...
Source: Bioresource Technology - March 15, 2024 Category: Biotechnology Authors: Ping Wang Feixue Yue Cong Shao Qian Li Yining Shen Kaili Xu Zihan Shi Naiyu Liu Ronghua Li Zengqiang Zhang Source Type: research