Role of TGF β1 and WNT6 in FGF2 and BMP4-driven endothelial differentiation of murine embryonic stem cells
AbstractEmbryonic stem cells (ES) are a valuable source of endothelial cells. By co-culturing ES cells with the stromal PA6 cells, the endothelial commitment can be achieved by adding exogenous FGF2 or BMP4. In this work, the molecular pathways that direct the differentiation of ES cells toward endothelium in response to FGF2 are evaluated and compared to those activated by BMP4. To this purpose the genes expression profiles of both ES/PA6 co-cultures and of pure cultures of PA6 cells were obtained by microarray technique at different time points. The bioinformatics processing of the data indicated TGF β1 as the most repr...
Source: Angiogenesis - September 3, 2021 Category: Molecular Biology Source Type: research
PFKFB3 gene deletion in endothelial cells inhibits intraplaque angiogenesis and lesion formation in a murine model of venous bypass grafting
This study demonstrates that inhibition of endothelial glycolysis is a promising therapeutic strategy to slow down plaque progression. (Source: Angiogenesis)
Source: Angiogenesis - August 25, 2021 Category: Molecular Biology Source Type: research
Extracellular matrix stiffness controls VEGF165 secretion and neuroblastoma angiogenesis via the YAP/RUNX2/SRSF1 axis
(Source: Angiogenesis)
Source: Angiogenesis - August 18, 2021 Category: Molecular Biology Source Type: research
Refractory systemic capillary leak syndrome treated with bevacizumab: a case report
This article was about a 58-year-old female suffering from refractory systemic capillary leak syndrome after bone marrow transplantation and being treated with bevacizumab, a VEGF inhibitor. In comparison with other successfully treated cases, this patient received four cycles of bevacizumab treatment without symptomatic improvement and eventually died in the intensive care unit. Further studies are needed to further confirm the role of bevacizumab in the management of SCLS. (Source: Angiogenesis)
Source: Angiogenesis - August 14, 2021 Category: Molecular Biology Source Type: research
Inhibition of the prolyl isomerase Pin1 improves endothelial function and attenuates vascular remodelling in pulmonary hypertension by inhibiting TGF- β signalling
AbstractPulmonary arterial hypertension (PAH) is a devastating disease, characterized by obstructive pulmonary vascular remodelling ultimately leading to right ventricular (RV) failure and death. Disturbed transforming growth factor- β (TGF-β)/bone morphogenetic protein (BMP) signalling, endothelial cell dysfunction, increased proliferation of smooth muscle cells and fibroblasts, and inflammation contribute to this abnormal remodelling. Peptidyl-prolyl isomerase Pin1 has been identified as a critical driver of proliferation an d inflammation in vascular cells, but its role in the disturbed TGF-β/BMP signalling, endothel...
Source: Angiogenesis - August 11, 2021 Category: Molecular Biology Source Type: research
Correction to: Depletion of Arg/Abl2 improves endothelial cell adhesion and prevents vascular leak during inflammation
(Source: Angiogenesis)
Source: Angiogenesis - August 9, 2021 Category: Molecular Biology Source Type: research
CCM2-deficient endothelial cells undergo a ROCK-dependent reprogramming into senescence-associated secretory phenotype
AbstractCerebral cavernous malformation (CCM) is a cerebrovascular disease in which stacks of dilated haemorrhagic capillaries form focally in the brain. Whether and how defective mechanotransduction, cellular mosaicism and inflammation interplay to sustain the progression of CCM disease is unknown. Here, we reveal that CCM1- and CCM2-silenced endothelial cells expanded in vitro enter into senescence-associated secretory phenotype (SASP) that they use to invade the extracellular matrix and attract surrounding wild-type endothelial and immune cells. Further, we demonstrate that this SASP is driven by the cytoskeletal, molec...
Source: Angiogenesis - August 3, 2021 Category: Molecular Biology Source Type: research
Successful management of lung adenocarcinoma with ALK/EGFR co-alterations and PD-L1 over-expression by bevacizumab combined with chemotherapy
We describe a case of advanced lung adenocarcinoma, harboring concurrent ALK/EGFR mutations and extremely high PD-L1 expression, that achieved sustained remission by the first-line treatment strategy of antiangiogenic therapy combined with chemotherapy. It is our firm conviction that the use anti-angiogenics should not have fallen out of favor in this era of targeted therapy and checkpoint inhibitors. (Source: Angiogenesis)
Source: Angiogenesis - August 3, 2021 Category: Molecular Biology Source Type: research
CCM2-deficient endothelial cells undergo a ROCK-dependent reprogramming into senescence-associated secretory phenotype
AbstractCerebral cavernous malformation (CCM) is a cerebrovascular disease in which stacks of dilated haemorrhagic capillaries form focally in the brain. Whether and how defective mechanotransduction, cellular mosaicism and inflammation interplay to sustain the progression of CCM disease is unknown. Here, we reveal that CCM1- and CCM2-silenced endothelial cells expanded in vitro enter into senescence-associated secretory phenotype (SASP) that they use to invade the extracellular matrix and attract surrounding wild-type endothelial and immune cells. Further, we demonstrate that this SASP is driven by the cytoskeletal, molec...
Source: Angiogenesis - August 3, 2021 Category: Molecular Biology Source Type: research
