Peritumoral B cells drive proangiogenic responses in HMGB1-enriched esophageal squamous cell carcinoma
AbstractSeveral B-cell subsets with distinct functions and polarized cytokine profiles that extend beyond antibody production have been reported in different cancers. Here we have demonstrated that proliferating B cells were predominantly found in the peritumoral region of esophageal squamous cell carcinoma (ESCC). These B cells were enriched in tumor nests with high expression of high-mobility group box 1 (HMGB1). High densities of peritumoral proliferating B cells and concomitantly high intratumoral HMGB1 expression showed improved prognostic significance, surpassing prognostic stratification of ESCC patients based on HM...
Source: Angiogenesis - October 6, 2021 Category: Molecular Biology Source Type: research
Blood vessels sense dermal stiffness via a novel mechanotransducer, APJ
AbstractMicrovascular dysfunction accompanied by a dramatic alteration of stable capillary structure is a major hallmark of numerous age-related diseases. In skin, although the role of angiogenesis during dermal reconstitution is well documented, the functional relevance of the extracellular matrix (ECM) stiffness to vascular remodeling and its molecular mechanisms was poorly understood. Here, we developed an ex vivo 3-dimensional angiogenic model using human fat, revealing that “appropriate” stiffness induces vascular maturation associated with upregulated APJ expression, whereas the overexpression of APJ promotes the...
Source: Angiogenesis - October 6, 2021 Category: Molecular Biology Source Type: research
Notch regulates vascular collagen IV basement membrane through modulation of lysyl hydroxylase 3 trafficking
AbstractCollagen type IV (Col IV) is a basement membrane protein associated with early blood vessel morphogenesis and is essential for blood vessel stability. Defects in vascular Col IV deposition are the basis of heritable disorders, such as small vessel disease, marked by cerebral hemorrhage and drastically shorten lifespan. To date, little is known about how endothelial cells regulate the intracellular transport and selective secretion of Col IV in response to angiogenic cues, leaving a void in our understanding of this critical process. Our aim was to identify trafficking pathways that regulate Col IV deposition during...
Source: Angiogenesis - October 4, 2021 Category: Molecular Biology Source Type: research
Pericyte hypoxia-inducible factor-1 (HIF-1) drives blood-brain barrier disruption and impacts acute ischemic stroke outcome
AbstractPericytes play essential roles in blood-brain barrier integrity and their dysfunction is implicated in neurological disorders such as stroke although the underlying mechanisms remain unknown. Hypoxia-inducible factor-1 (HIF-1), a master regulator of injury responses, has divergent roles in different cells especially during stress scenarios. On one hand HIF-1 is neuroprotective but on the other it induces vascular permeability. Since pericytes are critical for barrier stability, we asked if pericyte HIF-1 signaling impacts barrier integrity and injury severity in a mouse model of ischemic stroke. We show that pericy...
Source: Angiogenesis - October 4, 2021 Category: Molecular Biology Source Type: research
COVID-19 is a systemic vascular hemopathy: insight for mechanistic and clinical aspects
AbstractCoronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is presenting as a systemic disease associated with vascular inflammation and endothelial injury. Severe forms of SARS-CoV-2 infection induce acute respiratory distress syndrome (ARDS) and there is still an ongoing debate on whether COVID-19 ARDS and its perfusion defect differs from ARDS induced by other causes. Beside pro-inflammatory cytokines (such as interleukin-1 β [IL-1β] or IL-6), several main pathological phenomena have been seen because of endothelial cell (EC) dysfunction: hypercoagulation reflect...
Source: Angiogenesis - October 4, 2021 Category: Molecular Biology Source Type: research
Characterization of the heterogeneity of endothelial cells in bleomycin-induced lung fibrosis using single-cell RNA sequencing
AbstractThe loss of normal alveolar capillary and deregulated angiogenesis occurs simultaneously in idiopathic pulmonary fibrosis (IPF); however the contributions of specific endothelial subpopulations in the development of pulmonary fibrosis are poorly understood. Herein, we perform single-cell RNA sequencing to characterize the heterogeneity of endothelial cells (ECs) in bleomycin (BLM)-induced lung fibrosis in rats. One subpopulation, characterized by the expression of Nos3 and Cav1, is mostly distributed in non-fibrotic lungs and also highly expresses genes related to the “response to mechanical stimulus” and “lu...
Source: Angiogenesis - October 4, 2021 Category: Molecular Biology Source Type: research
High endothelial venules (HEVs) in immunity, inflammation and cancer
AbstractHigh endothelial venules (HEVs) are specialized blood vessels mediating lymphocyte trafficking to lymph nodes (LNs) and other secondary lymphoid organs. By supporting high levels of lymphocyte extravasation from the blood, HEVs play an essential role in lymphocyte recirculation and immune surveillance for foreign invaders (bacterial and viral infections) and alterations in the body ’s own cells (neoantigens in cancer). The HEV network expands during inflammation in immune-stimulated LNs and is profoundly remodeled in metastatic and tumor-draining LNs. HEV-like blood vessels expressing high levels of the HEV-speci...
Source: Angiogenesis - October 4, 2021 Category: Molecular Biology Source Type: research
Von Willebrand factor multimers during non-invasive ultrasound therapy for aortic valve stenosis
(Source: Angiogenesis)
Source: Angiogenesis - October 4, 2021 Category: Molecular Biology Source Type: research
Correction to: Notch regulates vascular collagen IV basement membrane through modulation of lysyl hydroxylase 3 trafficking
A correction to this paper has been published: https://doi.org/10.1007/s10456-021-09801-w (Source: Angiogenesis)
Source: Angiogenesis - October 4, 2021 Category: Molecular Biology Source Type: research
Opposite response of lung adenocarcinoma and its choroidal metastases upon ramucirumab plus docetaxel therapy after immunotherapy: a case report
We report a unique case of advanced non-small cell lung cancer that exhibited the opposite response to its unilateral choroidal metastases upon ramucirumab plus docetaxel treatment. A combination of cisplatin, pemetrexed, and pembrolizumab was administered as first-line treatment, resulting in shrinkage of all the lesions. However, although the patient was continued on a course of pembrolizumab, all the lesions had recurred approximately two months later. Ramucirumab plus docetaxel, administered as sequential treatment, resulted in maintained shrinkage of the choroidal lesions, yet all the other lesions progressed. Ramucir...
Source: Angiogenesis - September 30, 2021 Category: Molecular Biology Source Type: research
Chemerin regulates normal angiogenesis and hypoxia-driven neovascularization
AbstractChemerin is a multifunctional protein initially characterized in our laboratory as a chemoattractant factor for leukocyte populations. Its main functional receptor is CMKLR1. We identified previously chemerin as an anti-tumoral factor inhibiting the vascularization of tumor grafts. We show here that overexpression of bioactive chemerin in mice results in a reduction of the density of the retinal vascular network during its development and in adults. Chemerin did not affect vascular sprouting during the post-natal development of the network, but rather promoted endothelial cell apoptosis and vessel pruning. This phe...
Source: Angiogenesis - September 15, 2021 Category: Molecular Biology Source Type: research
Resident vascular endothelial progenitor definition and function: the age of reckoning
AbstractThe cardiovascular system is composed around the central function of the endothelium that lines the inner surfaces of its vessels. In recent years, the existence of a progenitor population within the endothelium has been validated through the study of endothelial colony-forming cells (ECFCs) in human peripheral blood and certain vascular beds. However, our knowledge on endothelial populationsin vivo that can give rise to ECFCs in culture has been limited. In this review we report and analyse recent attempts at describing progenitor populationsin vivo from murine studies that reflect the self-renewal and stemness ca...
Source: Angiogenesis - September 9, 2021 Category: Molecular Biology Source Type: research
