Low-abundance proteins-based label-free SERS approach for high precision detection of liver cancer with different stages
Anal Chim Acta. 2024 May 22;1304:342518. doi: 10.1016/j.aca.2024.342518. Epub 2024 Mar 21.ABSTRACTBACKGROUND: Surface-enhanced Raman scattering (SERS) technology have unique advantages of rapid, simple, and highly sensitive in the detection of serum, it can be used for the detection of liver cancer. However, some protein biomarkers in body fluids are often present at ultra-low concentrations and severely interfered with by the high-abundance proteins (HAPs), which will affect the detection of specificity and accuracy in cancer screening based on the SERS immunoassay. Clearly, there is a need for an unlabeled SERS method ba...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Tong Sun Yamin Lin Yun Yu Siqi Gao Xingen Gao Hongyi Zhang Kecan Lin Juqiang Lin Source Type: research
Ti < sub > 3 < /sub > C < sub > 2 < /sub > T < sub > x < /sub > /laser-induced graphene-based micro-droplet electrochemical sensing platform for rapid and sensitive detection of benomyl
In this study, flexible three-dimensional porous graphene electrode devices were first facilely fabricated using common laser ablation technique at room temperature. After then, hydrophilic two-dimensional MXene (Ti3C2Tx) nanosheet was decorated on the surface of the laser-induced graphene (LIG), resulting in disposable Ti3C2Tx/LIG electrode devices. After introducing Ti3C2Tx nanosheet, the electrochemical active area, electron transfer ability of LIG electrode device and its adsorption efficiency toward organic pesticide benomyl was significantly boosted. As a result, the fabricated Ti3C2Tx/LIG electrode device exhibited ...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Zhaohao Wang Mei Liu Shenchao Shi Xin Zhou Can Wu Kangbing Wu Source Type: research
Simultaneous and on-line detection of organic and heavy metal components in water using a novel nebulization-assisted injection plasma ionization triple quadruple mass spectrometry instrument
In this study, an analytical technique of nebulization-assisted injection plasma ionization mass spectrometry (NI-PIMS) was developed. This novel method enables the simultaneous detection of heavy metals and organic compounds in water system with high sensitivity, which has been demonstrated by the limit of quantification (LOQ) values below 1.0 μg/L for the three sterols (Enrofloxacin, ciprofloxacin, and clenbuterol) and three heavy metals (Pb, Ba, and Cd). Moreover, the method was successfully applied to rapidly analyze real water samples from urban and rural areas in China. The analytical results are available in less t...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Gaosheng Zhao Maolin Yang Tingting Zhang Bin Jia Li Xu Ping Cheng Source Type: research
Development of a LC-QTOF-MS based dilute-and-shoot approach for the botanical discrimination of honeys
In this study, a 'dilute-and-shoot' approach using liquid chromatography (LC) coupled to quadrupole time-of-flight-mass spectrometry (QTOF-MS) was applied to the non-targeted fingerprinting of honeys of different floral origin (buckwheat, clover and blueberry). This work investigated for the first time the impact of different instrumental conditions such as the column type, the mobile phase composition, the chromatographic gradient, and the MS fragmentor voltage (in-source collision-induced dissociation) on the botanical classification of honeys as well as the data quality. Results indicated that the data sets obtained for...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Lei Tian Shaghig Bilamjian Lan Liu Caren Akiki Daniel J Cuthbertson Tarun Anumol St éphane Bayen Source Type: research
Overcoming the detrimental O-acylation in TMTpro labeling improves the proteome depth and quantification precision
We presented a novel TMTpro labeling method that overcomes the detrimental O-acylation and thus significantly improves the depth and quantitative precision for proteome analysis.PMID:38637049 | DOI:10.1016/j.aca.2024.342538 (Source: Analytica Chimica Acta)
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Yan Cai Chenchen Chang Rijing Liao Source Type: research
A smartphone-based diagnostic analyzer for point-of-care milk somatic cell counting
Anal Chim Acta. 2024 May 22;1304:342540. doi: 10.1016/j.aca.2024.342540. Epub 2024 Apr 3.ABSTRACTBACKGROUND: Mastitis, a pervasive and detrimental disease in dairy farming, poses a significant challenge to the global dairy industry. Monitoring the milk somatic cell count (SCC) is vital for assessing the incidence of mastitis and the quality of raw cow's milk. However, existing SCC detection methods typically require large-scale instruments and specialized operators, limiting their application in resource-constrained settings such as dairy farms and small-scale labs. To address these limitations, this study introduces a nov...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Xiaoyun Sun Ruiming Zhao Xianhua Wang Yunlong Wu Degang Yang Jianhui Wang Zhihong Wu Nan Wang Juan Zhang Bin Xiao Jiaci Chen Fengchun Huang Ailiang Chen Source Type: research
Experimental investigation of confinement effect in single molecule amplification via real-time digital PCR on a multivolume droplet array SlipChip
In this study, we experimentally investigated for the first time the confinement effect in the community of digital PCR via a new real-time digital PCR system with a multivolume droplet array SlipChip (Muda-SlipChip). And a wider dynamic range of quantification methods compared to conventional digital PCR was validated by this system. This system provides emerging opportunities for life science research and clinical diagnostics. (63).PMID:38637051 | DOI:10.1016/j.aca.2024.342541 (Source: Analytica Chimica Acta)
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Yang Luo Qixin Hu Yan Yu Weiyuan Lyu Feng Shen Source Type: research
Identification of heavily glycated proteoforms by hydrophilic-interaction liquid chromatography and native size-exclusion chromatography - High-resolution mass spectrometry
In this study, we investigated two novel LC-HRMS methods to study glycated reference proteins at the intact protein level: low-flow hydrophilic-interaction liquid chromatography (HILIC) and native size-exclusion chromatography (SEC). Model proteins were exposed to conditions that favored extensive glycation and the formation of AGEs. After glycation, complicated MS spectra were observed, along with a sharply reduced signal response, possibly due to protein denaturation and the formation of aggregates. When using HILIC-MS, the glycated forms of the proteins could be resolved based on the number of reducing monosaccharides. ...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Ziran Zhai Peter J Schoenmakers Andrea F G Gargano Source Type: research
Colorimetric and SERS dual-mode detection of GSH in human serum based on AuNPs@Cu-porphyrin MOF nanozyme
In this study, gold nanoparticles (AuNPs) were synthesized in situ on the surface of two-dimensional Cu-porphyrin metal-organic framework (MOF) nanosheets to produce the AuNPs@Cu-porphyrin MOF nanozyme, which exhibited both oxidase-like activity and SERS detection ability. On one hand, the intrinsic oxidase-like activity of the nanozyme could be inhibited due to the chelation of glutathione (GSH) and Cu, which thus led to the visual color change of the solution. On the other hand, the abundant Raman "hot spots" at the nanogap generated by Au NPs and the internal standard (IS) signal provided by Cu-meso-tetra (4-carboxyphen...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Kexi Sun Chao Liu Yi Cao Jianxia Zhu Jialin Li Qing Huang Source Type: research
Label-free and portable detection of HIV-DNA by a handheld luminometer
Anal Chim Acta. 2024 May 22;1304:342553. doi: 10.1016/j.aca.2024.342553. Epub 2024 Mar 27.ABSTRACTBACKGROUND: The human immunodeficiency virus (HIV) remains a major worldwide health problem. Nowadays, many methods have been developed for quantitative detecting human immunodeficiency virus DNA (HIV-DNA), such as fluorescence and colorimetry. However, these methods still have the disadvantages of being expensive and requiring professional technicians. Early diagnosis of pathogens is increasingly dependent on portable instruments and simple point-of-care testing (POCT). Therefore, it is meaningful and necessary to develop por...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Mengmeng Wu Bing Yang Lu Shi Qiaorong Tang Jing Wang Wei Liu Baoxin Li Yan Jin Source Type: research
A novel photoelectrochemical self-screening aptamer biosensor based on CAU-17-derived Bi < sub > 2 < /sub > WO < sub > 6 < /sub > /Bi < sub > 2 < /sub > S < sub > 3 < /sub > for rapid detection of quorum sensing signal molecules
Anal Chim Acta. 2024 May 22;1304:342558. doi: 10.1016/j.aca.2024.342558. Epub 2024 Mar 30.ABSTRACTQuorum sensing signal molecule is an important biomarker released by some microorganisms, which can regulate the adhesion and aggregation of marine microorganisms on the surface of engineering facilities. Thus, it is significant to exploit a convenient method that can effectively monitor the formation and development of marine biofouling. In this work, an advanced photoelectrochemical (PEC) aptamer biosensing platform was established and firstly applied for the rapid and ultrasensitive determination of N-(3-Oxodecanoyl)-l-homo...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Shiliang Wang Peng Ju Weixing Liu Jingtian Chi Tiantong Jiang Zhe Chi Shuai Wang Ri Qiu Chengjun Sun Source Type: research
An α-helical peptide-based plasmonic biosensor for highly specific detection of α-synuclein toxic oligomers
We describe the integration of the peptide in a lab-on-a-chip plasmonic platform suitable for point-of-care measurements of αS toxic oligomers in CSF samples in real-time and at an affordable cost, providing an innovative biosensor for PD early diagnosis in the clinic.PMID:38637056 | DOI:10.1016/j.aca.2024.342559 (Source: Analytica Chimica Acta)
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Juliana F átima Giarola Jaime Santos M-Carmen Estevez Salvador Ventura Irantzu Pallar ès Laura M Lechuga Source Type: research
Confined DNA tetrahedral molecular sieve for size-selective electrochemiluminescence sensing
Anal Chim Acta. 2024 May 22;1304:342561. doi: 10.1016/j.aca.2024.342561. Epub 2024 Mar 30.ABSTRACTSize selectivity is crucial in highly accurate preparation of biosensors. Herein, we described an innovative electrochemiluminescence (ECL) sensing platform based on the confined DNA tetrahedral molecular sieve (DTMS) for size-selective recognition of nucleic acids and small biological molecule. Firstly, DNA template (T) was encapsulated into the inner cavity of DNA tetrahedral scaffold (DTS) and hybridized with quencher (Fc) labeled probe DNA to prepare DTMS, accordingly inducing Ru(bpy)32+ and Fc closely proximate, resulting...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Shiqiang Li Mingyu Leng Zongbing Li Qiumei Feng Xiangmin Miao Source Type: research
Chemiluminescence and electrochemiluminescence of water-soluble iridium(III) complexes containing a tetraethylene-glycol functionalised triazolylpyridine ligand
Anal Chim Acta. 2024 May 22;1304:342470. doi: 10.1016/j.aca.2024.342470. Epub 2024 Mar 22.ABSTRACTBACKGROUND: Iridium(III) complexes, exhibiting high luminescence quantum yields and a wide range of emission colours, are promising alternatives to tris(2,2'-bipyridine)ruthenium(II) for chemiluminescence (CL) and electrochemiluminescence (ECL) detection. This emerging class of reagent, however, is limited by the poor solubility of many iridium(III) complexes in aqueous solution, and lack of understanding of their remarkably variable selectivities towards different analytes.RESULTS: Seven [Ir(C^N)2(pt-TEG)]+ complexes, exhibit...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Lifen Chen Kim Quayle Zoe M Smith Timothy U Connell Egan H Doeven David J Hayne Jacqui L Adcock David J D Wilson Johnny Agugiaro Michael L Pattuwage Natasha S Adamson Paul S Francis Source Type: research
Split activator of CRISPR/Cas12a for direct and sensitive detection of microRNA
Anal Chim Acta. 2024 May 15;1303:342477. doi: 10.1016/j.aca.2024.342477. Epub 2024 Mar 22.ABSTRACTCRISPR/Cas12a-based nucleic acid assays have been increasingly used for molecular diagnostics. However, most current CRISPR/Cas12a-based RNA assays require the conversion of RNA into DNA by preamplification strategies, which increases the complexity of detection. Here, we found certain chimeric DNA-RNA hybrid single strands could activate the trans-cleavage activity of Cas12a, and then discovered the activating effect of split ssDNA and RNA when they are present simultaneously. As proof of concept, split nucleic acid-activated...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Wen He Xinyu Li Xinmin Li Minghui Guo Mengxuan Zhang Ruiwei Hu Menghan Li Shijia Ding Yurong Yan Source Type: research
