An electrochemical biosensor for the amplification of thrombin activity by perylene-mediated photoinitiated polymerization
Anal Chim Acta. 2024 May 8;1302:342494. doi: 10.1016/j.aca.2024.342494. Epub 2024 Mar 20.ABSTRACTBACKGROUND: Thrombin, a coagulation system protease, is a key enzyme involved in the coagulation cascade and has been developed as a marker for coagulation disorders. However, the methods developed in recent years have the disadvantages of complex operation, long reaction time, low specificity and sensitivity. Meanwhile, thrombin is at a lower level in the pre-disease period. Therefore, to accurately diagnose the disease, it is necessary to develop a fast, simple, highly sensitive and specific method using signal amplification ...
Source: Analytica Chimica Acta - April 5, 2024 Category: Chemistry Authors: Shuaibing Yu Jingliang Liu Lianzhi Li Kefeng Ma Jinming Kong Xueji Zhang Source Type: research
A comprehensive guide to extract information from extracellular vesicles: a tutorial review towards novel analytical developments
Anal Chim Acta. 2024 May 8;1302:342473. doi: 10.1016/j.aca.2024.342473. Epub 2024 Mar 16.ABSTRACTIn the medical field, extracellular vesicles (EVs) are gaining importance as they act as cells mediators. These are phospholipid bilayer vesicles and contain crucial biochemical information about their mother cells being carrier of different biomolecules such as small molecules, proteins, lipids, and nucleic acids. After release into the extracellular matrix, they enter the systemic circulation and can be found in all human biofluids. Since EVs reflect the state of the cell of origin, there is exponential attention as potential...
Source: Analytica Chimica Acta - April 5, 2024 Category: Chemistry Authors: Sabrina Roman ò Valeria Nele Virginia Campani Giuseppe De Rosa Stefano Cinti Source Type: research
Specific quantitative detection of N < sup > 6 < /sup > -methyladenosine at single-base resolution by extension-based isothermal exponential amplification reaction (E-IEXPAR)
Anal Chim Acta. 2024 May 8;1302:342474. doi: 10.1016/j.aca.2024.342474. Epub 2024 Mar 13.ABSTRACTBACKGROUND: N6-methyladenosine (m6A) is a common modification in RNA, crucial for various cellular functions and associated with human diseases. Quantification of m6A at single-base resolution is of great significance for exploring its biological roles and related disease research. However, existing analysis techniques, such as polymerase chain reaction (PCR) or loop-mediated isothermal amplification (LAMP), face challenges like the requirement for thermal cycling or intricate primer design. Therefore, it is urgent to establish...
Source: Analytica Chimica Acta - April 5, 2024 Category: Chemistry Authors: Fengxia Su Weiliang Liu Kejian Gao Desheng Chen Yongqiang Cheng Zhengping Li Source Type: research
Real-time MBDi-RPA using methyl-CpG binding protein 2: A real-time detection method for simple and rapid estimation of CpG methylation status
In this study, MBDi-RPA was performed using methyl-CpG binding protein 2 (MeCP2), another MBD family protein, as the blocking agent.RESULTS: MBDi-RPA using MeCP2 detected low levels of CpG methylation, showing that it had higher sensitivity than MBDi-RPA using MBD2. We also developed real-time RPA, which enabled rapid analysis of DNA amplification without the need for laborious agarose gel electrophoresis and used it in combination with MBDi-RPA. We termed this method real-time MBDi-RPA. The method using MeCP2 could determine the abundance ratio of CpG-methylated target DNA simply and rapidly, although highly sensitive det...
Source: Analytica Chimica Acta - April 5, 2024 Category: Chemistry Authors: Mina Ishidoya Toshitsugu Fujita Sadatomo Tasaka Hodaka Fujii Source Type: research
A green approach for the automatic quantitative analysis of additives in plastic samples using in-tube extraction dynamic headspace sampling technique coupled to GC-MS/MS
Anal Chim Acta. 2024 May 8;1302:342487. doi: 10.1016/j.aca.2024.342487. Epub 2024 Mar 21.ABSTRACTBACKGROUND: Many of the chemicals frequently used as additives have been recognised as hazardous substances, and therefore their analysis is necessary to evaluate plastic contamination risk. Additives analysis in plastic samples is usually performed by methods involving high volumes of toxic solvents or having high detection limits. In this work, a novel, fast, solventless and reliable green method was developed for the automated analysis of plastic additives from plastic samples. The proposed method consists of in-tube extract...
Source: Analytica Chimica Acta - April 5, 2024 Category: Chemistry Authors: Estefan ía Concha-Graña Carmen M Moscoso-P érez Ver ónica Fernández-González Purificaci ón López-Mahía Soledad Muniategui-Lorenzo Source Type: research
Efficient DNA walker guided by ordered cruciform-shaped DNA track for ultrasensitive and rapid electrochemical detection of lead ion
Anal Chim Acta. 2024 May 8;1302:342492. doi: 10.1016/j.aca.2024.342492. Epub 2024 Mar 16.ABSTRACTThe rational design of DNA tracks is an effective pathway to guide the autonomous movement and high-efficiency recognition in DNA walkers, showing outstanding advantages for the cascade signal amplification of electrochemical biosensors. However, the uncontrolled distance between two adjacent tracks on the electrode could increase the risk of derailment and interruption of the reaction. Hence, a novel four-way balanced cruciform-shaped DNA track (C-DNT) was designed as a structured pathway to improve the effectiveness and stabi...
Source: Analytica Chimica Acta - April 5, 2024 Category: Chemistry Authors: Nuanfei Zhu Kaixuan Wang Dinghui Xiong Jiaxuan Xiao Yibin Deng Zhugen Yang Zhen Zhang Source Type: research
Building a stable and robust anti-interference DNA dissipation system by eliminating the accumulation of systemic specified errors
In this study, we introduce strategically designed "trash cans" to facilitate kinetic control over interferences, transforming the stochastic binding of individual components within a homogeneous solution into a competitive binding process. This approach effectively eliminates incorrect binding and the accumulation of systemic interferences while ensuring a consistent pattern of energy fluctuation from response to silence. Remarkably, even in the presence of numerous interferences differing by only one base, we successfully achieve complete system reset through multiple cycles, effectively restoring the energy level to a m...
Source: Analytica Chimica Acta - April 5, 2024 Category: Chemistry Authors: Luojia Wang Wang Luo Zhi Weng Zhongzhong Wang You Wu Rong Zhao Xiaole Han Xin Liu Jianhong Zhang Yujun Yang Guoming Xie Source Type: research
A rapid and sensitive fluorescent chromatography with cloud system for MPXV point-of-care diagnosis
Anal Chim Acta. 2024 May 8;1302:342514. doi: 10.1016/j.aca.2024.342514. Epub 2024 Mar 21.ABSTRACTMonkeypox (mpox) is spreading around the world, and its rapid diagnosis is of great significance. In the present study, a rapid and sensitive fluorescent chromatography assisted with cloud system was developed for point-of-care diagnosis of mpox. To screen high affinity antibodies, nanoparticle antigen AaLS-A29 was generated by conjugating A29 onto scaffold AaLS. Immunization with AaLS-A29 induced significantly higher antibody titers and monoclonal antibodies were generated with the immunized mice. A pair of monoclonal antibodi...
Source: Analytica Chimica Acta - April 5, 2024 Category: Chemistry Authors: Haozhen Yan JiaYue Su Lvbo Tian Qianlin Li XiangNing Feng Jinsong Zhang Ying Shi Conghui Liao Jinsong Liu Shan Gao Mingwei Yang Xinrui Liu Jiahai Lu Zeliang Chen Source Type: research
Visually evaluating drug efficacy in living cells using COF-based fluorescent nanoprobe via CHA amplified detection of miRNA and simultaneous apoptosis imaging
Anal Chim Acta. 2024 May 8;1302:342502. doi: 10.1016/j.aca.2024.342502. Epub 2024 Mar 19.ABSTRACTBACKGROUNDS: Cancer is a highly fatal disease which is close relative of miRNA aberrant expression and apoptosis disorders. Elucidation of the therapeutic efficacy through investigating the changes in miRNA and apoptosis holds immense importance in advancing the development of miRNA-based precision therapy. However, it remains a challenge as how to visually evaluate the efficacy during protocol optimization of miRNA-based anticancer drugs at the cellular level. Therefore, exploring effective and noninvasive methods for real-tim...
Source: Analytica Chimica Acta - April 5, 2024 Category: Chemistry Authors: Chuandong Ge Zhe Chen Heming Sun Ping Sun Jiayin Zhao Yanjuan Wu Jing Xu Mingyang Zhou Mingming Luan Source Type: research
Exquisite visualization of mitophagy and monitoring the increase of lysosomal micro-viscosity in mitophagy with an unusual pH-independent lysosomal rotor
In this study, we rationally prepared a pH-independent lysosome probe to reduce the optical interference in mitophagy, and thus the process of mitophagy could be directly monitored in dual color through cooperation between IVDI and MTR, depending on Förster resonance energy transfer mechanism. IVDI shows remarkable fluorescence enhancement toward the increase of viscosity, and the fluorescence barely changes when pH varies. Due to the sensitivity to viscosity, the probe can visualize micro-viscosity alterations in lysosomes without washing procedures, and it showed better imaging properties than LTR. Thanks to the inertia...
Source: Analytica Chimica Acta - April 5, 2024 Category: Chemistry Authors: Rui Yang Wei He Changxin Zhu Xifeng Yang Yawei Kuang Tao Zhu Jingyang Xu Yuang Zhao Tingwang Jiang Yushen Liu Mengmeng Wei Source Type: research
Sensitive electrochemical flow injection analysis of H < sub > 2 < /sub > O < sub > 2 < /sub > released from cells with a pass-through mode
This study revealed the potential of mesh electrodes in electrochemical flow injection analysis for cellular function and pathology and its possible extension in cell counting and on-line analysis.PMID:38580411 | DOI:10.1016/j.aca.2024.342516 (Source: Analytica Chimica Acta)
Source: Analytica Chimica Acta - April 5, 2024 Category: Chemistry Authors: Mei-Ling Chen Pu Qian Tian-Yu Xia Chun-Mei Yu Zeng-Qiang Wu Ning Bao Xiao-Lei Huo Source Type: research
Advanced "turn-on" colorimetric uranium platform based on the enhanced nanozyme activity of a donor-acceptor structured covalent organic framework
Anal Chim Acta. 2024 May 8;1302:342503. doi: 10.1016/j.aca.2024.342503. Epub 2024 Mar 19.ABSTRACTBACKGROUND: The increasing uranium containing wastes generated during uranium mining and finishing pose a huge threat to the environment and human health, and thus robust strategies for on-site monitoring of uranium pollutant are of great significance for environmental protection around uranium tailings.RESULTS: Herein, a facile "turn-on" colorimetric platform that can achieve uranium detection by spectrometry and naked eyes was developed based on the uranium-enhanced nanozyme activity of covalent organic framework (JUC-505). T...
Source: Analytica Chimica Acta - April 5, 2024 Category: Chemistry Authors: Sai Jin Xiao Jing Huang An Ting Qiu Guang Zhou Liu Li Zhang Ting Wu Ya Di Shi Jian-Ding Qiu Source Type: research
Tris buffer-accelerated ligand exchange rate for instant fluorescence detection of trivalent chromium ion
In this study, we report that the good's buffers containing amino and polyhydroxy groups greatly increase the ligand exchange rate of Cr3+. Using EDTA as a model coordinate ligand, the Tris buffer (100 mM, pH 7.0) showed the best acceleration effect among the eight buffers. It improved the rate constant ∼20-fold, shorten the half-time 19-fold, and lowered the activation energy ∼70% at 40 °C. The Tris buffer was then applied for sensor based on the Cr3+-binding induced fluorescence quenching of fluorescein (FAM)-labeled and single-stranded DNA (ssDNA), which shortened the assay time from 1 h to 1 min. The Tris buffer a...
Source: Analytica Chimica Acta - April 5, 2024 Category: Chemistry Authors: Nan Wang Qianni Gao Xiaoqi Li Jinming Li Xinhui Lou Source Type: research
An electrochemical biosensor for the amplification of thrombin activity by perylene-mediated photoinitiated polymerization
Anal Chim Acta. 2024 May 8;1302:342494. doi: 10.1016/j.aca.2024.342494. Epub 2024 Mar 20.ABSTRACTBACKGROUND: Thrombin, a coagulation system protease, is a key enzyme involved in the coagulation cascade and has been developed as a marker for coagulation disorders. However, the methods developed in recent years have the disadvantages of complex operation, long reaction time, low specificity and sensitivity. Meanwhile, thrombin is at a lower level in the pre-disease period. Therefore, to accurately diagnose the disease, it is necessary to develop a fast, simple, highly sensitive and specific method using signal amplification ...
Source: Analytica Chimica Acta - April 5, 2024 Category: Chemistry Authors: Shuaibing Yu Jingliang Liu Lianzhi Li Kefeng Ma Jinming Kong Xueji Zhang Source Type: research
A comprehensive guide to extract information from extracellular vesicles: a tutorial review towards novel analytical developments
Anal Chim Acta. 2024 May 8;1302:342473. doi: 10.1016/j.aca.2024.342473. Epub 2024 Mar 16.ABSTRACTIn the medical field, extracellular vesicles (EVs) are gaining importance as they act as cells mediators. These are phospholipid bilayer vesicles and contain crucial biochemical information about their mother cells being carrier of different biomolecules such as small molecules, proteins, lipids, and nucleic acids. After release into the extracellular matrix, they enter the systemic circulation and can be found in all human biofluids. Since EVs reflect the state of the cell of origin, there is exponential attention as potential...
Source: Analytica Chimica Acta - April 5, 2024 Category: Chemistry Authors: Sabrina Roman ò Valeria Nele Virginia Campani Giuseppe De Rosa Stefano Cinti Source Type: research
