SCREENES: Enhancing non-uniform sampling reconstruction for symmetrical NMR spectroscopy
Anal Chim Acta. 2024 May 15;1303:342510. doi: 10.1016/j.aca.2024.342510. Epub 2024 Mar 25.ABSTRACTBACKGROUND: Symmetrical NMR spectroscopy, such as Total Correlation Spectroscopy (TOCSY) and other homonuclear spectroscopy, displays symmetry in chemical shift but are generally not symmetrical in terms of intensity, which constitutes a pivotal branch of multidimensional NMR spectroscopy and offers a robust tool for elucidating the structures and dynamics of complex samples, particularly in the context of biological macromolecules. Non-Uniform Sampling (NUS) stands as a critical technique for accelerating multidimensional NMR...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Ze Fang Bo Chen Chengda Huang Yifei Yuan Yao Luo Liubin Wu Yida Chen Yuqing Huang Yu Yang Enping Lin Zhong Chen Source Type: research
Determination of fatty acid uptake and desaturase activity in mammalian cells by NMR-based stable isotope tracing
Anal Chim Acta. 2024 May 15;1303:342511. doi: 10.1016/j.aca.2024.342511. Epub 2024 Mar 21.ABSTRACTBACKGROUND: Mammalian cells both import exogenous fatty acids and synthesize them de novo. Palmitate, the end product of fatty acid synthase (FASN) is a substrate for stearoyl-CoA desaturases (Δ-9 desaturases) that introduce a single double bond into fatty acyl-CoA substrates such as palmitoyl-CoA and stearoyl-CoA. This process is particularly upregulated in lipogenic tissues and cancer cells. Tracer methodology is needed to determine uptake versus de novo synthesis of lipids and subsequent chain elongation and desaturation. ...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Penghui Lin James Sledziona Kubra B Akkaya-Colak Maria M Mihaylova Andrew N Lane Source Type: research
Novel CRISPR/SpRY system for rapid detection of CRISPR/Cas-mediated gene editing in rice
This study aimed to develop a novel and efficient method, termed the CRISPR/SpRY detection platform, for the rapid screening of CRISPR/Cas9-induced mutants based on CRISPR/SpRY-mediated in vitro cleavage using rice (Oryza sativa L.) samples genetically edited at the TGW locus as an example. We designed the workflow of the CRISPR/SpRY detection platform and conducted a feasibility assessment. Subsequently, we optimized the reaction system of CRISPR/SpRY, and developed a one-pot CRISPR/SpRY assay by integrating recombinase polymerase amplification (RPA). The sensitivity of the method was further verified using recombinant pl...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Zhixun Su Xiaofu Wang Xiaoyun Chen Lin Ding Xiaoqun Zeng Junfeng Xu Cheng Peng Source Type: research
Dual biomarkers-activatable hollow MnO < sub > 2 < /sub > -Based theranostic nanoplatform for efficient breast cancer-specific multisite fluorescence imaging and synergistic therapy
Anal Chim Acta. 2024 May 15;1303:342521. doi: 10.1016/j.aca.2024.342521. Epub 2024 Mar 22.ABSTRACTBACKGROUND: Theranostic nanoplatforms with integrated diagnostic imaging and multiple therapeutic functions play a vital role in precise diagnosis and efficient treatment for breast cancer, but unfortunately, these nanoplatforms are usually stuck in single-site imaging and single mode of treatment, causing unsatisfactory diagnostic and therapeutic efficiency. Herein, a dual biomarkers-activatable facile hollow mesoporous MnO2 (H-MnO2)-based theranostic nanoplatform, DNAzyme@H-MnO2-MUC1 aptamer (DHMM), was constructed for the s...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Juan Ning Guizhen Hu Tian Wu Yijun Zhao Yamin Nie Yanmei Zhou Source Type: research
Electronic waste analysis using laser-induced breakdown spectroscopy (LIBS) and X-ray fluorescence (XRF): Critical evaluation of data fusion for the determination of Al, Cu and Fe
In conclusion, this research represents an important step toward a more sustainable and efficient future for electronic waste recycling, signifying its relevance to global environmental welfare and resource conservation.PMID:38609264 | DOI:10.1016/j.aca.2024.342522 (Source: Analytica Chimica Acta)
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Dennis S Ferreira Fabiola M V Pereira Alejandro C Olivieri Edenir R Pereira-Filho Source Type: research
Semiconducting polymer dots based l-lactate sensor by enzymatic cascade reaction system
Anal Chim Acta. 2024 May 15;1303:342523. doi: 10.1016/j.aca.2024.342523. Epub 2024 Mar 23.ABSTRACTBACKGROUND: l-lactate detection is important for not only assessing exercise intensity, optimizing training regimens, and identifying the lactate threshold in athletes, but also for diagnosing conditions like L-lactateosis, monitoring tissue hypoxia, and guiding critical care decisions. Moreover, l-lactate has been utilized as a biomarker to represent the state of human health. However, the sensitivity of the present l-lactate detection technique is inadequate.RESULTS: Here, we reported a sensitive ratiometric fluorescent prob...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Shuyi He Weichao Liu Steven Xu Wu Source Type: research
Self-validating photothermal and electrochemical dual-mode sensing based on Hg < sup > 2+ < /sup > etching Ti < sub > 3 < /sub > C < sub > 2 < /sub > MXene
Anal Chim Acta. 2024 May 15;1303:342525. doi: 10.1016/j.aca.2024.342525. Epub 2024 Mar 23.ABSTRACTMercury ions can cause serious damage to the ecological environment, and it is necessary to develop reliable and elegant mercury ion sensors. In this protocol, a label-free photothermal/electrochemical dual-mode strategy for Hg2+ is proposed based on delaminated Ti3C2 MXene nanosheets (DL-Ti3C2 MXene). Hg2+ exists in water in the form of HgCl2, Hg(OH)2, and HgClOH, and the electron-rich elements O and Cl can specifically bind to the positively charged DL-Ti3C2 MXene at the edge, and further oxidation-reduction reaction occurs ...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Yanxin Zhang Jing Li Xiaobing Li Jingchun Lv Qin Xu Hongbo Li Source Type: research
Developing a novel strategy for fabricating matrix film to assess the distribution of potassium perfluorooctanic sulfonate by matrix-assisted laser desorption/ionization mass spectrometry imaging
Anal Chim Acta. 2024 May 15;1303:342528. doi: 10.1016/j.aca.2024.342528. Epub 2024 Mar 25.ABSTRACTMatrix deposition plays a critical role in image quality of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). To improve the ionization efficiency and overcome the limitation of traditional matrix deposition methods in the face of difficult-to-sublimate or difficult-to-dissolve matrix, covalent organic frameworks (COFs) named COF-DhaTab was successfully synthesized and firstly used as matrix film. It was fabricated by imprinting of sieved COF-DhaTab powder on the surface of a double-sided adhes...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Yake Luo Shanshan Ma Jianxun Zhang Qidong Zhang Yanhao Zhang Jian Mao Hang Yuan Gangfeng Ouyang Shusheng Zhang Wuduo Zhao Source Type: research
Target and suspect screening of psychoactive substances in seizures and oral fluid exploiting retention time prediction and LC-MS/MS analysis
Anal Chim Acta. 2024 May 15;1303:342529. doi: 10.1016/j.aca.2024.342529. Epub 2024 Mar 25.ABSTRACTBACKGROUND: Novel psychoactive substances (NPS) are a group of substances, mainly of synthetic origin, characterized by toxicological properties extremely dangerous. The main difficulty in recognizing NPS in seizures and biological samples lies in their dynamic nature, related to the continuous synthesis and introduction on the market of new drugs, often with very similar structures to existing ones. The aim of this study was the creation of a robust and versatile method for the analysis of traditional drugs and NPS in differe...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Gaia Di Francesco Flaminia Vincenti Camilla Montesano Ilenia Bracaglia Martina Croce Sabino Napoletano Antonietta Lombardozzi Manuel Sergi Source Type: research
Design and analysis of self-priming extension DNA hairpin probe for miRNA detection based on a unified dynamic programming framework
Anal Chim Acta. 2024 May 15;1303:342530. doi: 10.1016/j.aca.2024.342530. Epub 2024 Mar 25.ABSTRACTMicroRNAs (miRNAs) are potential biomarkers for cancer diagnosis and prognosis, methods for detecting miRNAs with high sensitivity, selectivity, and stability are urgently needed. Various nucleic acid probes that have traditionally been for this purpose suffer several drawbacks, including inefficient signal-to-noise ratios and intensities, high cost, and time-consuming method establishment. Computing tools used for investigating the thermodynamics of DNA hybridization reactions can accurately predict the secondary structure of...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Hecheng Wang Jiatong Liu Yanyan Fang Xudan Shen Hui Liu Lushan Yu Su Zeng Sheng Cai Jingjing Zhou Zheyong Li Source Type: research
MazF-rolling circle amplification combined MALDI-TOF MS for site-specific detection of N < sup > 6 < /sup > -methyladenosine RNA
Anal Chim Acta. 2024 May 15;1303:342532. doi: 10.1016/j.aca.2024.342532. Epub 2024 Mar 25.ABSTRACTN6-methyladenosine (m6A) is one of the most abundant chemical modifications in RNA and has vital significance in cellular processes and tumor development. However, the accurate analysis of site-specific m6A modification remains a challenge. In this work, a MazF endoribonuclease activated rolling circle amplification (MazF-RCA) combined MALDI-TOF MS assay is developed for the detection of site-specific m6A-RNA. MazF endoribonuclease can specifically cleave the ACA motif, leaving methylated (m6A)CA motif intact. The intact methy...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Guobin Han Qiuyuan Lin Jia Yi Qian Lyu Qingwei Ma Liang Qiao Source Type: research
Ionic liquid-functionalized mesoporous multipod silica for simultaneously effective extraction of aflatoxin B < sub > 1 < /sub > and its two precursors from grain
In this study, a bisimidazolium-type ionic liquid functionalized mesoporous multipod silica (SiO2@mPMO-IL(im)2) was fabricated to extract AFB1 and its two precursors, i.e., averantin and sterigmatocystin. The SiO2@mPMO-IL(im)2 could simultaneously extract three targets with varied structures based on the multipods, mesopores, and multifunctional groups. The density functional theory calculations further verified the multiple interactions between SiO2@mPMO-IL(im)2 and targets. The fabricated SiO2@mPMO-IL(im)2 could effectively extract and determine three targets in grains by combing with dispersive solid-phase extraction an...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Xiaxing Feng Yaming Sun Tao Zhang Jingna Li Hailiang Zhao Wenjie Zhao Guoqiang Xiang Lijun He Source Type: research
Quantifying payloads of antibody ‒drug conjugates using a postcolumn infused-internal standard strategy with LC‒MS
Anal Chim Acta. 2024 May 15;1303:342537. doi: 10.1016/j.aca.2024.342537. Epub 2024 Mar 25.ABSTRACTBACKGROUND: Antibody‒drug conjugates (ADCs) are innovative biopharmaceutics consisting of a monoclonal antibody, linkers, and cytotoxic payloads. Monitoring circulating payload concentrations has the potential to identify ADC toxicity; however, accurate quantification faces challenges, including low plasma concentrations, severe matrix effects, and the absence of stable isotope-labeled internal standards (SIL-IS) for payloads and their derivatives. Previous studies used structural analogs as internal standards, but different...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Chih-Ning Cheng Hsiao-Wei Liao Ching-Hung Lin Wen-Chi Chang I-Chun Chen Yen-Shen Lu Ching-Hua Kuo Source Type: research
ECL cytosensor for sensitive and label-free detection of circulating tumor cells based on hierarchical flower-like gold microstructures
Anal Chim Acta. 2024 May 15;1303:342505. doi: 10.1016/j.aca.2024.342505. Epub 2024 Mar 23.ABSTRACTThe development of sensitive and efficient cell sensing strategies to detect circulating tumor cells (CTCs) in peripheral blood is crucial for the early diagnosis and prognostic assessment of cancer clinical treatment. Herein, an array of hierarchical flower-like gold microstructures (HFGMs) with anisotropic nanotips was synthesized by a simple electrodeposition method and used as a capture substrate to construct an ECL cytosensor based on the specific recognition of target cells by aptamers. The complex topography of the HFGM...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Meixing Li Jiawei Shi Yuhang Zhang Shuaihui Cui Lei Zhang Qingming Shen Source Type: research
Construction of an autofluorescence interference-free phosphorescence biosensor for the specific detection of TK1 mRNA
In this study, a phosphorescent sensor was constructed, which can accurately detect thymidine kinase 1 (TK1) mRNA in biological samples and avoid autofluorescence interference. When there is no target, polydopamine (PDA) is used as the phosphorescence resonance energy transfer (PRET) acceptor to quench the phosphorescence of the persistently luminescent (PL) nanomaterial. When there is a target, the DNA modified by the PL nanomaterial is replaced by the hairpin H and removed away from the PDA, resulting in a rebound in phosphorescence. The phosphorescent sensor exhibits a good linear relationship in the TK1 mRNA concentrat...
Source: Analytica Chimica Acta - April 12, 2024 Category: Chemistry Authors: Hang Gong Shufen Yao Xiaojia Zhao Feng Chen Chunyan Chen Changqun Cai Source Type: research
