Isothermal strand displacement polymerase reaction (ISDPR)-assisted microchip electrophoresis for highly sensitive detection of cancer associated microRNAs
Anal Chim Acta. 2024 Apr 29;1300:342469. doi: 10.1016/j.aca.2024.342469. Epub 2024 Mar 12.ABSTRACTMore and more studies have found that microRNAs (miRNAs) are markers of cancer, and detection of miRNAs is beneficial for early diagnosis and prognosis of cancer. In this paper, the isothermal strand displacement polymerase reaction (ISDPR), which is an enzyme-assisted nucleic acid amplification method, was studied to combine with microchip electrophoresis (MCE) for a simultaneously detection of two cancer related miRNAs named microRNA-21 (miR-21) and microRNA-221 (miR-221). In the ISDPR amplification, two different DNA hairpi...
Source: Analytica Chimica Acta - March 23, 2024 Category: Chemistry Authors: Jingyi Chen Jingzi Zhang Qihui Xie Zhaohui Chu Yuqi Lu Fan Zhang Qingjiang Wang Source Type: research
Construction of a glycosylation-mediated fluorescent biosensor for label-free measurement of site-specific 5-hydroxymethylcytosine in cancer cells with zero background signal
Anal Chim Acta. 2024 Apr 29;1300:342463. doi: 10.1016/j.aca.2024.342463. Epub 2024 Mar 11.ABSTRACTBACKGROUND: 5-hydroxymethylcytosine (5hmC) as an epigenetic modification can regulate gene expression, and its abnormal level is related with various tumor invasiveness and poor prognosis. Nevertheless, the current methods for 5hmC assay usually involve expensive instruments/antibodies, radioactive risk, high background, laborious bisulfite treatment procedures, and non-specific/long amplification time.RESULTS: We develop a glycosylation-mediated fluorescent biosensor based on helicase-dependent amplification (HDA) for label-f...
Source: Analytica Chimica Acta - March 23, 2024 Category: Chemistry Authors: Wei Wang Su Jiang Yue-Ying Li Yun Han Meng Liu Ying-Ying Meng Chun-Yang Zhang Source Type: research
Analysis of the response to cigarette smoke exposure in cell coculture and monoculture based on bionic-lung microfluidic chips
Anal Chim Acta. 2024 Apr 29;1300:342446. doi: 10.1016/j.aca.2024.342446. Epub 2024 Mar 6.ABSTRACTBACKGROUND: In vitro toxicity assessment studies with various experimental models and exposure modalities frequently generate diverse outcomes. In the prevalent experimental, aerosol pollutants are dissolved in culture medium through capture for exposure to two-dimensional planar cellular models in multiwell plates via immersion. However, this approach can generate restricted and inconclusive experimental data, significantly constraining the applicability of risk assessment outcomes. Herein, the in vitro cocultivation of lung e...
Source: Analytica Chimica Acta - March 23, 2024 Category: Chemistry Authors: Zezhi Li Boyang Feng Xiang Li Junwei Zhao Kejian Liu Fuwei Xie Jianping Xie Source Type: research
First electrochemical investigation and determination of non-steroidal anti-inflammatory drug etofenamate using disposable pencil graphite electrode with voltammetric techniques
In this study, the electrochemical properties of etofenamate, an active ingredient belonging to the non-steroidal anti-inflammatory drug group, were investigated using cyclic voltammetry (CV) and square wave voltammetry (SW) techniques on a disposable pencil graphite electrode (PGE). With the CV technique, reversible voltammetric waves of around +0.470 V and irreversible voltammetric waves of around +1.02 V were produced on the PGE. An environmentally friendly, selective and highly sensitive SW voltammetric method was developed using disposable PGE. This voltammetric method gave very good analytical working range on PGE in...
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: Aysel Yurdem Mehmet Aslan Hayriye Aral Abdulkadir Levent Source Type: research
Differential detection of H1N1 virus spiker proteins by two hexaphenylbutadiene isomers based on size-matching principle
Anal Chim Acta. 2024 Apr 22;1299:342452. doi: 10.1016/j.aca.2024.342452. Epub 2024 Mar 5.ABSTRACTAs one of the high pathogenic influenza viruses, H1N1 virus easily induces to serious diseases, even leading to death. To date, all detection methods for H1N1 virus had shortcomings, including high equipment cost, time consumption, and etc. Therefore, a novel detection method should be established to achieve more convenient, rapid, and low-cost detection. In this work, an isomer of HPBmN-I with aggregation-induced emission characteristic was firstly synthesized on the basis of our previous reported HPBpN-I. The results showed t...
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: Xiaoling Pan Jian Wang Kai Zhang Peng Sun Jianbing Shi Junge Zhi Zhengxu Cai Zi Li Dayan Wang Bin Tong Yuping Dong Source Type: research
Triplex DNA-based aggregation-induced emission probe: A new platform for hybridization chain reaction-based fluorescence sensing assay
Anal Chim Acta. 2024 Apr 22;1299:342406. doi: 10.1016/j.aca.2024.342406. Epub 2024 Feb 27.ABSTRACTThe hybridization chain reaction (HCR), as one of the nucleic acid amplification technologies, is combined with fluorescence signal output with excellent sensitivity, simplicity, and stability. However, current HCR-based fluorescence sensing methods still have some defects such as the blocking effect of the HCR combination with fluorophores and the aggregation-caused quenching (ACQ) phenomenon of traditional fluorophores. Herein, a triplex DNA-based aggregation-induced emission probe (AIE-P) was designed as the fluorescent sig...
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: Yubin Li Ciling Li Chuangqiang Zhang Liting Zhao Yaoyun Huang Source Type: research
A high-throughput DNA analysis method based on isothermal amplification on a suspension microarray for detecting mpox virus and viruses with comparable symptoms
In this study, we combined suspension microarray technology with recombinase-aided amplification technology (RAA) to establish a high-throughput, sensitive, and quantitative method for detecting MPXV and other viruses that can cause similar symptoms. The experimental results confirmed that the technique has outstanding sensitivity, with a minimum detection limit (LOD) of 0.1 fM and a linear range of 0.3 fM to 20 pM, spanning five orders of magnitude. The approach also exhibits exquisite selectivity, as the amplified signal can only be detected when the target virus nucleic acid is present. Additionally, serum recoveries ra...
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: Liming Zhang Jieyu Liu Shisi Huang Wentao Zeng Li Li Xihao Fan Zhuoxuan Lu Source Type: research
A novel premixing strategy for highly sensitive detection of nitrite on paper-based analytical devices
This study presents a simple and novel strategy for the highly sensitive detection of nitrite in food using paper-based analytical devices (PADs). In this proposed strategy, the nitrite present in the sample undergoes efficient diazotization when initially mixed with sulfanilamide solution before reacting with N-(1-naphthyl) ethylenediamine dihydrochloride (NED) coated on the detection region of the PAD, leading to the maximum production of colored azo compounds. Specifically, within the concentration range of 0.1-20 mg/L, the LOD and LOQ for the nitrite assay using the premixing strategy are determined as 0.053 mg/L and 0...
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: Xiaohu Xu Azadeh Nilghaz Xiaofang Wan Shan Liu Meigui Xue Wan Guo Junfei Tian Source Type: research
Comparison of single and double pulse laser-induced breakdown spectroscopy for the detection of biomolecules tagged with photon-upconversion nanoparticles
Anal Chim Acta. 2024 Apr 22;1299:342418. doi: 10.1016/j.aca.2024.342418. Epub 2024 Feb 27.ABSTRACTBACKGROUND: Laser-induced breakdown spectroscopy (LIBS) is a well-recognized analytical technique used for elemental analysis. This method is gaining considerable attention also in biological applications thanks to its ability for spatial mapping and elemental imaging. The implementation of LIBS in the biomedical field is based on the detection of metals or other elements that either naturally occur in the samples or are present artificially. The artificial implementation of nanoparticle labels (Tag-LIBS) enables the use of LI...
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: Zden ěk Farka Karol ína Vytisková Ekaterina Makhneva Eva Zikmundov á Daniel Holub Jakub Buday David Prochazka Karel Novotn ý Petr Skl ádal Pavel Po řízka Jozef Kaiser Source Type: research
A triggered DNA nanomachine with enzyme-free for the rapid detection of telomerase activity in a one-step method
In conclusion, our study demonstrated that the triggered-DNA nanomachine fulfills the requirements for rapid detection of telomerase activity in one-step under isothermal and enzyme-free conditions with excellent specificity, and its simple and stable structure makes it ideal for complex systems. These findings indicated the application prospect of DNA nanomachines in clinical diagnostics and provided new insights into the field of DNA nanomachine-based bioanalysis.PMID:38499416 | DOI:10.1016/j.aca.2024.342420 (Source: Analytica Chimica Acta)
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: Huajie Pang Yanan Peng Rui Zhang Zhijun Gao Xiangde Lai Dongxia Li Xuan Zhao Yuanyuan Wang Hua Pei Bin Qiao Yuxiang Ji Qiang Wu Source Type: research
UiO-66-NH < sub > 2 < /sub > initiated cascade reaction: Constructing a ratiometric fluorescence sensor for ultrasensitive detection of nerve agent simulant
Anal Chim Acta. 2024 Apr 22;1299:342421. doi: 10.1016/j.aca.2024.342421. Epub 2024 Feb 27.ABSTRACTBACKGROUND: Highly toxic organophosphorus nerve agents often exist in the form of gas in the environment and can damage human neuroregulatory system by inhibiting the activity of acetylcholinesterase (AChE). However, fluorescent probes based on small organic molecules bring a secondary burden to environment, and their sensitivity and specificity for sarin simulant diethyl chlorophosphate (DCP) detection are unsatisfactory. Nanozyme cascade systems with signal amplification can be used for highly sensitive identification of ana...
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: You Lei Nan Gao Pengcheng Huang Fang-Ying Wu Source Type: research
miRNA and DNA analysis by negative ion electron transfer dissociation and infrared multiple-photon dissociation mass spectrometry
Anal Chim Acta. 2024 Apr 22;1299:342431. doi: 10.1016/j.aca.2024.342431. Epub 2024 Feb 27.ABSTRACTBACKGROUND: The use of simple and hybrid fragmentation techniques for the identification of molecules in tandem mass spectrometry provides different and complementary information on the structure of molecules. Nevertheless, these techniques have not been as widely explored for oligonucleotides as for peptides or proteins. The analysis of microRNAs (miRNAs) warrants special attention, given their regulatory role and their relationship with several diseases. The application of different fragmentation techniques will be very inte...
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: Miriam Guzm án-Lorite Fr édéric Rosu Mar ía Luisa Marina Mar ía Concepción García Val érie Gabelica Source Type: research
Three-dimensional DNA biomimetic networks (B-3D Net)-based ratiometric fluorescence platform for cancer-related gene biosensing
This study introduces a target induced three-dimensional DNA biomimetic networks (B-3D Net)-based ratiometric fluorescence platform using manganese dioxide nanosheets (MnO2 NS)/o-phenylenediamine in combination with hybridization chain reaction to detect cancer-related genes (p53 gene). The incorporation of multiple signals within the B-3D networks can significantly enhance catalytic activity and amplify the output signals, enabling a high sensitivity. Compared with traditional ratio fluorescence platforms, there is no demand to synthesize fluorescent nanoprobes due to the in-situ formation of fluorescence species, which i...
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: Chao Yao Guiqun Zhang Hongling Tao Yulong Li Rong Hu Yunhui Yang Source Type: research
A multifunctional fluorescent probe for sequential detection of hydrogen sulfide and pH in foodstuffs, living cells and mice
Anal Chim Acta. 2024 Apr 22;1299:342434. doi: 10.1016/j.aca.2024.342434. Epub 2024 Feb 28.ABSTRACTBACKGROUND: Cancer as a leading cause of premature death worldwide has become a major threat to human health due to the high incidence and mortality. Monitoring tumor markers are reliable and significantly important for early detection of cancers. In complex biological systems, it is of great urgency but still remains challenging to conceive a fluorescent probe with multiple tumor markers detection property. Hydrogen sulfide (H2S) and pH are two target biomarkers for diagnosis of early cancer. The preparation of a novel probe ...
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: Zu-Sheng Huang Wenxuan Zhang Manshan Liang Shihua Wang Zhongda Zhang Yongsheng Jiang Xiaoxia Ye Longteng Xie Yun-Yun Quan Source Type: research
Development of a water-soluble fluorescent Al < sup > 3+ < /sup > probe based on phenylsulfonyl-2-pyrone in biological systems
In this study, an Al3+-selective fluorescent probe PSP based on a novel pyrone molecule was synthesized and characterized to detect and track Al in biological systems. PSP exhibited fluorescence enhancement at 580 nm in the presence of Al3+ in aqueous media. Binding analysis using Job's plot and structural analysis using 1H NMR showed that PSP formed a 1:1 complex with Al3+ at the two carbonyl groups of the dimethyl malonate of the pyrone ring. Upon testing in biological systems, PSP showed good cell membrane permeability, detected intracellular Al3+ in human breast cancer cells (MDA-MB-231), and successfully imaged accumu...
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: Fumiko Hara Naoko Mizuyama Takeshi Fujino Ashok Kumar Shrestha Thenuwara Arachchige Omila Kasun Meetiyagoda Shinya Takada Hideo Saji Takahiro Mukai Masayori Hagimori Source Type: research
