In vitro selection of a trans aptamer complex for target-responsive fluorescence activation
Anal Chim Acta. 2024 May 1;1301:342465. doi: 10.1016/j.aca.2024.342465. Epub 2024 Mar 11.ABSTRACTBACKGROUND: Most biological molecular complexes consist of multiple functional domains, yet rationally constructing such multifunctional complexes is challenging. Aptamers, the nucleic acid-based functional molecules, can perform multiple tasks including target recognition, conformational changes, and enzymatic activities, while being chemically synthesizable and tunable, and thus provide a basis for engineering enhanced functionalities through combination of multiple units. However, the conventional approach of simply combinin...
Source: Analytica Chimica Acta - March 29, 2024 Category: Chemistry Authors: Soyeon V Park Byunghwa Kang Minjong Lee Hyebin Yoo Hyesung Jo Sungwook Woo Seung Soo Oh Source Type: research
Target mediated bioreaction to engineer surface vacancy effect on Bi < sub > 2 < /sub > O < sub > 2 < /sub > S nanosheets for photoelectrochemical detection of FEN1
Anal Chim Acta. 2024 May 1;1301:342467. doi: 10.1016/j.aca.2024.342467. Epub 2024 Mar 13.ABSTRACTPhotoelectrochemistry represents a promising technique for bioanalysis, though its application for the detection of Flap endonuclease 1 (FEN1) has not been tapped. Herein, this work reports the exploration of creating oxygen vacancies (Ov) in situ onto the surface of Bi2O2S nanosheets via the attachment of dopamine (DA), which underlies a new anodic PEC sensing strategy for FEN1 detection in label-free, immobilization-free and high-throughput modes. In connection to the target-mediated rolling circle amplification (RCA) reactio...
Source: Analytica Chimica Acta - March 29, 2024 Category: Chemistry Authors: Jiangwei Hu Xin Gao Mengmeng Gu Yuanyuan Sun Yuming Dong Guang-Li Wang Source Type: research
Quantifying exhaled acetone and isoprene through solid phase microextraction and gas chromatography-mass spectrometry
The objective of this study was to develop an accessible method to quantify acetone and isoprene in breath by SPME GC-MS.RESULTS: A system was developed to mimic human exhalation and expose VOCs to a SPME fiber in the gas phase at known concentrations. VOCs were bubbled/diluted with dry air at a fixed flow rate, duration, and volume that was comparable to a previously developed breath sampling method. Identification of acetone and isoprene through GC-MS was verified using standards and observing overlaps in chromatographic retention/mass spectral fragmentation. Calibration curves were developed for these two analytes, whic...
Source: Analytica Chimica Acta - March 29, 2024 Category: Chemistry Authors: Eray Schulz Mark Woollam Sneha Vashistha Mangilal Agarwal Source Type: research
β-Glucuronidase-triggered reaction for fluorometric and colorimetric dual-mode assay based on the in situ formation of silicon nanoparticles
This study provides a facile dual-mode fluorometric and colorimetric approach for determination of GUS activity based on novel Si NPs for the first time. This designed sensing approach was successfully employed for the quantification of GUS in human serum samples and screening of GUS inhibitors, indicating the feasibility and potential applications in clinical cancer diagnosis and anti-cancer drug discovery.PMID:38553126 | DOI:10.1016/j.aca.2024.342471 (Source: Analytica Chimica Acta)
Source: Analytica Chimica Acta - March 29, 2024 Category: Chemistry Authors: Yue Li Weiping Liu Xinxin Jiang Hongmei Liu Sikai Wang Xiaoqian Mao Ruyu Bai Yulu Wen Xiaojun Luo Guoqi Zhang Yan Zhao Source Type: research
Microfluidic device featuring micro-constrained channels for multi-parametric assessment of cellular biomechanics and high-precision mechanical phenotyping of gastric cells
This study provides a deep insight into the biophysical mechanism of gastric cancer metastasis at the single-cell level and possesses great potential to function as a valuable tool for single-cell analysis, thereby facilitating high-precision and high-throughput discrimination of cellular phenotypes that are not easily discernible through single-marker analysis.PMID:38553127 | DOI:10.1016/j.aca.2024.342472 (Source: Analytica Chimica Acta)
Source: Analytica Chimica Acta - March 29, 2024 Category: Chemistry Authors: Yang Heng Xinyu Zheng Youyuan Xu Jiaqi Yan Ying Li Lining Sun Hao Yang Source Type: research
A group-targeting biosensor for sensitive and rapid detection of quinolones in water samples
Anal Chim Acta. 2024 May 1;1301:342475. doi: 10.1016/j.aca.2024.342475. Epub 2024 Mar 13.ABSTRACTBACKGROUND: Quinolones (QNs) widely exist in the environment due to their wide range of applications and poor metabolic properties, resulting in the generation and spread of resistance genes, posing a potential threat to human health. Traditional analytical methods cannot detect all broad ranges of QNs simultaneously. The development of facile, efficient and reliable method for quantification and assessment of the total QNs is a long-lasting challenge.RESULTS: We hereby provide a simple, sensitive and instantaneous group-target...
Source: Analytica Chimica Acta - March 29, 2024 Category: Chemistry Authors: Bohan Wang Lanhua Liu Haopeng Zhang Zhiqiang Wang Kang Chen Bo Wu Limin Hu Xiaohong Zhou Lanlan Liu Source Type: research
Review: 3D cell models for organ-on-a-chip applications
Anal Chim Acta. 2024 May 1;1301:342413. doi: 10.1016/j.aca.2024.342413. Epub 2024 Feb 28.ABSTRACTTwo-dimensional (2D) cultures do not fully reflect the human organs' physiology and the real effectiveness of the used therapy. Therefore, three-dimensional (3D) models are increasingly used in bioanalytical science. Organ-on-a-chip systems are used to obtain cellular in vitro models, better reflecting the human body's in vivo characteristics and allowing us to obtain more reliable results than standard preclinical models. Such 3D models can be used to understand the behavior of tissues/organs in response to selected biophysica...
Source: Analytica Chimica Acta - March 29, 2024 Category: Chemistry Authors: Agnieszka Żuchowska Patrycja Baranowska Magdalena Flont Zbigniew Brz ózka El żbieta Jastrzębska Source Type: research
Solvent-free parallel artificial liquid membrane extraction for drugs of abuse in plasma samples using LC-MS/MS
In this study, the use of an EO provided a solvent-free sample preparation technique suited to extract different classes of drugs of abuse from plasma samples, dismissing the use of hazardous organic solvents. The method also provided excellent sample clean-up, thus being a simple and efficient tool for toxicological applications that is in agreement with the principles of sustainable chemistry.PMID:38553114 | DOI:10.1016/j.aca.2024.342387 (Source: Analytica Chimica Acta)
Source: Analytica Chimica Acta - March 29, 2024 Category: Chemistry Authors: Andr é Luis Fabris Stig Pedersen-Bjergaard Elisabeth Leere Øiestad Giordano Novak Rossi Jaime E Cec ílio Hallak Rafael Guimar ães Dos Santos Jose Luiz Costa Mauricio Yonamine Source Type: research
Flow field-flow fractionation and single particle inductively coupled plasma mass spectrometry as a powerful tool for tracking and understanding the sensing mechanism of Ag-Au bimetallic nanoparticles toward cobalt ions
This study illustrates the usefulness of SP-ICP-MS and FlFFF-ICP-MS in the nanoparticle-based sensor development research area.PMID:38553115 | DOI:10.1016/j.aca.2024.342485 (Source: Analytica Chimica Acta)
Source: Analytica Chimica Acta - March 29, 2024 Category: Chemistry Authors: Luluil Maknun Jitapa Sumranjit Tuksadon Wutikhun Ryszard Lobinski Joanna Szpunar Atitaya Siripinyanond Source Type: research
Novel method for chlorine determination in biofuel lipid feedstock by coupling a total sample introduction system (hTISIS) to an inductively coupled plasma tandem mass spectrometry (ICP-MS/MS)
Anal Chim Acta. 2024 May 1;1301:342414. doi: 10.1016/j.aca.2024.342414. Epub 2024 Feb 26.ABSTRACTBACKGROUND: Chlorine concentrations above 1 mg kg-1 in lipid feedstocks for biofuel production can generate corrosion issues in the different refining units as well as catalyst deactivation by clogging or fouling. To reach accurate analyses by inductively coupled plasma (ICP) techniques at low concentration levels, dilution in organic solvents rises as a simpler and more straightforward sample preparation methodology than conventional sample decomposition procedures (e.g., microwave-assisted acid digestion). However, matrix eff...
Source: Analytica Chimica Acta - March 29, 2024 Category: Chemistry Authors: Santiago Mart ínez Lo ïs Morineau Joana Fernandes Fabien Chainet Source Type: research
Method development and validation for the quantitative determination of total flavonoids through the complexation of iron (III) and its application in real sample
Anal Chim Acta. 2024 May 1;1301:342443. doi: 10.1016/j.aca.2024.342443. Epub 2024 Mar 13.ABSTRACTBACKGROUND: The determination of flavonoids in real sample using UV-Vis spectrophotometer commonly uses quercetin and catechin with Al+3 complexing agent as reference materials for the calibration of the instrument. However, getting these standard materials is challenging due to its expense and unavailability in the chemical reserve of the country. Moreover, the Al+3 - quercetin complexation standard method demands high amount of quercetin in spite of its high cost. Hence, developing alternative method that can solve this probl...
Source: Analytica Chimica Acta - March 29, 2024 Category: Chemistry Authors: Sayge Sate Shanko Tolera Seda Badessa Alemu Mekonnen Tura Source Type: research
Nanocluster/metal-organic framework nanosheet-based confined ECL enhancement biosensor for the extracellular vesicle detection
Anal Chim Acta. 2024 May 1;1301:342488. doi: 10.1016/j.aca.2024.342488. Epub 2024 Mar 16.ABSTRACTGastric cancer (GC) was one of the most common cancers with high mortality. The detection of GC peritoneal metastasis had important significance. In this work, we have developed the novel electrochemiluminescence (ECL) biosensor to detect microRNA in GC extracellular vesicle (EV). Firstly, in situ growth of Cu nanocluster (Cu NC) on the metal-organic frameworks (MOFs) nanosheet was achieved successfully. Due to the confinement effect, Cu NCs in the porous structure of Zn MOF possessed the high quantum yield and good stability. ...
Source: Analytica Chimica Acta - March 29, 2024 Category: Chemistry Authors: Fuzhe Ma Wenyan Li Peilin Wang Qiang Ma Source Type: research
A SERS nanocellulose-paper-based analytical device for ultrasensitive detection of Alzheimer's disease
Anal Chim Acta. 2024 May 1;1301:342447. doi: 10.1016/j.aca.2024.342447. Epub 2024 Mar 4.ABSTRACTBACKGROUND: Alzheimer's disease (AD), one of the most prevalent neurodegenerative diseases, results in severe cognitive decline and irreversible memory loss. Early detection of AD is significant to patients for personalized intervention since effective cure and treatment methods for AD are still lacking. Despite the severity of the disease, existing highly sensitive AD detection methods, including neuroimaging and brain deposit-positive lesion tests, are not suitable for screening purposes due to their high cost and complicated ...
Source: Analytica Chimica Acta - March 29, 2024 Category: Chemistry Authors: Wenwen Yuan Hang Yuan Ruibing Li Ruiqi Yong Ivona Mitrovic Eng Gee Lim Sixuan Duan Pengfei Song Source Type: research
Exploring a new generation of bimetallic magnetic ionic liquids with ultra-low viscosity in microextraction that enable direct coupling with high-performance liquid-chromatography
This study represents the first liquid-phase microextraction method using the new generation of low-viscous bimetallic MILs. The proposed MIL-DLLME approach represents 2 important advances with respect to previous methods employing bimetallic MILs: 1) no dispersive solvent is required, and 2) direct injection of the MIL in the HPLC is possible after minor dilution (no back extraction steps are required). Therefore, the microextraction strategy is simple, rapid, and consumes very small amounts of energy.PMID:38553120 | DOI:10.1016/j.aca.2024.342448 (Source: Analytica Chimica Acta)
Source: Analytica Chimica Acta - March 29, 2024 Category: Chemistry Authors: Ra úl González-Martín Sirintorn Jullakan Mar ía J Trujillo-Rodríguez Nabeel Mujtaba Abbasi Shashini De Silva Jared L Anderson Ver ónica Pino Source Type: research
Oriented docking of the template for improved imprinting efficiency toward peptide with modifications
In this report, a facile and one-pot strategy by integrating the in-situ growth of magnetic nanoparticles and reversed phase microemulsion oriented molecularly imprinting strategy to develop magnetic molecular imprinted nanocomposites was proposed. Through self-assembling of the template, it brought up highly ordered and uniform arrangement of the imprinting structure, which offered faster adsorption kinetic as adsorption equilibrium was achived within 15 min, higher adsorption capacity (Qmax = 48.78 ± 1.54 μmol/g) and high affinity (Kd = 127.63 ± 9.66 μM) toward paradigm molecule-adenosine monophosphate (AMP) compared...
Source: Analytica Chimica Acta - March 29, 2024 Category: Chemistry Authors: Juntao Zhou Muhammad Mujahid Ali Wenjing Yu Xianhui Cheng Yujun Gao Lianghai Hu Source Type: research
