BEST4 < sup > + < /sup > cells in the intestinal epithelium
Am J Physiol Cell Physiol. 2024 Apr 1. doi: 10.1152/ajpcell.00042.2024. Online ahead of print.ABSTRACTThe recent development of single cell transcriptomics highlighted the existence of a new lineage of mature absorptive cells in the human intestinal epithelium. This subpopulation is characterized by the specific expression of Bestrophin 4 (BEST4) and of other marker genes including OTOP2, CA7, GUCA2A, GUCA2B, and SPIB. BEST4+ cells appear early in development and are present in all regions of the small and large intestine at a low abundance (<5% of all epithelial cells). Location-specific gene expression profiles in BES...
Source: Am J Physiol Cell Ph... - April 1, 2024 Category: Cytology Authors: Tania Malonga Nathalie Vialaneix Martin Beaumont Source Type: research
Coculture with Colon-26 cancer cells decreases the protein synthesis rate and shifts energy metabolism towards glycolysis dominance in C2C12 myotubes
In this study, we investigated the direct effects of Colon-26 cancer cells on the molecular regulating machinery of muscle mass and its bioenergetics using a coculture system with C2C12 myotubes. Our results demonstrated that coculture with Colon-26 cells induced myotube atrophy and reduced skeletal muscle protein synthesis and its regulating mammalian target of rapamycin complex 1 signal transduction. However, we did not observe any activating effects on protein degradation pathways including ubiquitin-proteasome and autophagy-lysosome systems. From a bioenergetic perspective, coculture with Colon-26 cells decreased the C...
Source: Am J Physiol Cell Ph... - April 1, 2024 Category: Cytology Authors: Yuki Tamura Karina Kouzaki Takaya Kotani Koichi Nakazato Source Type: research
A digoxin derivative that potently reduces intraocular pressure - efficacy and mechanism of action in different animal models
Am J Physiol Cell Physiol. 2024 Apr 1. doi: 10.1152/ajpcell.00617.2023. Online ahead of print.ABSTRACTGlaucoma is a blinding disease. Reduction of intraocular pressure (IOP) is the mainstay of treatment, but current drugs show side-effects or become progressively ineffective, highlighting the need for novel compounds. We have synthesized a family of perhydro-1,4-oxazepine derivatives of digoxin, the selective inhibitor of Na,K-ATPase. The cyclobutyl derivative (DcB) displays strong selectivity for the human a2 isoform and potently reduces IOP in rabbits. These observations appeared consistent with a hypothesis that in cili...
Source: Am J Physiol Cell Ph... - April 1, 2024 Category: Cytology Authors: Veluchamy Amutha Barathi Adriana Katz Shashikant Chaudhary Hoi-Lam Li Daniel M Tal Arie L Marcovich Chi-Wai Do Steven J D Karlish Source Type: research
β-adrenergic signaling drives structural and functional maturation of mouse cardiomyocytes
Am J Physiol Cell Physiol. 2024 Apr 1. doi: 10.1152/ajpcell.00426.2023. Online ahead of print.ABSTRACTCardiac maturation represents the last phase of heart development and is characterized by morphofunctional alterations that optimize the heart for efficient pumping. Its understanding provides important insights into cardiac regeneration therapies. Recent evidence implies that adrenergic signals are involved in the regulation of cardiac maturation, but the mechanistic underpinnings involved in this process are poorly understood. Herein, we explored the role of β-adrenergic receptor (β-AR) activation in determining struct...
Source: Am J Physiol Cell Ph... - April 1, 2024 Category: Cytology Authors: Marcos Eliezeck Itamar Couto Guedes Jesus Sergio A Scalzo Bruno de Lima Sanches Kaoma S C Silva Mateus Costa Thassio Mesquita Cibele Rocha-Resende Raphael E Szawka Silvia Guatimosim Source Type: research
Renal upregulation of NCC counteracts empagliflozin-mediated NHE3 inhibition in normotensive but not in hypertensive male rats
This study examined the effect of empagliflozin (EMPA) on renal tubular sodium transport in normotensive and spontaneously hypertensive rats (SHRs). It also tested the hypothesis that EMPA-mediated PT NHE3 inhibition in normotensive rats is associated with upregulation of distal nephron apical sodium transporters. EMPA administration for 14 days reduced BP in 12-week-old SHRs but not in age-matched Wistar rats. PT NHE3 activity was inhibited by EMPA treatment in both Wistar and SHRs. In Wistar rats, EMPA increased NCC activity, mRNA expression, protein abundance, and phosphorylation levels, but not in SHRs. SHRs showed hig...
Source: Am J Physiol Cell Ph... - April 1, 2024 Category: Cytology Authors: Paulo C Castro Thiago Matheus Santos-Rios Fl ávia Letícia Martins Renato O Crajoinas Marcos Vin ícius Caetano Luc ília M A Lessa Weverton Machado Luchi James A McCormick Adriana C C Girardi Source Type: research
BEST4 < sup > + < /sup > cells in the intestinal epithelium
Am J Physiol Cell Physiol. 2024 Apr 1. doi: 10.1152/ajpcell.00042.2024. Online ahead of print.ABSTRACTThe recent development of single cell transcriptomics highlighted the existence of a new lineage of mature absorptive cells in the human intestinal epithelium. This subpopulation is characterized by the specific expression of Bestrophin 4 (BEST4) and of other marker genes including OTOP2, CA7, GUCA2A, GUCA2B, and SPIB. BEST4+ cells appear early in development and are present in all regions of the small and large intestine at a low abundance (<5% of all epithelial cells). Location-specific gene expression profiles in BES...
Source: Am J Physiol Cell Ph... - April 1, 2024 Category: Cytology Authors: Tania Malonga Nathalie Vialaneix Martin Beaumont Source Type: research
Coculture with Colon-26 cancer cells decreases the protein synthesis rate and shifts energy metabolism towards glycolysis dominance in C2C12 myotubes
In this study, we investigated the direct effects of Colon-26 cancer cells on the molecular regulating machinery of muscle mass and its bioenergetics using a coculture system with C2C12 myotubes. Our results demonstrated that coculture with Colon-26 cells induced myotube atrophy and reduced skeletal muscle protein synthesis and its regulating mammalian target of rapamycin complex 1 signal transduction. However, we did not observe any activating effects on protein degradation pathways including ubiquitin-proteasome and autophagy-lysosome systems. From a bioenergetic perspective, coculture with Colon-26 cells decreased the C...
Source: Am J Physiol Cell Ph... - April 1, 2024 Category: Cytology Authors: Yuki Tamura Karina Kouzaki Takaya Kotani Koichi Nakazato Source Type: research
A digoxin derivative that potently reduces intraocular pressure - efficacy and mechanism of action in different animal models
Am J Physiol Cell Physiol. 2024 Apr 1. doi: 10.1152/ajpcell.00617.2023. Online ahead of print.ABSTRACTGlaucoma is a blinding disease. Reduction of intraocular pressure (IOP) is the mainstay of treatment, but current drugs show side-effects or become progressively ineffective, highlighting the need for novel compounds. We have synthesized a family of perhydro-1,4-oxazepine derivatives of digoxin, the selective inhibitor of Na,K-ATPase. The cyclobutyl derivative (DcB) displays strong selectivity for the human a2 isoform and potently reduces IOP in rabbits. These observations appeared consistent with a hypothesis that in cili...
Source: Am J Physiol Cell Ph... - April 1, 2024 Category: Cytology Authors: Veluchamy Amutha Barathi Adriana Katz Shashikant Chaudhary Hoi-Lam Li Daniel M Tal Arie L Marcovich Chi-Wai Do Steven J D Karlish Source Type: research
β-adrenergic signaling drives structural and functional maturation of mouse cardiomyocytes
Am J Physiol Cell Physiol. 2024 Apr 1. doi: 10.1152/ajpcell.00426.2023. Online ahead of print.ABSTRACTCardiac maturation represents the last phase of heart development and is characterized by morphofunctional alterations that optimize the heart for efficient pumping. Its understanding provides important insights into cardiac regeneration therapies. Recent evidence implies that adrenergic signals are involved in the regulation of cardiac maturation, but the mechanistic underpinnings involved in this process are poorly understood. Herein, we explored the role of β-adrenergic receptor (β-AR) activation in determining struct...
Source: Am J Physiol Cell Ph... - April 1, 2024 Category: Cytology Authors: Marcos Eliezeck Itamar Couto Guedes Jesus Sergio A Scalzo Bruno de Lima Sanches Kaoma S C Silva Mateus Costa Thassio Mesquita Cibele Rocha-Resende Raphael E Szawka Silvia Guatimosim Source Type: research
Renal upregulation of NCC counteracts empagliflozin-mediated NHE3 inhibition in normotensive but not in hypertensive male rats
This study examined the effect of empagliflozin (EMPA) on renal tubular sodium transport in normotensive and spontaneously hypertensive rats (SHRs). It also tested the hypothesis that EMPA-mediated PT NHE3 inhibition in normotensive rats is associated with upregulation of distal nephron apical sodium transporters. EMPA administration for 14 days reduced BP in 12-week-old SHRs but not in age-matched Wistar rats. PT NHE3 activity was inhibited by EMPA treatment in both Wistar and SHRs. In Wistar rats, EMPA increased NCC activity, mRNA expression, protein abundance, and phosphorylation levels, but not in SHRs. SHRs showed hig...
Source: Am J Physiol Cell Ph... - April 1, 2024 Category: Cytology Authors: Paulo C Castro Thiago Matheus Santos-Rios Fl ávia Letícia Martins Renato O Crajoinas Marcos Vin ícius Caetano Luc ília M A Lessa Weverton Machado Luchi James A McCormick Adriana C C Girardi Source Type: research
BEST4 < sup > + < /sup > cells in the intestinal epithelium
Am J Physiol Cell Physiol. 2024 Apr 1. doi: 10.1152/ajpcell.00042.2024. Online ahead of print.ABSTRACTThe recent development of single cell transcriptomics highlighted the existence of a new lineage of mature absorptive cells in the human intestinal epithelium. This subpopulation is characterized by the specific expression of Bestrophin 4 (BEST4) and of other marker genes including OTOP2, CA7, GUCA2A, GUCA2B, and SPIB. BEST4+ cells appear early in development and are present in all regions of the small and large intestine at a low abundance (<5% of all epithelial cells). Location-specific gene expression profiles in BES...
Source: Am J Physiol Cell Ph... - April 1, 2024 Category: Cytology Authors: Tania Malonga Nathalie Vialaneix Martin Beaumont Source Type: research
Coculture with Colon-26 cancer cells decreases the protein synthesis rate and shifts energy metabolism towards glycolysis dominance in C2C12 myotubes
In this study, we investigated the direct effects of Colon-26 cancer cells on the molecular regulating machinery of muscle mass and its bioenergetics using a coculture system with C2C12 myotubes. Our results demonstrated that coculture with Colon-26 cells induced myotube atrophy and reduced skeletal muscle protein synthesis and its regulating mammalian target of rapamycin complex 1 signal transduction. However, we did not observe any activating effects on protein degradation pathways including ubiquitin-proteasome and autophagy-lysosome systems. From a bioenergetic perspective, coculture with Colon-26 cells decreased the C...
Source: Am J Physiol Cell Ph... - April 1, 2024 Category: Cytology Authors: Yuki Tamura Karina Kouzaki Takaya Kotani Koichi Nakazato Source Type: research
A digoxin derivative that potently reduces intraocular pressure - efficacy and mechanism of action in different animal models
Am J Physiol Cell Physiol. 2024 Apr 1. doi: 10.1152/ajpcell.00617.2023. Online ahead of print.ABSTRACTGlaucoma is a blinding disease. Reduction of intraocular pressure (IOP) is the mainstay of treatment, but current drugs show side-effects or become progressively ineffective, highlighting the need for novel compounds. We have synthesized a family of perhydro-1,4-oxazepine derivatives of digoxin, the selective inhibitor of Na,K-ATPase. The cyclobutyl derivative (DcB) displays strong selectivity for the human a2 isoform and potently reduces IOP in rabbits. These observations appeared consistent with a hypothesis that in cili...
Source: Am J Physiol Cell Ph... - April 1, 2024 Category: Cytology Authors: Veluchamy Amutha Barathi Adriana Katz Shashikant Chaudhary Hoi-Lam Li Daniel M Tal Arie L Marcovich Chi-Wai Do Steven J D Karlish Source Type: research
β-adrenergic signaling drives structural and functional maturation of mouse cardiomyocytes
Am J Physiol Cell Physiol. 2024 Apr 1. doi: 10.1152/ajpcell.00426.2023. Online ahead of print.ABSTRACTCardiac maturation represents the last phase of heart development and is characterized by morphofunctional alterations that optimize the heart for efficient pumping. Its understanding provides important insights into cardiac regeneration therapies. Recent evidence implies that adrenergic signals are involved in the regulation of cardiac maturation, but the mechanistic underpinnings involved in this process are poorly understood. Herein, we explored the role of β-adrenergic receptor (β-AR) activation in determining struct...
Source: Am J Physiol Cell Ph... - April 1, 2024 Category: Cytology Authors: Marcos Eliezeck Itamar Couto Guedes Jesus Sergio A Scalzo Bruno de Lima Sanches Kaoma S C Silva Mateus Costa Thassio Mesquita Cibele Rocha-Resende Raphael E Szawka Silvia Guatimosim Source Type: research
Renal upregulation of NCC counteracts empagliflozin-mediated NHE3 inhibition in normotensive but not in hypertensive male rats
This study examined the effect of empagliflozin (EMPA) on renal tubular sodium transport in normotensive and spontaneously hypertensive rats (SHRs). It also tested the hypothesis that EMPA-mediated PT NHE3 inhibition in normotensive rats is associated with upregulation of distal nephron apical sodium transporters. EMPA administration for 14 days reduced BP in 12-week-old SHRs but not in age-matched Wistar rats. PT NHE3 activity was inhibited by EMPA treatment in both Wistar and SHRs. In Wistar rats, EMPA increased NCC activity, mRNA expression, protein abundance, and phosphorylation levels, but not in SHRs. SHRs showed hig...
Source: Am J Physiol Cell Ph... - April 1, 2024 Category: Cytology Authors: Paulo C Castro Thiago Matheus Santos-Rios Fl ávia Letícia Martins Renato O Crajoinas Marcos Vin ícius Caetano Luc ília M A Lessa Weverton Machado Luchi James A McCormick Adriana C C Girardi Source Type: research
