The role of fibronectin in mediating cell migration
Am J Physiol Cell Physiol. 2024 Feb 19. doi: 10.1152/ajpcell.00633.2023. Online ahead of print.ABSTRACTFibronectin (FN) is a major extracellular matrix protein involved in a wide range of physiological processes, including cell migration. These fibronectin mediated cell migration events are essential to processes such as wound repair, cancer metastasis, and vertebrate development. This review synthesizes mainly current literature to provide an overview of the mechanoregulatory role of FN-mediated cell migration. Background on FN structure and role in mechanotransduction is provided. Cell migration concepts are introduced, ...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Jessica H Longstreth Karin Wang Source Type: research
Modulation of prostaglandin transport activity of SLCO2A1 by annexin A2 and S100A10
This study examined PGE2 uptake and ATP release in Anxa2 and/or S100a10 knockout (KO) murine breast C127 cells. Deletion of Slco2a1 decreased PGE2-d4 uptake by wild-type (WT) cells in isotonic medium (290 mOsm/kg H2O). Decreased osmolarity (135 mOsm/kg H2O) stimulated ATP release but didn't affect PGE2 uptake kinetics, showing Km (1280 nM) and Vmax (10.38 pmol/15 sec/mg protein) similar to those in isotonic medium (1227 nM and 10.65 pmol/15 sec/mg protein), respectively, in WT cells. Deletion of Anxa2 associated with loss of S100a10 diminished SLCO2A1-mediated ATP release and uncompetitively inhibited PGE2 uptake with lowe...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Yoshinobu Nakamura Masa-Aki Ito Yukino Hoshino Isao Matsuoka Toshiaki Okada Yasunobu Okada Takeo Nakanishi Source Type: research
The role of fibronectin in mediating cell migration
Am J Physiol Cell Physiol. 2024 Feb 19. doi: 10.1152/ajpcell.00633.2023. Online ahead of print.ABSTRACTFibronectin (FN) is a major extracellular matrix protein involved in a wide range of physiological processes, including cell migration. These fibronectin mediated cell migration events are essential to processes such as wound repair, cancer metastasis, and vertebrate development. This review synthesizes mainly current literature to provide an overview of the mechanoregulatory role of FN-mediated cell migration. Background on FN structure and role in mechanotransduction is provided. Cell migration concepts are introduced, ...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Jessica H Longstreth Karin Wang Source Type: research
Modulation of prostaglandin transport activity of SLCO2A1 by annexin A2 and S100A10
This study examined PGE2 uptake and ATP release in Anxa2 and/or S100a10 knockout (KO) murine breast C127 cells. Deletion of Slco2a1 decreased PGE2-d4 uptake by wild-type (WT) cells in isotonic medium (290 mOsm/kg H2O). Decreased osmolarity (135 mOsm/kg H2O) stimulated ATP release but didn't affect PGE2 uptake kinetics, showing Km (1280 nM) and Vmax (10.38 pmol/15 sec/mg protein) similar to those in isotonic medium (1227 nM and 10.65 pmol/15 sec/mg protein), respectively, in WT cells. Deletion of Anxa2 associated with loss of S100a10 diminished SLCO2A1-mediated ATP release and uncompetitively inhibited PGE2 uptake with lowe...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Yoshinobu Nakamura Masa-Aki Ito Yukino Hoshino Isao Matsuoka Toshiaki Okada Yasunobu Okada Takeo Nakanishi Source Type: research
The role of fibronectin in mediating cell migration
Am J Physiol Cell Physiol. 2024 Feb 19. doi: 10.1152/ajpcell.00633.2023. Online ahead of print.ABSTRACTFibronectin (FN) is a major extracellular matrix protein involved in a wide range of physiological processes, including cell migration. These fibronectin mediated cell migration events are essential to processes such as wound repair, cancer metastasis, and vertebrate development. This review synthesizes mainly current literature to provide an overview of the mechanoregulatory role of FN-mediated cell migration. Background on FN structure and role in mechanotransduction is provided. Cell migration concepts are introduced, ...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Jessica H Longstreth Karin Wang Source Type: research
Modulation of prostaglandin transport activity of SLCO2A1 by annexin A2 and S100A10
This study examined PGE2 uptake and ATP release in Anxa2 and/or S100a10 knockout (KO) murine breast C127 cells. Deletion of Slco2a1 decreased PGE2-d4 uptake by wild-type (WT) cells in isotonic medium (290 mOsm/kg H2O). Decreased osmolarity (135 mOsm/kg H2O) stimulated ATP release but didn't affect PGE2 uptake kinetics, showing Km (1280 nM) and Vmax (10.38 pmol/15 sec/mg protein) similar to those in isotonic medium (1227 nM and 10.65 pmol/15 sec/mg protein), respectively, in WT cells. Deletion of Anxa2 associated with loss of S100a10 diminished SLCO2A1-mediated ATP release and uncompetitively inhibited PGE2 uptake with lowe...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Yoshinobu Nakamura Masa-Aki Ito Yukino Hoshino Isao Matsuoka Toshiaki Okada Yasunobu Okada Takeo Nakanishi Source Type: research
The role of fibronectin in mediating cell migration
Am J Physiol Cell Physiol. 2024 Feb 19. doi: 10.1152/ajpcell.00633.2023. Online ahead of print.ABSTRACTFibronectin (FN) is a major extracellular matrix protein involved in a wide range of physiological processes, including cell migration. These fibronectin mediated cell migration events are essential to processes such as wound repair, cancer metastasis, and vertebrate development. This review synthesizes mainly current literature to provide an overview of the mechanoregulatory role of FN-mediated cell migration. Background on FN structure and role in mechanotransduction is provided. Cell migration concepts are introduced, ...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Jessica H Longstreth Karin Wang Source Type: research
Modulation of prostaglandin transport activity of SLCO2A1 by annexin A2 and S100A10
This study examined PGE2 uptake and ATP release in Anxa2 and/or S100a10 knockout (KO) murine breast C127 cells. Deletion of Slco2a1 decreased PGE2-d4 uptake by wild-type (WT) cells in isotonic medium (290 mOsm/kg H2O). Decreased osmolarity (135 mOsm/kg H2O) stimulated ATP release but didn't affect PGE2 uptake kinetics, showing Km (1280 nM) and Vmax (10.38 pmol/15 sec/mg protein) similar to those in isotonic medium (1227 nM and 10.65 pmol/15 sec/mg protein), respectively, in WT cells. Deletion of Anxa2 associated with loss of S100a10 diminished SLCO2A1-mediated ATP release and uncompetitively inhibited PGE2 uptake with lowe...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Yoshinobu Nakamura Masa-Aki Ito Yukino Hoshino Isao Matsuoka Toshiaki Okada Yasunobu Okada Takeo Nakanishi Source Type: research
The role of fibronectin in mediating cell migration
Am J Physiol Cell Physiol. 2024 Feb 19. doi: 10.1152/ajpcell.00633.2023. Online ahead of print.ABSTRACTFibronectin (FN) is a major extracellular matrix protein involved in a wide range of physiological processes, including cell migration. These fibronectin mediated cell migration events are essential to processes such as wound repair, cancer metastasis, and vertebrate development. This review synthesizes mainly current literature to provide an overview of the mechanoregulatory role of FN-mediated cell migration. Background on FN structure and role in mechanotransduction is provided. Cell migration concepts are introduced, ...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Jessica H Longstreth Karin Wang Source Type: research
Modulation of prostaglandin transport activity of SLCO2A1 by annexin A2 and S100A10
This study examined PGE2 uptake and ATP release in Anxa2 and/or S100a10 knockout (KO) murine breast C127 cells. Deletion of Slco2a1 decreased PGE2-d4 uptake by wild-type (WT) cells in isotonic medium (290 mOsm/kg H2O). Decreased osmolarity (135 mOsm/kg H2O) stimulated ATP release but didn't affect PGE2 uptake kinetics, showing Km (1280 nM) and Vmax (10.38 pmol/15 sec/mg protein) similar to those in isotonic medium (1227 nM and 10.65 pmol/15 sec/mg protein), respectively, in WT cells. Deletion of Anxa2 associated with loss of S100a10 diminished SLCO2A1-mediated ATP release and uncompetitively inhibited PGE2 uptake with lowe...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Yoshinobu Nakamura Masa-Aki Ito Yukino Hoshino Isao Matsuoka Toshiaki Okada Yasunobu Okada Takeo Nakanishi Source Type: research
Myometrium infection decreases TREK1 through NHE1 and increases contraction in pregnant mice
Am J Physiol Cell Physiol. 2024 Feb 12. doi: 10.1152/ajpcell.00598.2023. Online ahead of print.ABSTRACTIntrauterine infection during pregnancy can enhance the uterine contractions. A two-pore K+ channel TREK1 is crucial for maintaining uterine quiescence and reducing contractility, with its properties regulated by pH changes in cell microenvironment. Meanwhile, the sodium hydrogen exchanger 1 (NHE1) plays a pivotal role in modulating cellular pH homeostasis, and its activation increases smooth muscle tension. By establishing an infected mouse model of Escherichia coli (E. coli) and lipopolysaccharide (LPS), we employed Wes...
Source: Am J Physiol Cell Ph... - February 12, 2024 Category: Cytology Authors: Huihui Yu Xingxing Wang Ruixian Tian Xuan Li Chenyi Xu Jiajia Fei Tengteng Li Zongzhi Yin Source Type: research
Elucidation of Pericholangitis and Periductal Fibrosis in Cholestatic Liver diseases via Extracellular Vesicles released by Polarized Biliary Epithelial cells
Am J Physiol Cell Physiol. 2024 Feb 12. doi: 10.1152/ajpcell.00655.2023. Online ahead of print.ABSTRACTCholestatic liver diseases causes inflammation and fibrosis around bile ducts. However, the pathological mechanism has not been elucidated. Extracellular vesicles (EVs) are released from both the basolateral and apical sides of polarized biliary epithelial cells. We aimed to investigate the possibility that EVs released from the basolateral sides of biliary epithelial cells by bile acid stimulation induce inflammatory cells and fibrosis around bile ducts, and it may be involved in the pathogenesis of cholestatic liver dis...
Source: Am J Physiol Cell Ph... - February 12, 2024 Category: Cytology Authors: Keita Maki Tomohiro Katsumi Takumi Hanatani Fumi Uchiyama Fumiya Suzuki Kyoko Hoshikawa Hiroaki Haga Takafumi Saito Yoshiyuki Ueno Source Type: research
Study on the Osmotic Response and Function of < em > Myo < /em > -inositol Oxygenase in euryhaline fish Nile tilapia ( < em > Oreochromis niloticus < /em > )
Am J Physiol Cell Physiol. 2024 Feb 12. doi: 10.1152/ajpcell.00513.2023. Online ahead of print.ABSTRACTTo understand the role of myo-inositol oxygenase (miox) in the osmotic regulation of Nile tilapia, its expression was analyzed in various tissues. The results showed that the expression of miox gene was highest in the kidney, followed by the liver, and was significantly upregulated in the kidney and liver under 1 h hyperosmotic stress. The relative luminescence efficiency of the miox gene transcription starting site (-4617 bp to +312 bp) under hyperosmotic stress was measured. Two fragments (-1640/-1619 and -620/-599) cou...
Source: Am J Physiol Cell Ph... - February 12, 2024 Category: Cytology Authors: Fan Zhang Qiuran Yu Yuxing Huang Yuan Luo Jian-Guang Qin Liqiao Chen Erchao Li Xiaodan Wang Source Type: research
Characterizing the effects of muscle-specific GSK3a/b reduction on murine muscle contractility and metabolism in female mice
Am J Physiol Cell Physiol. 2024 Feb 12. doi: 10.1152/ajpcell.00398.2023. Online ahead of print.ABSTRACTDysregulation of skeletal muscle morphology and metabolism is associated with chronic diseases such as obesity and type 2 diabetes. The enzyme glycogen synthase kinase 3 (GSK3) is highly involved in skeletal muscle physiology and metabolism, acting as a negative regulator of muscle size, strength, adaptive thermogenesis, and glucose homeostasis. Correspondingly, we have shown that partial gene expression knockdown (~40%) of gsk3 specifically in skeletal muscle increases lean mass, reduces fat mass, and activates muscle-ba...
Source: Am J Physiol Cell Ph... - February 12, 2024 Category: Cytology Authors: Briana L Hockey Michael S Finch Jessica L Braun Ahmad Mohammad Lucas A Maddalena Rene Vandenboom Jeffrey A Stuart Brian D Roy Rebecca E K MacPherson Val A Fajardo Source Type: research
Depalmitoylation and Cell Physiology: APT1 as a Mediator of Metabolic Signals
Am J Physiol Cell Physiol. 2024 Feb 12. doi: 10.1152/ajpcell.00542.2023. Online ahead of print.ABSTRACTMore than half of the global population is obese or overweight, especially in Western countries, and this excess adiposity disrupts normal physiology to cause chronic diseases. Diabetes, an adiposity-associated epidemic disease, affects more than 500 million people and cases are projected to exceed one billion before 2050. Lipid excess can impact physiology through the post-translational modification of proteins, including the reversible process of S-palmitoylation. Dynamic palmitoylation cycling requires the S-acylation ...
Source: Am J Physiol Cell Ph... - February 12, 2024 Category: Cytology Authors: Sarah L Speck Xiaochao Wei Clay F Semenkovich Source Type: research
