WISP1 induces the expression of macrophage migration inhibitory factor (MIF) in human lung fibroblasts through Src kinases and EGFR-activated signaling pathways
In this study, we aimed to investigate the WISP1 signalling pathway and its ability to induce the expression of MIF in primary cultures of fibroblasts from normal human lung (HLFs). Our results showed that WISP1 significantly stimulated the expression of MIF in a concentration- and time-dependent fashion. In WISP1-induced expression of MIF, αvβ5 integrin and chondroitin sulphate proteoglycans as well as Src tyrosine kinases, MAP kinases, PI3K/Akt, PKC and NF-κB were involved. WISP1-induced expression of MIF was attenuated in the presence of Src kinases inhibitor PP2 or the MIF tautomerase activity inhibitor, ISO-1. More...
Source: Am J Physiol Cell Ph... - December 25, 2023 Category: Cytology Authors: Maria-Elpida Christopoulou Spyros S Skandalis Eleni Papakonstantinou Daiana Stolz Alexios J Aletras Source Type: research
The slow-release adiponectin analogue ALY688-SR modifies early-stage disease development in the D2.mdx mouse model of Duchenne muscular dystrophy
Am J Physiol Cell Physiol. 2023 Dec 25. doi: 10.1152/ajpcell.00638.2023. Online ahead of print.ABSTRACTFibrosis is associated with respiratory and limb muscle atrophy in Duchenne muscular dystrophy (DMD). Current standard of care partially delays the progression of this myopathy but there remains an unmet need to develop additional therapies. Adiponectin receptor agonism has emerged as a possible therapeutic target to lower inflammation and improve metabolism in mdx mouse models of DMD but the degree to which fibrosis and atrophy are prevented remain unknown. Here, we demonstrate that the recently developed slow-release pe...
Source: Am J Physiol Cell Ph... - December 25, 2023 Category: Cytology Authors: Catherine A Bellissimo Shivam Gandhi Laura N Castellani Mayoorey Murugathasan Luca J Delfinis Arshdeep Thuhan Madison C Garibotti Yeji Seo Irena A Rebalka Henry H Hsu Gary Sweeney Thomas J Hawke Ali A Abdul-Sater Christopher G R Perry Source Type: research
Human Cx50 Isoleucine177 prevents heterotypic docking and formation of functional gap junction channels with Cx43
Am J Physiol Cell Physiol. 2023 Dec 25. doi: 10.1152/ajpcell.00634.2023. Online ahead of print.ABSTRACTThe human lens is an avascular organ, and its transparency is dependent on gap junction (GJ)-mediated microcirculation. Lens GJs are composed of 3 connexins with Cx46 and Cx50 being expressed in lens fibre cells and Cx43 and Cx50 in the epithelial cells. Impairment of GJ communication by either Cx46 or Cx50 mutations has been shown to be one of the main molecular mechanisms of congenital cataracts in mutant carrier families. The docking compatibility and formation of functional heterotypic GJs for human lens connexins hav...
Source: Am J Physiol Cell Ph... - December 25, 2023 Category: Cytology Authors: Robert S Wong Honghong Chen Yi X Li Jessica L Esseltine Peter B Stathopulos Donglin Bai Source Type: research
Sarcomere level mechanics of the fast skeletal muscle of the medaka fish larva
Am J Physiol Cell Physiol. 2023 Dec 25. doi: 10.1152/ajpcell.00530.2023. Online ahead of print.ABSTRACTThe medaka fish (Oryzias latipes) is a vertebrate model used in developmental biology and genetics. Here we explore its suitability as a model for investigating the molecular mechanisms of human myopathies caused by mutations in sarcomeric proteins. To this end, the relevant mechanical parameters of the intact skeletal muscle of wild type medaka are determined using the transparent tail at larval stage 40. Tails were mounted at sarcomere length 2.1 μm in a thermoregulated trough containing physiological solution. Tetanic...
Source: Am J Physiol Cell Ph... - December 25, 2023 Category: Cytology Authors: Matteo Marcello Viviana Cetrangolo Ilaria Morotti Caterina Squarci Marco Caremani Massimo Reconditi Marco Savarese Pasquale Bianco Gabriella Piazzesi Vincenzo Lombardi Bjarne Udd Ivan Conte Vincenzo Nigro Marco Linari Source Type: research
Autologous mitochondrial transplantation in male mice as a strategy to prevent deleterious effects of peripheral ischemia-reperfusion
Conclusion: While further investigations are needed to better characterize underlying mechanisms, mitochondrial transplantation represents a promising strategy in the setting of IR-induced muscular damage.PMID:38145293 | DOI:10.1152/ajpcell.00639.2023 (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - December 25, 2023 Category: Cytology Authors: Laur éline Boutonnet Joris Mallard Anne-Laure Charles Elyse Hucteau Bernard Geny Anne Lejay Antoine Grandperrin Source Type: research
