Normal vision and development in mice with low functional expression of Kir7.1 in heterozygosis for a blindness-producing mutation inactivating the channel
Am J Physiol Cell Physiol. 2024 Feb 26. doi: 10.1152/ajpcell.00597.2023. Online ahead of print.ABSTRACTK+ channel Kir7.1 expressed at the apical membrane of the retinal pigment epithelium (RPE) plays an essential role in retinal function. An isoleucine-to-threonine mutation at position 120 of the protein is responsible for blindness-causing vitreo-retinal dystrophy. We have studied the molecular mechanism of action of Kir7.1-I120T in vitro by heterologous expression and in vivo by in CRISPR-generated knockin mice. Full-size Kir7.1-I120T reaches the plasma membrane but lacks any activity. Analysis of Kir7.1 and the I120T mu...
Source: Am J Physiol Cell Ph... - February 26, 2024 Category: Cytology Authors: Erwin Vera Isabel Cornejo Juan Carlos Henao Felipe Tribi ños Johanna Burgos Francisco V Sepulveda L Pablo Cid Source Type: research
Establishing chronic models of age-related macular degeneration via long-term iron ion overload
Am J Physiol Cell Physiol. 2024 Feb 26. doi: 10.1152/ajpcell.00532.2023. Online ahead of print.ABSTRACTAge-related macular degeneration (AMD) is characterized by the degenerative senescence in the retinal pigment epithelium (RPE) and photoreceptors, which is accompanied by the accumulation of iron ions in the aging retina. However, current models of acute oxidative stress are still insufficient to simulate the gradual progression of AMD. To address this, we established chronic injury models by exposing the aRPE-19 cells, 661W cells, and mouse retina to iron ion overload over time. Investigations at the levels of cell biolo...
Source: Am J Physiol Cell Ph... - February 26, 2024 Category: Cytology Authors: Hao Huang Jingshu Zeng Xinyue Yu Han Du Chaojuan Wen Yan Mao Han Tang Xielan Kuang Wei Liu Huan Yu Huijun Liu Bowen Li Chongde Long Jianhua Yan Huangxuan Shen Source Type: research
Cannabidiol protects C2C12 myotubes against cisplatin-induced atrophy by regulating oxidative stress
Am J Physiol Cell Physiol. 2024 Feb 26. doi: 10.1152/ajpcell.00622.2023. Online ahead of print.ABSTRACTCancer and chemotherapy can both cause cachexia, a complex multi-organ syndrome characterized by body weight loss, due to adipose tissue and skeletal muscle wasting. Changes in body weight and muscle mass are predictive of response to chemotherapy, incidence of treatment-related complications and, ultimately, patient survival, but there are currently still no clear therapeutic strategies to counteract cachexia. Cannabidiol (CBD) is a bioactive phytocannabinoid produced from a plant named Cannabis sativa. In recent years, ...
Source: Am J Physiol Cell Ph... - February 26, 2024 Category: Cytology Authors: Olivier Le Bacquer Phelipe Sanchez V éronique Patrac C ésar Rivoirad Nicolas Saroul Christophe Giraudet Ayhan Kocer Stephane Walrand Source Type: research
Implications of IFN γ SNP rs2069705 in Primary Sjögren's Syndrome: Transcriptional Activation and B Cell Infiltration
CONCLUSION: SNP rs2069705 in the IFNγ gene emerges as a pivotal element in pSS susceptibility, primarily by augmenting IFNγ transcription, activating the JAK/STAT1 pathway, and leading to B lymphocyte infiltration in the exocrine glands.PMID:38406824 | DOI:10.1152/ajpcell.00661.2023 (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - February 26, 2024 Category: Cytology Authors: Xi Chen Min Li Honglin Li Miao Liu Jianrong Su Yuzhu Ji Source Type: research
Normal vision and development in mice with low functional expression of Kir7.1 in heterozygosis for a blindness-producing mutation inactivating the channel
Am J Physiol Cell Physiol. 2024 Feb 26. doi: 10.1152/ajpcell.00597.2023. Online ahead of print.ABSTRACTK+ channel Kir7.1 expressed at the apical membrane of the retinal pigment epithelium (RPE) plays an essential role in retinal function. An isoleucine-to-threonine mutation at position 120 of the protein is responsible for blindness-causing vitreo-retinal dystrophy. We have studied the molecular mechanism of action of Kir7.1-I120T in vitro by heterologous expression and in vivo by in CRISPR-generated knockin mice. Full-size Kir7.1-I120T reaches the plasma membrane but lacks any activity. Analysis of Kir7.1 and the I120T mu...
Source: Am J Physiol Cell Ph... - February 26, 2024 Category: Cytology Authors: Erwin Vera Isabel Cornejo Juan Carlos Henao Felipe Tribi ños Johanna Burgos Francisco V Sepulveda L Pablo Cid Source Type: research
Establishing chronic models of age-related macular degeneration via long-term iron ion overload
Am J Physiol Cell Physiol. 2024 Feb 26. doi: 10.1152/ajpcell.00532.2023. Online ahead of print.ABSTRACTAge-related macular degeneration (AMD) is characterized by the degenerative senescence in the retinal pigment epithelium (RPE) and photoreceptors, which is accompanied by the accumulation of iron ions in the aging retina. However, current models of acute oxidative stress are still insufficient to simulate the gradual progression of AMD. To address this, we established chronic injury models by exposing the aRPE-19 cells, 661W cells, and mouse retina to iron ion overload over time. Investigations at the levels of cell biolo...
Source: Am J Physiol Cell Ph... - February 26, 2024 Category: Cytology Authors: Hao Huang Jingshu Zeng Xinyue Yu Han Du Chaojuan Wen Yan Mao Han Tang Xielan Kuang Wei Liu Huan Yu Huijun Liu Bowen Li Chongde Long Jianhua Yan Huangxuan Shen Source Type: research
Cannabidiol protects C2C12 myotubes against cisplatin-induced atrophy by regulating oxidative stress
Am J Physiol Cell Physiol. 2024 Feb 26. doi: 10.1152/ajpcell.00622.2023. Online ahead of print.ABSTRACTCancer and chemotherapy can both cause cachexia, a complex multi-organ syndrome characterized by body weight loss, due to adipose tissue and skeletal muscle wasting. Changes in body weight and muscle mass are predictive of response to chemotherapy, incidence of treatment-related complications and, ultimately, patient survival, but there are currently still no clear therapeutic strategies to counteract cachexia. Cannabidiol (CBD) is a bioactive phytocannabinoid produced from a plant named Cannabis sativa. In recent years, ...
Source: Am J Physiol Cell Ph... - February 26, 2024 Category: Cytology Authors: Olivier Le Bacquer Phelipe Sanchez V éronique Patrac C ésar Rivoirad Nicolas Saroul Christophe Giraudet Ayhan Kocer Stephane Walrand Source Type: research
The role of fibronectin in mediating cell migration
Am J Physiol Cell Physiol. 2024 Feb 19. doi: 10.1152/ajpcell.00633.2023. Online ahead of print.ABSTRACTFibronectin (FN) is a major extracellular matrix protein involved in a wide range of physiological processes, including cell migration. These fibronectin mediated cell migration events are essential to processes such as wound repair, cancer metastasis, and vertebrate development. This review synthesizes mainly current literature to provide an overview of the mechanoregulatory role of FN-mediated cell migration. Background on FN structure and role in mechanotransduction is provided. Cell migration concepts are introduced, ...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Jessica H Longstreth Karin Wang Source Type: research
Modulation of prostaglandin transport activity of SLCO2A1 by annexin A2 and S100A10
This study examined PGE2 uptake and ATP release in Anxa2 and/or S100a10 knockout (KO) murine breast C127 cells. Deletion of Slco2a1 decreased PGE2-d4 uptake by wild-type (WT) cells in isotonic medium (290 mOsm/kg H2O). Decreased osmolarity (135 mOsm/kg H2O) stimulated ATP release but didn't affect PGE2 uptake kinetics, showing Km (1280 nM) and Vmax (10.38 pmol/15 sec/mg protein) similar to those in isotonic medium (1227 nM and 10.65 pmol/15 sec/mg protein), respectively, in WT cells. Deletion of Anxa2 associated with loss of S100a10 diminished SLCO2A1-mediated ATP release and uncompetitively inhibited PGE2 uptake with lowe...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Yoshinobu Nakamura Masa-Aki Ito Yukino Hoshino Isao Matsuoka Toshiaki Okada Yasunobu Okada Takeo Nakanishi Source Type: research
The role of fibronectin in mediating cell migration
Am J Physiol Cell Physiol. 2024 Feb 19. doi: 10.1152/ajpcell.00633.2023. Online ahead of print.ABSTRACTFibronectin (FN) is a major extracellular matrix protein involved in a wide range of physiological processes, including cell migration. These fibronectin mediated cell migration events are essential to processes such as wound repair, cancer metastasis, and vertebrate development. This review synthesizes mainly current literature to provide an overview of the mechanoregulatory role of FN-mediated cell migration. Background on FN structure and role in mechanotransduction is provided. Cell migration concepts are introduced, ...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Jessica H Longstreth Karin Wang Source Type: research
Modulation of prostaglandin transport activity of SLCO2A1 by annexin A2 and S100A10
This study examined PGE2 uptake and ATP release in Anxa2 and/or S100a10 knockout (KO) murine breast C127 cells. Deletion of Slco2a1 decreased PGE2-d4 uptake by wild-type (WT) cells in isotonic medium (290 mOsm/kg H2O). Decreased osmolarity (135 mOsm/kg H2O) stimulated ATP release but didn't affect PGE2 uptake kinetics, showing Km (1280 nM) and Vmax (10.38 pmol/15 sec/mg protein) similar to those in isotonic medium (1227 nM and 10.65 pmol/15 sec/mg protein), respectively, in WT cells. Deletion of Anxa2 associated with loss of S100a10 diminished SLCO2A1-mediated ATP release and uncompetitively inhibited PGE2 uptake with lowe...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Yoshinobu Nakamura Masa-Aki Ito Yukino Hoshino Isao Matsuoka Toshiaki Okada Yasunobu Okada Takeo Nakanishi Source Type: research
The role of fibronectin in mediating cell migration
Am J Physiol Cell Physiol. 2024 Feb 19. doi: 10.1152/ajpcell.00633.2023. Online ahead of print.ABSTRACTFibronectin (FN) is a major extracellular matrix protein involved in a wide range of physiological processes, including cell migration. These fibronectin mediated cell migration events are essential to processes such as wound repair, cancer metastasis, and vertebrate development. This review synthesizes mainly current literature to provide an overview of the mechanoregulatory role of FN-mediated cell migration. Background on FN structure and role in mechanotransduction is provided. Cell migration concepts are introduced, ...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Jessica H Longstreth Karin Wang Source Type: research
Modulation of prostaglandin transport activity of SLCO2A1 by annexin A2 and S100A10
This study examined PGE2 uptake and ATP release in Anxa2 and/or S100a10 knockout (KO) murine breast C127 cells. Deletion of Slco2a1 decreased PGE2-d4 uptake by wild-type (WT) cells in isotonic medium (290 mOsm/kg H2O). Decreased osmolarity (135 mOsm/kg H2O) stimulated ATP release but didn't affect PGE2 uptake kinetics, showing Km (1280 nM) and Vmax (10.38 pmol/15 sec/mg protein) similar to those in isotonic medium (1227 nM and 10.65 pmol/15 sec/mg protein), respectively, in WT cells. Deletion of Anxa2 associated with loss of S100a10 diminished SLCO2A1-mediated ATP release and uncompetitively inhibited PGE2 uptake with lowe...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Yoshinobu Nakamura Masa-Aki Ito Yukino Hoshino Isao Matsuoka Toshiaki Okada Yasunobu Okada Takeo Nakanishi Source Type: research
The role of fibronectin in mediating cell migration
Am J Physiol Cell Physiol. 2024 Feb 19. doi: 10.1152/ajpcell.00633.2023. Online ahead of print.ABSTRACTFibronectin (FN) is a major extracellular matrix protein involved in a wide range of physiological processes, including cell migration. These fibronectin mediated cell migration events are essential to processes such as wound repair, cancer metastasis, and vertebrate development. This review synthesizes mainly current literature to provide an overview of the mechanoregulatory role of FN-mediated cell migration. Background on FN structure and role in mechanotransduction is provided. Cell migration concepts are introduced, ...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Jessica H Longstreth Karin Wang Source Type: research
Modulation of prostaglandin transport activity of SLCO2A1 by annexin A2 and S100A10
This study examined PGE2 uptake and ATP release in Anxa2 and/or S100a10 knockout (KO) murine breast C127 cells. Deletion of Slco2a1 decreased PGE2-d4 uptake by wild-type (WT) cells in isotonic medium (290 mOsm/kg H2O). Decreased osmolarity (135 mOsm/kg H2O) stimulated ATP release but didn't affect PGE2 uptake kinetics, showing Km (1280 nM) and Vmax (10.38 pmol/15 sec/mg protein) similar to those in isotonic medium (1227 nM and 10.65 pmol/15 sec/mg protein), respectively, in WT cells. Deletion of Anxa2 associated with loss of S100a10 diminished SLCO2A1-mediated ATP release and uncompetitively inhibited PGE2 uptake with lowe...
Source: Am J Physiol Cell Ph... - February 19, 2024 Category: Cytology Authors: Yoshinobu Nakamura Masa-Aki Ito Yukino Hoshino Isao Matsuoka Toshiaki Okada Yasunobu Okada Takeo Nakanishi Source Type: research
