Rapid spread of unusual G9P[8] human rotavirus strains possessing NSP4 genes of the E2 genotype in Japan
Jpn J Infect Dis. 2022 Apr 28. doi: 10.7883/yoken.JJID.2022.020. Online ahead of print.ABSTRACTThe emergence of unusual G9P[8]-E2 human rotaviruses in the Tokyo metropolis, Japan in 2018 has been reported. During rotavirus strain surveillance in different regions of Japan (Mie, Okayama, and Chiba prefectures), G9P[8]-E2 strains were detected in diarrheic children in all three prefectures. Here, we characterized the whole genomes of seven representative G9P[8]-E2 strains. On full-genome-based analysis, the seven study strains exhibited a unique genotype configuration having the NSP4 gene of genogroup 2 in a genogroup 1 genomic backbone: G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E2-H1. This genotype constellation is shared by Tokyo G9P[8]-E2 strains. Phylogenetic analysis showed that all the 11 genes, except the NSP4 one, of the seven study strains appeared to have originated from co-circulating Wa-like G9P[8]-E1 strains. On the other hand, the NSP4 gene appeared to have originated from co-circulating DS-1-like G2P[4]-E2 strains. Thus, these study G9P[8]-E2 strains appeared to be derived through reassortment between G9P[8]-E1 and G2P[4]-E2 strains in Japan. Notably, the seven study G9P[8]-E2 strains and Tokyo G9P[8]-E2 strains were revealed to have 11-segment genomes almost indistinguishable from one another in their sequences (99.3-100%), indicating all these G9P[8]-E2 strains had a common origin. To our knowledge, this is the first description of the rapid spread of G9P[8]-E2 strains acro...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Saori Fukuda Yuki Akari Riona Hatazawa Manami Negoro Takaaki Tanaka Kazutoyo Asada Haruna Nakamura Katsumi Sugiura Masakazu Umemoto Haruo Kuroki Hiroaki Ito Shigeki Tanaka Mitsue Ito Tomihiko Ide Takayuki Murata Kiyosu Taniguchi Shigeru Suga Hajime Kamiya Source Type: research