GSE62085 The Pitt-Hopkins syndrome/schizophrenia susceptibility gene TCF4 regulates human neural progenitor cell proliferation

Contributors : Matthew J Hill ; Katherinne Navarrete ; Aleksandra Maruszak ; Gemma M McLaughlin ; Richard Killick ; James Docker ; Ricardo Sainz Fuertes ; Brenda P Williams ; Nicholas J BraySeries Type : Expression profiling by arrayOrganism : Homo sapiensTranscription factor 4 (TCF4, also known as E2-2, SEF2, ITF2 and bHLHb19) is an E-protein basic helix-loop-helix transcription factor that is highly expressed in foetal as well as adult human brain. Common variants in the TCF4 gene show genome-wide significant association with schizophrenia and the eye disease Fuchs corneal dystrophy, while rare TCF4 deletions and loss-of-function point mutations cause Pitt-Hopkins Syndrome, a developmental disorder associated with severe intellectual disability. However, the functions of TCF4 during early human brain development have, to date, been largely unknown. In the present study, we performed genome-wide gene expression profiling of two neural progenitor cell lines derived from human foetal brain following knockdown of TCF4 by RNA interference. We found that TCF4 knockdown resulted in altered expression of genes involved in cell cycling in both cell lines, but that the expression of modules of correlated cell cycle genes differed between them in the direction in which they correlated with TCF4 expression. Consistent with the gene expression data, TCF4 siRNA altered the proliferation of both neural progenitor lines, with one line showing decreased proliferation and the other show...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research