Assessment of Ascorbic Acid Niosomes as a Potential Detoxifier in Oxidative Stress-induced HEK-293 Cells by Arsenic trioxide

Background and objectives: Arsenic (As) as an environmental contaminant has many risks to human health. Vitamin C (Vit C), as a powerful antioxidant and water-soluble vitamin, protects cells and tissues against oxidation and has a wide range of healing properties. In this regard, the current study aimed to formulate a suitable niosome of ascorbic acid (Vit C) and compare it with vitamin C in preventing As-induced toxicity in HEK-293 cells.Methods: Various formulas of the niosomes of Vit C were prepared by C-SPAN mixed with cholesterol. The physicochemical characteristics of niosomal formulations, including load size, zeta-potential, and the drug release profile were evaluated in HEK-293 cells. Then, OS biomarkers such as total reactive oxygen species (ROS), malondialdehyde (MDA), catalase (CAT), antioxidant capacity (TAC), and superoxide dismutase (SOD) activities were used for determining the protective effect of the niosomes of vitamin C compared with vitamin C against As -induced toxicity.Key findings: Based on the results, the particle size and zeta potential of the optimal niosomes of vitamin C were 163.2±6.1 nm and 23.3±3.5 mV, respectively. As increased ROS and MDA level while decreasing the content of CAT, TAC, and SOD activities in the Hek-293 cell line. Finally, the niosome of Vit C decreased OS status whereas increasing anti-oxidative property compared to Vit C.Significance: These results demonstrated that the niosome of Vit C was more effecti...
Source: Iranian Journal of Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research