Proteomic insights of chitosan mediated inhibition of Fusarium oxysporum f. sp. cucumerinum

This study gives a snapshot of the molecular basis of fungal inhibition by chitosan resulting in disease resistance in cucumber plants after inoculation with chitosan-treated FOC by explaining how chitosan restricted disease severity (i.e., down-regulating the plant cell wall degrading enzymes, FOC self-attack, hindering FOC structural and functional protein biosynthesis and DNA biosynthesis and affecting FOC transporter proteins). This study contributes to putting more weight on using the bioactive natural compound chitosan as an antifungal material instead of applying chemical fungicides in agriculture. SIGNIGICANCE: Fusarium oxysporum f. sp. cucumerinum (FOC) infects cucumber plants, causing significant yield losses. Chitosan is a natural biodegradable compound applied in agriculture for its antifungal properties. The effect of chitosan on FOC disease severity was shown to be significantly lower (5.3%) when compared to FOC not treated with chitosan. To understand the inhibitory mechanism of chitosan against FOC, a comprehensive proteomic study was carried out for identification of chitosan responsive proteins (CRPs) from the mycelia of chitosan treated FOC that led to the identification of 62 differentially expressed CRPs. Through this proteomics study, we identified Chitosan Responsive Proteins (CRPs) in FOC that are associated with diverse functions bestowing disease inhibition in cucumber. This study gives the picture of what happens inside the fungus after chitosan tre...
Source: Journal of Proteomics - Category: Biochemistry Authors: Source Type: research