Effect of expression of PPARG, DNM2L, RRAD, and LINGO1 on broiler chicken breast muscle satellite cell function

The objective of this study was to determine the expression of the adipogenic genes PPARG, DNM2L, RRAD, and LINGO1 in commercial Ross 708 (708) and Randombred (RBch) satellite cells. RBch satellite cells are from commercial 1995 broilers before WB and 708 broilers are a modern commercial line. In general, expression of these genes was different between the 708 and RBch satellites during proliferation and differentiation. Expression of PPARG and RRAD were both significantly increased during both proliferation and differentiation in the 708 cells (P ≤ 0.05). Knocking down the expression of these genes with small interfering RNAs did not greatly affect either proliferation or differentiation. Lipid accumulation was affected by the knockdown of these genes with significant line effects from 48 h of proliferation through 72 h of differentiation. In general, 708 satellite cells had higher lipid levels. Knockdown treatment effect was specific to each gene. The results demonstrate that lipid biosynthesis has been affected in breast muscle satellite cells which may contribute to the increased lipid deposition in modern day commercial broiler chickens.PMID:35278723 | DOI:10.1016/j.cbpa.2022.111186
Source: Comparative Biochemistry and Physiology. Part A, Molecular and integrative physiology. - Category: Physiology Authors: Source Type: research