Mitigation of Toxoplasma gondii-induced ileitis by Trichinellaspiralis infection pinpointing immunomodulation

AbstractThe current study sought to investigate the potential role ofTrichinellaspiralis infection in the treatment ofT. gondii-induced ileitis. Forty male Swiss albino mice were divided into four groups:a normal control group Igiven only phosphate-buffered saline (PBS), Group II givenPBS for 28  days then infected withT. gondii cysts for the induction of gastroenteritis, Group III infected only withT. spiralis larvae, and Group IV concurrently infected withT. spiralis larvae, then 28  days post infection, enteritis was induced by oral inoculation withT. gondii cysts. Histopathologicaland immunohistochemicalassessmentswere performed to determine the levels of inflammatory markers nuclear factor- κB (NF-κB) and myeloperoxidase in the ileum samples.Theconcentrations of cytokinesIFN-γ and IL10 were measured in successive serum samples. Histological assessment revealed severe inflammatory infiltrations in ileum samples ofT. gondiimonoinfected mice. In addition, the immunological assessment revealed elevated levels of IFN- γ and decreased IL10 concentrations in blood samples. Clear improvement of inflammations, besidesthe decreasedlevels of IFN-γ and increased IL10 concentrations in blood samples were detected inT. spiraliscoinfected animals.Theileal tissue revealed elevated expression of (NF- κB) and myeloperoxidase signaling, all of which were mitigated byT. spiralis coinfection. There is a possibility that regulatory T cells are immunomodulated, releasing anti-inflammat...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research